niman

https://www.health.pa.gov/topics/disease/coronavirus/Pages/Cases.aspx

Filtered to: Louisiana 48,634 Cases Reported 2,950 Deaths Reported Tests Performed 28,415 by State Lab Commercial Tests 516,806 Performed Statewide Indicator Presumed Recovered* 37,017 as of 6/14/2020 Reported COVID-19 Patients in Hospitals 585 83 of those on ventilators http://ldh.la.gov/coronavirus/

https://www.coronavirus.kdheks.gov/160/COVID-19-in-Kansas

https://experience.arcgis.com/experience/96dd742462124fa0b38ddedb9b25e429

By LOS ANGELES TIMES STAFF UPDATED JUNE 17, 9:39 A.M. PACIFIC 159,231 confirmed cases +48 today +3,444 yesterday 5,203 deaths +1 today +87 yesterday The coronavirus pandemic has spread rapidly across California. Experts say the true number of people infected is unknown and likely much higher than official tallies. To better understand the spread of the virus, The Times is conducting an independent, continual survey of dozens of local health agencies across the state. So far today, three of the 61 agencies we’re monitoring have reported new numbers. What we know Tallies continue to climb. Over the past week, the state has averaged 3,161 new cases and 64.7 new deaths per day. SoCal remains a hot spot. Los Angeles County has recorded 43% of new cases in the last two weeks, while home to only a quarter of the state's population. Hospitals are holding up. The number of patients remains steady, a goal of the stay-at-home policies. Testing is increasing. Numbers are rising. The state is hovering near its goal of 60,000 tests per day. The highest toll is among seniors. Roughly 78% of the dead were 65 or older. At least 2,613 were living at a nursing home. The state is gradually reopening. Most counties continue to ease restrictions. California's totals still sit far below New York, where more than 30,900 people have died. California counties Alameda Contra Costa Fresno Imperial Kern Kings Los Angeles Monterey Orange Riverside Sacramento San Bernardino San Diego San Francisco San Joaquin San Mateo Santa Barbara Santa Clara Sonoma Tulare Ventura Other trackers Beach closures Housing homeless people Reopenings by county The lives lost More coverage Symptoms How it spreads Get our newsletter Jump to a section Trends Maps Hospitals Tests Demographics Nursing homes Reopenings State rankings The latest trends The number of cases in California is now on pace to double every 38.1 days, a number used to measure how quickly the virus is spreading. Coronavirus can infect people so rapidly that government officials have issued shutdown orders aimed at slowing the growth of new cases and flattening this line. CasesDeaths Cumulative cases Feb.MarchAprilMayJune020,00040,00060,00080,000100,000120,000140,000160,000 Times survey of county and local health departments Local governments announce new cases and deaths each day, though bottlenecks in testing and reporting lags can introduce delays. For instance, some agencies do not report new totals on weekends, leading to lower numbers on those days. New cases by day Feb.MarchAprilMayJune01,0002,0003,0004,0007-dayaverage7-dayaverage Deaths by day Feb.MarchAprilMayJune0501007-dayaverage7-dayaverage The lines above are seven-day averages. They offer a more stable view of the trend than daily totals. That's why experts wait for lines like these to flatten before they say conditions are improving. The rate varies from county to county, but most areas are still reporting new cases every day. The chart below is adjusted to show how quickly new cases are being confirmed in each county. A good sign is when a line flattens, which indicates that transmission is slowing in that area. Cumulative cases by county Current doubling time5 days7142130 15 dayssince 10th case30456075901020501002005001,0002,0005,00010,00020,00040,00060,000Doublingevery dayDoublingevery dayEvery2 daysEvery2 daysEvery3 daysEvery3 daysEvery weekEvery weekEverymonthEverymonthGlennGlennSutterSutterImperialImperialMontereyMontereyNapaNapaCalaverasCalaverasFresnoFresnoRiversideRiversideMendocinoMendocinoAmadorAmadorContra CostaContra CostaSolanoSolanoLos AngelesLos AngelesYubaYubaSanta ClaraSanta ClaraMonoMonoTehamaTehamaPlacerPlacerSan BenitoSan BenitoMercedMercedSan BernardinoSan BernardinoVenturaVenturaNevadaNevadaKingsKingsInyoInyoSanta CruzSanta CruzMariposaMariposa This chart tracks cumulative cases after each county confirmed its 10th case. Case counts are plotted on a logarithmic scale, which makes it easier to see when cases level off. Doubling rate is the estimate of how long it would take the county to double its number of cases, given the trend in the last week. Compare the slope of a county's curve to the slope of the guide lines to estimate its doubling rate at any point. Help us track the coronavirus by subscribing Your support makes our reporting possible. Get unlimited digital access today. Already a subscriber? Your contributions help us maintain this page. Thank you. Cases by county and city While initial outbreaks were centered in the San Francisco Bay Area, most new cases and deaths are now concentrated in Southern California. Officials are watching the latest figures as they weigh when and how to reopen. One metric is whether counties have kept new cases over the last 14 days to less than 25 per 100,000 residents. Currently, 25 of 58 counties pass the test. Metric CasesDeaths Time frame Last 14 daysCumulative Confirmed cases02907002,0502,4203,53017,950 Hover for more information. Los AngelesLos AngelesSan DiegoSan DiegoSacramentoSacramentoSan FranciscoSan FranciscoReddingRedding In the last 14 days Cases Per 100k Status Today Deaths Per 100k Today Los Angeles » 17,950 177.8 Failing – 513 5.1 – Riverside » 3,539 148.5 Failing – 53 2.2 – Imperial » 2,591 1,437.7 Failing – 23 12.8 – Orange » 2,425 76.6 Failing – 83 2.6 – San Bernardino » 2,295 107.5 Failing – 20 0.9 – San Diego » 2,056 62.2 Failing – 47 1.4 – Kern » 1,076 121.8 Failing – 15 1.7 – Alameda » 966 58.8 Failing – 15 0.9 – Fresno » 874 89.4 Failing – 20 2 – Tulare » 794 172.4 Failing – 9 2 – San Joaquin » 767 104.8 Failing – 12 1.6 – Kings » 702 467.8 Failing – 3 2 – Contra Costa » 520 45.9 Failing – 12 1.1 – Stanislaus 500 92.7 Failing – 5 0.9 – Ventura » 491 57.9 Failing – 7 0.8 – Monterey » 444 102.5 Failing – 2 0.5 – San Mateo » 437 57.1 Failing +28 15 2 – Santa Clara » 435 22.6 Passing – 8 0.4 – Santa Barbara » 418 94.2 Failing – 8 1.8 – Sacramento » 386 25.6 Failing – 5 0.3 – San Francisco » 384 44.1 Failing +11 3 0.3 +1 Marin 299 114.9 Failing – 1 0.4 – Sonoma » 186 37.1 Failing – – – – Placer 183 48.1 Failing – – – – Merced 162 60.2 Failing – – – – Solano 140 31.9 Failing – 1 0.2 – Madera 133 85.8 Failing – 1 0.6 – Napa 86 61.2 Failing – – – – San Luis Obispo 78 27.7 Failing – – – – Yolo 52 24.2 Passing – – – – San Benito 46 77.4 Failing – – – – Santa Cruz 43 15.7 Passing +9 – – – Sutter 43 44.9 Failing – 1 1 – Butte 37 16.3 Passing – – – – El Dorado 22 11.8 Passing – – – – Tehama 22 34.7 Failing – – – – Glenn 17 60.9 Failing – – – – Mendocino 17 19.4 Passing – – – – Calaveras 11 24.3 Passing – – – – Shasta 11 6.1 Passing – – – – Siskiyou 11 25.3 Failing – – – – Nevada 9 9.1 Passing – – – – Yuba 6 7.9 Passing – – – – Tuolumne 5 9.3 Passing – – – – Humboldt 4 2.9 Passing – – – – Inyo 4 22.1 Passing – – – – Lake 4 6.2 Passing – – – – Lassen 4 12.8 Passing – – – – Amador 2 5.3 Passing – – – – Del Norte 1 3.6 Passing – – – – Mariposa 1 5.7 Passing – – – – Plumas 1 5.3 Passing – – – – Trinity 1 7.8 Passing – – – – Alpine – – Passing – – – – Colusa – – Passing – – – – Modoc – – Passing – – – – Mono – – Passing – – – – Sierra – – Passing – – – – Show less Residents of cities, neighborhoods and regions all across the state have contracted the coronavirus. Here are the latest tallies for 906 places as released by county health departments. Confirmed cases 10 100 500 1,000 2,000 Counties that do not report cases by locality © Mapbox © OpenStreetMap Improve this map The following counties currently do not report cases by locality: Alpine, Colusa, Del Norte, Glenn, Inyo, Lake, Lassen, Madera, Mariposa, Modoc, San Benito, Shasta, Sierra, Siskiyou, Sutter, Tehama, Trinity, Tuolumne and Yuba Filter by countyAlameda Amador Butte Calaveras Contra Costa El Dorado Fresno Humboldt Imperial Kern Kings Los Angeles Marin Mendocino Merced Mono Monterey Napa Nevada Orange Placer Plumas Riverside Sacramento San Bernardino San Diego San Francisco San Joaquin San Luis Obispo San Mateo Santa Barbara Santa Clara Santa Cruz Solano Sonoma Stanislaus Tulare Ventura Yolo City/community Confirmed cases East Los Angeles 1,741 Castaic 1,721 Boyle Heights 1,207 Glendale 1,170 South Gate 1,105 Downey 1,068 Westlake 1,060 Pasadena 1,058 Sylmar 1,047 Panorama City 1,039 San Pedro 1,034 Pacoima 1,023 Compton 980 El Monte 969 Van Nuys 966 Palmdale 959 Lynwood 958 Wholesale District 954 Vernon Central 953 Unincorporated - Florence-Firestone 949 North Hollywood 925 Santa Clarita 907 Pomona 874 Huntington Park 799 Lancaster 783 Reseda 776 Inglewood 770 Norwalk 754 Pico-Union 748 Pico Rivera 713 Melrose 696 West Vernon 684 Central 670 Florence-Firestone 653 Canoga Park 628 South Park 618 North Hills 614 Montebello 610 Bellflower 575 Paramount 565 West Covina 562 Hawthorne 552 Vermont Vista 548 Carson 545 Baldwin Park 535 90805: Long Beach 500 Bell 497 Watts 489 Bell Gardens 469 Whittier 467 Torrance 457 Harvard Park 456 Maywood 453 Burbank 447 Granada Hills 447 90813: Long Beach 442 Northridge 439 Century Palms/Cove 435 Temple-Beaudry 424 Wilmington 408 Sun Valley 402 Lincoln Heights 386 Willowbrook 378 Koreatown 376 Winnetka 372 Gardena 363 Cudahy 357 Exposition Park 352 Arleta 351 Highland Park 345 Hollywood 342 Santa Monica 340 Wilshire Center 332 Athens-Westmont 331 90806: Long Beach 328 West Adams 320 Alhambra 292 Tarzana 291 Lakewood 286 Sherman Oaks 286 El Sereno 284 Azusa 283 University Park 276 Covina 263 South Whittier 263 Lake Balboa 258 Green Meadows 257 East Hollywood 256 90804: Long Beach 251 Valley Village 251 West Whittier/Los Nietos 250 San Fernando 247 90810: Long Beach 245 La Puente 240 90807: Long Beach 236 Glassell Park 236 Chatsworth 235 Vermont Knolls 235 90802: Long Beach 234 La Mirada 234 Little Armenia 230 Monterey Park 230 Palms 227 Hacienda Heights 226 Eagle Rock 225 Silver Lake 220 Baldwin Hills 215 Mission Hills 209 Woodland Hills 208 Downtown 204 Harbor Gateway 204 West Hollywood 203 Hyde Park 202 Rowland Heights 197 Glendora 196 Walnut Park 196 Monrovia 194 Temple City 193 Little Bangladesh 192 Altadena 186 San Gabriel 186 South El Monte 184 Historic Filipinotown 183 Sunland 182 Harvard Heights 180 West Hills 180 Beverly Hills 176 Culver City 176 Valley Glen 175 Rosemead 167 Redondo Beach 165 Valinda 164 Lawndale 162 90815: Long Beach 160 Bassett 160 Cerritos 160 Encino 158 Duarte 154 Lakeview Terrace 153 Lennox 149 Harbor City 145 Tujunga 143 Mid-city 140 South Pasadena 137 San Jose Hills 135 90803: Long Beach 133 Commerce 132 Mt. Washington 132 West Carson 127 Westchester 125 East Rancho Dominguez 121 Hawaiian Gardens 120 Country Club Park 118 Vermont Square 118 Arcadia 110 Porter Ranch 109 Rancho Palos Verdes 109 Santa Fe Springs 103 Unincorporated - Azusa 103 West Los Angeles 103 Calabasas 98 Cloverdale/Cochran 98 Crestview 98 Hancock Park 98 Crenshaw District 96 Del Rey 96 90808: Long Beach 95 Mar Vista 95 Westwood 95 Gramercy Place 93 Brentwood 92 Diamond Bar 92 San Dimas 92 Carthay 90 Hollywood Hills 90 Manhattan Beach 89 Studio City 89 Alsace 87 Elysian Valley 75 Northeast San Gabriel 75 Leimert Park 74 Unincorporated - Covina 74 Venice 74 Adams-Normandie 73 Claremont 71 Victoria Park 70 Jefferson Park 69 Figueroa Park Square 68 Covina (Charter Oak) 65 Los Feliz 65 Artesia 64 Echo Park 64 Walnut 63 West Puente Valley 63 El Camino Village 62 Pacific Palisades 62 South San Gabriel 60 Lomita 58 90814: Long Beach 57 Atwater Village 57 La Canada Flintridge 57 Signal Hill 54 St Elmo Village 54 Athens Village 48 Miracle Mile 48 Quartz Hill 48 La Verne 47 Palos Verdes Estates 46 Avocado Heights 45 Beverlywood 45 Hermosa Beach 45 View Park/Windsor Hills 45 Beverly Crest 43 La Rambla 43 Thai Town 42 Century City 41 Malibu 41 Stevenson Ranch 41 South Carthay 40 Agoura Hills 39 Bel Air 39 Canyon Country 39 El Segundo 39 La Crescenta-Montrose 37 Cadillac-Corning 36 Playa Vista 36 Little Tokyo 35 Wellington Square 35 Lake Los Angeles 33 Manchester Square 33 Reseda Ranch 33 Longwood 32 Rosewood/West Rancho Dominguez 30 Chinatown 28 Santa Monica Mountains 28 Littlerock/Pearblossom 27 Sun Village 26 Ladera Heights 25 Lafayette Square 25 East La Mirada 24 North Whittier 24 Park La Brea 24 San Marino 24 Unincorporated - West LA 24 Cheviot Hills 23 Toluca Lake 23 Unincorporated - Monrovia 23 Val Verde 23 Rancho Dominguez 22 Unincorporated - Duarte 22 Angelino Heights 21 Sierra Madre 21 Wiseburn 21 Rancho Park 20 Del Aire 18 Elysian Park 18 Rolling Hills Estates 18 Acton 17 East Whittier 17 Littlerock 17 Exposition 16 Marina del Rey 16 University Hills 16 White Fence Farms 16 Marina Peninsula 15 Reynier Village 15 Unincorporated - Whittier 15 Shadow Hills 13 Unincorporated - Arcadia 13 Industry 12 La Habra Heights 12 Valencia 12 View Heights 11 Faircrest Heights 10 Regent Square 10 Unincorporated - Hawthorne 10 Unincorporated - South El Monte 10 West Rancho Dominguez 10 Agua Dulce 9 Harbor Pines 9 Kagel/Lopez Canyons 8 Palisades Highlands 8 Rosewood 8 Sunrise Village 8 Toluca Terrace 8 Twin Lakes/Oat Mountain 7 Westlake Village 7 Desert View Highlands 6 North Lancaster 6 Unincorporated - La Verne 6 Unincorporated - Palmdale 6 Irwindale 5 Playa Del Rey 5 Saugus 5 Vernon 5 Bradbury 4 East Pasadena 4 Lake Manor 4 Pearblossom/Llano 4 Unincorporated - Pomona 4 Elizabeth Lake 3 Hidden Hills 3 Leona Valley 3 Mandeville Canyon 3 Santa Catalina Island 3 Toluca Woods 3 Unincorporated - Glendora 3 Anaverde 2 Del Sur 2 Littlerock/Juniper Hills 2 Rolling Hills 2 Rosewood/East Gardena 2 San Pasqual 2 Unincorporated - Angeles National Forest 2 West Antelope Valley 2 Angeles National Forest 1 Bouquet Canyon 1 Brookside 1 Hi Vista 1 Lake Hughes 1 Llano 1 Newhall 1 Pellissier Village 1 Roosevelt 1 South Antelope Valley 1 Sycamore Square 1 Unincorporated - Del Rey 1 Show less Learn more about your county Explore the latest data by visiting our dedicated pages for Los Angeles, Alameda, Contra Costa, Fresno, Imperial, Kern, Kings, Monterey, Orange, Riverside, Sacramento, San Diego, San Bernardino, San Francisco, San Joaquin, San Mateo, Santa Barbara, Santa Clara, Sonoma, Tulare and Ventura counties. Hospitals and patients One goal of the state's stay-at-home mandate is to slow the virus in hope of preventing hospitals from being overrun. To keep tabs on capacity, the state health department tracks hospitalizations of confirmed and suspected COVID-19 patients. All casesConfirmedSuspected Intensive care and other hospitalized patients AprilMayJune01,0002,0003,0004,0005,0006,000 California Department of Public Health In order to reopen, counties must show that hospitalizations have stabilized, meaning that daily increases have averaged less 5% over a seven-day period, or that a county can’t have more than 20 hospitalizations on any single day over a 14-day period. Currently, 52 of the state's 58 counties pass the test. County ICU Other Total Status Los Angeles » 499 1,448 1,947 Declining Orange » 169 260 429 Stable San Diego » 142 201 343 Declining Riverside » 83 258 341 Stable San Bernardino » 90 203 293 Stable Alameda » 48 85 133 Declining Kern » 41 69 110 Stable Fresno » 24 79 103 Stable Ventura » 22 70 92 Stable Imperial » 21 64 85 Stable Stanislaus 19 45 64 Climbing San Joaquin » 20 41 61 Declining Sacramento » 21 36 57 Stable Tulare » 11 42 53 Climbing Santa Barbara » 14 36 50 Climbing Santa Clara » 15 33 48 Declining Kings » 10 37 47 Climbing San Francisco » 8 34 42 Declining Contra Costa » 13 27 40 Declining San Mateo » 7 21 28 Declining Solano 7 18 25 Declining Sonoma » 3 15 18 Climbing Placer 5 13 18 Declining Monterey » 2 15 17 Stable San Luis Obispo 7 6 13 Low Napa 1 9 10 Low Marin 1 8 9 Declining Merced 3 5 8 Low Madera 3 5 8 Declining Humboldt 1 7 8 Low Yuba 2 4 6 Low Santa Cruz 0 4 4 Low Butte 3 0 3 Climbing Lake 1 1 2 Low Yolo 0 1 1 Low El Dorado 1 0 1 Low Glenn 0 1 1 Stable Siskiyou 1 0 1 Low Nevada 0 1 1 Low Amador 0 1 1 Low Colusa 0 1 1 Low San Benito 0 0 0 Low Sutter 0 0 0 Low Tehama 0 0 0 Low Mendocino 0 0 0 Low Calaveras 0 0 0 Low Shasta 0 0 0 Low Tuolumne 0 0 0 Low Inyo 0 0 0 Low Lassen 0 0 0 Low Del Norte 0 0 0 Low Mariposa 0 0 0 Low Plumas 0 0 0 Low Trinity 0 0 0 Low Alpine 0 0 0 Low Modoc 0 0 0 Low Mono 0 0 0 Low Sierra 0 0 0 Low Show less Healthcare workers have been hit hard by the virus. Statewide 12,295 have tested positive as of Tuesday, accounting for 8% of total infections. The number has continued to grow since state officials started releasing tallies. Confirmed cases among healthcare workers MayJune02,0004,0006,0008,00010,00012,000 California Department of Public Health Testing After a fitful start, Gov. Newsom has promised to dramatically increase coronavirus testing in the state. The governor’s goal is to reach at least 60,000 tests per day. Over the last week, an average of 64,501 tests have been conducted each day. New tests by day May 2May 8May 14May 20May 26June 1June 7June 13020,00040,00060,00080,000Governor's daily goal California Department of Public Health As tests have become more widely available, a smaller share are coming back positive. That’s because more people without symptoms have been able to get tested, skewing the trend downward. In the last seven days, about 4.4% of the 451,510 tests conducted have returned a positive result. Positive test rate, seven-day average May 2May 9May 16May 23May 30June 6June 130%2%4%6%8% California Department of Public Health Demographics While Californians of all ages have tested positive for COVID-19, deaths attributed to the virus have tilted heavily toward the elderly. Percentage of cases by age 0-1718-3435-4950-6465-7980+0%10%20%30%40%50% Percentage of deaths by age 0-1718-3435-4950-6465-7980+0%10%20%30%40%50% California Department of Public Health Among most age groups, and especially younger people, Blacks and Latinos are dying more often than other races relative to their share of the population. Percentage of population vs. deaths Age: All 18+ 0-17 18-34 35-49 50-64 65-79 80+ CasesDeaths 0%20%40%60%80%OtherBlackAsianWhiteLatino Race Deaths Deaths Pct. Population Pct. Latino 2,003 40.4% 36.3% White 1,646 33.2% 38.8% Asian 726 14.6% 16.2% Black 474 9.6% 6.1% Other 86 1.7% 2.2% Nursing homes Nursing homes are a tragic focal point of the coronavirus outbreak. At least 2,613 residents have died from COVID-19, 51% of the statewide total. CasesDeaths Deaths at nursing homes vs. elsewhere May 2May 9May 16May 23May 30June 6June 1301,0002,0003,0004,0005,000 California Department of Public Health California's Department of Public Health is currently listing 756 skilled nursing and 108 assisted-living facilities across the state with COVID-19 outbreaks. Filter by countyAlameda Butte Contra Costa El Dorado Fresno Humboldt Imperial Kern Kings Los Angeles Madera Marin Merced Monterey Napa Orange Placer Riverside Sacramento San Bernardino San Diego San Francisco San Joaquin San Luis Obispo San Mateo Santa Barbara Santa Clara Santa Cruz Shasta Solano Sonoma Stanislaus Sutter Tulare Tuolumne Ventura Yolo Residents Staff Facility Cases Deaths Cases Deaths AFFINITY HEALTHCARE CENTER 32 14 33 - ALAMEDA CARE CENTER 50 21 37 10 or fewer ALAMITOS BELMONT REHABILITATION HOSPITAL - - 10 or fewer - ALCOTT REHABILITATION HOSPITAL - - - 10 or fewer ALDEN TERRACE CONVALESCENT HOSPITAL 106 22 36 - ALEXANDRIA CARE CENTER 46 23 27 - ALHAMBRA HEALTHCARE & WELLNESS CENTRE, LP 10 or fewer - 10 or fewer - ALHAMBRA HOSPITAL MEDICAL CENTER D/P SNF - - 10 or fewer - ANGELS NURSING HEALTH CENTER - - 10 or fewer - ANTELOPE VALLEY CARE CENTER 10 or fewer - 10 or fewer - ARARAT CONVALESCENT HOSPITAL 10 or fewer 10 or fewer 16 - ARARAT NURSING FACILITY 10 or fewer - 10 or fewer - ARBOR GLEN CARE CENTER 54 10 or fewer 19 - ASTORIA NURSING AND REHAB CENTER 80 12 61 - ATHERTON BAPTIST HOME-SAM B. WEST 10 or fewer 10 or fewer 10 or fewer - ATLANTIC MEMORIAL HEALTHCARE CENTER 41 10 or fewer 16 - AUTUMN HILLS HEALTH CARE CENTER 56 13 24 - ARARAT HOME OF LOS ANGELES 10 or fewer 10 or fewer 10 or fewer - ARBOR VISTA 10 or fewer 10 or fewer 10 or fewer - ATRIA PARK OF PACIFIC PALISADES 10 or fewer 10 or fewer 10 or fewer - AVANTGARDE SENIOR LIVING OF TARZANA 17 10 or fewer 22 - BALDWIN GARDENS NURSING CENTER 19 10 or fewer 10 or fewer - BAY CREST CARE CENTER - 10 or fewer 10 or fewer - BEACHSIDE POST ACUTE 10 or fewer 10 or fewer 10 or fewer - BEACHWOOD POST-ACUTE & REHAB 46 10 or fewer 19 10 or fewer BEACON HEALTHCARE CENTER 11 - 10 or fewer - BEL TOOREN VILLA CONVALESCENT HOSPITAL 204 10 or fewer 13 10 or fewer BEL VISTA HEALTHCARE CENTER - - - 10 or fewer BELL CONVALESCENT HOSPITAL 51 27 29 - BELLFLOWER POST ACUTE - - 10 or fewer - BERKLEY VALLEY CONVALESCENT HOSPITAL 10 or fewer - 10 or fewer - BEVERLY WEST HEALTHCARE 17 10 or fewer 10 or fewer 10 or fewer BIXBY KNOLLS TOWERS HEALTH CARE & REHAB CENTER 10 or fewer 10 or fewer 10 or fewer - BONNIE BRAE SKILLED NURSING 17 10 or fewer 15 - BRIARCREST NURSING CENTER - - 10 or fewer - BRIER OAK ON SUNSET 12 10 or fewer - - BRIGHTON CARE CENTER 77 16 61 - BROADWAY BY THE SEA 63 16 34 - BROADWAY MANOR CARE CENTER 10 or fewer 10 or fewer 10 or fewer - BROOKDALE NORTHRIDGE - - 10 or fewer - BROOKFIELD HEALTHCARE CENTER 74 10 or fewer 10 or fewer - BUENA VENTURA POST ACUTE CARE CENTER 69 15 22 - BURBANK HEALTHCARE AND REHABILITATION CENTER 65 18 33 - BURLINGTON CONVALESCENT HOSPITAL 34 10 or fewer 17 - BELMONT VILLAGE ENCINO 25 10 or fewer 24 - BELMONT VILLAGE HOLLYWOOD 20 10 or fewer 17 - BELMONT VILLAGE RANCHO PALOS VERDES 10 or fewer 10 or fewer 10 or fewer - BEVERLY HILLS CARMEL RETIREMENT HOTEL 15 10 or fewer 10 or fewer - BEVERLY HILLS GARDENS CARE CENTER 12 10 or fewer 10 or fewer 10 or fewer BROOKDALE CENTRAL WHITTIER 10 or fewer - 10 or fewer - BROOKDALE MONROVIA 10 or fewer 10 or fewer 10 or fewer - BROOKDALE NORTHRIDGE 22 13 10 or fewer - CALIFORNIA HEALTHCARE AND REHABILITATION CENTER 51 11 46 10 or fewer CALIFORNIA POST ACUTE 24 10 or fewer 20 - CALIFORNIA POST-ACUTE CARE 49 10 or fewer 23 - CAMELLIA GARDENS CARE CENTER 10 or fewer 10 or fewer 10 or fewer 10 or fewer CANYON OAKS NURSING AND REHABILITATION CENTER 10 or fewer 10 or fewer 10 or fewer - CASITAS CARE CENTER 56 10 or fewer 38 - CATERED MANOR NURSING CENTER 22 10 or fewer 25 - CENTINELA SKILLED NURSING & WELLNESS CENTRE 23 10 or fewer 17 - CENTURY VILLA, INC. 65 11 38 - CHANDLER CONVALESCENT HOSPITAL 26 10 or fewer 13 10 or fewer CHATSWORTH PARK HEALTH CARE CENTER 34 10 or fewer 28 - CHINO VALLEY HEALTH CARE CENTER - - 10 or fewer - CLARA BALDWIN STOCKER HOME 10 or fewer 10 or fewer 11 - CLAREMONT CARE CENTER - - 10 or fewer - CLAREMONT MANOR CARE CENTER - - 10 or fewer - CLEAR VIEW SANITARIUM - - 10 or fewer - COLLEGE VISTA POST-ACUTE - 10 or fewer 10 or fewer - COLONIAL CARE CENTER 66 10 or fewer 35 - COLONIAL GARDENS NURSING HOME 75 10 or fewer 27 10 or fewer COMMUNITY CARE CENTER 10 or fewer - 10 or fewer - COUNTRY MANOR HEALTHCARE 10 or fewer 10 or fewer 10 or fewer - COUNTRY VILLA BAY VISTA HEALTHCARE CENTER 10 or fewer - 10 or fewer - COUNTRY VILLA BELMONT HEIGHTS HEALTHCARE CENTER 10 or fewer - 10 or fewer - COUNTRY VILLA CLAREMONT HEALTHCARE CENTER 10 or fewer - 10 or fewer - COUNTRY VILLA EAST NURSING CENTER 92 13 19 - COUNTRY VILLA LOS FELIZ NURSING CENTER 10 or fewer - 10 or fewer - COUNTRY VILLA MAR VISTA NURSING CENTER 10 or fewer 10 or fewer 11 - COUNTRY VILLA NORTH CONVALESCENT CENTER 10 or fewer 10 or fewer 10 or fewer - COUNTRY VILLA PAVILION NURSING CENTER 77 10 or fewer 22 10 or fewer COUNTRY VILLA REHABILITATION CENTER 24 10 or fewer 16 - COUNTRY VILLA SHERATON NURSING AND REHAB. CENTER 59 18 25 10 or fewer COUNTRY VILLA SOUTH CONVALESCENT CENTER 171 19 16 - COUNTRY VILLA TERRACE NURSING CENTER 10 or fewer - 13 - COUNTRY VILLA WESTWOOD CONVALESCENT CENTER 10 or fewer - 17 - COUNTRY VILLA WILSHIRE CONVALESCENT CENTER 10 or fewer - 10 or fewer - COURTYARD CARE CENTER 10 or fewer - 10 or fewer - COVINA REHABILITATION CENTER 17 10 or fewer 22 - CRENSHAW NURSING HOME 10 or fewer - - - CULVER WEST HEALTH CENTER 39 10 or fewer 20 - CANYON TRAILS AT TOPANGA SENIOR LIVING 18 10 or fewer 10 or fewer - CARSON SENIOR ASSISTED LIVING 10 or fewer 10 or fewer 10 or fewer - CEDARS ASSISTED LIVING, THE 38 10 or fewer 12 - CITY VIEW LA, LLC 10 or fewer - 10 or fewer - CROFTON MANOR INN 28 10 or fewer 16 - DEL AMO GARDENS CARE CENTER - - 10 or fewer - DEL RIO CONVALESCENT CENTER 30 10 or fewer 10 or fewer - DEL RIO GARDENS CARE CENTER 21 10 or fewer 10 or fewer - DESERT CANYON POST ACUTE, LLC - 10 or fewer 10 or fewer - DOWNEY COMMUNITY HEALTH CENTER 93 10 or fewer 42 - DOWNEY POST ACUTE 39 10 or fewer 20 - EAST LOS ANGELES DOCTORS HOSPITAL D/P SNF 10 or fewer - 10 or fewer - EASTLAND SUBACUTE AND REHABILITATION CENTER 10 or fewer - 10 or fewer - EDGEWATER SKILLED NURSING CENTER - - 10 or fewer - EISENBERG VILLAGE 16 10 or fewer 24 - EL ENCANTO HEALTHCARE AND HABILITATION CENTER - 10 or fewer 10 or fewer - EL MONTE CONVALESCENT HOSPITAL - - 10 or fewer - EL RANCHO VISTA HEALTH CARE CENTER 46 20 19 10 or fewer ELMCREST CARE CENTER 42 11 15 10 or fewer EMERALD TERRACE CONVALESCENT HOSPITAL 20 10 or fewer 10 or fewer 10 or fewer ENCINO HOSPITAL MEDICAL CENTER - - 10 or fewer - FIRESIDE HEALTH CARE CENTER - - 10 or fewer - FLOWER VILLA, INC. 18 10 or fewer 14 10 or fewer FOOTHILL HEIGHTS CARE CENTER 19 10 or fewer 10 or fewer - FOUNTAIN VIEW SUBACUTE AND NURSING CENTER 10 or fewer - 10 or fewer - FOUR SEASONS HEALTHCARE & WELLNESS CENTER, LP 96 14 54 - FALLBROOK GLEN OF WEST HILLS 19 10 or fewer 10 or fewer - GARDEN VIEW POST-ACUTE REHABILITATION - - 10 or fewer - GARDENA CONVALESCENT CENTER 36 10 or fewer 22 - GEM TRANSITIONAL CARE CENTER 47 15 22 - GLENDALE ADVENTIST MEDICAL CENTER D/P SNF 24 10 or fewer 10 or fewer - GLENDALE HEALTHCARE CENTER 11 10 or fewer 11 - GLENDALE POST ACUTE CENTER 10 or fewer 10 or fewer 10 or fewer - GLENDORA CANYON TRANSITIONAL CARE UNIT - - 10 or fewer - GLENDORA GRAND, INC. 10 or fewer - 10 or fewer - GLENHAVEN HEALTHCARE 19 10 or fewer 16 - GLENOAKS CONV. HOSPITAL 27 10 or fewer 10 or fewer - GOLDEN LEGACY CARE CENTER 10 or fewer - 10 or fewer - GOLDEN STATE COLONIAL HEALTHCARE CENTER - - 10 or fewer - GOOD SHEPHERD HEALTH CARE CENTER OF SANTA MONICA 10 or fewer - 10 or fewer - GRANADA HILLS CONVALESCENT HOSPITAL 10 or fewer 10 or fewer 10 or fewer 10 or fewer GRANADA POST ACUTE 47 12 20 - GRANCELL VILLAGE OF THE JEWISH HOMES FOR THE AGING 10 or fewer - 10 or fewer - GRAND PARK CONVALESCENT HOSPITAL 62 27 33 - GRAND VALLEY HEALTH CARE CENTER - - 10 or fewer - GREATER EL MONTE COMMUNITY HOSPITAL D/P SNF - - 10 or fewer - GREEN ACRES HEALTHCARE CENTER 10 or fewer 10 or fewer 15 - GREENFIELD CARE CENTER OF SOUTH GATE 37 10 or fewer 14 - GRIFFITH PARK HEALTHCARE CENTER 10 or fewer - 10 or fewer - GUARDIAN REHABILITATION HOSPITAL 10 or fewer - 10 or fewer - GOLDEN ASSISTED LIVING 12 - 10 or fewer - GRANDVIEW, THE 18 10 or fewer 10 or fewer - HARBOR POST ACUTE CARE CENTER 37 10 or fewer 18 - HAWTHORNE HEALTHCARE & WELLNESS CENTRE, LP 32 10 or fewer 11 - HERITAGE REHABILITATION CENTER 10 or fewer - 10 or fewer - HIGH VALLEY LODGE 17 10 or fewer 10 or fewer - HIGHLAND PARK SKILLED NURSING & WELLNESS CENTRE 10 or fewer - 10 or fewer - HOLIDAY MANOR CARE CENTER - - 10 or fewer - HOLLENBECK PALMS 10 or fewer - 10 or fewer - HOLLYWOOD PREMIER HEALTHCARE CENTER 29 10 or fewer 10 or fewer - HUNTINGTON DRIVE HEALTH AND REHABILITATION CENTER 10 or fewer - 10 or fewer - HUNTINGTON HEALTHCARE CENTER 22 14 18 - HUNTINGTON POST ACUTE 62 10 or fewer 23 - HYDE PARK HEALTHCARE CENTER 12 10 or fewer 10 or fewer - HEIGHTS AT BURBANK, THE 10 or fewer 10 or fewer 10 or fewer - IMPERIAL CREST HEALTH CARE CENTER 10 or fewer 10 or fewer 10 or fewer - IMPERIAL HEALTHCARE CENTER 51 10 or fewer 19 - INFINITY CARE OF EAST LOS ANGELES 10 or fewer 10 or fewer 15 - INGLEWOOD HEALTH CARE CENTER 42 10 or fewer 16 - INLAND VALLEY CARE AND REHABILITATION CENTER 10 or fewer - 10 or fewer - INTERCOMMUNITY CARE CENTER 10 or fewer - 10 or fewer - INTERCOMMUNITY HEALTHCARE & REHABILITATION CENTER - - 10 or fewer - IVY CREEK HEALTHCARE & WELLNESS CENTRE - - 10 or fewer - JOYCE EISENBERG KEEFER MEDICAL CENTER 10 or fewer - 10 or fewer - JASMIN TERRACE AT EL MOLINO 65 16 25 - KEI-AI LOS ANGELES HEALTHCARE CENTER 80 22 46 - KEI-AI SOUTH BAY HEALTHCARE CENTER 44 13 40 - KENNEDY POST ACUTE CARE CENTER 10 or fewer - 10 or fewer - KINGSLEY MANOR CARE CENTER 19 10 or fewer 20 - KENSINGTON REDONDO BEACH, THE 23 10 or fewer 22 - KENSINGTON SIERRA MADRE, THE 10 or fewer - 10 or fewer - KINGSLEY MANOR - - 10 or fewer 10 or fewer LA BREA REHABILITATION CENTER 11 10 or fewer 17 - LA CRESCENTA HEALTHCARE CENTER 19 10 or fewer 15 - LA HABRA CONVALESCENT HOSPITAL - - 10 or fewer - LA PAZ GEROPSYCHIATRIC CENTER 10 or fewer - 10 or fewer - LAKE BALBOA CARE CENTER 10 or fewer - 10 or fewer - LAKEVIEW TERRACE 34 10 or fewer 10 or fewer - LAKEWOOD HEALTHCARE CENTER 58 10 or fewer 49 - LANDMARK MEDICAL CENTER 33 - 23 - LAS FLORES CONVALESCENT HOSPITAL 10 or fewer 10 or fewer 10 or fewer - LAUREL PARK BEHAVIORAL HEALTH CENTER 10 or fewer - - - LAWNDALE HEALTHCARE & WELLNESS CENTRE, LLC 10 or fewer - 10 or fewer - LEGACY HEALTHCARE CENTER 22 - 10 or fewer - LEISURE GLEN POST ACUTE CARE CENTER 23 10 or fewer 22 - LIGHTHOUSE HEALTHCARE CENTER 10 or fewer - 10 or fewer - LIVE OAK REHABILITATION CENTER - - 10 or fewer - LOMITA POST-ACUTE CARE CENTER - - 10 or fewer - LONG BEACH CARE CENTER 56 10 or fewer 29 10 or fewer LONG BEACH HEALTHCARE CENTER 29 10 or fewer 10 or fewer 10 or fewer LONGWOOD MANOR CONVALESCENT HOSPITAL 11 10 or fewer - - LOS ANGELES COMMUNITY HOSPITAL D/P SNF - - 10 or fewer - LOS PALOS POST-ACUTE CARE CENTER 33 - 10 or fewer - LYNWOOD HEALTHCARE CENTER 41 13 29 - LAKEWOOD PARK MANOR 10 or fewer 10 or fewer 10 or fewer - MACLAY HEALTHCARE CENTER 55 12 60 - MAGNOLIA GARDENS CONVALESCENT HOSPITAL 31 10 or fewer 15 10 or fewer MANCHESTER MANOR CVLT HOSPITAL 10 or fewer - - - MARINA POINTE HEALTHCARE & SUBACUTE 10 or fewer 10 or fewer 10 or fewer - MARLORA POST ACUTE REHABILITATION HOSPITAL - - 10 or fewer - MAYFLOWER CARE CENTER 10 or fewer - 10 or fewer - MAYFLOWER GARDENS CONVALESCENT HOSPITAL 21 10 or fewer 12 - MAYWOOD SKILLED NURSING & WELLNESS CENTRE 37 11 32 - MEADOWBROOK BEHAVIORAL HEALTH CENTER 10 or fewer - 10 or fewer - MEMORIAL HOSPITAL OF GARDENA D/P SNF - - 10 or fewer - MESA GLEN CARE CENTER - - 10 or fewer - MID-WILSHIRE HEALTH CARE CENTER 39 12 13 - MISSION CARE CENTER - - 10 or fewer - MONROVIA GARDENS HEALTHCARE CENTER 57 21 23 - MONTE VISTA HEALTHCARE CENTER 46 12 24 - MONTEBELLO CARE CENTER 10 or fewer 10 or fewer - - MONTECITO HEIGHTS HEALTHCARE & WELLNESS CENTRE, LP. 10 or fewer - 10 or fewer - MONTEREY HEALTHCARE & WELLNESS CENTRE, LP 10 or fewer - 10 or fewer - MONTEREY PARK CONVALESCENT HOSPITAL 10 or fewer - - - MONTROSE HEALTHCARE CENTER 36 12 22 - MONTROSE SPRINGS SKILLED NURSING & WELLNESS CENTER 15 10 or fewer 13 - MOTION PICTURE & TELEVISION HOSPITAL D/P SNF 17 10 or fewer 17 - MOUNTAIN VIEW CONVALESCENT HOSPITAL 37 16 26 - MONTECEDRO 10 or fewer 10 or fewer 10 or fewer - MUGUNGWHA SILVER TOWN 10 or fewer 10 or fewer 10 or fewer - NEW VISTA NURSING AND REHABILITATION CENTER 45 - 37 10 or fewer NEW VISTA POST-ACUTE CARE CENTER 10 or fewer - 10 or fewer - NORTH VALLEY NURSING CENTER 10 or fewer - - - NORTHRIDGE CARE CENTER 20 10 or fewer 11 10 or fewer NORWALK MEADOWS NURSING CENTER 10 or fewer 10 or fewer 10 or fewer - NORWALK SKILLED NURSING & WELLNESS CENTRE 59 10 or fewer 34 10 or fewer OAKPARK HEALTHCARE CENTER 10 or fewer 10 or fewer - - OCEAN POINTE HEALTHCARE CENTER 33 10 or fewer 11 - OLIVE VISTA BEHAVIORAL HEALTH CENTER - - 10 or fewer - OLYMPIA CONVALESCENT HOSPITAL 62 21 30 - OSAGE HEALTHCARE & WELLNESS CENTRE 25 11 10 or fewer - OAKMONT OF SANTA CLARITA 14 10 or fewer 10 or fewer - OAKMONT OF VALENCIA 15 10 or fewer 10 or fewer - PACIFIC CARE NURSING CENTER 10 or fewer - 10 or fewer - PACIFIC PALMS HEALTHCARE 17 10 or fewer 15 - PACIFIC POST - ACUTE 10 or fewer 10 or fewer 10 or fewer - PACIFIC VILLA, INC. 50 10 or fewer 14 10 or fewer PACIFICA HOSPITAL OF THE VALLEY D/P SNF 10 or fewer 10 or fewer 10 or fewer - PALAZZO POST ACUTE 22 - 12 10 or fewer PALOS VERDES HEALTH CARE CENTER 10 or fewer - 10 or fewer - PANORAMA GARDENS NURSING AND REHABILITATION CENTER 67 15 42 - PARAMOUNT CONVALESCENT HOSPITAL 10 or fewer - 10 or fewer - PARK AVENUE HEALTHCARE & WELLNESS CENTER - - 10 or fewer - PARK REGENCY CARE CENTER - - 10 or fewer - PARKWEST HEALTHCARE CENTER 30 10 or fewer 21 - PASADENA CARE CENTER, LLC 10 or fewer - 10 or fewer - PASADENA GROVE HEALTH CENTER 41 10 or fewer 24 - PASADENA PARK HEALTHCARE AND WELLNESS CENTER 10 or fewer 10 or fewer 12 - PENN MAR THERAPEUTIC CENTER 18 - 10 or fewer - PICO RIVERA HEALTHCARE CENTER 10 or fewer 10 or fewer 10 or fewer - PILGRIM PLACE HEALTH SERVICES CENTER - - 10 or fewer - PLAYA DEL REY CENTER 10 or fewer 10 or fewer 18 - PRESBYTERIAN INTERCOMMUNITY HOSPITAL D/P SNF - - 10 or fewer - PRIMROSE POST-ACUTE 24 10 or fewer 19 - PROVIDENCE HOLY CROSS MEDICAL CENTER D/P SNF 10 or fewer - 10 or fewer - PROVIDENCE LITTLE COMPANY OF MARY SUBACUTE CARE CENTER 10 or fewer 10 or fewer 15 - PROVIDENCE LITTLE COMPANY OF MARY TRANSITIONAL CARE CENTER 10 or fewer 10 or fewer 10 or fewer - PROVIDENCE ST. ELIZABETH CARE CENTER - - 10 or fewer - PACIFICA SENIOR LIVING NORTHRIDGE 41 15 26 - PALMCREST GRAND RESIDENCE 15 10 or fewer 10 or fewer - RAMONA NURSING & REHABILITATION CENTER 46 10 or fewer 29 - REGENCY OAKS POST ACUTE CARE CENTER - - 10 or fewer - RINALDI CONVALESCENT HOSPITAL 25 10 or fewer 16 - RIO HONDO SUBACUTE & NURSING CENTER 27 - 10 or fewer 10 or fewer RIVIERA HEALTHCARE CENTER 87 11 24 - ROSE GARDEN HEALTHCARE CENTER 33 10 or fewer 34 - ROSE VILLA HEALTHCARE CENTER 10 or fewer - 10 or fewer - ROSECRANS CARE CENTER 10 or fewer - 10 or fewer - ROYAL CARE SKILLED NURSING CENTER 11 10 or fewer 12 - ROYAL CREST HEALTH CARE - - 10 or fewer - ROYAL GARDENS HEALTHCARE 10 or fewer - 10 or fewer - ROYAL OAKS MANOR - BRADBURY OAKS - - 10 or fewer - ROYAL PALMS POST ACUTE 10 or fewer - 15 - ROYAL TERRACE HEALTH CARE - - 10 or fewer - ROYAL VISTA CARE CENTER 31 10 or fewer 17 10 or fewer ROYALWOOD CARE CENTER 65 15 43 - ROSECRANS VILLA RESIDENTIAL CARE 14 - 10 or fewer - SAINT VINCENT HEALTHCARE 13 - 14 10 or fewer SAN GABRIEL CONVALESCENT CENTER - - 10 or fewer - SANTA ANITA CONVALESCENT HOSPITAL 58 23 23 - SANTA CLARITA POST-ACUTE CARE CENTER 10 or fewer 10 or fewer 10 or fewer - SANTA FE HEIGHTS HEALTHCARE CENTER LLC 10 or fewer - 10 or fewer - SANTA FE LODGE 17 10 or fewer 10 or fewer - SANTA MONICA CONVALESCENT CENTER II 21 - 25 - SANTA MONICA HEALTH CARE CENTER 15 10 or fewer 15 - SANTA TERESITA MANOR 16 10 or fewer 13 - SEACREST POST-ACUTE CARE CENTER 25 - - - SHADOW HILLS CONVALESCENT HOSPITAL 39 10 or fewer 27 10 or fewer SHARON CARE CENTER 10 or fewer - 10 or fewer - SHERMAN OAKS HEALTH & REHABILITATION CENTER 14 10 or fewer 10 or fewer - SHERMAN VILLAGE HEALTHCARE CENTER - - 12 - SHORELINE HEALTHCARE CENTER 10 or fewer - 10 or fewer - SIERRA VIEW CARE CENTER 10 or fewer - 10 or fewer - SKYLINE HEALTHCARE CENTER-LOS ANGELES 10 or fewer - 12 - SOCAL POST-ACUTE CARE - - 10 or fewer - SOLHEIM SENIOR COMMUNITY 10 or fewer 10 or fewer 12 - SOUTH PASADENA CARE CENTER 82 15 29 - SOUTHLAND 10 or fewer - 10 or fewer - ST. JOHN OF GOD RETIREMENT AND CARE CENTER 10 or fewer - 22 - STONEY POINT HEALTHCARE CENTER 33 12 32 - STUDIO CITY REHABILITATION CENTER - 10 or fewer 10 or fewer - SUNNY VILLAGE CARE CENTER - - 10 or fewer - SUNNYSIDE NURSING CENTER 10 or fewer - 10 or fewer - SUNNYVIEW CARE CENTER 26 12 12 10 or fewer SUNRAY HEALTHCARE CENTER 40 15 14 10 or fewer SUNSET MANOR CONVALESCENT HOSPITAL 10 or fewer - 10 or fewer - SYLMAR HEALTH AND REHABILITATION CENTER 41 - 33 - SILVERADO SENIOR LIVING - CALABASAS 49 10 or fewer 17 - SILVERADO SENIOR LIVING – BEVERLY PLACE 57 13 33 10 or fewer SOLHEIM SENIOR COMMUNITY 10 or fewer 10 or fewer 10 or fewer - SUNRISE ASSISTED LIVING OF STUDIO CITY 10 or fewer 10 or fewer 10 or fewer - SUNRISE VILLA CULVER CITY 10 or fewer 10 or fewer 10 or fewer - TARZANA HEALTH AND REHABILITATION CENTER 77 10 or fewer 36 - TEMPLE CITY HEALTHCARE 10 or fewer - 10 or fewer - TEMPLE PARK CONVALESCENT HOSPITAL - - 10 or fewer - THE CALIFORNIAN - PASADENA CONVALESCENT HOSPITAL - - 10 or fewer - THE CARE CENTER ON HAZELTINE, LLC 10 or fewer - - - THE EARLWOOD 33 10 or fewer 24 - THE ELLISON JOHN TRANSITIONAL CARE CENTER 10 or fewer 10 or fewer 10 or fewer - THE GARDENS OF EL MONTE 27 10 or fewer 12 - THE GROVE POST-ACUTE CARE CENTER 43 12 17 - THE MEADOWS POST ACUTE 49 15 25 10 or fewer THE ORCHARD - POST ACUTE CARE 33 10 or fewer 10 or fewer - THE REHABILITATION CENTER OF SANTA MONICA 37 12 24 - THE REHABILITATION CENTRE OF BEVERLY HILLS 29 10 or fewer 36 - THE ROWLAND 10 or fewer - 10 or fewer - TOPANGA TERRACE - - 17 - TORRANCE CARE CENTER WEST, INC. 55 12 17 10 or fewer TORRANCE MEMORIAL MEDICAL CENTER D/P SNF 10 or fewer - 10 or fewer - UNIVERSITY PARK HEALTHCARE CENTER 23 10 or fewer 14 - VALLEY PALMS CARE CENTER 28 10 or fewer 10 or fewer - VALLEY VISTA NURSING AND TRANSITIONAL CARE LLC 10 or fewer - 10 or fewer - VERMONT HEALTHCARE CENTER 14 12 24 - VERNON HEALTHCARE CENTER 10 or fewer - 10 or fewer - VETERANS HOME OF CALIFORNIA - WEST LOS ANGELES 10 or fewer 10 or fewer 10 or fewer - VICTORIA CARE CENTER - - 11 - VIEW HEIGHTS CONVALESCENT HOSPITAL - - 10 or fewer - VIEW PARK CONVALESCENT CENTER 45 18 41 - VILLA ELENA HEALTHCARE CENTER - 10 or fewer 10 or fewer - VILLA GARDENS HEALTH CARE UNIT 10 or fewer - 10 or fewer - VILLA SCALABRINI SPECIAL CARE UNIT 10 or fewer - 12 - VIRGIL REHABILITATION AND SKILLED NURSING CENTER 21 13 20 - VISTA DEL SOL CARE CENTER 10 or fewer 10 or fewer 14 - VETERANS HOME OF CALIFORNIA – WEST LOS ANGELES 10 or fewer 10 or fewer 10 or fewer - VILLA GARDENS 16 10 or fewer 26 - VISTA DEL MAR SENIOR LIVING 12 - 10 or fewer - WELLSPRINGS POST-ACUTE CENTER - - 10 or fewer - WEST COVINA HEALTHCARE CENTER - - 10 or fewer - WEST COVINA MEDICAL CENTER D/P SNF - - 10 or fewer - WEST HAVEN HEALTHCARE 41 10 or fewer 15 - WEST HILLS HEALTH AND REHABILITATION CENTER 77 28 39 - WEST VALLEY POST ACUTE 65 21 22 10 or fewer WESTERN CONVALESCENT HOSPITAL 10 or fewer - 10 or fewer 10 or fewer WESTLAKE CONVALESCENT HOSPITAL 10 or fewer - 10 or fewer - WHITTIER HILLS HEALTH CARE CENTER 10 or fewer 10 or fewer 12 - WHITTIER PACIFIC CARE CENTER 18 10 or fewer 10 or fewer - WINDSOR CARE CENTER OF CHEVIOT HILLS 53 10 or fewer 31 - WINDSOR CONVALESCENT CENTER OF NORTH LONG BEACH 10 or fewer 10 or fewer 10 or fewer - WINDSOR GARDENS CONVALESCENT CENTER OF HAWTHORNE 14 10 or fewer 10 or fewer - WINDSOR GARDENS CONVALESCENT CENTER OF LONG BEACH 10 or fewer - 10 or fewer - WINDSOR GARDENS CONVALESCENT HOSPITAL 10 or fewer - 10 or fewer - WINDSOR GARDENS HEALTHCARE CENTER OF THE VALLEY 10 or fewer - 10 or fewer - WINDSOR MANOR - - 10 or fewer - WINDSOR PALMS CARE CENTER OF ARTESIA - - 10 or fewer - WINDSOR TERRACE HEALTHCARE CENTER 39 10 or fewer 35 - WOODLAND CARE CENTER 25 10 or fewer 10 or fewer 10 or fewer WOODRUFF CONVALESCENT CENTER 10 or fewer - 10 or fewer - WEST VALLEY ASSISTED LIVING 10 or fewer 10 or fewer - - WINDSOR HALL CARE HOME INC. 10 or fewer 10 or fewer 10 or fewer - YORK HEALTHCARE & WELLNESS CENTRE 34 10 or fewer 22 - Show less The state last updated the list on June 16. Officials have withheld the precise number where there are 10 or fewer cases. The totals are cumulative counts. Lives lost to COVID-19 Learn more about those we've lost by reading Times obituaries of Californians who have died from coronavirus. Reopening the state California is moving into the third stage of Gov. Gavin Newsom's four-phase plan to gradually reopen the state amid the COVID-19 pandemic. Newsom's roadmap for reopening Stage 1 March 19 - May 7 Strictest restrictions in place. Stage 2 Began May 8 Lower-risk businesses can reopen with social distancing guidelines. Advanced Stage 2: Retail and dine-in restaurants are reopened with social distancing guidelines Stage 3 Began June 12 Higher-risk businesses and venues (such as movie theaters and gyms) can reopen with social distancing guidelines. Stage 4 No date set Concerts, conventions and sports with a live crowd can reopen. All of California's 58 counties have moved into Stage 2, including Los Angeles County. No restrictions lifted All restrictions lifted Hover for more information. Los AngelesLos AngelesSan DiegoSan DiegoSacramentoSacramentoSan FranciscoSan FranciscoReddingRedding What's open in every county We're tracking what's open, closed and restricted throughout the state in ten different categories, including parks, retail, restaurants and more. Find out what's open where you live. Our state in context The coronavirus has hit most of the U.S., with the largest concentrations in and around New York City. California, America's most populous state, has one of the highest totals. It ranks much lower after adjusting for population. State Cases Per 100k New cases FewerMore New York 384,575 1,960.3 Mar 1Jun 16 New Jersey 167,426 1,885 California 159,183 406.6 Illinois 133,639 1,042.3 Massachusetts 105,885 1,550.2 Texas 93,569 335.6 Pennsylvania 83,978 656.5 Florida 80,109 388.9 Michigan 66,269 665.5 Maryland 62,409 1,039.6 Georgia 59,078 573.7 Virginia 55,331 657.6 Louisiana 47,706 1,022.9 North Carolina 45,907 452 Connecticut 45,349 1,266.2 Ohio 42,010 360.9 Indiana 40,786 614.5 Arizona 39,185 564.1 Tennessee 31,751 477.4 Minnesota 30,882 558.7 Colorado 29,427 532 Alabama 26,912 553.2 Washington 26,531 363.7 Iowa 24,179 771.9 Wisconsin 23,198 401.5 Mississippi 20,152 674.3 South Carolina 19,990 403.4 Nebraska 17,038 894.5 Missouri 16,857 276.8 Rhode Island 16,164 1,529.8 Utah 14,937 490.5 Arkansas 13,191 441.1 Kentucky 12,647 284.8 Nevada 11,683 399.7 Kansas 11,411 392.3 Delaware 10,403 1,095.6 New Mexico 9,933 474.7 District of Columbia 9,818 1,434.3 Oklahoma 8,645 220.6 Oregon 6,098 149.4 South Dakota 5,966 690.3 Puerto Rico 5,951 175.7 New Hampshire 5,364 399.2 Idaho 3,462 205.1 North Dakota 3,124 415.3 Maine 2,819 211.5 West Virginia 2,341 128 Vermont 1,131 181 Wyoming 1,089 187.2 Hawaii 736 51.8 Alaska 675 91.4 Montana 614 58.9 Show less No state has had more deaths than New York, though its pace has declined in recent weeks. State Deaths Per 100k New deaths FewerMore New York 30,909 157.6 Mar 1Jun 16 New Jersey 12,792 144.0 Massachusetts 7,664 112.2 Illinois 6,398 49.9 Pennsylvania 6,276 49.1 Michigan 6,034 60.6 California 5,202 13.3 Connecticut 4,210 117.5 Louisiana 3,042 65.2 Florida 2,993 14.5 Maryland 2,982 49.7 Ohio 2,599 22.3 Georgia 2,529 24.6 Indiana 2,447 36.9 Texas 2,049 7.3 Colorado 1,617 29.2 Virginia 1,570 18.7 Minnesota 1,344 24.3 Washington 1,231 16.9 Arizona 1,228 17.7 North Carolina 1,169 11.5 Mississippi 915 30.6 Missouri 891 14.6 Rhode Island 865 81.9 Alabama 785 16.1 Wisconsin 703 12.2 Iowa 665 21.2 South Carolina 607 12.2 District of Columbia 520 76.0 Kentucky 505 11.4 Tennessee 493 7.4 Nevada 467 16.0 New Mexico 447 21.4 Delaware 424 44.7 Oklahoma 363 9.3 New Hampshire 326 24.3 Kansas 246 8.5 Nebraska 231 12.1 Arkansas 188 6.3 Oregon 182 4.5 Puerto Rico 147 4.3 Utah 145 4.8 Maine 101 7.6 Idaho 88 5.2 West Virginia 88 4.8 South Dakota 77 8.9 North Dakota 74 9.8 Vermont 55 8.8 Montana 19 1.8 Wyoming 18 3.1 Hawaii 17 1.2 Alaska 12 1.6 Show less Tracking the coronavirus California counties Alameda Contra Costa Fresno Imperial Kern Kings Los Angeles Monterey Orange Riverside Sacramento San Bernardino San Diego San Francisco San Joaquin San Mateo Santa Barbara Santa Clara Sonoma Tulare Ventura Other trackers Beach closures Housing homeless people Reopenings by county The lives lost More coverage Symptoms How it spreads Get our newsletter About the numbers This page was created by Swetha Kannan, Casey Miller, Sean Greene, Lorena Iñiguez Elebee, Rong-Gong Lin II, Ryan Murphy, Melody Gutierrez, Priya Krishnakumar, Sandhya Kambhampati, Maloy Moore, Jennifer Lu, Aida Ylanan, Vanessa Martínez, Ryan Menezes, Thomas Suh Lauder, Andrea Roberson, Ben Poston, Nicole Santa Cruz, Iris Lee, Rahul Mukherjee, Jaclyn Cosgrove, Anthony Pesce, Paul Duginski and Phi Do. State and county totals come from a continual Times survey of California's 58 county health agencies as well as the three run by cities. Those figures are ahead of the totals periodically released by the state's Department of Public Health. State officials acknowledge that their tallies lag behind the updates posted by local agencies throughout the day and do not dispute The Times' method. The Times switched to using this method on March 18, leading to increases over what it had published previously using state data. The tallies here are mostly limited to residents of California, which is the standard method used to count patients by the state’s health authorities. Those totals do not include people from other states who are quarantined here, such as the passengers and crew of the Grand Princess cruise ship that docked in Oakland. In an effort to aid scientists and researchers in the fight against COVID-19, The Times has released its database of California coronavirus cases to the public. The database is available on Github, a popular website for hosting data and computer code. The files will be updated daily at github.com/datadesk/california-coronavirus-data. Closures and restrictions are drawn from an ongoing Times survey of county governments. National and global case data are collected by the the Centers for Disease Control and Prevention and researchers at the Johns Hopkins University Center for Systems Science and Engineering. If you see information here that you believe is incorrect or out of date, please contact Data and Graphics Editor Ben Welsh at [email protected]. https://www.latimes.com/projects/california-coronavirus-cases-tracking-outbreak/

June 16 http://mediaarchives.gsradio.net/rense/special/rense_061620_hr3.mp3

SEARCHGIVE NOWSUBSCRIBE NEWS EVENTS CALENDAR BLOG CONNECT WITH US PRESS ROOM HOST AN EVENT HOMENEWS & EVENTSPRESS ROOM2020THE D614G MUTATION IN THE SARS-COV-2 SPIKE PROTEIN REDUCES S1 SHEDDING AND INCREASES INFECTIVITY The D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases infectivity Lizhou Zhang1#, Cody B Jackson1#, Huihui Mou1#, Amrita Ojha1, Erumbi S Rangarajan2, Tina Izard2, Michael Farzan1*, Hyeryun Choe1* 1Department of Immunology and Microbial Science, The Scripps Research Institute, Jupiter, FL 33458, USA 2Department of Integrative Structural and Computational Biology, The Scripps Research Institute, Jupiter, Florida 33458 #contributed equally *Correspondence: [email protected]; [email protected] ABSTRACT SARS coronavirus 2 (SARS-CoV-2) isolates encoding a D614G mutation in the viral spike (S) protein predominate over time in locales where it is found, implying that this change enhances viral transmission. We therefore compared the functional properties of the S proteins with aspartic acid (SD614) and glycine (SG614) at residue 614. We observed that retroviruses pseudotyped with SG614 infected ACE2- expressing cells markedly more efficiently than those with SD614. This greater infectivity was correlated with less S1 shedding and greater incorporation of the S protein into the pseudovirion. Similar results were obtained using the virus-like particles produced with SARS-CoV-2 M, N, E, and S proteins. However, SG614 did not bind ACE2 more efficiently than SD614, and the pseudoviruses containing these S proteins were neutralized with comparable efficiencies by convalescent plasma. These results show SG614 is more stable than SD614, consistent with epidemiological data suggesting that viruses with SG614 transmit more efficiently. Until late 2019, only six coronaviruses were known to infect humans: HCoV-229E, HCoV-OC43, SARS-CoV (SARS-CoV-1), HCoV-NL63, CoV-HKU1, and MERS-CoV. A seventh, SARS-CoV-2, emerged in the winter of 2019 from Wuhan, China. SARS- CoV-2 is closely related to SARS-CoV-1, a virus that appeared from Guangdong province, China in late 2002. The coronavirus spike (S) protein mediates receptor binding and fusion of the viral and cellular membrane. The S protein extends from the viral membrane and is uniformly arranged as trimers on the virion surface to give the appearance of a crown (corona in Latin). The coronavirus S protein is divided into two domains: S1 and S2. The S1 domain mediates receptor binding, and the S2 mediates downstream membrane fusion1,2. The receptor for SARS-CoV-2 is angiotensin-converting enzyme 2 (ACE2)3-7, a metalloprotease that also serves as the receptor for SARS-CoV-18. A small, independently folded subdomain of S1, described as the receptor-binding domain (RBD), directly binds ACE2 when the virus engages a target cell9-12. The S1/S2 junction of SARS-CoV-2 is processed by a furin-like proprotein convertase in the virus producer cell. In contrast, the S1/S2 junction of SARS-CoV-1 is processed by TMPRSS2 at the cell surface or by lysosomal cathepsins in the target cells13-18. Both S proteins are further processed in the target cell within the S2 domain at the S2’ site, an event that is also required for productive infection19,20. Recent analyses of the fine-scale sequence variation of SARS-CoV-2 isolates identified several genomic regions of increased genetic variation21-30. One of these variations encodes a S-protein mutation, D614G, in the carboxy(C)-terminal region of the S1 domain21-23,26,30. This region of the S1 domain directly associates with S2 (Fig. 1a). This mutation with glycine at the residue 614 (G614) was previously detected to increase with an alarming speed21,22. Our own analysis of the S-protein sequences available from the GenBank showed a similar result: The G614 genotype was not detected in February (among 33 sequences) and observed at low frequency in March (26%), but increased rapidly by April (65%) and May (70%) (Fig. 1b), indicating a transmission advantage over viruses with D614. Korber et al. noted that this change also correlated with increased viral loads in COVID-19 patients22, but because this change is also associated with the mutations in viral nsp3 and RdRp proteins, the role of the S-protein in these observations remained undefined. To determine if the D614G mutation alters the properties of the S-protein in a way that could impact transmission or replication, we assessed its role in viral entry. Maloney murine leukemia virus (MLV)-based pseudoviruses (PVs), expressing green fluorescent protein (GFP) and pseudotyped with the S protein of SARS-CoV-2 (SARS2) carrying the D614 or G614 genotype (SD614 and SG614, respectively) were produced from transfected HEK293T cells as previously described31. An SD614 variant, in which the furin-cleavage motif between the S1 and S2 domains is ablated (SFKO), was also included for comparison. HEK293T cells transduced to express human ACE2 (hACE2-293T) or those transduced with vector alone (Mock-293T) were infected with the same particle numbers of the PVs pseudotyped with the SD614, SG614, or SFKO (PVD614, PVG614, or PVFKO, respectively), and infection level was assessed one day later. We observed PVG614 infected hACE2-293T cells with approximately 9-fold higher efficiency than did PVD614 (Fig. 1c,d). This enhanced infectivity of PVG614 is not an artifact of PV titer normalization, as their titers are very similar (Extended Data Fig. 1). We next investigated the mechanism with which SG614 increased virus infectivity. Because S1 residue 614 is proximal to the S2 domain, we first compared the ratio between the S1 and S2 domains in the virion that might indicate altered release or shedding of the S1 domain after cleavage at the S1/S2 junction. To do so, we used S- protein constructs bearing Flag tags at both their amino (N)- and C-termini. PVs pseudotyped with these double-Flag tagged forms of SD614, SG614, and SFKO were partially purified and concentrated by pelleting through a 20% sucrose layer32 and evaluated for their infectivity. The titers of PVs were similar among PVG614, PVD614, and PVFKO before and after purification (Fig. 2a). In addition, modification by Flag-tags or pelleting of PVs through a sucrose layer did not alter the relative infectivity between PVG614 and PVD614 (Fig. 2b). We then determined their S1:S2 ratio by western blotting using the anti-Flag M2 antibody. As shown in Fig. 2c, the S1:S2 ratio is markedly greater in PVG614 compared to PVD614, indicating that glycine at residue 614 of SG614 stabilizes the interaction between the S1 and S2 domains, limiting S1 shedding. In addition, the total amount of the S protein in PVG614 is also much higher than that in PVD614, as indicated by a denser S2 band, even though the same number of pseudovirions was analyzed, as determined by quantitative PCR. To independently confirm that similar number of virions was analyzed, the lower part of the same membrane was blotted with an anti-p30 MLV gag antibody (Fig. 2c). Similar densities of p30 bands were observed from all PVs, indicating that differences in S-protein incorporation observed with PVG614 and PVD614 were due to the mutation of residue 614, not by different amount of PVs analyzed. A similar experiment performed with independently produced PVs yielded a nearly identical result (Extended Data Fig. 2a). Densitometric analysis shows there is 4.7 times more S1+S2 band in PVG614 compared to PVD614 (Fig. 2d). To more accurately estimate the S1:S2 ratio, we next compared different amount of the same samples so that S2-band intensity in PVG614 and PVD614 was comparable (Fig. 2e). Averages of several quantification show that the S1:S2 ratio of PVG614 is 3.5 times higher than that of PVD614 (Fig. 2f). The M2 antibody used in this experiment binds the Flag tag located at both the N- and C-termini of a protein, but it binds N-terminal Flag tag more efficiently33. Therefore, we directly visualized virion S-protein bands by silver staining (Extended Data Fig. 2b). Although the S2 bands are masked by a same-sized MLV protein, S1 bands are well separated. Again, the intensity of the S1 band of PVG614 is much stronger than that of PVD614, while p30 bands are comparable, a result consistent with those observed using the anti-Flag M2 antibody. We next confirmed these findings using virus-like particles (VLPs) composed only of the native SARS-CoV-2 proteins, the nucleoprotein (N), membrane protein (M), envelope protein (E), and S protein34. VLPs were partially purified and analyzed in the same way as MLV PVs. The S protein bands were detected with the anti-Flag M2 antibody, and the N protein with pooled convalescent plasma derived from COVID-19 patients. The S1:S2 ratio and total S protein on the virion was again much higher in the VLPs carrying SG614 (VLPG614) compared to those carrying SD614 (VLPD614) (Fig. 3a). The S1:S2 ratio is 3.4 fold higher and the total S protein is nearly five fold enriched in VLPG614 compared to VLPD614 (Fig. 3b,c). Thus, the D614G mutation enhances virus infection through two related mechanisms: It reduces S1 shedding and increases total S protein incorporated into the virion. It has previously been speculated that D614G mutation promotes an open configuration of the S protein that is more favorable to ACE2 association5,22,23,35. To explore this possibility, we investigated whether ACE2 binding by SG614 was more efficient than that by SD614. HEK293T cells transfected to express each S protein were assessed for their binding of hACE2 immunoadhesin, using hACE2-NN-Ig, whose enzymatic activity was abolished by mutation31. hACE2-NN-Ig bound SARS-CoV-1 RBD with an equivalent affinity as hACE2-Ig. These S proteins are fused to a C-terminal, but not an N-terminal Flag tag, thus allowing for the measurement of total S protein expression in permeabilized cells by flow cytometry. Although total S protein expression was comparable, hACE2-NN-Ig binding to the cells expressing SG614 was substantially higher than its binding to cells expressing SD614 (Fig. 4a). This observation has several explanations. First, G614 could increase hACE2 association by promoting greater exposure of the RBD, or second, this mutation could increase the number of binding sites by limiting S1-domain shedding. To differentiate these possibilities, we appended the Myc-tag to the N-terminus of the S-protein that is Flag tagged at its C-terminus and repeated the study, this time detecting the S1 domain with an anti-Myc antibody. As shown in Fig. 4b, the ratio of Myc-tag to Flag-tag is higher in cells expressing SG614 than in cells expressing SD614. However, the Myc-tag/Flag-tag ratio is similar to the hACE2- NN-Ig/Flag-tag ratio, indicating that increased hACE2 binding to the SG614-expressing cells did not result from increased affinity of SG614 spikes to hACE2 or greater access to the RBD. Instead, these data show there is more S1 domain in the SG614-expressing cells, a result again consistent with the observation that the D614G mutation reduces S1 shedding. We then assessed whether differential amount of the S protein could influence neutralization sensitivity of the virus. Fig. 4c shows that PVD614 and PVG614 are similarly susceptible to neutralizing antisera, indicating that antibody-mediated control of viruses carrying SD614 and SG614 would be similar. It has also been speculated that the D614G mutation would promote, not limit, shedding of the S1 domain, based on the hypothetical loss of a hydrogen bond between D614 in S1 and T859 in S222. An alternative explanation, more consistent with the data presented here, is that Q613 forms a hydrogen bond with T859, and the greater backbone flexibility provided by introduction of glycine at an adjacent position 614 enables a more favorable orientation of Q613. It is also possible that D614 can form an intra-domain salt bridge with R646, promoting a local S1 conformation unfavorable to its association with S2. In this model, replacing aspartic acid with glycine at the position 614 would prevent sampling of this unfavorable configuration. The instability of SD614 may also account for the observed lower level of incorporation of the functional S protein into PVs and VLPs. Specifically, the S-protein trimers with the exposed S2 domains, as a result of S1 shedding, could destabilize the trans-Golgi network membrane, the site of processing of the S1/S2 boundary, and such disruption may impede S-protein incorporation into the virion. In case of VLPs, this disruption would presumably further interfere with appropriate M- and N-protein associations by altering the conformation and orientation of the S-protein membrane-proximal regions. Alternatively, these S-protein trimers with the exposed S2 domains may serve as poor substrates for downstream post-translational modifications including palmitoylation, and those lacking proper modifications might be unsuitable for virion incorporation. An interesting question is why viruses carrying the more stable SG614 appear to be more transmissible without resulting in a major observable difference in disease severity22,27. It is possible that higher levels of functional S protein observed with SG614 increase the chance of host-to-host transmission, but that other factors limit the rate and efficiency of intra-host replication. Alternatively, the loss of virion-associated S proteins observed with SD614 may be compensated by greater fusion efficiency with the destabilized S protein when the next target cell is adjacent in a host tissue. It is also possible that our ability to detect sequence changes at this early stage of the pandemic is simply greater than our ability to detect modest differences in pathogenesis. The strong phenotypic difference we observe here between D614 and G614 suggests that more study on the impact of the D614G mutation on the course of disease is warranted. Finally, our data raise interesting questions about the natural history of SARS-CoV-2 as it moved presumably from horseshoe bats to humans. At some point in this process, the virus acquired a furin-cleavage site, allowing its S1/S2 boundary to be cleaved in virus- producing cells. In contrast, the S1/S2 boundary of SARS-CoV-1, and indeed all SARS- like viruses isolated from bats, lack this polybasic site and are cleaved by TMPRSS2 or endosomal cathepsins in the target cells13-20. Thus the greater stability we observe with SG614 would not be relevant to viruses lacking this site, but it appears to be strongly favored when a furin-cleavage site is present. Therefore, the D614G mutation may have emerged to compensate for this newly acquired furin site. In summary, we show that an S protein mutation that results in more transmissible SARS-CoV-2 also limits shedding of the S1 domain and increases S-protein incorporation into the virion. Further studies will be necessary to determine the impact of this change on the nature and severity of COVID-19. Acknowledgements This work is supported by an administrative supplement to NIH R01 AI129868 for coronavirus research (awarded to M.F. and H.C.) Author contributions H.C. and M.F. conceived of and supervised the study. L.Z., C.B.J., H.M., M.F., and H.C. designed experiments. L.Z., C.B.J., H.M., and A.O. performed experiments. L.Z., C.B.J., H.M., and A.O. analyzed data. E.B.R. and T.I. provided essential reagents. M.F. and H.C. wrote the manuscript. All authors reviewed and edited the manuscript. Competing interests The authors claim no competing interest. Data availability The data that support the findings of this study are available from the corresponding author upon reasonable request. Methods SARS-CoV-2 S protein sequences analysis. To track D614G variation among SARS- CoV-2 isolates, S protein sequences were downloaded from GenBank and separated by the month. Genotype frequency at residue 614 was calculated using R (R Foundation for Statistical Computing) with the Biostrings package. Logo plots of D614G variation were generated by WebLogo after sequence alignment. Total number of sequences analyzed for each month is indicated in the Fig. 1 legend. MLV PV production, quantification, and infection. MLV PVs were produced by transfecting HEK293T cells at ~60% confluency in T175 flasks using the calcium- phosphate method with 70 µg of total DNA. The ratio of 5:5:1 by mass was used for the retroviral vector pQCXIX encoding green fluorescence protein (GFP) or firefly luciferase (FLuc), a plasmid expressing MLV gag and pol proteins, and a plasmid expressing the S protein of SARS-CoV-2 or VSV G protein. The plasmid expressing VSV G protein was previously reported31. SARS-CoV-2 S protein gene used in the production of MLV PVs was codon-optimized and synthesized by Integrated DNA Technologies based on the protein sequence (GenBank YP_009724390). The S protein gene is fused to the Flag tag sequence (DYKDDDDK) either at its C-terminus or at both the N- and C-termini, as indicated in each experiment. PV-containing culture supernatants were collected at 43 h post transfection, cleared through 0.45 µm filters, and either purified or aliquoted and frozen at -80°C immediately. PV titers were quantified by RT-qPCR, using primers and a probe that target the CMV promoter. Sense primer: 5’-TCACGGGGATTTCCAAGTCTC-3’, anti-sense primer: 5’-AATGGGGCGGAGTTGTTACGAC-3’, probe: 5’- AAACAAACTCCCATTGACGTCA-3’. Viral RNA was extracted with Trizol (Life Technologies) and GlycoBlue coprecipitant (Invitrogen) and reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qPCR was performed using Luna Universal Probe qPCR Master Mix (New England Biolabs) with the known quantity of pQCXIX vector to generate standard curves. Infection assays were performed by spinoculating (at 2,100 x g for 30 min at 10°C) PVs onto the Mock- and hACE2-293T cells seeded on multiwell plates. Spinoculated plates were incubated for 2 h in a CO2 incubator and medium was replaced with DMEM containing 10% FBS. Infection levels were assessed 24 h post infection by measuring GFP expression by Accuri flow cytometer or luciferase activity using the Luc-Pair Firefly Luciferase HS Assay Kit (GeneCopoeia). Analyses of the S protein incorporated into MLV PV. For the analyses of the S protein in the virion, PVs were partially purified. 9 ml of cleared culture supernatants containing PVs (20 ml/T175) were loaded onto 2 ml of 20% sucrose in PBS and centrifuged at 30,000 rpm in the SW41 rotor for 2 h at 10°C32. PV pellets were resuspended in 30-50 µl NT buffer (120 mM NaCl, 20 mM Tris, pH8.0). Purified PVs were immediately used or aliquoted and frozen at -80°C. For western blot analyses, 5-10 µl of purified PV, which is equivalent to 0.5-1.0 x 1010 vector genomes, was loaded per lane of the 4-12% Bis-Tris gel (Life Technologies), transferred to the PVDF membrane, and blotted with 1 µg/ml anti-Flag M2 antibody (Sigma-Aldrich, F1804) to detect the S-protein bands. 1 µg/ml anti-p30 MLV gag antibody (Abcam, ab130757) and 1:10,000 dilution of goat-anti- mouse IgG-HRP polyclonal antibody (Jackson ImmunoResearch, 115-036-062) were used to detect MLV gag protein as an internal control. Band intensities were measured, using Image Lab software (Bio-Rad). To increase the accuracy of this measurement, the same blots were analyzed several times at different exposures. For silver staining, 30 µl of purified PVs were separated by the 4-12% Bis-Tris gel and stained with Silver Stain Plus (Bio-Rad). VLP production and S-protein analysis. SARS-CoV-2 VLPs were produced by transfecting HEK293T cells at ~60% confluency in T75 flasks with 25 µg total DNA using the calcium phosphate method as previously described with a modification34. The plasmids expressing SARS-CoV-2 M, N, E, and S proteins were transfected at a ratio of 1:5:5:1. The codon-optimized M, N, and E protein genes were synthesized based on the GenBank protein sequences, YP_009724393, YP_009724397, and YP_009724392, respectively. VLPs were harvested 43 h post transfection from the culture supernatants, cleared by 0.45 µm filtration, and partially purified by pelleting through a 20% sucrose layer as were MLV PVs. VLP pellets were resuspended in 30 µl of NT buffer, and the entire amount was loaded on the 4-12% Bis-Tris gel for WB analyses. As with MLV PV, the S-protein bands were visualized using the anti-Flag M2 antibody, and the N-protein band was detected using pooled convalescent plasma at a 1:500 dilution and 10 ng/ml goat-anti-human IgG antibody conjugated with polymerized HRP (Fitzgerald, 61R- I166AHRP40). Cell-surface expression and analysis of the S protein. HEK293T cells, approximately 80% confluent in 6-well plates were transfected with 8 µl PEI 40,000 (Polysciences) and 2 µg plasmid expressing the indicated S protein variant. For ACE2-NN-Ig binding experiments, the S protein constructs with the Flag tag only at the C-terminus, and for the measurement of total S1, those with N-terminal Myc tag (EQKLISEEDL) and C-terminal Flag tag (DYKDDDDK) are used. All tags are fused with a two-glycine (GG) linker. To measure ACE2-binding ability or the level of the S1 domain present on the cell surface, cells were detached two days post transfection with Accutase (Stemcell Technologies Inc.) and incubated with either 1 µg/ml purified hACE2-NN-Ig or anti-Myc antibody (clone 9E10, National Cell Culture Center, Minneapolis, MN), respectively, on ice. hACE2-NN-Ig was previous described and was purified using Protein A-Sepharose CL- 4B (GE Healthcare)31. To measure the total level of S protein, cells were permeabilized with PBS including 0.5% Triton X-100 (Sigma-Aldrich) at room temperature for 10 min and incubated with 1 µg/ml anti-Flag M2 antibody (Sigma-Aldrich). Neutralization assay with human immune plasma. Deidentified blood samples were obtained by the Allergy, Asthma and Immunology Specialists of South Florida, LLC for COVID-­‐19 serotyping, and exempt from human subject research under 45 CFR 45.101(b)(4). MLV PVs encoding firefly luciferase and pseudotyped with the indicated SARS-CoV-2 S protein were preincubated for 1 h at 37°C with or without convalescent or healthy plasma serially diluted in DMEM containing 10% FBS. Mock- and hACE2- HEK293T cells on 96-well plates were infected with the preincubation mixes and infection levels were assessed 24 h later by measuring luciferase activity using the Luc- Pair Firefly Luciferase HS Assay Kit (GeneCopoeia). Reagent availability. Plasmids and cell lines used in this study are available from the corresponding author upon request. Statistical analysis. All appropriate data were analyzed with GraphPad Prism 7 (GraphPad Software Inc.). All hypothesis tests were performed as two-tailed tests. Specific statistical analysis methods are described in the figure legends where results are presented. Values were considered statistically significant for p values below 0.05. Exact p values are provided in appropriate figures. https://www.scripps.edu/news-and-events/press-room/2020/20200611-choe-farzan-sars-cov-2-spike-protein.html

Mutated coronavirus shows significant boost in infectivity COVID-19-causing viral variant taking over in the United States and Europe now carries more functional, cell-binding spikes. June 12, 2020 Additional Resources Access the full study Video: Addressing the Coronavirus Challenge Learn more about coronavirus work at Scripps Research JUPITER, FL — A tiny genetic mutation in the SARS coronavirus 2 variant circulating throughout Europe and the United States significantly increases the virus’ ability to infect cells, lab experiments performed at Scripps Research show. “Viruses with this mutation were much more infectious than those without the mutation in the cell culture system we used,” says Scripps Research virologist Hyeryun Choe, PhD, senior author of the study. The mutation had the effect of markedly increasing the number of functional spikes on the viral surface, she adds. Those spikes are what allow the virus to bind to and infect cells. “The number—or density—of functional spikes on the virus is 4 or 5 times greater due to this mutation,” Choe says. The spikes give the coronavirus its crown-like appearance and enable it to latch onto target cell receptors called ACE2. The mutation, called D614G, provides greater flexibility to the spike’s “backbone,” explains co-author Michael Farzan, PhD, co-chairman of the Scripps Research Department of Immunology and Microbiology. More flexible spikes allow newly made viral particles to navigate the journey from producer cell to target cell fully intact, with less tendency to fall apart prematurely, he explains. “Our data are very clear, the virus becomes much more stable with the mutation,” Choe says. There has been much debate about why COVID-19 outbreaks in Italy and New York have so quickly overwhelmed health systems, while early outbreaks in places like San Francisco and Washington state proved more readily managed, at least initially. Was it something about those communities and their response, or had the virus somehow changed? All viruses acquire minute genetic changes as they reproduce and spread. Those changes rarely impact fitness or ability to compete. The SARS-CoV-2 variant that circulated in the earliest regional outbreaks lacked the D614G mutation now dominating in much of the world. But was that because of the so-called “founder effect,” seen when a small number of variants fan out into a wide population, by chance? Choe and Farzan believe their biochemical experiments settle the question. “There have been at least a dozen scientific papers talking about the predominance of this mutation,” Farzan says. “Are we just seeing a ‘founder effect?’ Our data nails it. It is not the founder effect.” Choe and Farzan’s paper is titled “The D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases infectivity.” Now undergoing peer review, it is being posted prior to publication to the pre-print site bioRxiv, and released early, amid news reports of its findings. Choe and Farzan note that their research was performed using harmless viruses engineered to produce key coronavirus proteins. Whether the changes they observed also translate to increased transmissibility in the real world requires additional epidemiological studies, they note. Encouragingly, the duo found that immune factors from the serum of infected people work equally well against engineered viruses both with and without the D614G mutation. That’s a hopeful sign that vaccine candidates in development will work against variants with or without that mutation, Choe says. Choe and Farzan have studied coronaviruses for nearly 20 years, since the first outbreak of SARS, a similar virus. They were the first to discover in 2003 that SARS bound to the ACE2 receptor on cells. Others’ experiments have shown the SARS-CoV-2 virus binds the same ACE2 receptor. But Farzan and Choe note a key structural difference between spike proteins on the first SARS virus and this new pandemic strain. With both, under an electron microscope, the spike has tripod shape, with its three segments bound together at a backbone-like scaffold. But SARS-CoV-2 is different. Its tripod is divided in two discreet segments, S1 and S2. Initially, this unusual feature produced unstable spikes, Farzan says. Only about a quarter of the hundreds of spikes on each SARS-CoV-2 virus maintain the structure they need to successfully infect a target cell. With the mutation, the tripod breaks much less frequently, meaning more of its spikes are fully functional, he says. The addition of the D614G mutation means that the amino acid at that location is switched from aspartic acid to glycine. That renders it more bendable, Farzan says. Evidence of its success can be seen in the sequenced strains that scientists globally are contributing to databases including GenBank, the duo reports. In February, no sequences deposited to the GenBank database showed the D614G mutation. But by March, it appeared in 1 out of 4 samples. In May, it appeared in 70 percent of samples, Farzan says. “Over time, it has figured out how to hold on better and not fall apart until it needs to,” Farzan says. “The virus has, under selection pressure, made itself more stable.” It is still unknown whether this small mutation affects the severity of symptoms of infected people, or increases mortality, the scientists say. While ICU data from New York and elsewhere reports a preponderance of the new D614G variant, much more data, ideally under controlled studies, are needed, Choe says. In addition to senior authors Choe and Farzan, the authors include first authors Lizhou Zhang, Cody Jackson and Huihui Mou, plus co-authors Amrita Ojha, Erumbi Rangarajan and Tina Izard, all of Scripps Research. The work was supported by the National Institutes of Health through an administrative supplement to RO1 AI129868 for coronavirus research. https://www.scripps.edu/news-and-events/press-room/2020/20200612-choe-farzan-coronavirus-spike-mutation.html

Total Cases 676 Cumulative (includes recovered cases) https://coronavirus-response-alaska-dhss.hub.arcgis.com/ Total Recovered Cases 429 Total Hospitalizations 54 Cumulative (does not reflect current stays) Total Deaths 12

https://health.wyo.gov/publichealth/infectious-disease-epidemiology-unit/disease/novel-coronavirus/covid-19-map-and-statistics/

https://montana.maps.arcgis.com/apps/MapSeries/index.html?appid=7c34f3412536439491adcc2103421d4b

https://health.hawaii.gov/coronavirusdisease2019/what-you-should-know/current-situation-in-hawaii/

https://www.health.nd.gov/diseases-conditions/coronavirus/north-dakota-coronavirus-cases

Total Cases 1,131 3 New Currently Hospitalized 2 Hospitalized Under Investigation 10 Total People Recovered 914 Deaths 55 People Tested 52,890 People Being Monitored 576 People Completed Monitoring 1,059 https://www.healthvermont.gov/response/coronavirus-covid-19/current-activity-vermont

Updated: June 16, 2020 at 12:00 PM Total Cases1 Confirmed Cases Probable Cases Recovered Hospitalizations Deaths 2,819 2,499 320 2,233 321 101 1Maine's total case count includes both confirmed and probable cases. For more information about this data, please see the "Read Details About the Data" section below. https://www.maine.gov/dhhs/mecdc/infectious-disease/epi/airborne/coronavirus.shtml View a Table of All Reported COVID-19 Tests in Maine All Reported COVID-19 Tests in Maine Result Antibody Molecular Testing Total Positive 290 3,441 3,731 Negative 5,715 69,516 75,231 Indeterminate 8 114 122 Total 6,013 73,071 79,084 Results from Labs Reporting Electronically (Used for Percent Positivity Calculations)* Result Antibody Molecular Testing Total Positive 215 3,246 3,461 Negative 5,712 69,500 75,212 Indeterminate 8 113 121 Total 5,935 72,859 78,794 Percent Positive 3.62 4.46 4.39 *Not all labs report results to the Maine CDC electronically. Labs reporting manually report only the positive results and are therefore excluded for purposes of calculating the percent positivity rate. Molecular testing includes PCR, isothermal, and NAAT methods. Updated June 16, 2020 at 12:00 PM View a Table of Maine COVID-19 Current Hospital Use and Capacity Data View a Table of Cumulative COVID‑19 Cases by County

https://dhhr.wv.gov/COVID-19/Pages/default.aspx

New Hampshire 2019 Novel Coronavirus (COVID-19) Summary Report (data updated as of June 16, 2020, 9:00 AM) https://www.nh.gov/covid19/ Number of Persons with COVID-19 1 5,364 Recovered 4,067 (76%) Deaths Attributed to COVID-19 326 (6%) Total Current COVID-19 Cases 971 Persons Who Have Been Hospitalized for COVID-19 521 (10%) Current Hospitalizations 62 Total Persons Tested at Selected Laboratories, Polymerase Chain Reaction (PCR)2 100,086 Total Persons Tested at Selected Laboratories, Antibody Laboratory Tests2 16,285 Persons with Specimens Submitted to NH PHL 30,791 Persons with Test Pending at NH PHL3 304 Persons Being Monitored in NH (approximate point in time) 3,600 1 Includes specimens positive at any laboratory and those confirmed by CDC confirmatory testing.2 Includes specimens tested at the NH Public Health Laboratories (PHL), LabCorp, Quest, Dartmouth-Hitchcock Medical Center, and those sent to CDC prior to NH PHL testing capacity.3 Includes specimens received and awaiting testing at NH PHL. Does not include tests pending at commercial laboratories. Active Cases Dashboard | Active Cases Map

1,328 Douglas 268 Lancaster 236 Sarpy 150 Dodge 62 Scotts Bluff 50 Dakota 44 Hall 41 Platte 25 Colfax 19 Thurston 17 Saline 17 Saunders 16 Madison 10 Dixon 10 Buffalo 9 Washington 8 Dawson 8 Wayne 7 Cass 6 Adams 6 Otoe 6 Lincoln 6 Fillmore 4 Seward 4 Merrick 3 Burt 3 Custer 3 Butler 3 Knox 3 Valley 2 Cheyenne 2 Garden 2 Howard 2 Jefferson 1 Polk 1 Garfield 1 Boone 1 Box Butte 1 Kearney 1 Webster 1 Banner 1 Greeley 1 Sioux 1 Sherman 1 Red Willow 1 Stanton 1 Morrill 1 Hamilton https://nebraska.maps.arcgis.com/apps/opsdashboard/index.html#/4213f719a45647bc873ffb58783ffef3

https://www.doh.wa.gov/Emergencies/NovelCoronavirusOutbreak2020COVID19/DataDashboard

https://doh.sd.gov/news/Coronavirus.aspx

https://coronavirus.idaho.gov/

Arkansas (8 Cases) Ashley (47 Cases) Baxter (14 Cases) Benton (1,514 Cases) Boone (10 Cases) Bradley (35 Cases) Calhoun (0 Cases) Carroll (92 Cases) Chicot (58 Cases) Clark (48 Cases) Clay (22 Cases) Cleburne (75 Cases) Cleveland (20 Cases) Columbia (29 Cases) Conway (24 Cases) Craighead (365 Cases) Crawford (65 Cases) Crittenden (545 Cases) Cross (79 Cases) Dallas (4 Cases) Desha (23 Cases) Drew (26 Cases) Faulkner (180 Cases) Franklin (9 Cases) Fulton (9 Cases) Garland (165 Cases) Grant (24 Cases) Greene (74 Cases) Hempstead (40 Cases) Hot Spring (43 Cases) Howard (42 Cases) Independence (43 Cases) Izard (20 Cases) Jackson (16 Cases) Jefferson (633 Cases) Johnson (93 Cases) Lafayette (11 Cases) Lawrence (91 Cases) Lee (545 Cases) Lincoln (995 Cases) Little River (34 Cases) Logan (20 Cases) Lonoke (75 Cases) Madison (122 Cases) Marion (2 Cases) Miller (73 Cases) Missing County Info (396 Cases) Mississippi (98 Cases) Monroe (7 Cases) Montgomery (2 Cases) Nevada (83 Cases) Newton (3 Cases) Ouachita (16 Cases) Perry (16 Cases) Phillips (52 Cases) Pike (7 Cases) Poinsett (51 Cases) Polk (58 Cases) Pope (285 Cases) Prairie (9 Cases) Pulaski (1,343 Cases) Randolph (29 Cases) Saline (184 Cases) Scott (5 Cases) Searcy (7 Cases) Sebastian (169 Cases) Sevier (478 Cases) Sharp (50 Cases) St. Francis (779 Cases) Stone (11 Cases) Union (209 Cases) Van Buren (30 Cases) Washington (1,977 Cases) White (61 Cases) Woodruff (5 Cases) Yell (309 Cases) https://experience.arcgis.com/experience/c2ef4a4fcbe5458fbf2e48a21e4fece9

Data current as of 6/16/2020, 12:01 a.m. Updated Monday - Friday.* Total cases 6,0981 Total deaths 182 Positive tests 5,897 Negative tests 173,440 Total tested 179,337 * For daily counts of cases, deaths and negative tests on weekends, please see our weekend press releases available here. 1Includes cases confirmed by diagnostic testing and presumptive cases. Presumptive cases are those without a positive diagnostic test who present COVID-19-like symptoms and had close contact with a confirmed case. https://govstatus.egov.com/OR-OHA-COVID-19

Positive 9,933 Total Tests * 271,553 Click Here to View Positive Cases By County COVID-Related Deaths in NM 447 *Numbers are cumulative persons tested through 6/16/2020, 5:51:32 PM. Test results are from the state Scientific Laboratory Division of the New Mexico Department of Health, TriCore Reference Laboratories, LabCorp, Mayo Clinic Laboratories, Quest Diagnostics, and BioReference Laboratories. https://cv.nmhealth.org/

Aitkin County: 13 Anoka County: 1,770 Becker County: 50 Beltrami County: 23 Benton County: 193 Big Stone County: 13 Blue Earth County: 164 Brown County: 20 Carlton County: 80 Carver County: 276 Cass County: 11 Chippewa County: 57 Chisago County: 80 Clay County: 506 Clearwater County: 5 Cook County: 1 Cottonwood County: 111 Crow Wing County: 90 Dakota County: 1,758 Dodge County: 59 Douglas County: 48 Faribault County: 28 Fillmore County: 20 Freeborn County: 222 Goodhue County: 94 Grant County: 6 Hennepin County: 10,323 Houston County: 5 Hubbard County: 5 Isanti County: 50 Itasca County: 59 Jackson County: 48 Kanabec County: 13 Kandiyohi County: 548 Kittson County: 1 Koochiching County: 9 Lac Qui Parle County: 3 Lake County: 2 Le Sueur County: 52 Lincoln County: 6 Lyon County: 222 Mahnomen County: 6 Marshall County: 12 Martin County: 147 McLeod County: 54 Meeker County: 50 Mille Lacs County: 28 Morrison County: 54 Mower County: 661 Murray County: 43 Nicollet County: 95 Nobles County: 1,620 Norman County: 17 Olmsted County: 781 Otter Tail County: 83 Pennington County: 51 Pine County: 96 Pipestone County: 14 Polk County: 67 Pope County: 10 Ramsey County: 4,033 Red Lake County: 4 Redwood County: 12 Renville County: 12 Rice County: 697 Rock County: 25 Roseau County: 6 Scott County: 541 Sherburne County: 270 Sibley County: 25 St. Louis County: 124 Stearns County: 2,109 Steele County: 175 Stevens County: 1 Swift County: 20 Todd County: 375 Traverse County: 5 Wabasha County: 21 Wadena County: 10 Waseca County: 34 Washington County: 802 Watonwan County: 99 Wilkin County: 15 Winona County: 85 Wright County: 383 Yellow Medicine County: 11 https://mndps.maps.arcgis.com/apps/opsdashboard/index.html#/f28f84968c1148129932c3bebb1d3a1a