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Full March 2016 Zika Sequence Rome ex-Brazil


niman

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LOCUS       KU991811               10643 bp    RNA     linear   VRL 29-MAR-2016
DEFINITION  Zika virus isolate Brazil/2016/INMI1 polyprotein gene, complete
            cds.
ACCESSION   KU991811
VERSION     KU991811.1  GI:1012128960
KEYWORDS    .
SOURCE      Zika virus
  ORGANISM  Zika virus
            Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA
            stage; Flaviviridae; Flavivirus.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Carletti,F., Meschi,S., Castilletti,C., Zaccaro,P., Ferraioli,V.,
            Quartu,S., Colavita,F., Corpolongo,A., Nicastri,E., Di Caro,A.,
            Ippolito,G. and Capobianchi,M.R.
  TITLE     Isolation of infectious Zika virus from sera in a traveller
            returning from Brazil to Italy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10643)
  AUTHORS   Carletti,F., Meschi,S., Castilletti,C., Zaccaro,P., Ferraioli,V.,
            Quartu,S., Colavita,F., Corpolongo,A., Nicastri,E., Di Caro,A.,
            Ippolito,G. and Capobianchi,M.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2016) Virology Laboratory, INMI L. Spallanzani,
            Via Portuense 292, Rome 00149, Italy
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Zika virus"
                     /mol_type="genomic RNA"
                     /isolate="Brazil/2016/INMI1"
                     /host="Homo sapiens"
                     /db_xref="taxon:64320"
                     /country="Italy: Rome"
                     /collection_date="06-Mar-2016"
     CDS             74..10345
                     /codon_start=1
                     /product="polyprotein"
                     /protein_id="AMS00611.1"
                     /db_xref="GI:1012128961"
                     /translation="MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPI
                     RMVLAILAFLRFTAIKPSLGLINRWGSVGKKEAMEIIKKFKKDLAAMLRIINARKEKK
                     RRGADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQI
                     MDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVT
                     LPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKV
                     IYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE
                     LVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG
                     NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET
                     DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF
                     HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE
                     MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG
                     TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG
                     EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG
                     AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC
                     SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR
                     MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGK
                     SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE
                     CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI
                     EESDLIIPKSLAGPLSHHNTREGYRTQMEGPWHSEELEIRFEECPGTKVHVEETCGTR
                     GPSLRSTTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKEPESNLVRSMVTA
                     GSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLA
                     ILMGATFAEMNTGGDVAHLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQT
                     AISALEGDLMVLINGFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGL
                     ATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSE
                     VLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKD
                     AEVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGAWY
                     VYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMW
                     HVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARN
                     IQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAIT
                     QGRREEETPVDCFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKTRLRTVILAP
                     TRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLY
                     IMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEV
                     PERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKT
                     KHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQR
                     RGRIGRNPNKPGDEYLYGGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADK
                     VAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIM
                     EDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTL
                     PGHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFV
                     LMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPEPEKQRSP
                     QDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIGFSMDIDLRPA
                     SAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPL
                     LMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRTAAGIMKNPVVDGIV
                     VTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSP
                     NKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQ
                     MSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKLRWLVERGYLQPYGK
                     VIDLGCGRGGWSYYAATIRKVQEVKGYTKGGPGHEEPVLVQSYGWNIVRLKSGVDVFH
                     MAAEPCDTLLCDIGESSSSPEVEEARTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMM
                     ETLERLQRRYGGGLVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRP
                     VKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG
                     SYEAPTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPD
                     PQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEKEWKTAV
                     EAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGA
                     RFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSRIPGGRMYADDTAGWD
                     TRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQ
                     DQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDR
                     LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHH
                     FNKLHLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRR
                     DLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKT
                     PVTKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST
                     QVRYLGEEGSTPGVL"
ORIGIN      
        1 tcgagtttga agcgaaagct agcaacagta tcaacaggtt ttattttgga tttggaaacg
       61 agagtttctg gtcatgaaaa acccaaaaaa gaaatccgga ggattccgga ttgtcaatat
      121 gctaaaacgc ggagtagccc gtgtgagccc ctttgggggc ttgaagaggc tgccagccgg
      181 acttctgctg ggtcatgggc ccatcaggat ggtcttggcg attctagcct ttttgagatt
      241 cacggcaatc aagccatcac tgggtctcat caatagatgg ggttcagtgg ggaaaaaaga
      301 ggctatggaa ataataaaga agttcaagaa agatctggct gccatgctga gaataatcaa
      361 tgctaggaag gagaagaaga gacgaggcgc agatactagt gtcggaattg ttggcctcct
      421 gctgaccaca gctatggcag cggaggtcac tagacgtggg agtgcatact atatgtactt
      481 ggacagaaac gatgctgggg aggccatatc ttttccaacc acattgggga tgaataagtg
      541 ttatatacag atcatggatc ttggacacat gtgtgatgcc accatgagct atgaatgccc
      601 tatgctggat gagggggtgg aaccagatga cgtcgattgt tggtgcaaca cgacgtcaac
      661 ttgggttgtg tacggaacct gccatcacaa aaaaggtgaa gcacggagat ctagaagagc
      721 tgtgacgctc ccctcccatt ccactaggaa gctgcaaacg cggtcgcaaa cctggttgga
      781 atcaagagaa tacacaaagc acttgattag agtcgaaaat tggatattca ggaaccctgg
      841 cttcgcgtta gcagcagctg ccatcgcttg gcttttggga agctcaacga gccaaaaagt
      901 catatacttg gtcatgatac tgctgattgc cccggcatac agcatcaggt gcataggagt
      961 cagcaatagg gactttgtgg aaggtatgtc aggtgggact tgggttgatg ttgtcttgga
     1021 acatgggggt tgtgtcaccg taatggcaca ggacaaaccg actgtcgaca tagagctggt
     1081 tacaacaaca gtcagcaaca tggcggaggt aagatcctac tgctatgagg catcaatatc
     1141 agacatggct tcggacagcc gctgcccaac acaaggtgaa gcctaccttg acaagcaatc
     1201 agacactcaa tatgtctgca aaagaacgtt agtggacaga ggctggggaa atggatgtgg
     1261 actttttggc aaagggagcc tggtgacatg cgctaagttt gcatgctcca agaaaatgac
     1321 cgggaagagc atccagccag agaatctgga gtaccggata atgctgtcag ttcatggctc
     1381 ccagcacagt gggatgatcg ttaatgacac aggacatgaa actgatgaga atagagcgaa
     1441 ggttgagata acgcccaatt caccaagagc cgaagccacc ctggggggtt ttggaagcct
     1501 aggacttgat tgtgaaccga ggacaggcct tgacttttca gatttgtatt acttgactat
     1561 gaataacaag cactggttgg ttcacaagga gtggttccac gacattccat taccttggca
     1621 cgctggggca gacaccggaa ctccacactg gaacaacaaa gaagcactgg tagagttcaa
     1681 ggacgcacat gccaaaaggc aaactgtcgt ggttctaggg agtcaagaag gagcagttca
     1741 cacggccctt gctggagctc tggaggctga gatggatggt gcaaagggaa ggctgtcctc
     1801 tggccacttg aaatgtcgcc tgaaaatgga taaacttaga ttgaagggcg tgtcatactc
     1861 cttgtgtacc gcagcgttca cattcaccaa gatcccggct gaaacactgc acgggacagt
     1921 cacagtggag gtacagtacg cagggacaga tggaccttgc aaggttccag ctcagatggc
     1981 ggtggacatg caaactctga ccccagttgg gaggttgata accgctaacc ccgtaatcac
     2041 tgaaagcact gagaactcta agatgatgct ggaacttgat ccaccatttg gggactctta
     2101 cattgtcata ggagtcgggg agaagaagat cacccaccac tggcacagga gtggcagcac
     2161 cattggaaaa gcatttgaag ccactgtgag aggtgccaag agaatggcag tcttgggaga
     2221 cacagcctgg gactttggat cagttggagg cgctctcaac tcattgggca agggcatcca
     2281 tcaaattttt ggagcagctt tcaaatcatt gtttggagga atgtcctggt tctcacaaat
     2341 tctcattgga acgttgctga tgtggttggg tctgaacaca aagaatggat ctatttccct
     2401 tatgtgcttg gccttagggg gagtgttgat cttcttatcc acagccgtct ctgctgatgt
     2461 ggggtgttcg gtggacttct caaagaagga gacgagatgt ggtacagggg tgttcgtcta
     2521 taacgacgtt gaagcctgga gggacaggta caagtaccat cctgactccc cccgtagatt
     2581 ggcagcagca gtcaagcaag cctgggaaga tggtatctgc gggatctcct ctgtttcaag
     2641 aatggaaaat atcatgtgga gatcagtaga aggggagctc aacgcaatcc tggaagagaa
     2701 tggagttcaa ctgacggtcg ttgtgggatc tgtaaaaaac cccatgtgga gaggtccaca
     2761 gagattgccc gtgcctgtga acgagctgcc ccacggctgg aaggcttggg ggaaatcgta
     2821 cttcgtcaga gcagcaaaga caaataacag ctttgtcgtg gatggtgaca cactgaagga
     2881 atgcccactc aaacatagag catggaacag ctttcttgtg gaggatcatg ggttcggggt
     2941 atttcacact agtgtctggc tcaaggttag agaagattat tcattagagt gtgatccagc
     3001 cgttattgga acagctgtta agggaaagga ggctgtacac agtgatctag gctactggat
     3061 tgagagtgag aagaatgaca catggaggct gaagagggcc catctgatcg agatgaaaac
     3121 atgtgaatgg ccaaagtccc acacattgtg gacagatgga atagaagaga gtgatctgat
     3181 catacccaag tctttagctg ggccactcag ccatcacaat accagagagg gctacaggac
     3241 ccaaatggaa gggccatggc acagtgaaga gcttgaaatt cggtttgagg aatgcccagg
     3301 cactaaggtc cacgtggagg aaacatgtgg aacaagagga ccatctctga gatcaaccac
     3361 tgcaagcgga agggtgatcg aggaatggtg ctgcagggag tgcacaatgc ccccactgtc
     3421 gttccgggct aaagatggct gttggtatgg aatggagata aggcccagga aagaaccaga
     3481 aagcaactta gtaaggtcaa tggtgactgc aggatcaact gatcacatgg atcacttctc
     3541 ccttggagtg cttgtgattc tgctcatggt gcaggaaggg ctgaagaaga gaatgaccac
     3601 aaagatcatc ataagcacat caatggcagt gctggtagct atgatcctgg gaggattttc
     3661 aatgagtgac ctggctaagc ttgcaatttt gatgggtgcc accttcgcgg aaatgaacac
     3721 tggaggagat gtagctcatc tggcgctgat agcggcattc aaagtcagac cagcgttgct
     3781 ggtatctttc atcttcagag ctaattggac accccgtgaa agcatgctgc tggccttggc
     3841 ctcgtgtctt ttgcaaactg cgatctccgc cttggaaggc gacctgatgg ttctcatcaa
     3901 tggttttgct ttggcctggt tggcaatacg agcgatggtt gttccacgca ctgacaacat
     3961 caccttggca atcctggctg ctctgacacc actggcccgg ggcacactgc ttgtggcgtg
     4021 gagagcaggc cttgctactt gcggggggtt tatgctcctc tctctgaagg gaaaaggcag
     4081 tgtgaagaag aacttaccat ttgtcatggc cctgggacta accgctgtga ggctggtcga
     4141 ccccatcaac gtggtgggac tgctgttgct cacaaggagt gggaagcgga gctggccccc
     4201 tagcgaagta ctcacagctg ttggcctgat atgcgcattg gctggagggt tcgccaaggc
     4261 agatatagag atggctgggc ccatggccgc ggtcggtctg ctaattgtca gttacgtggt
     4321 ctcaggaaag agtgtggaca tgtacattga aagagcaggt gacatcacat gggaaaaaga
     4381 tgcggaagtc actggaaaca gtccccggct cgatgtggcg ctagatgaga gtggtgattt
     4441 ctccctggtg gaggatgacg gtccccccat gagagagatc atactcaagg tggtcctgat
     4501 gaccatctgt ggcatgaacc caatagccat accctttgca gctggagcgt ggtacgtata
     4561 cgtgaagact ggaaaaagga gtggtgctct atgggatgtg cctgctccca aggaagtaaa
     4621 aaagggggag accacagatg gagtgtacag agtaatgact cgcagactgc taggttcaac
     4681 acaagttgga gtgggagtta tgcaagaggg ggtctttcac actatgtggc acgtcacaaa
     4741 aggatccgcg ctgagaagcg gtgaagggag acttgatcca tactggggag atgtcaagca
     4801 ggatctggtg tcatactgtg gtccgtggaa gctagatgcc gcctgggacg ggcacagcga
     4861 ggtgcagctc ttggccgtgc cccccggaga gagagcgagg aacatccaga ctctgcccgg
     4921 aatatttaag acaaaggatg gggacattgg agcggttgcg ctggattacc cagcaggaac
     4981 ttcaggatct ccaatcctag acaagtgtgg gagagtgata ggactttatg gcaatggggt
     5041 cgtgatcaaa aatgggagtt atgttagtgc catcacccaa gggaggaggg aggaagagac
     5101 tcctgttgat tgcttcgagc cttcgatgct gaagaagaag cagctaactg tcttagactt
     5161 gcatcctgga gctgggaaaa ccaggagagt tcttcctgaa atagtccgtg aagccataaa
     5221 aacaagactc cgtactgtga tcttagctcc aaccagggtt gtcgctgctg aaatggagga
     5281 agcccttaga gggcttccag tgcgttatat gacaacagca gtcaatgtca cccactctgg
     5341 aacagaaatc gtcgacttaa tgtgccatgc caccttcact tcacgtctac tacagccaat
     5401 cagagtcccc aactataatc tgtatattat ggatgaggcc cacttcacag atccctcaag
     5461 tatagcagca agaggataca tttcaacaag ggttgagatg ggcgaggcgg ctgccatctt
     5521 catgaccgcc acgccaccag gaacccgtga cgcatttccg gactccaact caccaattat
     5581 ggacaccgaa gtggaagtcc cagagagagc ctggagctca ggctttgatt gggtgacgga
     5641 tcattctgga aaaacagttt ggtttgttcc aagcgtgagg aacggcaatg agatcgcagc
     5701 ttgtctgaca aaggctggaa aacgggtcat acagctcagc agaaagactt ttgagacaga
     5761 gttccagaaa acaaaacatc aagagtggga ctttgtcgtg acaactgaca tttcagagat
     5821 gggcgccaac tttaaagctg accgtgtcat agattccagg agatgcctaa agccggtcat
     5881 acttgatggc gagagagtca ttctggctgg acccatgcct gtcacacatg ccagcgctgc
     5941 ccagaggagg gggcgcatag gcaggaatcc caacaaacct ggagatgagt atctgtatgg
     6001 aggtgggtgc gcagagactg acgaagacca tgcacactgg cttgaagcaa gaatgctcct
     6061 tgacaatatt tacctccaag atggcctcat agcctcgctc tatcgacctg aggccgacaa
     6121 agtagcagcc attgagggag agttcaagct taggacggag caaaggaaga cctttgtgga
     6181 actcatgaaa agaggagatc ttcctgtttg gctggcctat caggttgcat ctgccggaat
     6241 aacctacaca gatagaagat ggtgctttga tggcacgacc aacaacacca taatggaaga
     6301 cagtgtgccg gcagaggtgt ggaccagaca cggagagaaa agagtgctca aaccgaggtg
     6361 gatggacgcc agagtttgtt cagatcatgc ggccctgaag tcattcaagg agtttgccgc
     6421 tgggaaaaga ggagcggctt ttggagtgat ggaagccctg ggaacactgc caggacacat
     6481 gacagagaga ttccaggaag ccattgacaa cctcgctgtg ctcatgcggg cagagactgg
     6541 aagcaggcct tacaaagccg cggcggccca attgccggag accctagaga ccattatgct
     6601 tttggggttg ctgggaacag tctcgctggg aatctttttc gtcttgatga ggaacaaggg
     6661 catagggaag atgggctttg gaatggtgac tcttggggcc agcgcatggc tcatgtggct
     6721 ctcggaaatt gagccagcca gaattgcatg tgtcctcatt gttgtgttcc tattgctggt
     6781 ggtgctcata cctgagccag aaaagcaaag atctccccag gacaaccaaa tggcaatcat
     6841 catcatggta gcagtaggtc ttctgggctt gattaccgcc aatgaactcg gatggttgga
     6901 gagaacaaag agtgacctaa gccatctaat gggaaggaga gaggaggggg caaccatagg
     6961 attctcaatg gacattgacc tgcggccagc ctcagcttgg gccatctatg ctgccttgac
     7021 aactttcatt accccagccg tccaacatgc agtgaccact tcatacaaca actactcctt
     7081 aatggcgatg gccacgcaag ctggagtgtt gtttggtatg ggcaaaggga tgccattcta
     7141 cgcatgggac tttggagtcc cgctgctaat gataggttgc tactcacaat taacacccct
     7201 gaccctaata gtggccatca ttttgctcgt ggcgcactac atgtacttga tcccagggct
     7261 gcaggcagca gctgcgcgtg ctgcccagaa gagaacggca gctggcatca tgaagaaccc
     7321 tgttgtggat ggaatagtgg tgactgacat tgacacaatg acaattgacc cccaagtgga
     7381 gaaaaagatg ggacaggtgc tactcatagc agtagccgtc tccagcgcca tactgtcgcg
     7441 gaccgcctgg gggtgggggg aggctggggc cctgatcaca gccgcaactt ccactttgtg
     7501 ggaaggctct ccgaacaagt actggaactc ctctacagcc acttcactgt gtaacatttt
     7561 taggggaagt tacttggctg gagcttctct aatctacaca gtaacaagaa acgctggctt
     7621 ggtcaagaga cgtgggggtg gaacaggaga gaccctggga gagaaatgga aggcccgctt
     7681 gaaccagatg tcggccctgg agttctactc ctacaaaaag tcaggcatca ccgaggtgtg
     7741 cagagaagag gcccgccgcg ccctcaagga cggtgtggca acgggaggcc atgctgtgtc
     7801 ccgaggaagt gcaaagctga gatggttggt ggagcgggga tacctgcagc cctatggaaa
     7861 ggtcattgat cttggatgtg gcagaggggg ctggagttac tacgccgcca ccatccgcaa
     7921 agttcaagaa gtgaaaggat acacaaaagg aggccctggt catgaagaac ccgtgttggt
     7981 gcaaagctat gggtggaaca tagtccgtct taagagtggg gtggacgtct ttcatatggc
     8041 ggctgagccg tgtgacacgt tgctgtgtga cataggtgag tcatcatcta gtcctgaagt
     8101 ggaagaagca cggacgctca gagtcctctc catggtgggg gattggcttg aaaaaagacc
     8161 aggagccttt tgtataaaag tgttgtgccc atacaccagc actatgatgg aaaccctgga
     8221 gcgactgcag cgtaggtatg ggggaggact ggtcagagtg ccactctccc gcaactctac
     8281 acatgagatg tactgggtct ctggagcgaa aagcaacacc ataaaaagtg tgtccaccac
     8341 gagccagctc ctcttggggc gcatggacgg gcctaggagg ccagtgaaat atgaggagga
     8401 tgtgaatctc ggctctggca cgcgggctgt ggtaagctgc gctgaagctc ccaatatgaa
     8461 gatcattggt aaccgcattg aaaggatccg cagtgagcac gcggaaacgt ggttctttga
     8521 cgagaaccac ccatatagga catgggctta ccatggaagc tatgaggccc ccacacaagg
     8581 gtcagcgtcc tctctaataa acggggttgt caggctcctg tcaaaaccct gggatgtggt
     8641 gactggagtc acaggaatag ccatgaccga caccacaccg tatggtcagc aaagagtttt
     8701 caaggaaaaa gtggacacta gggtgccaga cccccaagaa ggcactcgtc aggttatgag
     8761 catggtctct tcctggttgt ggaaagagct aggcaaacac aaacggccac gagtctgtac
     8821 caaagaagag ttcatcaaca aggttcgtag caatgcagca ttaggggcaa tatttgaaga
     8881 ggaaaaagag tggaagactg cagtggaagc tgtgaacgat ccaaggttct gggctctagt
     8941 ggacaaggaa agagagcacc acctgagagg agagtgccag agttgtgtgt acaacatgat
     9001 gggaaaaaga gaaaagaaac aaggggaatt tggaaaggcc aagggcagcc gcgccatctg
     9061 gtatatgtgg ctaggggcta gatttctaga gttcgaagcc cttggattct tgaacgagga
     9121 tcactggatg gggagagaga actcaggagg tggtgttgaa gggctgggat tacaaagact
     9181 cggatatgtc ctagaagaga tgagtcgcat accaggagga aggatgtatg cagatgacac
     9241 tgctggctgg gatacccgca tcagcaggtt tgatctggag aatgaagctc taatcaccaa
     9301 ccaaatggag aaagggcaca gggccttggc attggccata atcaagtaca cataccaaaa
     9361 caaagtggta aaggtcctta gaccagctga aaaagggaaa acagttatgg acattatttc
     9421 gagacaagac caaaggggga gcggacaagt tgtcacttac gctcttaaca catttaccaa
     9481 cctagtggtg caactcattc ggaatatgga ggctgaggaa gttctagaga tgcaagactt
     9541 gtggctgctg cggaggtcag agaaagtgac caactggttg cagagcaacg gatgggatag
     9601 gctcaaacga atggcagtca gtggagatga ttgcgttgtg aagccaattg atgataggtt
     9661 tgcacatgcc ctcaggttct tgaatgatat gggaaaagtt aggaaggaca cacaagagtg
     9721 gaaaccctca actggatggg acaactggga agaagttccg ttttgctccc accacttcaa
     9781 caagctccat ctcaaggacg ggaggtccat tgtggttccc tgccgccacc aagatgaact
     9841 gattggccgg gcccgcgtct ctccaggggc gggatggagc atccgggaga ctgcttgcct
     9901 agcaaaatca tatgcgcaga tgtggcagct cctttatttc cacagaaggg acctccgact
     9961 gatggccaat gccatttgtt catctgtgcc agttgactgg gttccaactg ggagaactac
    10021 ctggtcaatc catggaaagg gagaatggat gaccactgaa gacatgcttg tggtgtggaa
    10081 cagagtgtgg attgaggaga acgaccacat ggaagacaag accccagtta cgaaatggac
    10141 agacattccc tatttgggaa aaagggaaga cttgtggtgt ggatctctca tagggcacag
    10201 accgcgcacc acctgggctg agaacattaa aaacacagtc aacatggtgc gcaggatcat
    10261 aggtgatgaa gaaaagtaca tggactacct atccacccaa gttcgctact tgggtgaaga
    10321 agggtctaca cctggagtgc tgtaagcacc aatcttaatg ttgtcaggcc tgctagtcag
    10381 ccacagcttg gggaaagctg tgcagcctgt gaccctccca ggagaagctg ggaaaccaag
    10441 cctatagtca ggccgagaac gccatggcac ggaagaagcc atgctgcctg tgagcccctc
    10501 agaggacact gagtcaaaaa accccacgcg cttggaggcg caggatggga aaagaaggtg
    10561 gcgaccttcc ccacccttca atctggggcc tgaactggag atcagctgtg gatctccaga
    10621 agagggacta gtggttagag gag
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