niman Posted February 5, 2016 Report Share Posted February 5, 2016 Active Zika found in saliva and urineBy James GallagherHealth editor, BBC News websitehttp://www.bbc.com/news/health-35501491 Link to comment Share on other sites More sharing options...
niman Posted February 5, 2016 Author Report Share Posted February 5, 2016 43 minutes ago From the sectionHealthImage copyrightGetty ImagesActive Zika virus has been detected in the saliva and urine of patients, Brazilian scientists say.The finding does not mean the virus can be readily transmitted through the bodily fluids.The main method of infection is via mosquito bites, but scientists are investigating all other possibilities.While Zika infection is normally mild, it has been linked to thousands of suspected birth defects.Traces of Zika have been detected in saliva and urine during the 2013 outbreak in French Polynesia, but the Brazilian authorities say this is the first time "active" virus has been detected.Paulo Gadelha, the head of the Fiocruz Institute which is part of the Ministry of Health, said: "The presence of the active Zika virus has been found in saliva and urine."But that does not mean there is a capacity for transmission through saliva and urine."The potential risk of transmission through bodily fluids was highlighted in the US where the Centers for Disease Control believe a case was spread through sex.However, there have been only two suspected cases of sexual transmission of Zika.More on the Zika crisis:What you need to know Key questions answered about the virus and its spreadKey unanswered questions The many things we do not know about ZikaTravel advice Countries affected and what you should doThe mosquito behind spread of virus What we know about the insectAbortion dilemma Laws and practices in Catholic Latin AmericaProf Jonathan Ball, a virologist at the University of Nottingham, told the BBC: "Because we can detect a virus in a particular body fluid it does not mean that it will become an important source of virus for transmission to humans."At the peak of virus replication in the blood, virus can often be detected in other body fluids, but the levels of virus are often much lower and there is no obvious or efficient means for the virus to get from that bodily fluid into another person's bloodstream."The risks of different modes of infection are still unclear.But experts say that the million-plus suspected cases in the Americas have been contained to areas where the mosquito is found, suggesting it does not spread easily through other means.Brazil has seen 4,783 suspected cases of babies born with small brains, although only 404 have been confirmed, 709 have been rejected and 3,670 are still being investigated.Follow James on Twitter. Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 LOCUS KU926309 10795 bp ss-RNA linear VRL 21-MAR-2016 DEFINITION Zika virus isolate Rio-U1, complete genome. ACCESSION KU926309 VERSION KU926309.1 GI:1006593051 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus. REFERENCE 1 (bases 1 to 10795) AUTHORS Bonaldo,M.C., Ribeiro,I.P., Lima,N.S., Santos,A.A.C., Raphael,L.M.S., Cruz,S.O.D., Mello,I.S., Furtado,N.D., Moura,E.E., Castro,M.G., Gerber,A.L., Almeida,L.G.P., Lourenco-de-Oliveira,R., Vasconcelos,A.T.R. and Brasil,P. TITLE Isolation of infective Zika virus from urine and saliva of patients in Brazil JOURNAL Unpublished REFERENCE 2 (bases 1 to 10795) AUTHORS Bonaldo,M.C., Ribeiro,I.P., Lima,N.S., Santos,A.A.C., Raphael,L.M.S., Cruz,S.O.D., Mello,I.S., Furtado,N.D., Moura,E.E., Castro,M.G., Gerber,A.L., Almeida,L.G.P., Lourenco-de-Oliveira,R., Vasconcelos,A.T.R. and Brasil,P. TITLE Direct Submission JOURNAL Submitted (16-MAR-2016) Laboratorio Nacional de Bioinformatica / Laboratorio Nacional de Computacao Cientifica, Laboratorio de Biologia Molecular de Flavivirus / Fundacao Oswaldo Cruz, Rua Getulio Vargas, 333, Petropolis, RJ 25651075, Brazil COMMENT ##Assembly-Data-START## Assembly Method :: Ray v. 2.2 Coverage :: 8000 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10795 /organism="Zika virus" /mol_type="genomic RNA" /isolate="Rio-U1" /isolation_source="urine" /host="Homo sapiens" /db_xref="taxon:64320" /country="Brazil" /collection_date="14-Jan-2016" /note="passage details: Vero cells"http://www.ncbi.nlm.nih.gov/nuccore/KU926309 Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 LOCUS KU926310 10805 bp ss-RNA linear VRL 21-MAR-2016 DEFINITION Zika virus isolate Rio-S1, complete genome. ACCESSION KU926310 VERSION KU926310.1 GI:1006593054 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus. REFERENCE 1 (bases 1 to 10805) AUTHORS Bonaldo,M.C., Ribeiro,I.P., Lima,N.S., Santos,A.A.C., Raphael,L.M.S., Cruz,S.O.D., Mello,I.S., Furtado,N.D., Moura,E.E., Castro,M.G., Gerber,A.L., Almeida,L.G.P., Lourenco-de-Oliveira,R., Vasconcelos,A.T.R. and Brasil,P. TITLE Isolation of infective Zika virus from urine and saliva of patients in Brazil JOURNAL Unpublished REFERENCE 2 (bases 1 to 10805) AUTHORS Bonaldo,M.C., Ribeiro,I.P., Lima,N.S., Santos,A.A.C., Raphael,L.M.S., Cruz,S.O.D., Mello,I.S., Furtado,N.D., Moura,E.E., Castro,M.G., Gerber,A.L., Almeida,L.G.P., Lourenco-de-Oliveira,R., Vasconcelos,A.T.R. and Brasil,P. TITLE Direct Submission JOURNAL Submitted (16-MAR-2016) Laboratorio Nacional de Bioinformatica / Laboratorio Nacional de Computacao Cientifica, Laboratorio de Biologia Molecular de Flavivirus / Fundacao Oswaldo Cruz, Rua Getulio Vargas, 333, Petropolis, RJ 25651075, Brazil COMMENT ##Assembly-Data-START## Assembly Method :: Ray v. 2.2.0 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10805 /organism="Zika virus" /mol_type="genomic RNA" /isolate="Rio-S1" /isolation_source="saliva" /host="Homo sapiens" /db_xref="taxon:64320" /country="Brazil" /collection_date="29-Jan-2016" /note="passage details: Vero cells"http://www.ncbi.nlm.nih.gov/nuccore/KU926310 Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 S1 Table Clinical symptomsPatient1234Onset date01/11/1601/24/1601/22/1601/31/16Days after symptoms onset *3251Days after rash onset *<1**111Rash duration5 days6 days2 days2 daysRash typeMacularMacular and maculo-papularMaculo-papularMaculo-papularLow grade fever(duration)-+ (2 days)+ (4 days)-Headache--+-Retro-orbital pain--+-Photophobia--NI-Fatigue/ malaise-++-Myalgia-++-Arthralgia(region)+ (wrist and elbows)-+ (large and small joints)-Arthritis----Anorexia--+-Nausea/ vomiting--+-Diarrhea----Abdominal pain----Dysuria----Bleeding/ petechia----Dizziness/ light headedness----Pruritus+-++Paresthesias+---Conjunctivitis----Edema(local/site)+ (hands)---Lymphadenopathy(local/ site)----EnanthemNI-NI-Respiratory symptoms----Jaundice----Seizures----* Regarding the date of sample collection; **the collection date was in the first day of rash manifestation S2 TableClinical symptomsPatient56789Onset date01/26/1601/26/1601/31/1601/31/1601/28/16Days after sypmtons onset *23125Days after rash onset *2<1122Rash duration 20 days 4 days5 days6 days3 daysRash typeMacularMaculo-papularMaculo-papularMacular and maculo-papularMaculo-papularLow grade fever(duration)-+ (1 day)-+ (2 days)-Headache+--++Retro-orbital pain+--++Photophobia+---+Fatigue/ malaise++-+-Myalgia++--+Arthralgia(region)-+ (wrists, ankles, knees, hands)-+ (large and small joints)-Arthritis-----Anorexia++---Nausea/ vomiting+----Diarrhea+----Abdominal pain-+---Dysuria-----Bleeding/ petechia ----Dizziness/ light headedness+----Pruritus+++++Paresthesias++---Conjunctivitis++--+Edema+ (feet and hands) +(hands)-+-Lymphadenopathy(local/ site)+(Cervical and auricular)---+(cervical)Enanthem-----Respiratory symptoms +--+-Jaundice-----Seizures-----* Regarding the date of sample collection; **the collection date was in the first day of rash manifestation S3 Table Social-demographic dataPatient1234GenderfemalefemalemalefemaleAge36302442Gestational age (weeks)1833NA21Family members illness--+-Partner illness--NI-Repellent spray use++NI+Previous DENV infection+-NI-Domicile in Rio de Janeiro StateDuque de CaxiasNova IguaçuRio de JaneiroDuque de CaxiasNA – not applicable; NI- not informed S4 Table Social demographic dataPatient56789Gender femalemalefemalefemalefemaleAge3068202722Gestational age (weeks)NANA172120Family members illness-+---Partner illness-----Repellent spray use+-+++Previous DENV infection++-+-Domicile in Rio de Janeiro StateRio de JaneiroRio de JaneiroRio de JaneiroDuque de CaxiasRio de JaneiroNA – not applicable; NI- not informed Link to comment Share on other sites More sharing options...
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