niman Posted July 13, 2015 Report Posted July 13, 2015 Thailand Department of Disease Control Ministry of Public Health release full N / ORF 8b MERS sequence which is identical to South Korea sequence.
niman Posted July 13, 2015 Author Report Posted July 13, 2015 LOCUS KT225476 1242 bp RNA linear VRL 07-JUL-2015DEFINITION Middle East respiratory syndrome coronavirus isolateMERS-CoV/THA/CU/17_06_2015 nucleocapsid and ORF8b genes, completecds.ACCESSION KT225476VERSION KT225476.1 GI:861032911KEYWORDS .SOURCE Middle East respiratory syndrome coronavirus (MERS-CoV)ORGANISM Middle East respiratory syndrome coronavirusViruses; ssRNA viruses; ssRNA positive-strand viruses, no DNAstage; Nidovirales; Coronaviridae; Coronavirinae; Betacoronavirus;unclassified Betacoronavirus.REFERENCE 1 (bases 1 to 1242)AUTHORS Plipat,T., Buathong,R., Siriarayapon,P., Wacharapluesadee,S.,Kaewpom,T., Prtcharat,S., Ponpinit,T., Jumpasri,J., Joyjinda,Y.,Rodpan,A., Jittmitraphap,A., Khongwichit,S., Smith,D.R. andHemachudha,T.TITLE First imported case of MERS-CoV infection in Thailand, June 2015JOURNAL UnpublishedREFERENCE 2 (bases 1 to 1242)AUTHORS Plipat,T., Buathong,R., Siriarayapon,P., Wacharapluesadee,S.,Kaewpom,T., Prtcharat,S., Ponpinit,T., Jumpasri,J., Joyjinda,Y.,Rodpan,A., Jittmitraphap,A., Khongwichit,S., Smith,D.R. andHemachudha,T.TITLE Direct SubmissionJOURNAL Submitted (30-JUN-2015) Department of Disease Control Ministry ofPublic Health, Bureau of Epidemiology, Tivanond Road, Muang,Nonthaburi 11000, ThailandCOMMENT ##Assembly-Data-START##Sequencing Technology :: Sanger dideoxy sequencing##Assembly-Data-END##FEATURES Location/Qualifierssource 1..1242/organism="Middle East respiratory syndrome coronavirus"/mol_type="genomic RNA"/isolate="MERS-CoV/THA/CU/17_06_2015"/isolation_source="sputum"/host="Homo sapiens"/db_xref="taxon:1335626"/country="Thailand"/collection_date="17-Jun-2015"CDS 1..1242/codon_start=1/product="nucleocapsid"/protein_id="AKO73399.1"/db_xref="GI:861032912"/translation="MASPAAPRAVSFADNNDITNTNLSRGRGRNPKPRAAPNNTVSWYTGLTQHGKVPLTFPPGQGVPLNANSTPAQNAGYWRRQDRKINTGNGIKQLAPRWYFYYTGTGPEAALPFRAVKDGIVWVHEDGATDAPSTFGTRNPNNDSAIVTQFAPGTKLPKNFHIEGTGGNSQSSSRASSVSRNSSRSSSQGSRSGNSTRGTSPGPSGIGAVGGDLLYLDLLNRLQALESGKVKQSQPKVITKKDAAAAKNKMRHKRTSTKSFNMVQAFGLRGPGDLQGNFGDLQLNKLGTEDPRWPQIAELAPTASAFMGMSQFKLTHQNNDDHGNPVYFLRYSGAIKLDPKNPNYNKWLELLEQNIDAYKTFPKKEKKQKAPKEESTDQMSEPPKEQRVQGSITQRTRTRPSVQPGPMIDVNTD"CDS 197..535/codon_start=1/product="ORF8b"/protein_id="AKO73400.1"/db_xref="GI:861032913"/translation="MPIPPLRKMLGIGGDRTEKLIPGMELSNWLPGGTSTTLELDPKQHSHSGLLRMASFGSMKMAPLMLLQLLGRGTLTMIQLLLHNSRPVLSFLKTSTLRGLEAIVNHLQEPLA"ORIGIN 1 atggcatccc ctgctgcacc tcgtgctgtt tcctttgccg ataacaatga tataacaaat61 acaaacctgt ctcgaggtag aggacgtaat ccaaaaccac gagctgcacc aaataacact121 gtctcttggt acactgggct tacccaacac gggaaagtcc ctcttacctt tccacctggg181 cagggtgtac ctcttaatgc caattccacc cctgcgcaaa atgctgggta ttggcggaga241 caggacagaa aaattaatac cgggaatgga attaagcaac tggctcccag gtggtacttc301 tactacactg gaactggacc cgaagcagca ctcccattcc gggctgttaa ggatggcatc361 gtttgggtcc atgaagatgg cgccactgat gctccttcaa cttttgggac gcggaaccct421 aacaatgatt cagctattgt tacacaattc gcgcccggta ctaagcttcc taaaaacttc481 cacattgagg ggactggagg caatagtcaa tcatcttcaa gagcctctag cgtaagcaga541 aactcttcca gatctagttc acaaggttca agatcaggaa actctacccg cggcacttct601 ccaggtccat ctggaatcgg agcagtagga ggtgatctac tttaccttga tcttctgaac661 agactacaag cccttgagtc tggcaaagta aagcaatcgc agccaaaagt aatcactaag721 aaagatgctg ctgctgctaa aaataagatg cgccacaagc gcacttccac caaaagtttc781 aacatggtgc aagcttttgg tcttcgcgga ccaggagacc tccagggaaa ctttggtgat841 cttcaattga ataaactcgg cactgaggac ccacgttggc cccaaattgc tgagcttgct901 cctacagcca gtgcttttat gggtatgtcg caatttaaac ttacccatca gaacaatgat961 gatcatggca accctgtgta cttccttcgg tacagtggag ccattaaact tgacccaaag1021 aatcccaact acaataagtg gttggagctt cttgagcaaa atattgatgc ctacaaaacc1081 ttccctaaga aggaaaagaa acaaaaggca ccaaaagaag aatcaacaga ccaaatgtct1141 gaacctccaa aggagcagcg tgtgcaaggt agcatcactc agcgcactcg cacccgtcca1201 agtgttcagc ctggtccaat gattgatgtt aacactgatt ag
niman Posted July 13, 2015 Author Report Posted July 13, 2015 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KT036374.1|Middle East respiratory syndrome coronavirus isolate ChinaGD01 variant 2, partial genome22412241100%0.0100%KT036374.1Select seq gb|KT036373.1|Middle East respiratory syndrome coronavirus isolate ChinaGD01 variant 1, partial genome22412241100%0.0100%KT036373.1Select seq gb|KT036372.1|Middle East respiratory syndrome coronavirus isolate ChinaGD01, partial genome22412241100%0.0100%KT036372.1Select seq gb|KT029139.1|Middle East respiratory syndrome coronavirus isolate MERS-CoV/KOR/KNIH/002_05_2015, complete genome22412241100%0.0100%KT029139.1Select seq gb|KT026453.1|Middle East respiratory syndrome coronavirus strain Hu/Riyadh_KSA_2959_2015, complete genome22412241100%0.0100%KT026453.1Select seq gb|KT006149.2|Middle East respiratory syndrome coronavirus strain ChinaGD01, complete genome22412241100%0.0100%KT006149.2Select seq gb|KJ713297.1|Middle East respiratory syndrome coronavirus isolate KSA-CAMEL-503, complete genome22412241100%0.0100%KJ713297.1Select seq gb|KJ156953.1|Middle East respiratory syndrome coronavirus isolate Riyadh_15f_2013 N protein and ORF8b genes, complete cds22412241100%0.0100%KJ156953.1Select seq gb|KJ156902.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_24g_2013 N protein and ORF8b genes, complete cds22412241100%0.0100%KJ156902.1Select seq gb|KJ156934.1|Middle East respiratory syndrome coronavirus isolate Riyadh_14_2013, complete genome22412241100%0.0100%KJ156934.1Select seq gb|KJ156866.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_25_2013, complete genome22412241100%0.0100%KJ156866.1Select seq gb|KF600652.1|Middle East respiratory syndrome coronavirus isolate Riyadh_2_2012, complete genome22412241100%0.0100%KF600652.1Select seq gb|KF600651.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_18_2013, complete genome22412241100%0.0100%KF600651.1Select seq gb|KF600647.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_17_2013, complete genome22412241100%0.0100%KF600647.1Select seq gb|KF600645.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_15_2013, complete genome22412241100%0.0100%KF600645.1Select seq gb|KF600644.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_16_2013, complete genome22412241100%0.0100%KF600644.1Select seq gb|KF600643.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_14b_2013, partial genome22412241100%0.0100%KF600643.1Select seq gb|KF600639.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_9e_2013, partial genome22412241100%0.0100%KF600639.1Select seq gb|KF600636.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_11c_2013, partial genome22412241100%0.0100%KF600636.1Select seq gb|KF600635.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_8d_2013, partial genome22412241100%0.0100%KF600635.1Select seq gb|KF600634.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_21_2013, complete genome22412241100%0.0100%KF600634.1Select seq gb|KF600628.1|Middle East respiratory syndrome coronavirus isolate Hafr-Al-Batin_1_2013, complete genome22412241100%0.0100%KF600628.1Select seq gb|KF600627.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_12_2013, complete genome22412241100%0.0100%KF600627.1Select seq gb|KF600621.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_22f_2013, partial genome22412241100%0.0100%KF600621.1Select seq gb|KF600620.1|Middle East respiratory syndrome coronavirus isolate Bisha_1_2012, complete genome22412241100%0.0100%KF600620.1Select seq gb|KF600612.1|Middle East respiratory syndrome coronavirus isolate Riyadh_1_2012, complete genome22412241100%0.0100%KF600612.1Select seq gb|KF192507.1|Middle East respiratory syndrome coronavirus, complete genome22412241100%0.0100%KF192507.1Select seq gb|KF186565.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_3_2013, complete genome22412241100%0.0100%KF186565.1Select seq gb|KF186567.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_1_2013, complete genome22412241100%0.0100%KF186567.1Select seq gb|KF186566.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_2_2013, complete genome22412241100%0.0100%KF186566.1Select seq gb|KF186564.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_4_2013, complete genome22412241100%0.0100%KF186564.1Select seq gb|KJ156862.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_23e_2013 N protein and ORF8b genes, complete cds22372237100%0.099%KJ156862.1Select seq gb|KT156560.1|Middle East respiratory syndrome coronavirus strain Hu/Oman_2285_2013, complete genome22352235100%0.099%KT156560.1Select seq gb|KT026456.1|Middle East respiratory syndrome coronavirus isolate Hu/Riyadh_KSA_4050_2015, complete genome22352235100%0.099%KT026456.1Select seq gb|KT026454.1|Middle East respiratory syndrome coronavirus strain Hu/Riyadh_KSA_4050_2015, complete genome22352235100%0.099%KT026454.1Select seq gb|KJ361503.1|Middle East respiratory syndrome coronavirus isolate Hu-France - FRA2_130569-2013_Isolate_Sanger, complete genome22352235100%0.099%KJ361503.1Select seq gb|KJ361502.1|Middle East respiratory syndrome coronavirus isolate Hu-France - FRA2_130569-2013_InSpu_Sanger, complete genome22352235100%0.099%KJ361502.1Select seq gb|KJ361501.1|Middle East respiratory syndrome coronavirus isolate Hu-France - FRA2_130569-2013_IS_HTS, complete genome22352235100%0.099%KJ361501.1Select seq gb|KJ361500.1|Middle East respiratory syndrome coronavirus isolate Hu-France (UAE) - FRA1_1627-2013_BAL_Sanger, complete genome22352235100%0.099%KJ361500.1Select seq gb|KP719933.1|Middle East respiratory syndrome coronavirus isolate Camel/UAE/D1209/2014, complete genome22352235100%0.099%KP719933.1Select seq gb|KP719932.1|Middle East respiratory syndrome coronavirus isolate Camel/UAE/D1243.12/2014, complete genome22352235100%0.099%KP719932.1Select seq gb|KP719931.1|Middle East respiratory syndrome coronavirus isolate Camel/UAE/D1339.2/2014, complete genome22352235100%0.099%KP719931.1Select seq gb|KP719930.1|Middle East respiratory syndrome coronavirus isolate Camel/UAE/D1164.14/2014, complete genome22352235100%0.099%KP719930.1Select seq gb|KP719929.1|Middle East respiratory syndrome coronavirus isolate Camel/UAE/D1164.11/2014, complete genome22352235100%0.099%KP719929.1Select seq gb|KP719928.1|Middle East respiratory syndrome coronavirus isolate Camel/UAE/D1164.10/2014, complete genome22352235100%0.099%KP719928.1Select seq gb|KP719927.1|Middle East respiratory syndrome coronavirus isolate Camel/UAE/D1164.9/2014, complete genome22352235100%0.099%KP719927.1Select seq gb|KM210278.1|Middle East respiratory syndrome coronavirus isolate England/3/2013, complete genome22352235100%0.099%KM210278.1Select seq gb|KM210277.1|Middle East respiratory syndrome coronavirus isolate England/4/2013, complete genome22352235100%0.099%KM210277.1Select seq gb|KP209313.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi_UAE_26_2014, complete genome22352235100%0.099%KP209313.1Select seq gb|KP209312.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi_UAE_9_2013, complete genome22352235100%0.099%KP209312.1Select seq gb|KP209309.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi_UAE_30_2014, complete genome22352235100%0.099%KP209309.1Select seq gb|KP209308.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi_UAE_16_2014, complete genome22352235100%0.099%KP209308.1Select seq gb|KP209307.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi_UAE_18_2014, complete genome22352235100%0.099%KP209307.1Select seq gb|KP209306.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi_UAE_8_2014, complete genome22352235100%0.099%KP209306.1Select seq gb|KM015348.1|Middle East respiratory syndrome coronavirus isolate England/2/2013, complete genome22352235100%0.099%KM015348.1Select seq gb|KF811035.1|Middle East respiratory syndrome coronavirus strain Tunisia-Qatar_2013 nucleocapsid protein gene, complete cds22352235100%0.099%KF811035.1Select seq gb|KJ713298.1|Middle East respiratory syndrome coronavirus isolate KSA-CAMEL-363, complete genome22352235100%0.099%KJ713298.1Select seq gb|KJ156927.1|Middle East respiratory syndrome coronavirus ORF4b, ORF5, E protein, M protein, N protein, and ORF8b genes, complete cds22352235100%0.099%KJ156927.1Select seq gb|KJ156865.1|Middle East respiratory syndrome coronavirus isolate Riyadh_17c_2013 ORF4a, ORF4b, ORF5, E protein, M protein, and N protein genes, complete cds; and ORF8b-like gene, complete sequence22352235100%0.099%KJ156865.1Select seq gb|KJ156909.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_28h_2013 N protein and ORF8b genes, complete cds22352235100%0.099%KJ156909.1Select seq gb|KJ156939.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_27b_2013 ORF1ab gene, partial cds; and S protein, ORF3, ORF4a, ORF4b, ORF5, E protein, M protein, N protein, and ORF8b genes, complete cds22352235100%0.099%KJ156939.1Select seq gb|KJ156876.1|Middle East respiratory syndrome coronavirus isolate Taif_2b_2013 ORF1ab gene, partial cds; and S protein, ORF3, ORF4a, ORF4b, ORF5, E protein, M protein, N protein, and ORF8b genes, complete cds22352235100%0.099%KJ156876.1Select seq gb|KJ156872.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_26c_2013 ORF1ab gene, partial cds; and S protein, ORF3, ORF4a, ORF4b, ORF5, E protein, M protein, N protein, and ORF8b genes, complete cds22352235100%0.099%KJ156872.1Select seq gb|KJ156932.1|Middle East respiratory syndrome coronavirus isolate Asir_2g_2013 M protein gene, partial cds; and N protein and ORF8b genes, complete cds22352235100%0.099%KJ156932.1Select seq gb|KJ156917.1|Middle East respiratory syndrome coronavirus isolate Madinah_1c_2013 M protein gene, partial cds; N protein gene, complete cds; and ORF8b-like gene, complete sequence22352235100%0.099%KJ156917.1Select seq gb|KJ156942.1|Middle East respiratory syndrome coronavirus isolate Riyadh_8b_2013, partial genome22352235100%0.099%KJ156942.1Select seq gb|KJ156916.1|Middle East respiratory syndrome coronavirus isolate Madinah_3b_2013, partial genome22352235100%0.099%KJ156916.1Select seq gb|KF961222.1|Middle East respiratory syndrome coronavirus isolate Qatar4, complete genome22352235100%0.099%KF961222.1Select seq gb|KF745068.1|Middle East respiratory syndrome coronavirus isolate FRA/UAE, complete genome22352235100%0.099%KF745068.1Select seq gb|KF600632.1|Middle East respiratory syndrome coronavirus isolate Al-Hasa_19_2013, complete genome22352235100%0.099%KF600632.1Select seq gb|KF600630.1|Middle East respiratory syndrome coronavirus isolate Buraidah_1_2013, complete genome22352235100%0.099%KF600630.1Select seq gb|KT156561.1|Middle East respiratory syndrome coronavirus strain Hu/Oman_2874_2013, complete genome22322232100%0.099%KT156561.1Select seq gb|KR011266.1|Middle East respiratory syndrome coronavirus strain Hu/Riyadh-KSA-2049/2015, complete genome22322232100%0.099%KR011266.1Select seq gb|KR011265.1|Middle East respiratory syndrome coronavirus strain Hu/Riyadh-KSA-2466/2015, complete genome22322232100%0.099%KR011265.1Select seq gb|KR011264.1|Middle East respiratory syndrome coronavirus strain Hu/Riyadh-KSA-2343/2015, complete genome22322232100%0.099%KR011264.1Select seq gb|KR011263.1|Middle East respiratory syndrome coronavirus strain Hu/Riyadh-KSA-2345/2015, complete genome22322232100%0.099%KR011263.1Select seq gb|KP209310.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi/Gayathi_UAE_2_2014, complete genome22322232100%0.099%KP209310.1Select seq gb|KJ614529.1|Human betacoronavirus 2c Jordan-N3/2012 isolate MG167, complete genome22322232100%0.099%KJ614529.1Select seq gb|KJ650297.1|Middle East respiratory syndrome coronavirus isolate KFU-HKU 1, complete genome22322232100%0.099%KJ650297.1Select seq gb|KJ650296.1|Middle East respiratory syndrome coronavirus isolate KFU-HKU 19Dam, complete genome22322232100%0.099%KJ650296.1Select seq gb|KJ650295.1|Middle East respiratory syndrome coronavirus isolate KFU-HKU 13, complete genome22322232100%0.099%KJ650295.1Select seq gb|KJ156901.1|Middle East respiratory syndrome coronavirus isolate Riyadh_12b_2013 ORF1ab gene, partial cds; and S protein, ORF3, ORF4a, ORF4b, ORF5, E protein, M protein, N protein, and ORF8b genes, complete cds22322232100%0.099%KJ156901.1Select seq gb|KJ156861.1|Middle East respiratory syndrome coronavirus isolate Hafr-Al-Batin_4d_2013 M protein gene, partial cds; and N protein and ORF8b genes, complete cds22322232100%0.099%KJ156861.1Select seq gb|KJ156906.1|Middle East respiratory syndrome coronavirus isolate Taif_3g_2013 M protein gene, partial cds; and N protein and ORF8b genes, complete cds22322232100%0.099%KJ156906.1Select seq gb|KJ156871.1|Middle East respiratory syndrome coronavirus isolate Taif_4b_2013 M protein gene, partial cds; and N protein and ORF8b genes, complete cds22322232100%0.099%KJ156871.1Select seq gb|KJ156952.1|Middle East respiratory syndrome coronavirus isolate Riyadh_4_2013, complete genome22322232100%0.099%KJ156952.1Select seq gb|KJ156949.1|Middle East respiratory syndrome coronavirus isolate Taif_1_2013, complete genome22322232100%0.099%KJ156949.1Select seq gb|KJ156910.1|Middle East respiratory syndrome coronavirus isolate Hafr-Al-Batin_2_2013, complete genome22322232100%0.099%KJ156910.1Select seq gb|KJ156881.1|Middle East respiratory syndrome coronavirus isolate Wadi-Ad-Dawasir_1_2013, complete genome22322232100%0.099%KJ156881.1Select seq gb|KJ156874.1|Middle East respiratory syndrome coronavirus isolate Hafr-Al-Batin_6_2013, complete genome22322232100%0.099%KJ156874.1Select seq gb|KF961221.1|Middle East respiratory syndrome coronavirus isolate Qatar3, complete genome22322232100%0.099%KF961221.1Select seq gb|KC776174.1|Human betacoronavirus 2c Jordan-N3/2012, complete genome22322232100%0.099%KC776174.1Select seq gb|KP236093.1|Middle East respiratory syndrome coronavirus strain Abu Dhabi/Oman_UAE_7_2013 N protein (N) gene, complete cds22282228100%0.099%KP236093.1Select seq gb|KM027262.1|Middle East respiratory syndrome coronavirus isolate Riyadh_2014KSA_683/KSA/2014, complete genome22262226100%0.099%KM027262.1Select seq gb|KJ813439.1|Middle East respiratory syndrome coronavirus strain Indiana/USA-1_Saudi Arabia_2014, complete genome22262226100%0.099%KJ813439.1Select seq gb|KJ650098.1|Middle East respiratory syndrome coronavirus isolate Camel/Qatar_2_2014, complete genome22262226100%0.099%KJ650098.1Select seq gb|KJ713299.1|Middle East respiratory syndrome coronavirus isolate KSA-CAMEL-376, complete genome22262226100%0.099%KJ713299.1Select seq gb|KJ713296.1|Middle East respiratory syndrome coronavirus isolate KSA-CAMEL-378, complete genome22262226100%0.099%KJ713296.1Select seq gb|KJ156883.1|Middle East respiratory syndrome coronavirus isolate Asir_1d_2013 N protein and ORF8b genes, complete cds22262226100%0.099%KJ156883.1Select seq gb|KJ156944.1|Middle East respiratory syndrome coronavirus isolate Riyadh_5_2013, complete genome22262226100%0.099%KJ156944.1 Alignments
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