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23andMe Granted First FDA Authorization for Direct-to-Consumer Genetic Test on Cancer Risk March 6, 2018 Authorization allows 23andMe to report on BRCA1- and BRCA2-related genetic risk for breast, ovarian and prostate cancer Mountain View, California – March 6, 2018 – 23andMe, Inc., the leading personal genetics company, today received the first-ever FDA authorization for a direct-to-consumer genetic test for cancer risk. The authorization allows 23andMe to provide customers, without a prescription, information on three genetic variants found on the BRCA1 and BRCA2 genes known to be associated with higher risk for breast, ovarian and prostate cancer. “Being the first and only direct-to-consumer genetics company to receive FDA authorization to test for cancer risk without a prescription is a major milestone for 23andMe and for the consumer,” said Anne Wojcicki, 23andMe CEO and co-founder. “We believe it’s important for consumers to have direct and affordable access to this potentially life-saving information. We will continue pioneering a path for greater access to health information, and promoting a more consumer-driven, preventative approach to health care.” 23andMe will report on three variants in the BRCA1 and BRCA2 genes associated with a significantly higher risk of breast and ovarian cancer in women, and breast cancer in men. The variants may also be associated with an increased risk for certain other cancers. These variants are most prevalent in those of Ashkenazi Jewish descent, and have been observed at much lower rates in other ethnicities. About 1 in 40 individuals of Ashkenazi Jewish descent has one of these three variants. Women with one of these variants have a 45-85% chance of developing breast cancer by age 70. “This authorization is incredibly valuable for those who might not be aware of their Ashkenazi Jewish descent or aren’t familiar with their family history of cancer,” said Wojcicki. “But it’s important to understand that the majority of cancer is not hereditary, our test does not account for all genetic variants that can cause a higher risk of cancer, and people should continue with their recommended cancer screenings.” New and existing 23andMe Health + Ancestry Service customers that were genotyped on the company’s most recent platforms will have access to this report in the coming weeks. As with select other Genetic Health Risk reports, customers must specifically choose for themselves if and when they want to receive this information. The report also includes an education module to ensure customers are fully informed on what they can learn from this report and how to use the results. The 23andMe Personal Genome Service® submission for the BRCA1/BRCA2 (Selected Variants) Genetic Health Risk Report was extensively evaluated through the FDA de novo classification pathway, a regulatory process enabling classification and authorization for novel moderate-risk medical devices (FDA authorization # DEN170046). As part of the review process in order to establish safety and effectiveness for this authorization, 23andMe demonstrated a high level of accuracy (greater than 99% concordance to Sanger sequencing) and precision (demonstrated by studies yielding greater than 99% reproducibility and repeatability). The 23andMe BRCA1/BRCA2 (Selected Variants) Genetic Health Risk Report utilizes the same informational concepts previously demonstrated in studies submitted to the FDA for its Genetic Health Risk reports which were shown to have 90% or greater overall user comprehension in a demographically diverse population study. Current clinical guidelines for genetic risk evaluation are based primarily on personal and/or family history of certain cancers. However, a perspective by Mary Claire-King, Ph.D. (who received the Lasker award in 2014 in recognition of her pioneering discovery of the BRCA1 gene) published in The Journal of the American Medical Association has argued for broader access, citing a study where “50 percent of families found to harbor BRCA1 or BRCA2 mutations had no history of breast or ovarian cancer that would have triggered clinical attention.” (https://jamanetwork.com/journals/jama/fullarticle/1902783) Consistent with those previous findings, 23andMe found that of those BRCA carriers who gave family medical history, about half reported no history of cancer in first-degree relatives. 23andMe has also observed that many of its customers for whom Ashkenazi Jewish ancestry was detected, did not self report any Jewish ancestry. These individuals might fall outside the guidelines of clinical testing. With this authorization, 23andMe continues to pioneer consumer access to genetic information, and remains the only company able to offer genetic health risk reports without a prescription. This authorization is the third de novo authorization granted by the FDA to 23andMe. In February 2015, 23andMe was granted authorization by the FDA to market the first direct-to-consumer genetic test for Bloom Syndrome under the de novo pathway which has since enabled the company to bring 40+ carrier status reports directly to customers. These reports convey risk for diseases that may be passed to offspring. In April 2017, 23andMe was granted authorization by the FDA to market the first direct-to-consumer genetic health risk reports, to date the Company has launched 9 reports for a variety of genetic health risks. The intended use of 23andMe BRCA1/BRCA2 (Selected Variants) Genetic Health Risk Report: Indications for Use: The 23andMe Personal Genome Service (PGS) uses qualitative genotyping to detect select clinically relevant variants in genomic DNA isolated from human saliva collected from individuals ≥ 18 years with the Oragene Dx model OGD500.001 for the purpose of reporting and interpreting genetic health risks, including the 23andMe PGS Genetic Health Risk Report for BRCA1/BRCA2 (Selected Variants). The 23andMe PGS Genetic Health Risk Report for BRCA1/BRCA2 (Selected Variants) is indicated for reporting of the 185delAG and 5382insC variants in the BRCA1 gene and the 6174delT variant in the BRCA2 gene. The report describes if a woman is at increased risk of developing breast and ovarian cancer, and if a man is at increased risk of developing breast cancer or may be at increased risk of developing prostate cancer. The three variants included in this report are most common in people of Ashkenazi Jewish descent and do not represent the majority of the BRCA1/BRCA2 variants in the general population. The test report does not describe a person’s overall risk of developing any type of cancer, and the absence of a variant tested does not rule out the presence of other variants that may be cancer-related. This test is not a substitute for visits to a healthcare provider for recommended screenings or appropriate follow-up and should not be used to determine any treatments. About 23andMe 23andMe, Inc. is the leading consumer genetics and research company. Founded in 2006, the mission of the company is to help people access, understand and benefit from the human genome. The company was named by MIT Technology Review in its “50 Smartest Companies, 2017” list, and featured as Fast Company's #2 Most Innovative Health Company in 2018. 23andMe has millions of customers worldwide, with more than 80 percent of customers consented to participate in research. 23andMe, Inc. is located in Mountain View, CA. More information is available at www.23andMe.com. https://mediacenter.23andme.com/press-releases/23andme-granted-first-fda-authorization-direct-consumer-genetic-test-cancer-risk/

FDA Authorizes 23andMe DTC Test Report for Three BRCA Mutations Mar 06, 2018 | staff reporter NEW YORK (GenomeWeb) – The US Food and Drug Administration today authorized 23andMe's genetic health risk report for three BRCA1 and BRCA2 mutations most commonly found in people of Ashkenazi Jewish descent. "This test provides information to certain individuals who may be at increased breast, ovarian, or prostate cancer risk and who might not otherwise get genetic screening, and is a step forward in the availability of DTC genetic tests. But it has a lot of caveats," Donald St. Pierre, acting director of the Office of In Vitro Diagnostics and Radiological Health within FDA's Center for Devices and Radiological Health, said in a statement. "While the detection of a BRCA mutation on this test does indicate an increased risk, only a small percentage of Americans carry one of these three mutations and most BRCA mutations that increase an individual's risk are not detected by this test. The test should not be used as a substitute for seeing your doctor for cancer screenings or counseling on genetic and lifestyle factors that can increase or decrease cancer risk." Approximately 2 percent of women of Ashkenazi Jewish women have the three BRCA1/BRCA2 hereditary mutations, but they occur very rarely in other ethnic populations, in about 0.1 percent of individuals, if at all. All individuals, regardless of their ethnicity, may have other BRCA1/2 mutations or cancer-linked mutations in other genes not gauged by 23andMe's test. There are well over 1,000 BRCA mutations associated with breast and ovarian cancer risk, and dozens of other genes associated with cancer more broadly. Most cancer cases, the FDA added, are due to non-genetic risk factors, such as obesity, smoking, and hormone use, and patients should consult their healthcare provider to discuss efforts to modify these risks. "The test … does not provide information on a person's overall risk of developing any type of cancer," the FDA stated. "The use of the test carries significant risks if individuals use the test results without consulting a physician or genetic counselor." The FDA further noted that consumers and health care professionals should not use 23andMe's test results to make treatment decisions, including anti-hormone therapies and prophylactic removal of the breasts or ovaries, and that such decisions require confirmatory testing and genetic counseling. In order to authorize 23andMe's BRCA test reports, the FDA reviewed data the company submitted via the de novo premarket review pathway for low-to-moderate-risk devices that are not substantially equivalent to a marketed device. The agency said it is also publishing special controls to outline its expectations for the test's accuracy, reproducibility, clinical performance, and labeling. Previously the FDA has authorized 23andMe's Bloom syndrome test report, which opened the door for the company to broadly market carrier screening testing directly to consumers. Last year, the agency authorized the first crop of 23andMe's genetic health risk reports for DTC sales, but specifically restricted the intended use of such tests in prenatal testing, in determining cancer risk that leads to prophylactic screening or treatment, determining autosomal dominant variants, and pharmacogenetic testing. https://www.genomeweb.com/cancer/fda-authorizes-23andme-dtc-test-report-three-brca-mutations?utm_source=Sailthru&utm_medium=email&utm_campaign=GWDN Tues AM 2018-03-06&utm_term=GW Daily News Bulletin#.WqsQqqjwbFh

23andme has received FDA approval on the three most well studied frame shifts in BRCA1 and BRCA2. These frame shifts are founder mutations in Ashkenazi Jews and are present in approximately 2% of the population.

Isolate name: A/chicken/Kostroma/1719/2017 Isolate ID: EPI_ISL_297464 Passage details/history: original Type: A / H5N2 Lineage: Sample information Collection date: 2017-12-22 Host Gallus gallus domesticus Additional host information: Domestic status: Domestic Is vaccinated: Location: Russian Federation / Kostroma Oblast Additional location information: Health status: Dead Specimen source: Organ Strain or commercial product name used for vaccination: Institute information Originating lab: Sample ID given by the sample provider: Address: Submitting lab: State Research Center of Virology and Biotechnology (VECTOR) Sample ID given by the submitting laboratory: Authors: Ivan,Susloparov; Natalya,Goncharova; Natalya,Kolosova; Vasiliy,Marchenko; Alexander,Ryzhikov Address: State Research Center of Virology and Biotechnology (VECTOR) Emerging Zoonotic Diseases and Influenza Vector Street Build 12 630559 Koltsovo Russian Federation Publication Publication In vivo antiviral resistance Phenotype Genotype Unspecified Antiviral resistance tested by experimental procedures Adamantanes: Unknown Oseltamivir: Unknown Zanamivir: Unknown Peramivir: Unknown Other: Unknown Additional information Antigenic characterization: Note: Sequence segment identifier length accession # INSDC Sequence PB2 A/chicken/Kostroma/1719/2017 2329 EPI1170197 PB1 A/chicken/Kostroma/1719/2017 2329 EPI1170196 PA A/chicken/Kostroma/1719/2017 2221 EPI1170190 HA A/chicken/Kostroma/1719/2017 1776 EPI1170191 NP A/chicken/Kostroma/1719/2017 1565 EPI1170194 NA A/chicken/Kostroma/1719/2017 1394 EPI1170193 MP A/chicken/Kostroma/1719/2017 1027 EPI1170192 NS A/chicken/Kostroma/1719/2017 890 EPI1170195 Submitter information Submitter: Susloparov, Ivan Submission Date: 2018-02-09 Last modifier: Susloparov, Ivan Last modified: 2018-02-11 Address: State Research Center of Virology and Biotechnology (VECTOR) Emerging Zoonotic Diseases and Influenza Vector Street Build 12 630559 Koltsovo Russian Federation

Isolate name: A/chicken/Kostroma/1720/2017 Isolate ID: EPI_ISL_297465 Passage details/history: original Type: A / H5N2 Lineage: Sample information Collection date: 2017-12-22 Host Gallus gallus domesticus Additional host information: Domestic status: Domestic Is vaccinated: Location: Russian Federation / Kostroma Oblast Additional location information: Health status: Dead Specimen source: Organ Strain or commercial product name used for vaccination: Institute information Originating lab: Sample ID given by the sample provider: Address: Submitting lab: State Research Center of Virology and Biotechnology (VECTOR) Sample ID given by the submitting laboratory: Authors: Ivan,Susloparov; Natalya,Goncharova; Natalya,Kolosova; Vasiliy,Marchenko; Alexander,Ryzhikov Address: State Research Center of Virology and Biotechnology (VECTOR) Emerging Zoonotic Diseases and Influenza Vector Street Build 12 630559 Koltsovo Russian Federation Publication Publication In vivo antiviral resistance Phenotype Genotype Unspecified Antiviral resistance tested by experimental procedures Adamantanes: Unknown Oseltamivir: Unknown Zanamivir: Unknown Peramivir: Unknown Other: Unknown Additional information Antigenic characterization: Note: Sequence segment identifier length accession # INSDC Sequence PB2 A/chicken/Kostroma/1720/2017 2329 EPI1170206 PB1 A/chicken/Kostroma/1720/2017 2329 EPI1170205 PA A/chicken/Kostroma/1720/2017 2221 EPI1170198 HA A/chicken/Kostroma/1720/2017 1776 EPI1170199 NP A/chicken/Kostroma/1720/2017 1565 EPI1170203 NA A/chicken/Kostroma/1720/2017 1394 EPI1170201 MP A/chicken/Kostroma/1720/2017 1027 EPI1170200 NS A/chicken/Kostroma/1720/2017 890 EPI1170204 Submitter information Submitter: Susloparov, Ivan Submission Date: 2018-02-09 Last modifier: Susloparov, Ivan Last modified: 2018-02-11 Address: State Research Center of Virology and Biotechnology (VECTOR) Emerging Zoonotic Diseases and Influenza Vector Street Build 12 630559 Koltsovo Russian Federation

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176518 A/chicken/Israel/881/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1159825 A/Cygnus atratus/Hubei/HF-1/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1159809 A/Anser cygnoides/Hubei/FW44/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI1159817 A/Cygnus atratus/Hubei/2Z2-O/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3077.6 0.000000e+00 1690/1702 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1021106 A/mute swan/Czech Republic/1296-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1006681 A/common teal/Korea/W549/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1006680 A/common teal/Korea/W547/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI931207 A/turkey/Germany-BB/R234ff/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI869936 A/chicken/Kalmykia/2661/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI858836 A/duck/India/10CA01/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI836614 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI836606 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774394 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774386 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774310 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774242 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774234 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774121 A/Bar-headed Goose/Qinghai/BTY1-LV/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774113 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1176519 A/chicken/Israel/1048/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1169147 A/chicken/Rostov-on-Don/1321/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019678 A/Grey_Go/NL-Groot-Ammers/16015901-012/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019646 A/Eur_Wig/NL-Zwolle/16015820-002/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019630 A/Eur_Wig/NL-Wormer/16016143-002/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019622 A/Eur_Wig/NL-West Graftdijk/16015746-003/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019614 A/Eur_Wig/NL-Walterswald/16015923-003/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019606 A/Eur_Wig/NL-Vianen/16015917-006/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019590 A/Eur_Wig/NL-Reeuwijk/16015903-003/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019430 A/Ch/NL-Rhenen/16016141-006/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019406 A/Ch/NL-Boven Leeuwen/16016151-006-010/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019398 A/Ch/NL-Abbega/X16015736/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1007675 A/peacock/Belgium/1017/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1006705 A/common teal/Korea/W555/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1006704 A/common teal/Korea/W550/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1006703 A/common teal/Korea/W548/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI959413 A/Eurasian Wigeon/Netherlands/4/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954607 A/turkey/Italy/17VIR1452-22/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954575 A/turkey/Italy/17VIR576-11/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI942943 A/chicken/Wales/000023/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774475 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774458 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774450 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774402 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774378 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774326 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774318 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774302 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774277 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774267 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774259 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774251 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774226 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774218 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774210 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774201 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774193 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774185 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774176 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774168 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774159 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774150 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI978865 A/mute swan/Germany-TH/R1126/2017 (A/H5N8) segment 4 (HA) 3068.4 0.000000e+00 1689/1704 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 3066.6 0.000000e+00 1689/1704 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3066.6 0.000000e+00 1689/1704 (99%) EPI1176527 A/turkey/Israel/1076/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1045611 A/chicken/Rostov-on-Don/44/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032524 A/Mulard_duck/Hungary/60369/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032508 A/Goose/Hungary/17985/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032492 A/Goose/Hungary/17051/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%)

LOCUS MF166575 1717 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/chicken/Israel/881/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166575 VERSION MF166575.1 KEYWORDS . SOURCE Influenza A virus (A/chicken/Israel/881/2016(H5N8)) ORGANISM Influenza A virus (A/chicken/Israel/881/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1717) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1717) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1717 /organism="Influenza A virus (A/chicken/Israel/881/2016(H5N8))" /mol_type="viral cRNA" /strain="A/chicken/Israel/881/2016" /serotype="H5N8" /host="chicken" /db_xref="taxon:2005432" /segment="4" /country="Israel" /collection_date="07-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45891.1" /translation="MEKIVLLLAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNDAYPTIKISYNNTNREDLLILWGIHHSNNAEEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKG DSTIMKSEVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVLATGLR NSPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSDGSLQCRVC V" sig_peptide 1..48 /gene="HA" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttagccttg ttaaaagtga tcagatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgatcta 181 aatggggtga aacctctgat tttaaaggat tgtagtgtag ctggatggct cctcggaaac 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaac 301 ccagctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagctc ttggcccaat 421 catgaaacat cattaggggt gagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggcttat caaaaagaac gatgcatatc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggga ttcatcattc caacaatgca 601 gaagagcaga caaatctcta taaaaaccca accacctata tttcagttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgtgga 721 agaatggact tcttctggac aattttaaaa ccgaatgatg caatccattt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt 841 atgaaaagtg aagtggaata tggccactgc aacaccaaat gtcaaacccc agtaggagcg 901 ataaactcta gtatgccatt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtccttgcg actgggctca gaaatagtcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg gggatggcag 1081 ggaatggttg atggttggta tgggtaccac catagcaatg agcaggggag tgggtacgct 1141 gcagacaaag aatccaccca aaaggcaata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt acgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg agttctatca caaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatagg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc cgatgggtcg 1681 ttacagtgca gagtttgcgt ttaaatttgt gagatca

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176529 A/grey goose/Israel/986/2016 (A/H5N8) segment 4 (HA) 3009.3 0.000000e+00 1629/1629 (100%) EPI1176519 A/chicken/Israel/1048/2016 (A/H5N8) segment 4 (HA) 2992.7 0.000000e+00 1626/1629 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 2970.5 0.000000e+00 1622/1629 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 2970.5 0.000000e+00 1622/1629 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 2970.5 0.000000e+00 1622/1629 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 2970.5 0.000000e+00 1622/1629 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1621/1629 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1621/1629 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1621/1629 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1621/1629 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1621/1629 (99%) EPI1021106 A/mute swan/Czech Republic/1296-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1620/1629 (99%) EPI978865 A/mute swan/Germany-TH/R1126/2017 (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1620/1629 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1620/1629 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1620/1629 (99%) EPI931207 A/turkey/Germany-BB/R234ff/2017 (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1620/1629 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1620/1629 (99%) EPI869936 A/chicken/Kalmykia/2661/2016 (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1620/1629 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 2959.5 0.000000e+00 1621/1630 (99%) EPI1169147 A/chicken/Rostov-on-Don/1321/2017 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI1021132 A/goose/Czech Republic/1954-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI964917 A/chicken/Czech Republic/206-17_2/2017 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI887468 A/chicken/Czech Republic/206-17_2/2017(H5N8) (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI859207 A/domestic_turkey/Hungary/53433/2016 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1619/1629 (99%) EPI1176527 A/turkey/Israel/1076/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1032524 A/Mulard_duck/Hungary/60369/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1032508 A/Goose/Hungary/17985/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1032492 A/Goose/Hungary/17051/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1032484 A/Goose/Hungary/15729/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1032476 A/Goose/Hungary/17261/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1021135 A/quail/Czech Republic/2063-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI978863 A/grey heron/Germany-TH/R1125/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI962066 A/Turkey/Hungary/53136/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI962046 A/Duck/Hungary/54738/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI959539 A/Mute swan/Hungary/6276/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI959531 A/Mute swan/Hungary/6092/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI954655 A/Duck/Hungary/984/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI954639 A/Harris Hawk/Hungary/120/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI952782 A/mallard duck/Korea/WA137/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI952639 A/chicken/Korea/H903/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI930838 A/mute swan/Czech Republic/54-17_2/2017 (H5N8) (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI919636 A/chicken/Czech Republic/585-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI891670 A/greylag goose/Croatia/33/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI881901 A/mute swan/Croatia/15/2017 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI869929 A/mute swan/Poland/108/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI866978 A/duck/Hungary/60441/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI861572 A/mute swan/Croatia/78/2016 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1618/1629 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 2946.5 0.000000e+00 1614/1624 (99%) EPI1021086 A/mute swan/Czech Republic/54-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2944.7 0.000000e+00 1617/1629 (99%) EPI869926 A/domestic goose/Poland/72/2016 (A/H5N8) segment 4 (HA) 2944.7 0.000000e+00 1617/1629 (99%) EPI1176530 A/peregrine falcon/Israel/1086/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1169885 A/chicken/Republic of Macedonia/466/2017 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1159809 A/Anser cygnoides/Hubei/FW44/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1605/1611 (99%) EPI1081894 A/turkey/Czech Republic/38-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1032548 A/Goose/Hungary/64909/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1032540 A/Goose/Hungary/63743/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1032516 A/Goose/Hungary/59763/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1032500 A/Goose/Hungary/17580/2017 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021139 A/mallard/Czech Republic/2641-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021126 A/mute swan/Czech Republic/1691-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021125 A/mallard/Czech Republic/1690-17_2/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021103 A/mallard/Czech Republic/1219-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021098 A/mute swan/Czech Republic/1155-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021097 A/mute swan/Czech Republic/1060-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021088 A/mallard/Czech Republic/136-17_2/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1021087 A/goose/Czech Republic/136-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019678 A/Grey_Go/NL-Groot-Ammers/16015901-012/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019646 A/Eur_Wig/NL-Zwolle/16015820-002/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019630 A/Eur_Wig/NL-Wormer/16016143-002/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019622 A/Eur_Wig/NL-West Graftdijk/16015746-003/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019614 A/Eur_Wig/NL-Walterswald/16015923-003/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019606 A/Eur_Wig/NL-Vianen/16015917-006/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019590 A/Eur_Wig/NL-Reeuwijk/16015903-003/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019534 A/Eur_Wig/NL-Enumatil-Groningen/16015704-001/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019430 A/Ch/NL-Rhenen/16016141-006/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019406 A/Ch/NL-Boven Leeuwen/16016151-006-010/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1019398 A/Ch/NL-Abbega/X16015736/2016 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI1007675 A/peacock/Belgium/1017/2017 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI987882 A/chicken/Republic of Macedonia/AR1167-L02131/2017 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI969265 A/chicken/Czech Republic/2644-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI961484 A/mute swan/Czech Republic/581-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI961475 A/mute swan/Czech Republic/499-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%) EPI959555 A/Pheasant/Hungary/7685/2017 (A/H5N8) segment 4 (HA) 2942.8 0.000000e+00 1617/1629 (99%)

LOCUS MF166581 1629 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/grey goose/Israel/986/2016(H5N8)) segment 4 hemagglutinin (HA) gene, partial cds. ACCESSION MF166581 VERSION MF166581.1 KEYWORDS . SOURCE Influenza A virus (A/grey goose/Israel/986/2016(H5N8)) ORGANISM Influenza A virus (A/grey goose/Israel/986/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1629) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1629) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (29-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1629 /organism="Influenza A virus (A/grey goose/Israel/986/2016(H5N8))" /mol_type="viral cRNA" /strain="A/grey goose/Israel/986/2016" /serotype="H5N8" /host="grey goose" /db_xref="taxon:2005436" /segment="4" /country="Israel" /collection_date="12-Dec-2016" /note="passage details: E1" gene <1..1611 /gene="HA" CDS <1..1611 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45897.1" /translation="VDTIMEKNVTVTHAQDILEKTHNGKLCDLNGVKPLILKDCSVAG WLLGNPMCDEFIRVPEWSYIVERANPANDLCYPGSLNDYEELKHLLSRINHFEKILII PKSSWPNHETSLGVSAACPYQGTPSFFRNVVWLIKKNDAYPTIKISYNNTNREDLLIL WGIHHSNNAEEQTNLYKNPTTYISVGTSTLNQRLVPKIATRSQVNGQRGRMDFFWTIL KPNDAIHFESNGNFIAPEYAYKIVKKGDSTIMKSEVEYGHCNTKCQTPVGAINSSMPF HNIHPLTIGECPKYVKSNKLVLATGLRNSPLREKRRKRGLFGAIAGFIEGGWQGMVDG WYGYHHSNEQGSGYAADKESTQKAIDGVTNKVNSIIDKMNTQFEAVGREFNNLERRIE NLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRLQLRDNAKELGNG CFEFYHKCDNECMESVRNGTYDYPQYSEEARLKREEISGVKLESIGTYQILSIYSTVA SSLALAIMVAGLSLWMCSNGSLQCRICI" mat_peptide <1..942 /gene="HA" /product="HA1" mat_peptide 943..1608 /gene="HA" /product="HA2" ORIGIN 1 gttgacacga taatggaaaa gaacgtcact gttacacatg cccaagacat actggaaaaa 61 acacacaacg ggaagctctg cgatctaaat ggggtgaaac ctctgatttt aaaggattgt 121 agtgtagctg gatggctcct cggaaaccca atgtgcgacg aattcatcag agtgccggaa 181 tggtcttaca tagtggagag ggctaacccc gctaatgacc tctgttaccc agggagcctc 241 aatgactatg aagaactgaa acacctgttg agcagaataa atcattttga gaagattctg 301 atcatcccca agagttcttg gcccaatcat gaaacatcat taggggtgag cgcagcttgt 361 ccataccagg gaacgccctc ctttttcaga aatgtggtat ggcttatcaa aaagaacgat 421 gcatacccaa caataaagat aagctacaat aataccaatc gggaagatct cttgatactg 481 tgggggattc atcattccaa caatgcagaa gagcagacaa atctctataa aaacccaacc 541 acctatattt cggttggaac atcaacatta aaccagagat tggtaccaaa aatagctact 601 agatcccaag taaacgggca acgtggaaga atggacttct tctggacaat tttaaaaccg 661 aatgatgcaa tccacttcga gagtaatgga aatttcattg ctccagaata tgcatacaaa 721 attgtcaaga aaggggactc aacaattatg aaaagtgaag tggaatatgg ccactgcaac 781 accaaatgtc aaaccccagt aggagcgata aactctagta tgccattcca caatatacat 841 cctctcacca tcggggaatg ccccaaatac gtgaagtcaa acaagttggt ccttgcgact 901 gggctcagaa atagtcctct aagagaaaag agaagaaaaa gagggctgtt tggggctata 961 gcaggtttta tagagggagg atggcaggga atggttgatg gttggtatgg gtaccaccat 1021 agcaatgagc aggggagtgg gtacgctgca gacaaagaat ccacccaaaa agcaatagat 1081 ggagttacca ataaggtcaa ctcgatcatt gacaaaatga acactcaatt tgaggcagtt 1141 ggaagggagt ttaataactt agaaaggagg atagagaatt tgaacaagaa aatggaagac 1201 ggattcctag atgtctggac ctataatgct gaacttctag ttctcatgga aaacgagagg 1261 actctagatt tccatgactc aaatgtcaag aacctttacg acaaagtcag actgcagctt 1321 agggataatg caaaggagct gggtaacggt tgtttcgagt tctatcacaa atgtgataat 1381 gaatgtatgg aaagtgtgag aaatgggacg tatgactacc ctcagtattc agaagaagca 1441 agattaaaaa gagaagaaat aagcggagtg aaattagaat caataggaac ttaccaaata 1501 ctgtcaattt attcaacagt ggcgagttcc ctagcactgg caatcatggt ggctggtcta 1561 tctttatgga tgtgctccaa tgggtcgtta cagtgcagaa tttgcattta aatttgggag 1621 ctcagattg

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176524 A/cormorant/Israel/1035/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1176517 A/turkey/Israel/1045/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1700/1705 (99%) EPI1176528 A/great egret/Israel/1084/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1699/1705 (99%) EPI1176525 A/great egret/Israel/1088/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1698/1706 (99%) EPI1019630 A/Eur_Wig/NL-Wormer/16016143-002/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1697/1705 (99%) EPI1019614 A/Eur_Wig/NL-Walterswald/16015923-003/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1697/1705 (99%) EPI1007675 A/peacock/Belgium/1017/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1697/1705 (99%) EPI954607 A/turkey/Italy/17VIR1452-22/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1697/1705 (99%) EPI954575 A/turkey/Italy/17VIR576-11/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1697/1705 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3099.8 0.000000e+00 1696/1705 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1045611 A/chicken/Rostov-on-Don/44/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019534 A/Eur_Wig/NL-Enumatil-Groningen/16015704-001/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019526 A/Eur_Wig/NL-Drieborg (Dollard)/16015513-001/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI909444 A/mute swan/Krasnodar/25/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1019718 A/M_Swan/NL-Roggebotsluis/16014462-019/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019678 A/Grey_Go/NL-Groot-Ammers/16015901-012/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019646 A/Eur_Wig/NL-Zwolle/16015820-002/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019622 A/Eur_Wig/NL-West Graftdijk/16015746-003/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019606 A/Eur_Wig/NL-Vianen/16015917-006/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019590 A/Eur_Wig/NL-Reeuwijk/16015903-003/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019430 A/Ch/NL-Rhenen/16016141-006/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019406 A/Ch/NL-Boven Leeuwen/16016151-006-010/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019398 A/Ch/NL-Abbega/X16015736/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1007667 A/chicken/Belgium/807/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI959413 A/Eurasian Wigeon/Netherlands/4/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI942943 A/chicken/Wales/000023/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI922508 A/chicken/Germany-NI/R11406/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI909436 A/goose/Krasnodar/3144/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019766 A/T_Dk/NL-Almeerder Zand/16014341-003/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019478 A/Dk/NL-Kamperveen/16016104-001-005/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019438 A/Ch/NL-Zoeterwoude/16016484-021-025/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI969254 A/Tufted Duck/Switzerland/V237/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI954583 A/chicken/Italy/17VIR653-12/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI909428 A/turkey/Rostov-on-Don/11/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI909404 A/chicken/Voronezh/18/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI909396 A/long-eared owl/Voronezh/16/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI909388 A/Ural owl/Voronezh/14/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI909380 A/long-eared owl/Voronezh/15/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI888088 A/wigeon/Italy/17VIR57-3/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI861011 A/turkey/Germany-SH/R8595/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI860509 A/tufted duck/Germany-SH/R8444/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI860401 A/tufted_duck/Germany/AR8459-L01988/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI859653 A/tufted_duck/Germany/AR8444-L01986/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1023562 A/Eurasian_Wigeon/Netherlands/11/2016 (A/H5N8) segment 4 (HA) 3085.0 0.000000e+00 1691/1701 (99%) EPI1040223 A/chicken/Italy/17VIR3078/2017 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1693/1705 (99%) EPI909364 A/tufted duck/Denmark/17740-1/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1693/1705 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1693/1705 (99%) EPI1019878 A/Teal/NL-Ferwert/16015273-013/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019870 A/T_Dk/NL-Zuidoost Beemster/16014148-009/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019862 A/T_Dk/NL-Zuidoost Beemster/16014148-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019790 A/T_Dk/NL-Rotterdam/16014155-001/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019782 A/T_Dk/NL-Roggebotsluis/16014462-015/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019774 A/T_Dk/NL-Monnickendam/16013865-006-008/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019758 A/Sea_eagle/NL-Assen/16015398-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019750 A/P_falcon/NL-Vrouwenpolder (Zeeland)/16015510-001/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019734 A/Mal/NL-IJsselmuiden/16015448-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019726 A/Magpie/NL-Volendam/16014331-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019710 A/L-bl-ba-gull/NL-Sovon/16014324-014/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019702 A/Gull10/NL-Marker Wadden/16014466-014/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019670 A/Gr_bk_bd_gull/NL-Slootdorp/16014102-005/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019542 A/Eur_Wig/NL-Ferwert/16015273-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019446 A/Crow/NL-Oostwoud/16015372-004/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019382 A/Buzzard/NL-Durgerdam/16015100-004/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI990770 A/eurasian wigeon/Germany-NI/AR249-L02143/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI954783 A/Common_tern/Hungary/8187/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI909420 A/chicken/Voronezh/20/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI909412 A/chicken/Voronezh/19/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI863865 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI861224 A/chicken/Germany-SH/R8758/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI860393 A/tufted_duck/Germany/AR8444-L01987/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3077.6 0.000000e+00 1691/1703 (99%) EPI1040225 A/turkey/Italy/17VIR5878-3/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%)

LOCUS MF166572 1748 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/cormorant/Israel/1035/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166572 VERSION MF166572.1 KEYWORDS . SOURCE Influenza A virus (A/cormorant/Israel/1035/2016(H5N8)) ORGANISM Influenza A virus (A/cormorant/Israel/1035/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1748 /organism="Influenza A virus (A/cormorant/Israel/1035/2016(H5N8))" /mol_type="viral cRNA" /strain="A/cormorant/Israel/1035/2016" /serotype="H5N8" /host="cormorant" /db_xref="taxon:2005433" /segment="4" /country="Israel" /collection_date="19-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45888.1" /translation="MEKIVLLLAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNDAYPTIKISYNNTNREDLLILWGIHHSNNAKEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKR DSTIMKSGVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVLATGLR NSPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRIC I" sig_peptide 1..48 /gene="HA" mat_peptide 49..1035 /gene="HA" /product="HA1" mat_peptide 1036..1701 /gene="HA" /product="HA2" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttagccttg ttaaaagtga tcagatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgatcta 181 aatggggtga agcctttgat tttaaaggat tgtagtgtag ctggatggct cctcggaaac 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaat 301 ccagctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagttc ttggcccaat 421 catgaaacat cattaggggt aagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggctcat caaaaagaac gatgcatacc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggaa ttcatcattc caacaatgca 601 aaagagcaga caaatctcta taaaaaccca accacctata tttcagttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgtgga 721 agaatggact tcttctggac aattttaaaa ccgaatgatg caatccattt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaagaggga ctcaacaatt 841 atgaaaagtg gagtggaata tggccactgc aacaccaaat gtcaaacccc agtaggagcg 901 ataaactcta gtatgccgtt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtccttgcg actgggctca gaaatagtcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg aggatggcag 1081 ggaatggttg atggttggta tgggtaccac catagcaatg agcaggggag tgggtacgct 1141 gcagacaaag aatccaccca aaaggcaata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt acgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg aattctatca caaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatagg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc caatgggtcg 1681 ttacagtgca gaatttgcat ttaaatttgt gagctcagat tgtagttaaa aacacccttg 1741 tttctact

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176517 A/turkey/Israel/1045/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1176528 A/great egret/Israel/1084/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI1176525 A/great egret/Israel/1088/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI1176524 A/cormorant/Israel/1035/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1700/1705 (99%) EPI1019630 A/Eur_Wig/NL-Wormer/16016143-002/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019614 A/Eur_Wig/NL-Walterswald/16015923-003/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1007675 A/peacock/Belgium/1017/2017 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI954607 A/turkey/Italy/17VIR1452-22/2017 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI954575 A/turkey/Italy/17VIR576-11/2017 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3105.3 0.000000e+00 1696/1704 (99%) EPI1045611 A/chicken/Rostov-on-Don/44/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019534 A/Eur_Wig/NL-Enumatil-Groningen/16015704-001/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019526 A/Eur_Wig/NL-Drieborg (Dollard)/16015513-001/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI909444 A/mute swan/Krasnodar/25/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019718 A/M_Swan/NL-Roggebotsluis/16014462-019/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019678 A/Grey_Go/NL-Groot-Ammers/16015901-012/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019646 A/Eur_Wig/NL-Zwolle/16015820-002/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019622 A/Eur_Wig/NL-West Graftdijk/16015746-003/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019606 A/Eur_Wig/NL-Vianen/16015917-006/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019590 A/Eur_Wig/NL-Reeuwijk/16015903-003/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019430 A/Ch/NL-Rhenen/16016141-006/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019406 A/Ch/NL-Boven Leeuwen/16016151-006-010/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019398 A/Ch/NL-Abbega/X16015736/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1007667 A/chicken/Belgium/807/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI959413 A/Eurasian Wigeon/Netherlands/4/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI942943 A/chicken/Wales/000023/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI922508 A/chicken/Germany-NI/R11406/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI909436 A/goose/Krasnodar/3144/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019766 A/T_Dk/NL-Almeerder Zand/16014341-003/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019478 A/Dk/NL-Kamperveen/16016104-001-005/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019438 A/Ch/NL-Zoeterwoude/16016484-021-025/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI969254 A/Tufted Duck/Switzerland/V237/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI954583 A/chicken/Italy/17VIR653-12/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909428 A/turkey/Rostov-on-Don/11/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909404 A/chicken/Voronezh/18/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909396 A/long-eared owl/Voronezh/16/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909388 A/Ural owl/Voronezh/14/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909380 A/long-eared owl/Voronezh/15/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI888088 A/wigeon/Italy/17VIR57-3/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI861011 A/turkey/Germany-SH/R8595/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI860509 A/tufted duck/Germany-SH/R8444/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI860401 A/tufted_duck/Germany/AR8459-L01988/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI859653 A/tufted_duck/Germany/AR8444-L01986/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1023562 A/Eurasian_Wigeon/Netherlands/11/2016 (A/H5N8) segment 4 (HA) 3090.6 0.000000e+00 1691/1700 (99%) EPI1040223 A/chicken/Italy/17VIR3078/2017 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI909364 A/tufted duck/Denmark/17740-1/2016 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI1019878 A/Teal/NL-Ferwert/16015273-013/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019870 A/T_Dk/NL-Zuidoost Beemster/16014148-009/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019862 A/T_Dk/NL-Zuidoost Beemster/16014148-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019790 A/T_Dk/NL-Rotterdam/16014155-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019782 A/T_Dk/NL-Roggebotsluis/16014462-015/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019774 A/T_Dk/NL-Monnickendam/16013865-006-008/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019758 A/Sea_eagle/NL-Assen/16015398-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019750 A/P_falcon/NL-Vrouwenpolder (Zeeland)/16015510-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019734 A/Mal/NL-IJsselmuiden/16015448-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019726 A/Magpie/NL-Volendam/16014331-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019710 A/L-bl-ba-gull/NL-Sovon/16014324-014/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019702 A/Gull10/NL-Marker Wadden/16014466-014/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019670 A/Gr_bk_bd_gull/NL-Slootdorp/16014102-005/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019542 A/Eur_Wig/NL-Ferwert/16015273-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019446 A/Crow/NL-Oostwoud/16015372-004/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019382 A/Buzzard/NL-Durgerdam/16015100-004/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI990770 A/eurasian wigeon/Germany-NI/AR249-L02143/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI954783 A/Common_tern/Hungary/8187/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909420 A/chicken/Voronezh/20/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909412 A/chicken/Voronezh/19/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI863865 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI861224 A/chicken/Germany-SH/R8758/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI860393 A/tufted_duck/Germany/AR8444-L01987/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1691/1702 (99%) EPI1040225 A/turkey/Italy/17VIR5878-3/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%)

LOCUS MF166574 1748 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/turkey/Israel/1045/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166574 VERSION MF166574.1 KEYWORDS . SOURCE Influenza A virus (A/turkey/Israel/1045/2016(H5N8)) ORGANISM Influenza A virus (A/turkey/Israel/1045/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1748 /organism="Influenza A virus (A/turkey/Israel/1045/2016(H5N8))" /mol_type="viral cRNA" /strain="A/turkey/Israel/1045/2016" /serotype="H5N8" /host="turkey" /db_xref="taxon:2005438" /segment="4" /country="Israel" /collection_date="20-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45890.1" /translation="MEKIVLLLAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNNAYPTIKISYNNTNREDLLILWGIHHSNNAEEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKG DSTIMKSGVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVFATGLR NSPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRIC I" sig_peptide 1..48 /gene="HA" mat_peptide 49..1035 /gene="HA" /product="HA1" mat_peptide 1036..1701 /gene="HA" /product="HA2" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttagccttg ttaaaagtga tcagatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgatcta 181 aatggggtga agcctttgat tttaaaggat tgtagtgtag ctggatggct cctcggaaac 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaat 301 ccagctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagttc ttggcccaat 421 catgaaacat cattaggggt aagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggctcat caaaaagaac aatgcatacc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggaa ttcatcattc caacaatgca 601 gaagagcaga caaatctcta taaaaaccca accacctata tttcagttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgtgga 721 agaatggact tcttctggac aattttaaaa ccgaatgatg caatccattt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt 841 atgaaaagtg gagtggaata tggccactgc aacaccaaat gtcaaacccc agtaggagcg 901 ataaactcta gtatgccgtt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtctttgcg actgggctca gaaatagtcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg aggatggcag 1081 ggaatggttg atggttggta tgggtaccac catagcaatg agcaggggag tgggtacgct 1141 gcagacaaag aatccaccca aaaggcaata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt acgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg aattctatca caaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatagg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc caatgggtcg 1681 ttacagtgca gaatttgcat ttaaatttgt gagctcagat tgtagttaaa aacacccttg 1741 tttctact

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176519 A/chicken/Israel/1048/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1159809 A/Anser cygnoides/Hubei/FW44/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI1159825 A/Cygnus atratus/Hubei/HF-1/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3077.6 0.000000e+00 1690/1702 (99%) EPI1021106 A/mute swan/Czech Republic/1296-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1006681 A/common teal/Korea/W549/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1006680 A/common teal/Korea/W547/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI931207 A/turkey/Germany-BB/R234ff/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI869936 A/chicken/Kalmykia/2661/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI836614 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI836606 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774394 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774386 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774310 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774242 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774234 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774121 A/Bar-headed Goose/Qinghai/BTY1-LV/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774113 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1176518 A/chicken/Israel/881/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1169147 A/chicken/Rostov-on-Don/1321/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1159817 A/Cygnus atratus/Hubei/2Z2-O/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1006705 A/common teal/Korea/W555/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1006704 A/common teal/Korea/W550/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1006703 A/common teal/Korea/W548/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774475 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774458 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774450 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774402 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774378 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774326 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774318 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774302 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774277 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774267 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774259 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774251 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774226 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774218 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774210 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774201 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774193 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774185 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774176 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774168 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774159 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774150 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI978865 A/mute swan/Germany-TH/R1126/2017 (A/H5N8) segment 4 (HA) 3068.4 0.000000e+00 1689/1704 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 3066.6 0.000000e+00 1689/1704 (99%) EPI1176527 A/turkey/Israel/1076/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032524 A/Mulard_duck/Hungary/60369/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032508 A/Goose/Hungary/17985/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032492 A/Goose/Hungary/17051/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032484 A/Goose/Hungary/15729/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032476 A/Goose/Hungary/17261/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1021135 A/quail/Czech Republic/2063-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1021132 A/goose/Czech Republic/1954-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI964917 A/chicken/Czech Republic/206-17_2/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI962046 A/Duck/Hungary/54738/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI961525 A/chicken/Italy/17VIR1751-3/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI961500 A/shelduck/Italy/17VIR1572-24/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI959539 A/Mute swan/Hungary/6276/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI954655 A/Duck/Hungary/984/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI954639 A/Harris Hawk/Hungary/120/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI952639 A/chicken/Korea/H903/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI930838 A/mute swan/Czech Republic/54-17_2/2017 (H5N8) (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI919636 A/chicken/Czech Republic/585-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI891670 A/greylag goose/Croatia/33/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI887468 A/chicken/Czech Republic/206-17_2/2017(H5N8) (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI881901 A/mute swan/Croatia/15/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI869929 A/mute swan/Poland/108/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI866978 A/duck/Hungary/60441/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%)

LOCUS MF166579 1748 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/chicken/Israel/1048/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166579 VERSION MF166579.1 KEYWORDS . SOURCE Influenza A virus (A/chicken/Israel/1048/2016(H5N8)) ORGANISM Influenza A virus (A/chicken/Israel/1048/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1748 /organism="Influenza A virus (A/chicken/Israel/1048/2016(H5N8))" /mol_type="viral cRNA" /strain="A/chicken/Israel/1048/2016" /serotype="H5N8" /host="chicken" /db_xref="taxon:2005431" /segment="4" /country="Israel" /collection_date="20-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45895.1" /translation="MEKIVLLLAIVGLVKSDPICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNDAYPTIKISYNNTNREDLLILWGIHHSNNAEEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKG DSTIMKSEVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVLATGLR NSPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRIC I" sig_peptide 1..48 /gene="HA" mat_peptide 49..1035 /gene="HA" /product="HA1" mat_peptide 1036..1701 /gene="HA" /product="HA2" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttggccttg ttaaaagtga tccgatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgattta 181 aatggggtga aacctctgat tttaaaggat tgtagtgtag ctggatggct cctcggaaac 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaac 301 cccgctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagttc ttggcccaat 421 catgaaacat cattaggggt gagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggcttat caaaaagaac gatgcatacc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggga ttcatcattc caacaatgca 601 gaagagcaga caaatctcta taaaaaccca accacctata tttcggttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgtgga 721 agaatggact tcttttggac aattttaaaa ccgaatgatg caatccactt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt 841 atgaaaagtg aagtggaata tggccactgc aacaccaaat gtcaaacccc agtaggagcg 901 ataaactcta gtatgccatt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtccttgcg actgggctca gaaatagtcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg aggatggcag 1081 ggaatggttg atggttggta tgggtaccac catagcaatg agcaggggag tgggtacgct 1141 gcagacaaag aatccaccca aaaagcaata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt acgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg agttctatca caaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatagg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc caatgggtcg 1681 ttacagtgca gaatttgcat ttaaatttgt gagctcagat tgtagttaaa aacacccttg 1741 tttctact

http://rense2.gsradio.net/rense/special/rense_021518_hr2.mp3

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176527 A/turkey/Israel/1076/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1176530 A/peregrine falcon/Israel/1086/2016 (A/H5N8) segment 4 (HA) 3142.3 0.000000e+00 1703/1704 (99%) EPI1006681 A/common teal/Korea/W549/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1697/1705 (99%) EPI1006680 A/common teal/Korea/W547/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1697/1705 (99%) EPI1006705 A/common teal/Korea/W555/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1006704 A/common teal/Korea/W550/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1006703 A/common teal/Korea/W548/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI952639 A/chicken/Korea/H903/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI1159809 A/Anser cygnoides/Hubei/FW44/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1034974 A/grey heron/W779/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI954567 A/turkey/Italy/17VIR538-1/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI952782 A/mallard duck/Korea/WA137/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI926613 A/domestic duck/Siberia/103/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI926605 A/domestic duck/Siberia/50K/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI925956 A/gadwall/Chany/97/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774458 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774450 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774402 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774378 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774326 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774318 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774277 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774267 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774226 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774218 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774210 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774201 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774193 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1691/1702 (99%) EPI1021106 A/mute swan/Czech Republic/1296-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1010500 A/green-winged teal/Egypt/877/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI931207 A/turkey/Germany-BB/R234ff/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI881901 A/mute swan/Croatia/15/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI869936 A/chicken/Kalmykia/2661/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI858844 A/painted stork/India/10CA03/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI836614 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI836606 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774490 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774483 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774434 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774410 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774394 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774386 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774369 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774335 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774310 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774294 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774286 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774242 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774234 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774142 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774133 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774121 A/Bar-headed Goose/Qinghai/BTY1-LV/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774113 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1169147 A/chicken/Rostov-on-Don/1321/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1159825 A/Cygnus atratus/Hubei/HF-1/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1010489 A/green-winged teal/Egypt/871/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI964917 A/chicken/Czech Republic/206-17_2/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI887468 A/chicken/Czech Republic/206-17_2/2017(H5N8) (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774475 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774442 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI774352 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774302 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774259 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774251 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774185 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774176 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774168 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774159 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774150 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI978865 A/mute swan/Germany-TH/R1126/2017 (A/H5N8) segment 4 (HA) 3073.9 0.000000e+00 1690/1704 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 3072.1 0.000000e+00 1690/1704 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1032524 A/Mulard_duck/Hungary/60369/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1032508 A/Goose/Hungary/17985/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1032492 A/Goose/Hungary/17051/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1032484 A/Goose/Hungary/15729/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1032476 A/Goose/Hungary/17261/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1021135 A/quail/Czech Republic/2063-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%)

LOCUS MF166578 1748 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/turkey/Israel/1076/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166578 VERSION MF166578.1 KEYWORDS . SOURCE Influenza A virus (A/turkey/Israel/1076/2016(H5N8)) ORGANISM Influenza A virus (A/turkey/Israel/1076/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1748 /organism="Influenza A virus (A/turkey/Israel/1076/2016(H5N8))" /mol_type="viral cRNA" /strain="A/turkey/Israel/1076/2016" /serotype="H5N8" /host="tyrkey" /db_xref="taxon:2005439" /segment="4" /country="Israel" /collection_date="24-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45894.1" /translation="MEKIVLLLAIVSLVESDQICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNDAYPTIKISYNNTNREDLLILWGIHHSNNAEEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKG DSTIMKSEVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVLATGLR NNPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHQCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRIC I" sig_peptide 1..48 /gene="HA" mat_peptide 49..1035 /gene="HA" /product="HA1" mat_peptide 1036..1701 /gene="HA" /product="HA2" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttagccttg ttgaaagtga tcagatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgatcta 181 aatggggtga agcctctgat tttaaaggat tgtagtgtag ctggatggct cctcggaaac 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaac 301 ccagctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagttc ttggcccaat 421 catgaaacat cattaggggt gagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggcttat caaaaagaac gatgcatacc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggga ttcatcattc caacaatgca 601 gaagagcaga caaatctcta taaaaaccca accacctata tttcagttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgggga 721 agaatggact tcttctggac aattttaaaa ccgaatgatg caatccactt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt 841 atgaaaagtg aagtggaata tggccactgc aacaccaaat gtcaaacccc agtgggagcg 901 ataaactcta gtatgccatt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtccttgcg actgggctca gaaataatcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg aggatggcag 1081 ggaatggttg atggttggta tgggtaccat catagcaatg agcagggaag tgggtacgct 1141 gcagacaaag aatccaccca aaaggcaata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt acgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg agttctatca ccaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatagg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc caatgggtcg 1681 ttacagtgca gaatttgcat ttaaatttgt gagctcagat tgtagttaaa aacacccttg 1741 tttctact

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176525 A/great egret/Israel/1088/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1176517 A/turkey/Israel/1045/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI1176528 A/great egret/Israel/1084/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1699/1705 (99%) EPI1176524 A/cormorant/Israel/1035/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1698/1706 (99%) EPI1019630 A/Eur_Wig/NL-Wormer/16016143-002/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1019614 A/Eur_Wig/NL-Walterswald/16015923-003/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1007675 A/peacock/Belgium/1017/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI954607 A/turkey/Italy/17VIR1452-22/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI954575 A/turkey/Italy/17VIR576-11/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1694/1705 (99%) EPI1045611 A/chicken/Rostov-on-Don/44/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019534 A/Eur_Wig/NL-Enumatil-Groningen/16015704-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019526 A/Eur_Wig/NL-Drieborg (Dollard)/16015513-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI909444 A/mute swan/Krasnodar/25/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1019718 A/M_Swan/NL-Roggebotsluis/16014462-019/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019678 A/Grey_Go/NL-Groot-Ammers/16015901-012/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019646 A/Eur_Wig/NL-Zwolle/16015820-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019622 A/Eur_Wig/NL-West Graftdijk/16015746-003/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019606 A/Eur_Wig/NL-Vianen/16015917-006/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019590 A/Eur_Wig/NL-Reeuwijk/16015903-003/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019430 A/Ch/NL-Rhenen/16016141-006/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019406 A/Ch/NL-Boven Leeuwen/16016151-006-010/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1019398 A/Ch/NL-Abbega/X16015736/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1007667 A/chicken/Belgium/807/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI959413 A/Eurasian Wigeon/Netherlands/4/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI942943 A/chicken/Wales/000023/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI922508 A/chicken/Germany-NI/R11406/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI909436 A/goose/Krasnodar/3144/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1693/1706 (99%) EPI1019766 A/T_Dk/NL-Almeerder Zand/16014341-003/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1019478 A/Dk/NL-Kamperveen/16016104-001-005/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1019438 A/Ch/NL-Zoeterwoude/16016484-021-025/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI969254 A/Tufted Duck/Switzerland/V237/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI954583 A/chicken/Italy/17VIR653-12/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI909428 A/turkey/Rostov-on-Don/11/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI909404 A/chicken/Voronezh/18/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI909396 A/long-eared owl/Voronezh/16/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI909388 A/Ural owl/Voronezh/14/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI909380 A/long-eared owl/Voronezh/15/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI888088 A/wigeon/Italy/17VIR57-3/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI861011 A/turkey/Germany-SH/R8595/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI860509 A/tufted duck/Germany-SH/R8444/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI860401 A/tufted_duck/Germany/AR8459-L01988/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI859653 A/tufted_duck/Germany/AR8444-L01986/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1023562 A/Eurasian_Wigeon/Netherlands/11/2016 (A/H5N8) segment 4 (HA) 3073.9 0.000000e+00 1689/1701 (99%) EPI1040223 A/chicken/Italy/17VIR3078/2017 (A/H5N8) segment 4 (HA) 3072.1 0.000000e+00 1691/1705 (99%) EPI909364 A/tufted duck/Denmark/17740-1/2016 (A/H5N8) segment 4 (HA) 3072.1 0.000000e+00 1691/1705 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3072.1 0.000000e+00 1691/1705 (99%) EPI1019878 A/Teal/NL-Ferwert/16015273-013/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019870 A/T_Dk/NL-Zuidoost Beemster/16014148-009/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019862 A/T_Dk/NL-Zuidoost Beemster/16014148-002/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019790 A/T_Dk/NL-Rotterdam/16014155-001/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019782 A/T_Dk/NL-Roggebotsluis/16014462-015/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019774 A/T_Dk/NL-Monnickendam/16013865-006-008/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019758 A/Sea_eagle/NL-Assen/16015398-002/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019750 A/P_falcon/NL-Vrouwenpolder (Zeeland)/16015510-001/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019734 A/Mal/NL-IJsselmuiden/16015448-002/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019726 A/Magpie/NL-Volendam/16014331-002/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019710 A/L-bl-ba-gull/NL-Sovon/16014324-014/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019702 A/Gull10/NL-Marker Wadden/16014466-014/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019670 A/Gr_bk_bd_gull/NL-Slootdorp/16014102-005/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019542 A/Eur_Wig/NL-Ferwert/16015273-002/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019446 A/Crow/NL-Oostwoud/16015372-004/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI1019382 A/Buzzard/NL-Durgerdam/16015100-004/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI990770 A/eurasian wigeon/Germany-NI/AR249-L02143/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI954783 A/Common_tern/Hungary/8187/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI909420 A/chicken/Voronezh/20/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI909412 A/chicken/Voronezh/19/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI863865 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI861224 A/chicken/Germany-SH/R8758/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI860393 A/tufted_duck/Germany/AR8444-L01987/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3066.6 0.000000e+00 1689/1703 (99%) EPI1040225 A/turkey/Italy/17VIR5878-3/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1690/1705 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1690/1705 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1690/1705 (99%)

LOCUS MF166573 1748 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/great egret/Israel/1088/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166573 VERSION MF166573.1 KEYWORDS . SOURCE Influenza A virus (A/great egret/Israel/1088/2016(H5N8)) ORGANISM Influenza A virus (A/great egret/Israel/1088/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1748 /organism="Influenza A virus (A/great egret/Israel/1088/2016(H5N8))" /mol_type="viral cRNA" /strain="A/great egret/Israel/1088/2016" /serotype="H5N8" /host="great egret" /db_xref="taxon:2005435" /segment="4" /country="Israel" /collection_date="25-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45889.1" /translation="MEKIVLLLAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNDAYPTIKISYNNTNREDLLILWGIHHSNNAEEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKG DSTIMKSGVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVLATRLR NSPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESMGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRIC I" sig_peptide 1..48 /gene="HA" mat_peptide 49..1035 /gene="HA" /product="HA1" mat_peptide 1036..1701 /gene="HA" /product="HA2" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttagccttg ttaaaagtga tcagatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgatcta 181 aatggggtga agcctttgat tttaaaggat tgtagtgtag ctggatggct cctcggaaat 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaat 301 ccagctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagttc ttggcccaat 421 catgaaacat cattaggggt aagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggctcat caaaaagaac gatgcatacc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggaa ttcatcattc caacaatgca 601 gaagagcaga caaatctcta taaaaaccca accacctata tttcagttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgtgga 721 agaatggact tcttctggac aattttaaaa ccgaatgatg caatccattt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt 841 atgaaaagtg gagtggaata tggccactgc aacaccaaat gtcaaacccc agtaggagcg 901 ataaactcta gtatgccgtt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtcttggcg actaggctca gaaatagtcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg aggatggcag 1081 ggaatggttg atggttggta tgggtaccac catagcaatg agcaggggag tgggtacgct 1141 gcagacaaag aatccaccca aaaggcaata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt acgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg aattctatca caaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatggg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc caatgggtcg 1681 ttacagtgca gaatttgcat ttaaatttgt gagctcagat tgtagttaaa aacacccttg 1741 tttctact

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176528 A/great egret/Israel/1084/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1176517 A/turkey/Israel/1045/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI1176525 A/great egret/Israel/1088/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1699/1705 (99%) EPI1176524 A/cormorant/Israel/1035/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1699/1705 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019630 A/Eur_Wig/NL-Wormer/16016143-002/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019614 A/Eur_Wig/NL-Walterswald/16015923-003/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1007675 A/peacock/Belgium/1017/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI954607 A/turkey/Italy/17VIR1452-22/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI954575 A/turkey/Italy/17VIR576-11/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3099.8 0.000000e+00 1695/1704 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1045611 A/chicken/Rostov-on-Don/44/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019534 A/Eur_Wig/NL-Enumatil-Groningen/16015704-001/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019526 A/Eur_Wig/NL-Drieborg (Dollard)/16015513-001/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI909444 A/mute swan/Krasnodar/25/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019734 A/Mal/NL-IJsselmuiden/16015448-002/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019718 A/M_Swan/NL-Roggebotsluis/16014462-019/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019678 A/Grey_Go/NL-Groot-Ammers/16015901-012/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019646 A/Eur_Wig/NL-Zwolle/16015820-002/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019622 A/Eur_Wig/NL-West Graftdijk/16015746-003/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019606 A/Eur_Wig/NL-Vianen/16015917-006/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019590 A/Eur_Wig/NL-Reeuwijk/16015903-003/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019430 A/Ch/NL-Rhenen/16016141-006/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019406 A/Ch/NL-Boven Leeuwen/16016151-006-010/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019398 A/Ch/NL-Abbega/X16015736/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1007667 A/chicken/Belgium/807/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI959413 A/Eurasian Wigeon/Netherlands/4/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI942943 A/chicken/Wales/000023/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI922508 A/chicken/Germany-NI/R11406/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909436 A/goose/Krasnodar/3144/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019766 A/T_Dk/NL-Almeerder Zand/16014341-003/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019478 A/Dk/NL-Kamperveen/16016104-001-005/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019438 A/Ch/NL-Zoeterwoude/16016484-021-025/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019422 A/Ch/NL-Hiaure/16016112-001-005/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI969254 A/Tufted Duck/Switzerland/V237/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI954583 A/chicken/Italy/17VIR653-12/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI931145 A/duck/Czech Republic/1467-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909428 A/turkey/Rostov-on-Don/11/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909404 A/chicken/Voronezh/18/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909396 A/long-eared owl/Voronezh/16/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909388 A/Ural owl/Voronezh/14/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI909380 A/long-eared owl/Voronezh/15/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI888088 A/wigeon/Italy/17VIR57-3/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI861011 A/turkey/Germany-SH/R8595/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI860509 A/tufted duck/Germany-SH/R8444/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI860401 A/tufted_duck/Germany/AR8459-L01988/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI859653 A/tufted_duck/Germany/AR8444-L01986/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1023562 A/Eurasian_Wigeon/Netherlands/11/2016 (A/H5N8) segment 4 (HA) 3085.0 0.000000e+00 1690/1700 (99%) EPI1040223 A/chicken/Italy/17VIR3078/2017 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI909364 A/tufted duck/Denmark/17740-1/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI1023581 A/Back-headed_Gull/Netherlands/9/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019878 A/Teal/NL-Ferwert/16015273-013/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019870 A/T_Dk/NL-Zuidoost Beemster/16014148-009/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019862 A/T_Dk/NL-Zuidoost Beemster/16014148-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019790 A/T_Dk/NL-Rotterdam/16014155-001/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019782 A/T_Dk/NL-Roggebotsluis/16014462-015/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019774 A/T_Dk/NL-Monnickendam/16013865-006-008/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019758 A/Sea_eagle/NL-Assen/16015398-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019750 A/P_falcon/NL-Vrouwenpolder (Zeeland)/16015510-001/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019726 A/Magpie/NL-Volendam/16014331-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019710 A/L-bl-ba-gull/NL-Sovon/16014324-014/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019702 A/Gull10/NL-Marker Wadden/16014466-014/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019670 A/Gr_bk_bd_gull/NL-Slootdorp/16014102-005/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019598 A/Eur_Wig/NL-Terschelling/16015692-010/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019574 A/Eur_Wig/NL-Leeuwarden/16015699-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019542 A/Eur_Wig/NL-Ferwert/16015273-002/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019446 A/Crow/NL-Oostwoud/16015372-004/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1019382 A/Buzzard/NL-Durgerdam/16015100-004/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI990770 A/eurasian wigeon/Germany-NI/AR249-L02143/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI959460 A/Eurasian Wigeon/Netherlands/9/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI954783 A/Common_tern/Hungary/8187/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI909420 A/chicken/Voronezh/20/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI909412 A/chicken/Voronezh/19/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI863865 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI861224 A/chicken/Germany-SH/R8758/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%)

LOCUS MF166580 1721 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/great egret/Israel/1084/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166580 VERSION MF166580.1 KEYWORDS . SOURCE Influenza A virus (A/great egret/Israel/1084/2016(H5N8)) ORGANISM Influenza A virus (A/great egret/Israel/1084/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1721) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1721) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1721 /organism="Influenza A virus (A/great egret/Israel/1084/2016(H5N8))" /mol_type="viral cRNA" /strain="A/great egret/Israel/1084/2016" /serotype="H5N8" /host="great egret" /db_xref="taxon:2005434" /segment="4" /country="Israel" /collection_date="25-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45896.1" /translation="MEKIVLLLAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNDAYPTIKISYNNTNREDLLILWGIHHSNNAEEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKG DSTIMKSGVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVLATGLR NSPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRIC I" sig_peptide 1..48 /gene="HA" mat_peptide 49..1035 /gene="HA" /product="HA1" mat_peptide 1036..1701 /gene="HA" /product="HA2" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttagccttg ttaaaagtga tcagatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgatcta 181 aatggggtga agcctttgat tttaaaggat tgtagtgtag ctggatggct cctcggaaat 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaat 301 ccagctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagttc ttggcccaat 421 catgaaacat cattaggggt aagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggctcat caaaaagaac gatgcatacc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggaa ttcatcattc caacaatgca 601 gaagagcaga caaatctcta taaaaaccca accacctata tttcagttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgtgga 721 agaatggact tcttctggac aattttaaaa ccgaatgatg caatccattt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt 841 atgaaaagtg gagtggaata tggccactgc aacaccaaat gtcaaacccc agtaggagcg 901 ataaactcta gtatgccatt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtccttgcg actgggctca gaaatagtcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg aggatggcag 1081 ggaatggttg atggttggta tgggtaccac catagcaatg agcaggggag tgggtacgct 1141 gcagacaaag aatccaccca aaaggcaata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt atgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg aattctatca caaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatagg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc caatgggtcg 1681 ttacagtgca gaatttgcat ttaaatttgg gagctcagat g

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176530 A/peregrine falcon/Israel/1086/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI1176527 A/turkey/Israel/1076/2016 (A/H5N8) segment 4 (HA) 3142.3 0.000000e+00 1703/1704 (99%) EPI1006681 A/common teal/Korea/W549/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1006680 A/common teal/Korea/W547/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1696/1705 (99%) EPI1006705 A/common teal/Korea/W555/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1006704 A/common teal/Korea/W550/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI1006703 A/common teal/Korea/W548/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1695/1705 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI952639 A/chicken/Korea/H903/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI1159809 A/Anser cygnoides/Hubei/FW44/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI1034974 A/grey heron/W779/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI954567 A/turkey/Italy/17VIR538-1/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI952782 A/mallard duck/Korea/WA137/2017 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1693/1705 (99%) EPI926613 A/domestic duck/Siberia/103/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI926605 A/domestic duck/Siberia/50K/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI925956 A/gadwall/Chany/97/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774458 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774450 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774402 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774378 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774326 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774318 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774277 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774267 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774226 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774218 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774210 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774201 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774193 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3077.6 0.000000e+00 1690/1702 (99%) EPI1021106 A/mute swan/Czech Republic/1296-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI1010500 A/green-winged teal/Egypt/877/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI931207 A/turkey/Germany-BB/R234ff/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI881901 A/mute swan/Croatia/15/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI869936 A/chicken/Kalmykia/2661/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI858844 A/painted stork/India/10CA03/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI836614 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI836606 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774490 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774483 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774434 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774410 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774394 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774386 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774369 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774335 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774310 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774294 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774286 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774242 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774234 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774142 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774133 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774121 A/Bar-headed Goose/Qinghai/BTY1-LV/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774113 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1692/1705 (99%) EPI1169147 A/chicken/Rostov-on-Don/1321/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1159825 A/Cygnus atratus/Hubei/HF-1/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI1010489 A/green-winged teal/Egypt/871/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI964917 A/chicken/Czech Republic/206-17_2/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI887468 A/chicken/Czech Republic/206-17_2/2017(H5N8) (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI860519 A/goose/Hungary/55128/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774475 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774442 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI774352 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774302 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774259 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774251 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774185 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774176 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774168 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774159 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774150 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI978865 A/mute swan/Germany-TH/R1126/2017 (A/H5N8) segment 4 (HA) 3068.4 0.000000e+00 1689/1704 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 3066.6 0.000000e+00 1689/1704 (99%) EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032549 A/Mulard_duck/Hungary/59163/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032532 A/Mulard_duck/Hungary/62902/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032524 A/Mulard_duck/Hungary/60369/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032508 A/Goose/Hungary/17985/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032492 A/Goose/Hungary/17051/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032484 A/Goose/Hungary/15729/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1032476 A/Goose/Hungary/17261/2017 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI1021135 A/quail/Czech Republic/2063-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%)

LOCUS MF166577 1748 bp cRNA linear VRL 01-FEB-2018 DEFINITION Influenza A virus (A/peregrine falcon/Israel/1086/2016(H5N8)) segment 4 hemagglutinin (HA) gene, complete cds. ACCESSION MF166577 VERSION MF166577.1 KEYWORDS . SOURCE Influenza A virus (A/peregrine falcon/Israel/1086/2016(H5N8)) ORGANISM Influenza A virus (A/peregrine falcon/Israel/1086/2016(H5N8)) Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Influenzavirus A. REFERENCE 1 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Highly pathogenic avian influenza A virus H5N8 isolated in Israel JOURNAL Unpublished REFERENCE 2 (bases 1 to 1748) AUTHORS Shkoda,I., Lapin,K., Simanov,L. and Lublin,A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2017) Division of Avian Diseases, Kimron Veterinari Institute, P.O.Box 12, Bet Dagan 50250, Israel COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. DNASTAR Lasergene 8 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1748 /organism="Influenza A virus (A/peregrine falcon/Israel/1086/2016(H5N8))" /mol_type="viral cRNA" /strain="A/peregrine falcon/Israel/1086/2016" /serotype="H5N8" /host="peregrine falcon" /db_xref="taxon:2005437" /segment="4" /country="Israel" /collection_date="25-Dec-2016" /note="passage details: E1" gene 1..1704 /gene="HA" CDS 1..1704 /gene="HA" /function="receptor binding and fusion protein" /codon_start=1 /product="hemagglutinin" /protein_id="ASA45893.1" /translation="MEKIVLLLAIVSLVESDQICIGYHANNSTEQVDTIMEKNVTVTH AQDILEKTHNGKLCDLNGVKPLILKDCSVAGWLLGNPMCDEFIRVPEWSYIVERANPA NDLCYPGSLNDYEELKHLLSRINHFEKILIIPKSSWPNHETSLGVSAACPYQGTPSFF RNVVWLIKKNDAYPTIKISYNNTNREDLLILWGIHHSNNAEEQTNLYKNPTTYISVGT STLNQRLVPKIATRSQVNGQRGRMDFFWTILKPNDAIHFESNGNFIAPEYAYKIVKKG DSTIMKSEVEYGHCNTKCQTPVGAINSSMPFHNIHPLTIGECPKYVKSNKLVLATGLR NNPLREKRRKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHQCDNECMESVRNGTYDYPQYS EEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRIC I" sig_peptide 1..48 /gene="HA" mat_peptide 49..1035 /gene="HA" /product="HA1" mat_peptide 1036..1701 /gene="HA" /product="HA2" ORIGIN 1 atggagaaaa tagtgcttct tcttgcaata gttagccttg ttgaaagtga tcagatttgc 61 attggttacc atgcaaacaa ctcgacagag caagttgaca cgataatgga aaagaacgtc 121 actgttacac atgcccaaga catactggaa aaaacacaca acgggaagct ctgcgatcta 181 aatggggtga agcctctgat tttaaaggat tgtagtgtag ctggatggct cctcggaaac 241 ccaatgtgcg acgaattcat cagagtgccg gaatggtctt acatagtgga gagggctaac 301 ccagctaatg acctctgtta cccagggagc ctcaatgact atgaagaact gaaacacctg 361 ttgagcagaa taaatcattt tgagaagatt ctgatcatcc ccaagagttc ttggcccaat 421 catgaaacat cattaggggt gagcgcagct tgtccatacc agggaacgcc ctcctttttc 481 agaaatgtgg tatggcttat caaaaagaac gatgcatacc caacaataaa gataagctac 541 aataatacca atcgggaaga tctcttgata ctgtggggga ttcatcattc caacaatgca 601 gaagagcaga caaatctcta taaaaaccca accacctata tttcagttgg aacatcaaca 661 ttaaaccaga gattggtacc aaaaatagct actagatccc aagtaaacgg gcaacgggga 721 agaatggact tcttctggac aattttaaaa ccgaatgatg caatccactt cgagagtaat 781 ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt 841 atgaaaagtg aagtggaata tggccactgc aacaccaaat gtcaaacccc agtgggagcg 901 ataaactcta gtatgccatt ccacaatata catcctctca ccatcgggga atgccccaaa 961 tacgtgaagt caaacaagtt ggtccttgcg actgggctca gaaataatcc tctaagagaa 1021 aagagaagaa aaagagggct gtttggggct atagcaggtt ttatagaggg aggatggcag 1081 ggaatggttg atggttggta tgggtaccat catagcaatg agcagggaag tgggtacgct 1141 gcagacaaag aatccaccca aaaggcgata gatggagtta ccaataaggt caactcgatc 1201 attgacaaaa tgaacactca atttgaggca gttggaaggg agtttaataa cttagaaagg 1261 aggatagaga atttgaacaa gaaaatggaa gacggattcc tagatgtctg gacctataat 1321 gctgaacttc tagttctcat ggaaaacgag aggactctag atttccatga ctcaaatgtc 1381 aagaaccttt acgacaaagt cagactgcag cttagggata atgcaaagga gctgggtaac 1441 ggttgtttcg agttctatca ccaatgtgat aatgaatgta tggaaagtgt gagaaatggg 1501 acgtatgact accctcagta ttcagaagaa gcaagattaa aaagagaaga aataagcgga 1561 gtgaaattag aatcaatagg aacttaccaa atactgtcaa tttattcaac agtggcgagt 1621 tccctagcac tggcaatcat ggtggctggt ctatctttat ggatgtgctc caatgggtcg 1681 ttacagtgca gaatttgcat ttaaatttgt gagctcagat tgtagttaaa aacacccttg 1741 tttctact

Descriptions Align Segment-ID Name Score E-Value Identity EPI1176526 A/turkey/Israel/184/2017 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI954877 A/Mallard/Hungary/1574a/2017 (A/H5N8) segment 4 (HA) 3131.2 0.000000e+00 1701/1704 (99%) EPI954861 A/Mallard/Hungary/1574b/2017 (A/H5N8) segment 4 (HA) 3131.2 0.000000e+00 1701/1704 (99%) EPI954559 A/swan/Italy/17VIR537-2/2017 (A/H5N8) segment 4 (HA) 3131.2 0.000000e+00 1701/1704 (99%) EPI1169201 A/chicken/Rostov-on-Don/1598/2017 (A/H5N8) segment 4 (HA) 3125.7 0.000000e+00 1700/1704 (99%) EPI954813 A/Greylag_goose/Hungary/320/2017 (A/H5N8) segment 4 (HA) 3125.7 0.000000e+00 1700/1704 (99%) EPI1019558 A/Eur_Wig/NL-Greonterp/16015653-001/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI1019638 A/Eur_Wig/NL-Zoeterwoude/16015702-010/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI1019582 A/Eur_Wig/NL-Leidschendam/16015697-007/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI1019550 A/Eur_Wig/NL-Gouda/16015824-001/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI1019518 A/Eur_Wig/NL-De Waal (Texel)/16014891-004/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI1019510 A/Eur_Wig/NL-De Waal (Texel)/16014891-003/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI943320 A/pochard_duck/England/SA12_157809/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI931145 A/duck/Czech Republic/1467-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI1176528 A/great egret/Israel/1084/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019734 A/Mal/NL-IJsselmuiden/16015448-002/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019678 A/Grey_Go/NL-Groot-Ammers/16015901-012/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019646 A/Eur_Wig/NL-Zwolle/16015820-002/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019630 A/Eur_Wig/NL-Wormer/16016143-002/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019622 A/Eur_Wig/NL-West Graftdijk/16015746-003/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019614 A/Eur_Wig/NL-Walterswald/16015923-003/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019606 A/Eur_Wig/NL-Vianen/16015917-006/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019590 A/Eur_Wig/NL-Reeuwijk/16015903-003/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019430 A/Ch/NL-Rhenen/16016141-006/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019406 A/Ch/NL-Boven Leeuwen/16016151-006-010/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1019398 A/Ch/NL-Abbega/X16015736/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI1007675 A/peacock/Belgium/1017/2017 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI959460 A/Eurasian Wigeon/Netherlands/9/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI959413 A/Eurasian Wigeon/Netherlands/4/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI954607 A/turkey/Italy/17VIR1452-22/2017 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI954575 A/turkey/Italy/17VIR576-11/2017 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI942943 A/chicken/Wales/000023/2016 (A/H5N8) segment 4 (HA) 3109.0 0.000000e+00 1697/1704 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3105.3 0.000000e+00 1696/1704 (99%) EPI1045611 A/chicken/Rostov-on-Don/44/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019534 A/Eur_Wig/NL-Enumatil-Groningen/16015704-001/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019526 A/Eur_Wig/NL-Drieborg (Dollard)/16015513-001/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019478 A/Dk/NL-Kamperveen/16016104-001-005/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1019438 A/Ch/NL-Zoeterwoude/16016484-021-025/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI990794 A/greylag goose/Germany-NI/AR1395-L02144/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI969347 A/turkey/Germany-BB/R377ff/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI954759 A/Peregrine_falcon/Hungary/4882/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI954583 A/chicken/Italy/17VIR653-12/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI909452 A/wild duck/Tatarstan/3059/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI909444 A/mute swan/Krasnodar/25/2017 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI1023562 A/Eurasian_Wigeon/Netherlands/11/2016 (A/H5N8) segment 4 (HA) 3101.6 0.000000e+00 1693/1700 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3099.8 0.000000e+00 1695/1704 (99%) EPI1176524 A/cormorant/Israel/1035/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1019718 A/M_Swan/NL-Roggebotsluis/16014462-019/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI1007667 A/chicken/Belgium/807/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI990802 A/greylag goose/Germany-NI/AR703-L02138/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI990770 A/eurasian wigeon/Germany-NI/AR249-L02143/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI916604 A/bronze turkey/Czech Republic/1414-17/2017 (H5N8) (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI909436 A/goose/Krasnodar/3144/2017 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3098.0 0.000000e+00 1695/1704 (99%) EPI907346 A/domestic duck/Germany-BB/R681ff/2017 (A/H5N8) segment 4 (HA) 3094.3 0.000000e+00 1693/1702 (99%) EPI1176517 A/turkey/Israel/1045/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1023578 A/Eurasian_Wigeon/Netherlands/23/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1686/1692 (99%) EPI1021106 A/mute swan/Czech Republic/1296-17_1/2017 (H5N8) (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019766 A/T_Dk/NL-Almeerder Zand/16014341-003/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1019422 A/Ch/NL-Hiaure/16016112-001-005/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI969254 A/Tufted Duck/Switzerland/V237/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI961449 A/gadwall/Kurgan/2442/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI942935 A/turkey/England/003778/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI931207 A/turkey/Germany-BB/R234ff/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909428 A/turkey/Rostov-on-Don/11/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909404 A/chicken/Voronezh/18/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909396 A/long-eared owl/Voronezh/16/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909388 A/Ural owl/Voronezh/14/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI909380 A/long-eared owl/Voronezh/15/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI888088 A/wigeon/Italy/17VIR57-3/2017 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI869936 A/chicken/Kalmykia/2661/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI861011 A/turkey/Germany-SH/R8595/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI860401 A/tufted_duck/Germany/AR8459-L01988/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI859653 A/tufted_duck/Germany/AR8444-L01986/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI1040223 A/chicken/Italy/17VIR3078/2017 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI909364 A/tufted duck/Denmark/17740-1/2016 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI1169147 A/chicken/Rostov-on-Don/1321/2017 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI1159825 A/Cygnus atratus/Hubei/HF-1/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1159809 A/Anser cygnoides/Hubei/FW44/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1023581 A/Back-headed_Gull/Netherlands/9/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019878 A/Teal/NL-Ferwert/16015273-013/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019870 A/T_Dk/NL-Zuidoost Beemster/16014148-009/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019862 A/T_Dk/NL-Zuidoost Beemster/16014148-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019790 A/T_Dk/NL-Rotterdam/16014155-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019782 A/T_Dk/NL-Roggebotsluis/16014462-015/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019774 A/T_Dk/NL-Monnickendam/16013865-006-008/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019758 A/Sea_eagle/NL-Assen/16015398-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019750 A/P_falcon/NL-Vrouwenpolder (Zeeland)/16015510-001/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019726 A/Magpie/NL-Volendam/16014331-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019710 A/L-bl-ba-gull/NL-Sovon/16014324-014/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019702 A/Gull10/NL-Marker Wadden/16014466-014/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019670 A/Gr_bk_bd_gull/NL-Slootdorp/16014102-005/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019598 A/Eur_Wig/NL-Terschelling/16015692-010/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019574 A/Eur_Wig/NL-Leeuwarden/16015699-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019542 A/Eur_Wig/NL-Ferwert/16015273-002/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI1019494 A/Dk/NL-Stolwijk/16016291-016-020/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%)