niman Posted March 24, 2016 Report Share Posted March 24, 2016 Isolation of infective Zika virus from urine and saliva of patients in BrazilMyrna C Bonaldo, Ieda P Ribeiro, Noemia S. Lima, Alexandre A. C. Santos,Lidiane S.R. Menezes, Stephanie O.D. Cruz, Iasmim S. Mello, Nathália D. Furtado,Elaine E. Moura, Luana Damasceno, Keli A.B. Silva, Marcia G. Castro, Alexandra L. Gerber, Luiz G.P. Almeida, Ricardo Lourenço-de-Oliveira, Ana Tereza R.Vasconcelos, Patrícia Brasildoi: http://dx.doi.org/10.1101/045443 http://biorxiv.org/content/early/2016/03/24/045443 Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 AUTHOR INFORMATIONMyrna C Bonaldo1 (mbonaldo{at}ioc.fiocruz.br) (http://www.ioc.fiocruz.br),Ieda P Ribeiro1 (ieda.ribeiro{at}ioc.fiocruz.br) (http://www.ioc.fiocruz.br),Noemia S. Lima1 (noemiasl{at}gmail.com) (www.ioc.fiocruz.br), Alexandre A. C. Santos1 (xandeacs{at}ioc.fiocruz.br) (http://www.ioc.fiocruz.br), Lidiane S.R. Menezes1 (lidiane.raphael{at}ioc.fiocruz.br) (http://www.ioc.fiocruz.br),Stephanie O.D. Cruz1 (stephanieodc{at}gmail.com) (http://www.ioc.fiocruz.br),Iasmim S. Mello1 (mimmello123{at}hotmail.com) (http://www.ioc.fiocruz.br),Nathália D. Furtado1 (nathyfur{at}gmail.com) (http://www.ioc.fiocruz.br),Elaine E. Moura1 (evangelista.eem{at}gmail.com) (http://www.ioc.fiocruz.br),Luana Damasceno2 (luanasdamasceno{at}hotmail.com)(http://www.ini.fiocruz.br), Keli A.B. Silva1 (keli.antunes{at}ioc.fiocruz.br)(http://www.ioc.fiocruz.br), Marcia G. Castro1 (mcastro{at}ioc.fiocruz.br)(http://www.ioc.fiocruz.br), Alexandra L. Gerber3 (alegerber{at}lncc.br)(http://www.lncc.br), Luiz G.P. Almeida3 (lgonzaga{at}lncc.br)(http://www.lncc.br), Ricardo Lourenço-de-Oliveira1(lourenco{at}ioc.fiocruz.br) (http://www.ioc.fiocruz.br), Ana Tereza R. Vasconcelos3 (atrv{at}lncc.br) (http://www.lncc.br) and Patrícia Brasil2(patricia.brasil{at}ini.fiocruz.br) (http://www.ini.fiocruz.br)1 Instituto Oswaldo Cruz, Fiocruz;2 Instituto Nacional de Infectologia Evandro Chagas, Fiocruz;3 Laboratório Nacional de Computação Científica* Corresponding author; email: mbonaldo{at}ioc.fiocruz.br Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 AbstractZika virus (ZIKV) is an emergent threat provoking a worldwide explosive outbreak. Since January 2015, 41 countries reported autochthonous cases. In Brazil, an increase in Guillain-Barré syndrome and microcephaly cases was linked to ZIKV infections. A recent report describing low experimental transmission efficiency of its main putative vector, Ae. aegypti, in conjunction with apparent sexual transmission notifications prompted the investigation of other potential sources of viral dissemination. Urine and saliva have been previously established as useful tools in ZIKV diagnosis. However, no evidence regarding the infectivity of ZIKV particles present in saliva and urine has been obtained yet. Nine urine and five saliva samples from nine patients from Rio de Janeiro presenting rash and other typical Zika acute phase symptoms were inoculated in Vero cell culture and submitted to specific ZIKV RNA detection and quantification through, respectively, NAT-Zika, RT-PCR and RT-qPCR. Two ZIKV isolates were achieved, one from urine and one from saliva specimens. ZIKV nucleic acid was identified by all methods in four patients. Whenever both urine and saliva samples were available from the same patient, urine viral loads were higher, corroborating the general sense that it is a better source for ZIKV molecular diagnostic. In spite of this, from the two isolated strains, each from one patient, only one derived from urine, suggesting that other factors, like the acidic nature of this fluid, might interfere with virion infectivity. The complete genome of both ZIKV isolates was obtained. Phylogenetic analysis revealed similarity with strains previously isolated during the South America outbreak. The detection of infectious ZIKV particles in urine and saliva of patients during the acute phase may represent a critical factor in the spread of virus. The epidemiological relevance of this finding, regarding the contribution of alternative non vectorial ZIKV transmission routes, needs further investigation. Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 Link to comment Share on other sites More sharing options...
niman Posted March 24, 2016 Author Report Share Posted March 24, 2016 S1 Table Clinical symptomsPatient1234Onset date01/11/1601/24/1601/22/1601/31/16Days after symptoms onset *3251Days after rash onset *<1**111Rash duration5 days6 days2 days2 daysRash typeMacularMacular and maculo-papularMaculo-papularMaculo-papularLow grade fever(duration)-+ (2 days)+ (4 days)-Headache--+-Retro-orbital pain--+-Photophobia--NI-Fatigue/ malaise-++-Myalgia-++-Arthralgia(region)+ (wrist and elbows)-+ (large and small joints)-Arthritis----Anorexia--+-Nausea/ vomiting--+-Diarrhea----Abdominal pain----Dysuria----Bleeding/ petechia----Dizziness/ light headedness----Pruritus+-++Paresthesias+---Conjunctivitis----Edema(local/site)+ (hands)---Lymphadenopathy(local/ site)----EnanthemNI-NI-Respiratory symptoms----Jaundice----Seizures----* Regarding the date of sample collection; **the collection date was in the first day of rash manifestation S2 TableClinical symptomsPatient56789Onset date01/26/1601/26/1601/31/1601/31/1601/28/16Days after sypmtons onset *23125Days after rash onset *2<1122Rash duration 20 days 4 days5 days6 days3 daysRash typeMacularMaculo-papularMaculo-papularMacular and maculo-papularMaculo-papularLow grade fever(duration)-+ (1 day)-+ (2 days)-Headache+--++Retro-orbital pain+--++Photophobia+---+Fatigue/ malaise++-+-Myalgia++--+Arthralgia(region)-+ (wrists, ankles, knees, hands)-+ (large and small joints)-Arthritis-----Anorexia++---Nausea/ vomiting+----Diarrhea+----Abdominal pain-+---Dysuria-----Bleeding/ petechia ----Dizziness/ light headedness+----Pruritus+++++Paresthesias++---Conjunctivitis++--+Edema+ (feet and hands) +(hands)-+-Lymphadenopathy(local/ site)+(Cervical and auricular)---+(cervical)Enanthem-----Respiratory symptoms +--+-Jaundice-----Seizures-----* Regarding the date of sample collection; **the collection date was in the first day of rash manifestation S3 Table Social-demographic dataPatient1234GenderfemalefemalemalefemaleAge36302442Gestational age (weeks)1833NA21Family members illness--+-Partner illness--NI-Repellent spray use++NI+Previous DENV infection+-NI-Domicile in Rio de Janeiro StateDuque de CaxiasNova IguaçuRio de JaneiroDuque de CaxiasNA – not applicable; NI- not informed S4 Table Social demographic dataPatient56789Gender femalemalefemalefemalefemaleAge3068202722Gestational age (weeks)NANA172120Family members illness-+---Partner illness-----Repellent spray use+-+++Previous DENV infection++-+-Domicile in Rio de Janeiro StateRio de JaneiroRio de JaneiroRio de JaneiroDuque de CaxiasRio de JaneiroNA – not applicable; NI- not informed Link to comment Share on other sites More sharing options...
Recommended Posts
Please sign in to comment
You will be able to leave a comment after signing in
Sign In Now