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Earliest Full Zika Sequence In Americas Is From Haiti Not Brazil


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Posted (edited)

The recent Science paper of sequences in the Americas including those in Brazil noted that a timeline based on the 20 sequences in Figure 3 suggested that Zika arrived in the Americas in 2013.  The early cases as well as the number of cases in Brazil suggested that this early entry was in Brazil.  However, the earliest sequence reported for the Americas (excluding those from Chile owned Easter Islands) was a Dec 12, 2014 collect from Haiti, 3 months prior to the earliest sequence from Brazil (Mar 14, 2015 for the 20 sequences in Fig 3).

The sequences in the Americas are closely related to those from the 2013 outbreak in French Polynesia.  Although sequences were generated for residents in French Polynesia, including a sequence from Marquesas Islands, almost 1000 miles northeast of Tahiti, these sequences were short (in the range of 200-400 BP and were excluded from fig 3, which used sequences greater than 1500 BP).  Similarly, most of the sequences from French Polynesia were from travelers from New Caledonia, Japan, Norway, and France, which were also short.  The only full sequence, H/PF/2013, was from a 51 year old female diagnosed in metropolitan France (labeled as KJ776791 French Polynesia in fig 3).

See sequence map

https://www.google.com/maps/d/u/0/edit?mid=zv94AJqgUct4.kI8kcFySb4J0&hl=en

The 20 sequences from the Americas are most closely related to this sequence.  However, several of the sequences from Suriname are included, and the shorter sequences are between 1900-2600 BP, indicating that the “B” branch (the sequences from the Americas) is defined by a single nucleotide change (T2547C of the coding sequence).

Sequences covering this region are limited.  None of the shorter French Polynesia sequences cover position 2544, which is also true of subsequent sequences from Zika outbreaks in New Caledonia and Easter Island.  There were a limited number of short sequences associated with local transmission in New Caledonia, but there are 51 sequences from Easter Island (2014 collections).  However, these sequences are all under 1000 BP and cover the NS5 region at the 3’ end of the 10,272 BP protein coding region, which also does not overlap position 2547.

Thus, the only 2013/2014 sequence from French Polynesia, New Caledonia, or Easter Island covering position 2544 is the H/PF/2013 sequence.  Therefore, the possibility that T2547C was present on these sequences, collected prior to sequences from the Americas, cannot be excluded, so the earlier evolution could have been in these countries, or any other country in the South Pacific or the Americas.

In fact, the earliest sequence from the Americas is from Haiti (labeled as KU509998 Haiti) in Fig 3.  It is the sequence with the earliest collection date (Dec 12, 2014) and is a precursor for the earliest sequence from Brazil (labeled KU321639 Brazil) which was collected on Mar 14, 2015 and is on the same branch as the Haiti sequence.

Thus, more information on this case (local or travel from some other country), would aid in determining the location of the first “B” group sequence in the Americas, as would full sequences from 2013/2014 collections from French Polynesia, New Caledonia, or Eastern Island.

 

 

Edited by niman
Posted
LOCUS       KU509998               10676 bp    RNA     linear   VRL 02-FEB-2016
DEFINITION  Zika virus strain Haiti/1225/2014, complete genome.
ACCESSION   KU509998
VERSION     KU509998.1  GI:983657312
KEYWORDS    .
SOURCE      Zika virus
  ORGANISM  Zika virus
            Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA
            stage; Flaviviridae; Flavivirus.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Lednicky,J.A., Morris,J.G. Jr., Beau De Rochars,V.M., Elbadry,M.A.,
            Okech,B.A. and Loeb,J.C.
  TITLE     Zika virus from a Haitian, December 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Lednicky,J.A., Morris,J.G. Jr., Beau De Rochars,V.M., Elbadry,M.A.,
            Okech,B.A. and Loeb,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2016) Environmental and Global Health, University
            of Florida - Gainesville, 1225 Center Drive, Room 4155,
            Gainesville, FL 32610, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Zika virus"
                     /mol_type="genomic RNA"
                     /strain="Haiti/1225/2014"
                     /isolation_source="plasma"
                     /host="Homo sapiens"
                     /db_xref="taxon:64320"
                     /country="Haiti"
                     /collection_date="12-Dec-2014"
                     /note="genotype: Asian"

http://www.ncbi.nlm.nih.gov/nuccore/KU509998.1

 

Posted
Sequences producing significant alignments:

Select:AllNone Selected:0

Sequences producing significant alignments:
Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccession
1852518525100%0.0100%KU509998.1
1850218502100%0.099%KU321639.1
1844718447100%0.099%KJ776791.1
1842018420100%0.099%KU729218.1
1842018420100%0.099%KU707826.1
1842018420100%0.099%KU365779.1
1840818408100%0.099%KU926309.1
1840818408100%0.099%KU365780.1
1840218402100%0.099%KU940228.1
1840218402100%0.099%KU527068.1
1840218402100%0.099%KU647676.1
1840218402100%0.099%KU365777.1
1839918399100%0.099%KU501217.1
183951839599%0.099%KU497555.1
1839318393100%0.099%KU729217.2
1839318393100%0.099%KU501216.1
1839018390100%0.099%KU820897.1
1839018390100%0.099%KU365778.1
1839018390100%0.099%KU312312.1
1838418384100%0.099%KU922960.1
1838118381100%0.099%KU926310.1
1838118381100%0.099%KU922923.1
1838118381100%0.099%KU501215.1
1837518375100%0.099%KU870645.1
1837218372100%0.099%KU853013.1
1837018370100%0.099%KU853012.1
1836318363100%0.099%KU820898.1
1835718357100%0.099%KU955590.1
1835418354100%0.099%KU740184.2
1835418354100%0.099%KU761564.1
1834518345100%0.099%KU955589.1
1834518345100%0.099%KU820899.2
183091830999%0.099%KU940224.1
1825818258100%0.099%KU744693.1
1823618236100%0.099%KU866423.1
1810518105100%0.099%KU681081.3
1779817798100%0.098%KU955593.1
177981779899%0.098%JN860885.1
177511775198%0.099%KF993678.1
1764317643100%0.098%EU545988.1
1748817488100%0.098%KU681082.3
164661646699%0.096%HQ234499.1
1328413284100%0.089%KF268949.1
1328413284100%0.089%KF268948.1
1328113281100%0.089%KU720415.1
1327913279100%0.089%KF383115.1
1327713277100%0.089%KF268950.1
132751327599%0.089%HQ234498.1
1327213272100%0.089%KU955595.1
1326613266100%0.089%DQ859059.1
1326313263100%0.089%KU955592.1
1326313263100%0.089%KF383119.1
1325913259100%0.089%LC002520.1
1324513245100%0.089%KU955591.1
1324313243100%0.089%KU955594.1
1322113221100%0.089%KF383116.1
132141321499%0.089%HQ234501.1
1320013200100%0.089%AY632535.2
1314213142100%0.088%KF383117.1
131351313599%0.088%HQ234500.1
1294013008100%0.088%KF383118.1
128611286197%0.089%KF383121.1
108441084497%0.084%KF383120.1
50651448087%0.095%KU940227.1
4985498527%0.099%KU312314.1
4958495827%0.099%KU312313.1
4664466425%0.099%KU646828.1
4655465525%0.099%KU646827.1
3443344318%0.099%KU312315.1
3220322017%0.099%KU740199.1
2879419795%0.071%DQ859064.1
2700270014%0.099%KJ634273.1
2057205711%0.099%KU686218.1
2030203011%0.099%KU179098.1
176117619%0.0100%KM078936.1
175717579%0.099%KM078961.1
175517559%0.099%KM078930.1
175417549%0.099%KM078971.1
175417549%0.099%KM078970.1
175417549%0.099%KM078933.1
175217529%0.099%KM078929.1
161116118%0.0100%KJ873160.1
142914297%0.0100%KJ873161.1
139113917%0.099%KM851039.1
135513557%0.098%KM851038.1
134613467%0.099%KU556802.1
1306130610%0.088%AF013415.1
124512456%0.099%KT200609.1
124012406%0.099%KU232300.1
123112316%0.099%KU232290.1
122912296%0.099%KU232297.1
122012206%0.099%KU232294.1
121812186%0.099%KU232292.1
121412146%0.099%KU232298.1
121112116%0.099%KU232296.1
121112116%0.099%KU232293.1
120512056%0.099%KU232295.1
119511956%0.099%KU232288.1
119111916%0.099%KU232289.1
118711876%0.099%KU232299.1
118611866%0.099%KU232291.1
113311336%0.099%KU758878.1
107710778%0.089%KF270886.1
102010208%0.087%AF372422.1
101810185%0.0100%KU867812.1
9969967%0.089%KF270887.1
9809807%0.089%KF383022.1
980198664%0.070%EF158064.1
9759757%0.089%KF383026.1
9679677%0.088%EU303241.1
9669667%0.088%KF383032.1
9629627%0.088%KF383034.1
9629627%0.088%KF383029.1
9609607%0.088%KF383037.1
9579577%0.088%KF383036.1
9579577%0.088%KF383033.1
9399397%0.088%KF383039.1
9399397%0.088%KF383028.1
9359357%0.088%KF383031.1
9309307%0.087%KF383038.1
9219217%0.087%KF383046.1
915204758%0.069%EU566860.1
913205064%0.069%KT823415.1
913202564%0.069%FJ753286.2
9069067%0.087%KF383045.1
9029027%0.087%KF383043.1
9019017%0.086%KF383035.1
8978976%0.088%KF383103.1
8978976%0.088%KF383086.1
8978977%0.087%KF383041.1
8938937%0.086%KF383042.1
890196267%0.069%EF158069.1
8668667%0.086%KF383040.1
8658656%0.087%KF383104.1
8658656%0.087%KF383085.1
8618616%0.087%KF383106.1
8568566%0.087%KF383114.1
8568566%0.087%KF383107.1
8568566%0.087%KF383088.1
8528526%0.087%KF383087.1
8388386%0.086%KF383089.1
8388384%0.099%AB908162.1
8348346%0.086%KF383101.1
8348346%0.086%KF383097.1
8298296%0.086%KF383099.1
8258256%0.086%KF383098.1
8148144%0.099%KU872850.1
Posted

Phylogenetic tree of full Zika sequences at Genbank with Haiti sequence labeled Haiti/1225/2014, ealiiest Brazil sequence labeled ZikaSPH2015, and French Polynesia sequence labeled H/PF/2013_French_Polynesia

Posted

A report on the outbreak of Zika virus on Easter Island, South Pacific, 2014.

Tognarelli,J., Ulloa,S., Villagra,E., Lagos,J., Aguayo,C., Fasce,R., Parra,B., Mora,J., Becerra,N., Lagos,N., Vera,L., Olivares,B., Vilches,M. and Fernandez,J.

(11-26-2015) Arch. Virol.

PMID:
 
26611910
 
Citation

Sequences in this data set

  • KM078979.1Zika virus strain CHI2909014 NS5 protein gene, partial cds
  • KM078978.1Zika virus strain CHI2908914 NS5 protein gene, partial cds
  • KM078977.1Zika virus strain CHI2908714 NS5 protein gene, partial cds
  • KM078976.1Zika virus strain CHI2871014 NS5 protein gene, partial cds
  • KM078975.1Zika virus strain CHI2759614 NS5 protein gene, partial cds
  • KM078974.1Zika virus strain CHI2759514 NS5 protein gene, partial cds
  • KM078973.1Zika virus strain CHI2759014 NS5 protein gene, partial cds
  • KM078972.1Zika virus strain CHI2758514 NS5 protein gene, partial cds
  • KM078971.1Zika virus strain CHI2613014 NS5 protein gene, partial cds
  • KM078970.1Zika virus strain CHI2490414 NS5 protein gene, partial cds
  • KM078969.1Zika virus strain CHI2488314 NS5 protein gene, partial cds
  • KM078968.1Zika virus strain CHI2488214 NS5 protein gene, partial cds
  • KM078967.1Zika virus strain CHI2487714 NS5 protein gene, partial cds
  • KM078966.1Zika virus strain CHI2348014 NS5 protein gene, partial cds
  • KM078965.1Zika virus strain CHI2759214 NS5 protein gene, partial cds
  • KM078964.1Zika virus strain CHI2758914 NS5 protein gene, partial cds
  • KM078963.1Zika virus strain CHI2758614 NS5 protein gene, partial cds
  • KM078962.1Zika virus strain CHI2612714 NS5 protein gene, partial cds
  • KM078961.1Zika virus strain CHI2612114 NS5 protein gene, partial cds
  • KM078960.1Zika virus strain CHI2611714 NS5 protein gene, partial cds
  • KM078959.1Zika virus strain CHI2611514 NS5 protein gene, partial cds
  • KM078958.1Zika virus strain CHI2611214 NS5 protein gene, partial cds
  • KM078957.1Zika virus strain CHI2489814 NS5 protein gene, partial cds
  • KM078956.1Zika virus strain CHI2487814 NS5 protein gene, partial cds
  • KM078955.1Zika virus strain CHI2487414 NS5 protein gene, partial cds
  • KM078954.1Zika virus strain CHI2487314 NS5 protein gene, partial cds
  • KM078953.1Zika virus strain CHI2347814 NS5 protein gene, partial cds
  • KM078952.1Zika virus strain CHI2347614 NS5 protein gene, partial cds
  • KM078951.1Zika virus strain CHI2347414 NS5 protein gene, partial cds
  • KM078950.1Zika virus strain CHI2347214 NS5 protein gene, partial cds
  • KM078949.1Zika virus strain CHI2283914 NS5 protein gene, partial cds
  • KM078948.1Zika virus strain CHI2283814 NS5 protein gene, partial cds
  • KM078947.1Zika virus strain CHI2283514 NS5 protein gene, partial cds
  • KM078946.1Zika virus strain CHI2283014 NS5 protein gene, partial cds
  • KM078945.1Zika virus strain CHI2091414 NS5 protein gene, partial cds
  • KM078944.1Zika virus strain CHI2091114 NS5 protein gene, partial cds
  • KM078943.1Zika virus strain CHI2282814 NS5 protein gene, partial cds
  • KM078942.1Zika virus strain CHI1805114 NS5 protein gene, partial cds
  • KM078941.1Zika virus strain CHI1805014 NS5 protein gene, partial cds
  • KM078940.1Zika virus strain CHI1757714 NS5 protein gene, partial cds
  • KM078939.1Zika virus strain CHI1805314 NS5 protein gene, partial cds
  • KM078938.1Zika virus strain CHI1472814 NS5 protein gene, partial cds
  • KM078937.1Zika virus strain CHI1410414 NS5 protein gene, partial cds
  • KM078936.1Zika virus strain CHI1410214 NS5 protein gene, partial cds
  • KM078935.1Zika virus strain CHI1379614 NS5 protein gene, partial cds
  • KM078934.1Zika virus strain CHI1379514 NS5 protein gene, partial cds
  • KM078933.1Zika virus strain CHI1058514 NS5 protein gene, partial cds
  • KM078932.1Zika virus strain CHI1131214 NS5 protein gene, partial cds
  • KM078931.1Zika virus strain CHI2908814 NS5 protein gene, partial cds
  • KM078930.1Zika virus strain CHI2283714 NS5 protein gene, partial cds
  • KM078929.1Zika virus strain CHI1805214 NS5 protein gene, partial cds
  • 3 weeks later...
Posted
LOCUS       KX051563               10807 bp    cRNA    linear   VRL 12-APR-2016
DEFINITION  Zika virus isolate Haiti/1/2016, complete genome.
ACCESSION   KX051563
VERSION     KX051563.1  GI:1016884664
KEYWORDS    .
SOURCE      Zika virus
  ORGANISM  Zika virus
            Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA
            stage; Flaviviridae; Flavivirus.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Lednicky,J.A., White,S.K., Loeb,J.C., Iovine,N.M. and Morris,J.G.
            Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2016) Environmental and Global Health, University
            of Florida - Gainesville, 1225 Center Drive, Room 4155,
            Gainesville, FL 32610, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Zika virus"
                     /mol_type="viral cRNA"
                     /isolate="Haiti/1/2016"
                     /isolation_source="saliva and urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:64320"
                     /country="USA"
                     /collection_date="05-Feb-2016"
                     /identified_by="John Lednicky and Sarah White"
                     /note="passage details: one passage in LLC-MK2 cells"
     5'UTR           1..107
     CDS             108..10379
                     /codon_start=1
                     /product="polyprotein"
                     /protein_id="AMX81919.1"
                     /db_xref="GI:1016884665"
                     /translation="MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPI
                     RMVLAILAFLRFTAIKPSLGLINRWGSVGKKEAMEIIKKFKKDLAAMLRIINARKEKK
                     RRGADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQI
                     MDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVT
                     LPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKV
                     IYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE
                     LVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG
                     NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET
                     DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF
                     HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE
                     MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG
                     TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG
                     EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG
                     AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC
                     SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR
                     MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGK
                     SHFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE
                     CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI
                     EESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTR
                     GPSLRSTTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKEPESNLVRSMVTA
                     GSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLA
                     ILMGATFAEMNTGGDVAHLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQT
                     AISALEGDLMVLINGFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGL
                     ATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSE
                     VLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKD
                     AEVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGAWY
                     VYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMW
                     HVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARN
                     IQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAIT
                     QGRREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKTRLRTVILAP
                     TRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLY
                     IMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEV
                     PERAWSSGFDWVTDYSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKT
                     KHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQR
                     RGRIGRNPNKPGDEYLYGGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADK
                     VAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIM
                     EDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTL
                     PGHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFV
                     LMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPEPEKQRSP
                     QDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATMGFSMDIDLRPA
                     SAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPL
                     LMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRTAAGIMKNPVVDGIV
                     VTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSP
                     NKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQ
                     MSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKLRWLVERGYLQPYGK
                     VIDLGCGRGGWSYYAATIRKVQEVKGYTKGGPGHEEPVLVQSYGWNIVRLKSGVDVFH
                     MAAEPCDTLLCDIGESSSSPEVEEARTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMM
                     ETLERLQRRYGGGLVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRP
                     VKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG
                     SYEAPTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPD
                     PQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEKEWKTAV
                     EAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGA
                     RFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSRIPGGRMYADDTAGWD
                     TRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQ
                     DQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDR
                     LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHH
                     FNKLHLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRR
                     DLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKT
                     PVTKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST
                     QVRYLGEEGSTPGVL"
     3'UTR           10380..10807
ORIGIN      
        1 agttgttact gttgctgact cagactgcga cagttcgagt ttgaagcgaa agctagcaac
       61 agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa
      121 aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga
      181 gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca
      241 ggatggtctt ggcgattcta gcctttttga gattcacggc aatcaagcca tcactgggtc
      301 tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca
      361 agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag
      421 gcgcagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg
      481 tcactagacg tgggagtgca tactatatgt acttggacag aaacgatgct ggggaggcca
      541 tatcttttcc aaccacattg gggatgaata agtgttatat acagatcatg gatcttggac
      601 acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtggaaccag
      661 atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc
      721 acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta
      781 ggaagctgca aacgcggtcg caaacctggt tggaatcaag agaatacaca aagcacttga
      841 ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg
      901 cttggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga
      961 ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta
     1021 tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg
     1081 cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg
     1141 aggtaagatc ctactgctat gaggcatcaa tatcagacat ggcttcggac agccgctgcc
     1201 caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa
     1261 cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga
     1321 catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc
     1381 tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg
     1441 acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa
     1501 gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag
     1561 gccttgactt ttcagatttg tattacttga ctatgaataa caagcactgg ttggtccaca
     1621 aggagtggtt ccacgacatt ccattacctt ggcacgctgg ggcagacacc ggaactccac
     1681 actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg
     1741 tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg
     1801 ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa
     1861 tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca
     1921 ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga
     1981 cagatggacc ttgcaaggtt ccagctcaga tggcggtgga catgcaaact ctgaccccag
     2041 ttgggaggtt gataaccgct aaccccgtaa tcactgaaag cactgagaac tctaagatga
     2101 tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga
     2161 agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg
     2221 tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg
     2281 gaggcgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat
     2341 cattgtttgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctgatgtggt
     2401 tgggtctgaa cacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt
     2461 tgatcttctt atccacagcc gtctctgctg atgtggggtg ctcggtggac ttctcaaaga
     2521 aggagacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca
     2581 ggtacaagta ccatcctgac tccccccgta gattggcagc agcagtcaag caagcctggg
     2641 aagatggtat ctgcgggatc tcctctgttt caagaatgga aaacatcatg tggagatcag
     2701 tagaagggga gctcaacgca atcttggaag agaatggagt tcaactgacg gtcgttgtgg
     2761 gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc
     2821 tgccccacgg ctggaaggct tgggggaaat cgcacttcgt cagagcagca aagacaaata
     2881 acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga
     2941 acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg
     3001 ttagagaaga ttattcatta gagtgtgatc cagccgttat tggaacagct gttaagggga
     3061 aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaat gacacatgga
     3121 ggctgaagag ggcccatctg atcgagatga aaacatgtga atggccaaag tcccacacat
     3181 tgtggacaga tggaatagaa gagagtgatc tgatcatacc caagtcttta gctgggccac
     3241 tcagccatca caataccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg
     3301 aagagcttga aattcggttt gaggaatgcc cgggcactaa ggtccacgtg gaggaaacat
     3361 gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat
     3421 ggtgctgcag ggagtgcaca atgcccccac tgtcgttccg ggctaaagat ggctgttggt
     3481 atggaatgga gataaggccc aggaaagaac cagaaagcaa cttagtaagg tcaatggtga
     3541 ctgcaggatc aactgatcac atggatcact tctcccttgg agtgcttgtg attctgctca
     3601 tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg
     3661 cagtgctggt agctatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa
     3721 ttttgatggg cgccaccttc gcggaaatga acactggagg agatgtagct catctggcgc
     3781 tgatagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt
     3841 ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct
     3901 ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa
     3961 tacgagcgat ggttgttcca cgcactgata acatcacctt ggcaatcctg gctgctctga
     4021 caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg
     4081 ggtttatgct cctctctctg aagggaaaag gcagtgtgaa gaagaactta ccatttgtca
     4141 tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg gggctgctgt
     4201 tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc
     4261 tgatatgcgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg
     4321 ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca
     4381 ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agtcactgga aacagtcccc
     4441 ggctcgatgt ggcgctagat gagagtggtg atttctccct ggtggaggat gacggtcccc
     4501 ccatgagaga gatcatactc aaggtggtcc tgatgaccat ctgtggcatg aacccaatag
     4561 ccataccctt tgcagctgga gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg
     4621 ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt
     4681 acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag
     4741 agggggtctt tcacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag
     4801 ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat
     4861 ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg
     4921 gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca
     4981 ttggagcggt tgcgctggat tacccagcag gaacttcagg atctccaatc ctagacaagt
     5041 gtgggagagt gataggactt tatggcaatg gggtcgtgat caaaaatggg agttatgtta
     5101 gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga
     5161 tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga
     5221 gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag
     5281 ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt
     5341 atatgacaac agcagtcaat gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc
     5401 atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata
     5461 ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa
     5521 caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc
     5581 gtgacgcatt tccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga
     5641 gagcctggag ctcaggcttt gattgggtga cggattattc tggaaaaaca gtttggtttg
     5701 ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg
     5761 tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt
     5821 gggactttgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg
     5881 tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattttgg
     5941 ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga
     6001 atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgacgaag
     6061 accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc
     6121 tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca
     6181 agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg
     6241 tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct
     6301 ttgatggcac gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca
     6361 gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc
     6421 atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag
     6481 tgatggaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg
     6541 acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg
     6601 cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc
     6661 tgggaatctt tttcgtcttg atgaggaaca agggcatagg gaagatgggc tttggaatgg
     6721 tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg
     6781 catgtgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc
     6841 aaagatctcc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg
     6901 gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc
     6961 taatgggaag gagagaggag ggggcaacca tgggattctc aatggacatt gacctgcggc
     7021 cagcctcagc ttgggccatc tatgctgcct tgacaacttt cattacccca gccgtccaac
     7081 atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag
     7141 tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc
     7201 taatgatagg ttgctactca caattaacgc ccctgaccct aatagtggcc atcattttgc
     7261 tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc
     7321 agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg
     7381 acattgacac aatgacaatt gacccccaag tggagaaaaa gatgggacag gtgctactca
     7441 tagcagtagc agtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg
     7501 gggccctgat cacagccgca acttccactt tgtgggaagg ctctccgaac aagtactgga
     7561 actcctctac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt
     7621 ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag
     7681 gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct
     7741 actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca
     7801 aggacggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt
     7861 tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag
     7921 ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa
     7981 aaggaggccc tggtcatgaa gaacccgtgt tggtgcaaag ctatgggtgg aacatagtcc
     8041 gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt
     8101 gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc
     8161 tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt
     8221 gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag
     8281 gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag
     8341 cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg
     8401 acgggcctag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg
     8461 ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga
     8521 tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg
     8581 cttaccatgg aagctatgag gcccccacac aagggtcagc gtcctctcta ataaacgggg
     8641 ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga
     8701 ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc
     8761 cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag
     8821 agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc
     8881 gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg
     8941 aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga
     9001 gaggagagtg ccagagttgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg
     9061 aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc
     9121 tagagttcga agcccttgga ttcttgaacg aggatcactg gatggggaga gagaactcag
     9181 gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc
     9241 gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca
     9301 ggtttgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg cacagggcct
     9361 tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag
     9421 ctgaaaaagg gaagacagtt atggacatta tttcgagaca agaccaaagg gggagcggac
     9481 aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata
     9541 tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag
     9601 tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag
     9661 atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg
     9721 atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact
     9781 gggaagaagt tccgttttgc tcccaccact tcaacaagct ccatctcaag gacgggaggt
     9841 ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgc gtctctccag
     9901 gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc
     9961 agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg
    10021 tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat
    10081 ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc
    10141 acatggaaga caagacccca gttacgaaat ggacagacat tccctatttg ggaaaaaggg
    10201 aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca
    10261 ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact
    10321 acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctgtaag
    10381 caccaatctt aatgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc
    10441 ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg
    10501 gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca
    10561 tgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg
    10621 ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggagccc
    10681 cccggaaaac gcaaaacagc atattgacgc tgggaaagac cagagactcc atgagtttcc
    10741 accacgctgg ccgccaggca cagatcgccg aatagcggcg gccggtgtgg ggaaatccat
    10801 gggtctt
Posted
Sequences producing significant alignments:

Select:AllNone Selected:0

Sequences producing significant alignments:
Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccession
1848018480100%0.099%KU509998.2
1845618456100%0.099%KU321639.1
1843818438100%0.099%KU527068.1
1842918429100%0.099%KJ776791.1
1841118411100%0.099%KU991811.1
1840218402100%0.099%KU729218.1
1840218402100%0.099%KU707826.1
1840218402100%0.099%KU365779.1
1839018390100%0.099%KU926309.1
1839018390100%0.099%KU365780.1
1838418384100%0.099%KU940228.1
1838418384100%0.099%KU647676.1
1838418384100%0.099%KU365777.1
1838118381100%0.099%KU501217.1
183771837799%0.099%KU497555.1
1837518375100%0.099%KU729217.2
1837518375100%0.099%KU501216.1
1837218372100%0.099%KU820897.1
1837218372100%0.099%KU365778.1
1837218372100%0.099%KU312312.1
1836618366100%0.099%KU922960.1
1836318363100%0.099%KU926310.1
1836318363100%0.099%KU922923.1
1836318363100%0.099%KU501215.1
1835718357100%0.099%KU870645.1
1835418354100%0.099%KU853013.1
1835218352100%0.099%KU853012.1
1834518345100%0.099%KU820898.1
1833918339100%0.099%KU955590.1
1833618336100%0.099%KU740184.2
1833618336100%0.099%KU761564.1
1833018330100%0.099%KU963796.1
1832718327100%0.099%KU955589.1
1832718327100%0.099%KU820899.2
182911829199%0.099%KU940224.1
1821818218100%0.099%KU866423.1
1821318213100%0.099%KU744693.1
1808718087100%0.099%KU681081.3
1778617786100%0.098%KU955593.1
177841778499%0.098%JN860885.1
177331773398%0.099%KF993678.1
1762517625100%0.098%EU545988.1
1747917479100%0.098%KU681082.3
164511645199%0.096%HQ234499.1
1328813288100%0.089%KF383115.1
1327913279100%0.089%KF268949.1
1327913279100%0.089%KF268948.1
1327713277100%0.089%KU720415.1
1327213272100%0.089%KF268950.1
132721327299%0.089%HQ234498.1
1326313263100%0.089%KU955595.1
1326113261100%0.089%DQ859059.1
1325913259100%0.089%KF383119.1
1325413254100%0.089%KU955592.1
1325413254100%0.089%LC002520.1
1323813238100%0.089%KU963573.1
1323813238100%0.089%KU955594.1
1323613236100%0.089%KU955591.1
1321213212100%0.089%KF383116.1
132051320599%0.089%HQ234501.1
1319613196100%0.089%AY632535.2
1313313133100%0.088%KF383117.1
1312613233100%0.088%KU963574.1
131261312699%0.088%HQ234500.1
1293613004100%0.088%KF383118.1
128551285597%0.089%KF383121.1
108441084497%0.084%KF383120.1
50471445687%0.095%KU940227.1
4985498527%0.099%KU312314.1
4958495827%0.099%KU312313.1
4650465025%0.099%KU646828.1
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