niman Posted July 21, 2016 Report Share Posted July 21, 2016 07/21/2016Fiocruz identifies Culex mosquito with potential transmission of Zika virus By: Solange Argenta (Fiocruz Pernambuco)Unpublished research conducted by the Oswaldo Cruz Foundation (Fiocruz) detected the presence of Zika virus in mosquito Culex quinquefasciatus (the popular muriçoca or house mosquito) collected in the city of Recife.This finding confirms the species as a potential vector of the virus that zika, a situation which, according to scientific literature, had not been proven to date.http://portal.fiocruz.br/pt-br/content/fiocruz-identifica-mosquito-culex-com-potencial-de-transmissao-do-virus-zika-no-recife Link to comment Share on other sites More sharing options...
niman Posted July 21, 2016 Author Report Share Posted July 21, 2016 07/21/2016Fiocruz identifies Culex mosquito with potential transmission of Zika virus By: Solange Argenta (Fiocruz Pernambuco)Unpublished research conducted by the Oswaldo Cruz Foundation (Fiocruz) detected the presence of Zika virus in mosquito Culex quinquefasciatus (the popular muriçoca or house mosquito) collected in the city of Recife.This finding confirms the species as a potential vector of the virus that zika, a situation which, according to scientific literature, had not been proven to date.The survey was conducted by Fiocruz Pernambuco in the metropolitan area of Recife, where the population of Culex quinquefasciatus is about twenty times greater than the population of Aedes aegypti . Preliminary results of field research identified the presence of Culex quinquefasciatus naturally infected zika virus in three of the 80 pools * (groups) of mosquitoes analyzed to date. In two of these samples they were not fed mosquitoes, indicating that the virus was widespread in the insect body and not in a recent power in an infected host.The technique used in the experiment was quantitative RT-PCR based detection of RNA (genetic material) virus. The material of these positive pools was used to isolate the circulating virus strains in Reef, in cell culture, where it was observed cytopathic effect induced in the cells - i.e., destruction or damage of vero cells was observed, which proves the presence viral activity.The collection of mosquitoes was made based on the addresses of the reported cases of zika in the cities of Recife and Arcoverde, obtained from the Health Department of Pernambuco State (SES-PE). The total number of mosquitoes examined in the study was approximately 500. The aim of the project is to compare the role of some species of mosquitoes in Brazil of arbovirus transmission. Priority was given to Zika virus because the epidemic of the disease in Brazil and its connection with microcephaly. In the laboratory stage, in order to investigate the vector competence of the species Culex quinquefasciatus and Aedes aegypti , mosquitoes were fed a mixture of blood and viruses, allowing the monitoring of pathogen replication process within the insect. There were two mosquito infections each infection with two different virus concentrations (104 and 106) of Ziku BRPE243 / 2015 lineage. "The smallest simulates viremia condition of a real patient. Then the mosquitoes were collected at different times: at time zero (after infection), three days, seven days, 11 and 15 days after virus infection, "says Constance Ayres, coordinator of the study.A control group with mosquitoes fed on blood without the virus, was also maintained. Each mosquitoes was dissected to extract the intestine, and salivary gland tissues that present barriers to the development of the virus. The procedure takes place so that, if species vector is not at a given moment the development of the virus is blocked by the mosquito.However, if it is vector, virus replication occurs, spreads in the insect body and just infecting the salivary gland, from which can be transmitted to other hosts during the blood meal, the release of saliva containing virus.According Constancia, from the third day after artificial feeding has been possible to detect the presence of the virus in the salivary glands of both species of mosquito investigated. After seven days, it was observed the peak of infection in the salivary glands which was confirmed by electron microscopy.Besides the detection of virus in these tissues (intestine , and salivary gland), saliva samples were investigated expelled by mosquitoes infected by quantitative RT-PCR. The viral load found in two species ( Aedes aegypti and Culex quinquefasciatus ) was similar. From the data obtained will require additional studies to assess the potential participation of Culex in the spread of Zika virus and its role in the epidemic. The current study is very important, since the vector control measures are different. Until the results of new evidence, the control policy Zika epidemic still guided by the same guidelines, with its central focus on the control of Aedes aegypti . Link to comment Share on other sites More sharing options...
niman Posted July 21, 2016 Author Report Share Posted July 21, 2016 Zika is found in common Culex mosquitos, signaling a potentially larger risk “Culex is a much more abundant species,” Ayres told a news conference in Rio de Janeiro on Thursday, where she presented theresults.The researchers analyzed 456 female Culex mosquitoes, which they divided into 80 “pools” or sample groups of between one and 10 mosquitoes each. They found Zika-infected insects in three of these pools.Ayres said the research proved that Culex can transmit Zika and that it could have played a role in the rapid spread of the disease in Brazil.“It can transmit Zika. What we need to know now is which species is the most important — if Culex is the primary vector or the secondary vector. We need to do more research,” she said.Paulo Gadelha, president of the Oswaldo Cruz Foundation, expressed some caution during Thursday’s news conference. He said that a study at the institution in Rio by researcher Ricardo Lourenço-de-Oliveira had so far failed to find Zika in around 750 Culex mosquitoes.“It could be that mosquitoes circulating in the Northeast [of Brazil] have more interaction,” he said, stressing that more research was needed.Gadilha said the discovery did not change the low risk of Zika during Brazil’s Olympics, which open in Rio on Aug. 5 during the country’s cooler winter season. “The risk you have of Zika during this period is very, very remote,” he said. Peter Hotez, dean of the National School of Tropical Medicine at Baylor College of Medicine in Texas said that if researchers have found Zika in Culex mosquitoes only a handful of times, “it’s hard to know what that means; it may not be a finding of great biological significance.” But if they are detecting the virus in Culex mosquitoes in large numbers and on a consistent basis, “That would be a game changer.”Hotez was skeptical that Culex mosquitoes are going to start spreading Zika in a significant way. “So far, every place we’ve seen Zika has been a place where you have Aedes aegypti mosquitoes,” he said. “There no reason why one would have to speculate that another mosquito vector is involved.”https://www.washingtonpost.com/world/the_americas/zika-is-found-in-common-culex-mosquitos-signaling-a-potentially-larger-risk/2016/07/21/e1b37e0e-4f6f-11e6-bf27-405106836f96_story.html Link to comment Share on other sites More sharing options...
“Culex is a much more abundant species,” Ayres told a news conference in Rio de Janeiro on Thursday, where she presented theresults.The researchers analyzed 456 female Culex mosquitoes, which they divided into 80 “pools” or sample groups of between one and 10 mosquitoes each. They found Zika-infected insects in three of these pools.Ayres said the research proved that Culex can transmit Zika and that it could have played a role in the rapid spread of the disease in Brazil.“It can transmit Zika. What we need to know now is which species is the most important — if Culex is the primary vector or the secondary vector. We need to do more research,” she said.Paulo Gadelha, president of the Oswaldo Cruz Foundation, expressed some caution during Thursday’s news conference. He said that a study at the institution in Rio by researcher Ricardo Lourenço-de-Oliveira had so far failed to find Zika in around 750 Culex mosquitoes.“It could be that mosquitoes circulating in the Northeast [of Brazil] have more interaction,” he said, stressing that more research was needed.Gadilha said the discovery did not change the low risk of Zika during Brazil’s Olympics, which open in Rio on Aug. 5 during the country’s cooler winter season. “The risk you have of Zika during this period is very, very remote,” he said. Peter Hotez, dean of the National School of Tropical Medicine at Baylor College of Medicine in Texas said that if researchers have found Zika in Culex mosquitoes only a handful of times, “it’s hard to know what that means; it may not be a finding of great biological significance.” But if they are detecting the virus in Culex mosquitoes in large numbers and on a consistent basis, “That would be a game changer.”Hotez was skeptical that Culex mosquitoes are going to start spreading Zika in a significant way. “So far, every place we’ve seen Zika has been a place where you have Aedes aegypti mosquitoes,” he said. “There no reason why one would have to speculate that another mosquito vector is involved.”https://www.washingtonpost.com/world/the_americas/zika-is-found-in-common-culex-mosquitos-signaling-a-potentially-larger-risk/2016/07/21/e1b37e0e-4f6f-11e6-bf27-405106836f96_story.html
niman Posted July 22, 2016 Author Report Share Posted July 22, 2016 Zika found in common house mosquitoes in BrazilBy Debra Goldschmidt and Vasco Cotovio, CNN Updated 1:19 AM ET, Fri July 22, 2016 Everything you need to know about Zika 01:42Story highlightsMore research is needed to see how this will impact the spread of ZikaThere are many different types of Culex mosquitoes, not all can transmit the disease (CNN)Researchers in Brazil announced Thursday the "presence of the Zika virus" in Culex mosquitoes (the common house mosquito) in the eastern city of Recife. These findings were released with a word of caution, saying "the obtained data will require additional studies in order to assess the potential participation of Culex in the spread of Zika and its role in the epidemic." Zika in Florida: 2nd possible non-travel case investigatedResearchers collected 500 mosquitoes and found the virus in three pools of mosquitoes. Each pool contains between one and ten mosquitoes. The presence of the virus in these mosquitoes does not mean they can transmit the virus.Before this study was completed by the Oswaldo Cruz Foundation (Fiocruz), Zika was thought to solely by carried by the Aedes aegypti mosquito. Culex mosquitoes are "twenty times greater than the population of Aedes Aegypti" in the Recife metropolitan area, according to the study.CNN Map Recife Rio de Janeiro Fiocruz is a public institution attached to the Brazilian Ministry of Health. It is one of Brazil's oldest and most respected health care institutions that has been at the forefront of the fight against Zika. Zika Virus Infection Fast Facts Tom Skinner, U. S. Centers for Disease Control and Prevention's Senior Press Officer told CNN Thursday in response to the study's findings, "the study would need to be replicated to have a better understanding of possible implications. Body of scientific evidence to date clearly points to Aedes being the primary vector implicated in Zika outbreaks."Map: Tracking the Zika virus First case of female-to-male Zika transmissionThere are many different types of Culex mosquitoes. This study found the virus in Culex quinquefasciatus mosquitoes. A U.S. study published earlier this week in the journal Emerging Infectious Diseases, found a different type of Culex mosquito was not capable to transmitting the Zika virus in a lab setting. The mosquitoes that changed historyThey concluded that this type of mosquito is unlikely to transmit the virus in the U.S. New knowledge about the Zika virus is being discovered on a daily basis but this research seems in line with what's known, which is that the main vector is the Aedes mosquito.The virus has been spreading across the Americas since last year. The World Health Organization declared a public health emergency of international concern in February. Since then, researchers have found the virus can cause a devastating birth defect, calledmicrocephaly, in babies born to mother's who were infected with the virus during pregnancy.http://www.cnn.com/2016/07/22/health/zika-culex-mosquito-brazil/ Link to comment Share on other sites More sharing options...
niman Posted July 24, 2016 Author Report Share Posted July 24, 2016 Health Officials Investigate Two Possible Cases Of Zika Virus Outbreak In FloridaJuly 24, 20167:48 AM ETHeard on Weekend Edition Sunday Federal officials investigate the first two cases of Zika that may have been contracted in southern Florida. NPR's Elise Hu and Michaeleen Doucleff discuss the new findings and their possible impact.Audio at:http://www.npr.org/2016/07/24/487237222/health-officials-investigate-two-possible-cases-of-zika-virus-outbreak-in-florid?utm_source=twitter.com&utm_campaign=health&utm_medium=social&utm_term=nprnews Link to comment Share on other sites More sharing options...
Recommended Posts
Please sign in to comment
You will be able to leave a comment after signing in
Sign In Now