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On 27 November 2015, the National IHR Focal Point of Paraguay notified PAHO/WHO of 6 laboratory-confirmed autochthonous cases of Zika virus infection in the city of Pedro Caballero, which is located in the northeast of the country and shares borders with Brazil.

The diagnoses were made by the national reference laboratory, the Central Public Health Laboratory of the Ministry of Health, through reverse transcription polymerase chain reaction (RT-PCR) technique. Cases were identified from clusters of febrile patients whose samples tested negative for dengue and chikungunya. They presented with fever, headache, myalgia, arthralgia, retroocular pain and nausea. Their age ranges from 14 to 45 years old, and half of them are female. Cases have been managed on an outpatient basis in different health care services of the health care network. Investigations are ongoing.

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