niman

Volume 22, Number 5—May 2016LetterPhylogeny of Zika Virus in Western Hemisphere, 2015Robert S. Lanciotti , Amy J. Lambert, Mark Holodniy, Sonia Saavedra, and Leticia del Carmen Castillo SignorAuthor affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (R.S. Lanciotti, A.J. Lambert); Department of Veterans Affairs, Palo Alto, California, USA (M. Holodniy); VA Caribbean Health Care System, San Juan, Puerto Rico, USA (S. Saavedra); Laboratorio Nacional de Salud Guatemala, Villa Nueva, Guatemala (L. del Carmen Castillo Signor)http://wwwnc.cdc.gov/eid/article/22/5/16-0065_article

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome46504650100%0.099%KU321639.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds46464646100%0.099%KU365778.1Select seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds46464646100%0.099%KJ776791.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds46414641100%0.099%KU365780.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds46414641100%0.099%KU365779.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds46414641100%0.099%KU365777.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds46234623100%0.099%KU312312.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds45384538100%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds45074507100%0.098%EU545988.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds45064506100%0.098%JN860885.1Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds41294129100%0.095%HQ234499.1Select seq gb|KF268950.1|Zika virus isolate ARB7701 polyprotein gene, complete cds34293429100%0.089%KF268950.1Select seq gb|KF268948.1|Zika virus isolate ARB13565 polyprotein gene, complete cds34293429100%0.089%KF268948.1Select seq gb|KU312315.1|Zika virus isolate Z1106027 polyprotein gene, partial cds3407340773%0.099%KU312315.1Select seq gb|KU312314.1|Zika virus isolate Z1106031 polyprotein gene, partial cds3407340773%0.099%KU312314.1Select seq gb|KU312313.1|Zika virus isolate Z1106032 polyprotein gene, partial cds3393339373%0.099%KU312313.1

LOCUS KU646827 2601 bp ss-RNA linear VRL 02-FEB-2016 DEFINITION Zika virus isolate Si323 polyprotein gene, partial cds. ACCESSION KU646827 VERSION KU646827.1 GI:984923537 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Spondweni virus group. REFERENCE 1 (bases 1 to 2601) AUTHORS Camacho,E., Paternina-Gomez,M., Blanco,P.J., Osorio,J.E. and Aliota,M.T. TITLE Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia JOURNAL Emerging Infect. Dis. 22 (2016) In press REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 2601) AUTHORS Camacho,E., Paternina-Gomez,M., Blanco,P.J., Osorio,J.E. and Aliota,M.T. TITLE Direct Submission JOURNAL Submitted (29-JAN-2016) Pathobiological Sciences, University of Wisconsin, 1656 Linden Dr, Madison, WI, USA COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2601 /organism="Zika virus" /mol_type="genomic RNA" /isolate="Si323" /host="Homo sapiens" /db_xref="taxon:64320" /country="Colombia" /collection_date="10-Nov-2015" /note="genotype: Asian" CDS <1..>2601 /codon_start=1 /product="polyprotein" /protein_id="AMC37200.1" /db_xref="GI:984923538" /translation="IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE LVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI EESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTR GPSLRSTTASGRVIEEWCCRECTMPPLSFWAKDGCWYGMEIRPRKEPESNLVRSMVTA GSTDHMDHFSL" mat_peptide <1..1515 /product="envelope" mat_peptide 1516..>2601 /product="NS1" ORIGIN 1 atcaggtgca taggagtcag caatagggac tttgtggaag gtatgtcagg tgggacttgg 61 gttgatgtcg tcttggaaca tggaggttgt gtcaccgtaa tggcacagga caaaccgact 121 gtcgacatag agctggttac aacaacagtc agcaacatgg cggaggtaag atcctactgc 181 tatgaggcat caatatcaga catggcttcg gacagccgct gcccaacaca aggtgaagcc 241 taccttgaca agcaatcaga cactcaatat gtctgcaaaa gaacgttagt ggacagaggc 301 tggggaaatg gatgtggact ttttggcaaa gggagcctgg tgacatgcgc taagtttgca 361 tgctccaaga aaatgaccgg gaagagcatc cagccagaga atctggagta ccggataatg 421 ttgtcagttc atggctccca gcacagtggg atgatcgtta atgacacagg acatgaaact 481 gatgagaata gagcgaaggt tgagataacg cccaattcac caagagccga agccaccctg 541 gggggttttg gaagcctagg acttgattgt gaaccgagga caggccttga cttttcagat 601 ttgtattact tgactatgaa taacaagcac tggttggttc acaaggagtg gttccacgac 661 attccattac cttggcacgc tggggcagac accggaactc cacactggaa caacaaagaa 721 gcactggtag agttcaagga cgcacatgcc aaaaggcaaa ctgtcgtggt tctagggagt 781 caggaaggag cagttcacac ggcccttgct ggagctctgg aggctgagat ggatggtgca 841 aagggaaggc tgtcctctgg ccacttgaaa tgtcgcctga aaatggacaa acttagattg 901 aagggcgtgt catactcctt gtgtaccgca gcgttcacat tcaccaagat cccggctgaa 961 acactgcacg ggacagtcac agtggaggta cagtacgcag ggacagatgg accttgcaag 1021 gttccagctc agatggcggt ggacatgcaa actctgaccc cagttgggag gttgataacc 1081 gctaaccccg taatcactga aagcactgag aactctaaga tgatgctgga acttgatcca 1141 ccatttgggg actcttacat tgtcatagga gtcggggaga agaagatcac ccaccactgg 1201 cacaggagtg gcagcaccat tggaaaagca tttgaagcca ctgtgagagg tgccaagaga 1261 atggcagtct tgggagacac agcctgggac tttggatcag ttggaggcgc tctcaactca 1321 ttgggcaagg gcatccatca aatttttgga gcagctttca aatcattgtt tggaggaatg 1381 tcctggttct cacaaattct cattggaacg ttgctgatgt ggttgggtct gaacacaaag 1441 aatggatcta tttcccttat gtgcttggcc ttagggggag tgttgatctt cttatccaca 1501 gccgtctctg ctgatgtggg gtgctcggtg gacttctcaa agaaggagac gagatgtggt 1561 acaggggtgt tcgtctataa cgacgttgaa gcctggaggg acaggtacaa gtaccatcct 1621 gactcccccc gtagattggc agcagcagtc aagcaagcct gggaagatgg tatctgcggg 1681 atctcctctg tttcaagaat ggaaaacatc atgtggagat cagtagaagg ggagctcaac 1741 gcaatcctgg aagagaatgg agttcaactg acggtcgttg tgggatctgt aaaaaacccc 1801 atgtggagag gtccacagag attgcccgtg cctgtgaacg agctgcccca cggctggaag 1861 gcttggggga aatcgtactt cgtcagagca gcaaagacaa ataacagctt tgtcgtggat 1921 ggtgacacac tgaaggaatg cccactcaaa catagagcat ggaacagctt tcttgtggag 1981 gatcatgggt tcggggtatt tcacactagt gtctggctca aggttagaga agattattca 2041 ttagagtgtg atccagccgt tattggaaca gctgttaagg gaaaggaggc tgtacacagt 2101 gatctaggct actggattga gagtgagaag aatgacacat ggaggctgaa gagggcccat 2161 ctgatcgaga tgaaaacatg tgaatggcca aagtcccaca cattgtggac agatggaata 2221 gaagagagtg atctgatcat acccaagtct ttagctgggc cactcagcca tcacaatacc 2281 agagagggct acaggaccca aatgaaaggg ccatggcaca gtgaagagct tgaaattcgg 2341 tttgaggaat gcccaggcac taaggtccac gtggaggaaa catgtggaac aagaggacca 2401 tctctgagat caaccactgc aagcggaagg gtgatcgagg aatggtgctg cagggagtgc 2461 acaatgcccc cactgtcgtt ctgggctaaa gatggctgtt ggtatggaat ggagataagg 2521 cccaggaaag aaccagaaag caacttagta aggtcaatgg tgactgcagg atcaactgat 2581 cacatggatc acttctccct t

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome46594659100%0.099%KU321639.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds46554655100%0.099%KU365778.1Select seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds46554655100%0.099%KJ776791.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds46504650100%0.099%KU365780.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds46504650100%0.099%KU365779.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds46504650100%0.099%KU365777.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds46324632100%0.099%KU312312.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds45474547100%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds45164516100%0.099%EU545988.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds45154515100%0.099%JN860885.1Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds41294129100%0.095%HQ234499.1Select seq gb|KU312315.1|Zika virus isolate Z1106027 polyprotein gene, partial cds3420342073%0.099%KU312315.1Select seq gb|KU312314.1|Zika virus isolate Z1106031 polyprotein gene, partial cds3420342073%0.099%KU312314.1Select seq gb|KF268950.1|Zika virus isolate ARB7701 polyprotein gene, complete cds34113411100%0.089%KF268950.1Select seq gb|KF268948.1|Zika virus isolate ARB13565 polyprotein gene, complete cds34113411100%0.089%KF268948.1Select seq gb|KU312313.1|Zika virus isolate Z1106032 polyprotein gene, partial cds3407340773%0.099%KU312313.1

LOCUS KU646828 2601 bp RNA linear VRL 02-FEB-2016 DEFINITION Zika virus isolate Si322 polyprotein gene, partial cds. ACCESSION KU646828 VERSION KU646828.1 GI:984923539 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Spondweni virus group. REFERENCE 1 (bases 1 to 2601) AUTHORS Camacho,E., Paternina-Gomez,M., Blanco,P.J., Osorio,J.E. and Aliota,M.T. TITLE Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia JOURNAL Emerging Infect. Dis. 22 (2016) In press REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 2601) AUTHORS Camacho,E., Paternina-Gomez,M., Blanco,P.J., Osorio,J.E. and Aliota,M.T. TITLE Direct Submission JOURNAL Submitted (29-JAN-2016) Pathobiological Sciences, University of Wisconsin, 1656 Linden Dr, Madison, WI, USA COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..2601 /organism="Zika virus" /mol_type="genomic RNA" /isolate="Si322" /host="Homo sapiens" /db_xref="taxon:64320" /country="Colombia" /collection_date="10-Nov-2015" /note="genotype: Asian" CDS <1..>2601 /codon_start=1 /product="polyprotein" /protein_id="AMC37201.1" /db_xref="GI:984923540" /translation="IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE LVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI EESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTR GPSLRSTTASGRVIEEWCCRECTMPPLSFWAKDGCWYGMEIRPRKEPESNLVRSMVTA GSTDHMDHFSL" mat_peptide <1..1515 /product="envelope" mat_peptide 1516..>2601 /product="NS1" ORIGIN 1 atcaggtgca taggagtcag caatagggac tttgtggaag gtatgtcagg tgggacttgg 61 gttgatgttg tcttggaaca tggaggttgt gtcaccgtaa tggcacagga caaaccgact 121 gtcgacatag agctggttac aacaacagtc agcaacatgg cggaggtaag atcctactgc 181 tatgaggcat caatatcaga catggcttcg gacagccgct gcccaacaca aggtgaagcc 241 taccttgaca agcaatcaga cactcaatat gtctgcaaaa gaacgttagt ggacagaggc 301 tggggaaatg gatgtggact ttttggcaaa gggagcctgg tgacatgcgc taagtttgca 361 tgctccaaga aaatgaccgg gaagagcatc cagccagaga atctggagta ccggataatg 421 ttgtcagttc atggctccca gcacagtggg atgatcgtta atgacacagg acatgaaact 481 gatgagaata gagcgaaggt tgagataacg cccaattcac caagagccga agccaccctg 541 gggggttttg gaagcctagg acttgattgt gaaccgagga caggccttga cttttcagat 601 ttgtattact tgactatgaa taacaagcac tggttggttc acaaggagtg gttccacgac 661 attccattac cttggcacgc tggggcagac accggaactc cacactggaa caacaaagaa 721 gcactggtag agttcaagga cgcacatgcc aaaaggcaaa ctgtcgtggt tctagggagt 781 caagaaggag cagttcacac ggcccttgct ggagctctgg aggctgagat ggatggtgca 841 aagggaaggc tgtcctctgg ccacttgaaa tgtcgcctga aaatggataa acttagattg 901 aagggcgtgt catactcctt gtgtaccgca gcgttcacat tcaccaagat cccggctgaa 961 acactgcacg ggacagtcac agtggaggta cagtacgcag ggacagatgg accttgcaag 1021 gttccagctc agatggcggt ggacatgcaa actctgaccc cagttgggag gttgataacc 1081 gctaaccccg taatcactga aagcactgag aactctaaga tgatgctgga acttgatcca 1141 ccatttgggg actcttacat tgtcatagga gtcggggaga agaagatcac ccaccactgg 1201 cacaggagtg gcagcaccat tggaaaagca tttgaagcca ctgtgagagg tgccaagaga 1261 atggcagtct tgggagacac agcctgggac tttggatcag ttggaggcgc tctcaactca 1321 ttgggcaagg gcatccatca aatttttgga gcagctttca aatcattgtt tggaggaatg 1381 tcctggttct cacaaattct cattggaacg ttgctgatgt ggttgggtct gaacacaaag 1441 aatggatcta tttcccttat gtgcttggcc ttagggggag tgttgatctt cttatccaca 1501 gccgtctctg ctgatgtggg gtgctcggtg gacttctcaa agaaggagac gagatgtggt 1561 acaggggtgt tcgtctataa cgacgttgaa gcctggaggg acaggtacaa gtaccatcct 1621 gactcccccc gtagattggc agcagcagtc aagcaagcct gggaagatgg tatctgcggg 1681 atctcctctg tttcaagaat ggaaaacatc atgtggagat cagtagaagg ggagctcaac 1741 gcaatcctgg aagagaatgg agttcaactg acggtcgttg tgggatctgt aaaaaacccc 1801 atgtggagag gtccacagag attgcccgtg cctgtgaacg agctgcccca cggctggaag 1861 gcttggggga aatcgtactt cgtcagagca gcaaagacaa ataacagctt tgtcgtggac 1921 ggtgacacac tgaaggaatg cccactcaaa catagagcat ggaacagctt tcttgtggag 1981 gatcatgggt tcggggtatt tcacactagt gtctggctca aggttagaga agattattca 2041 ttagagtgtg atccagccgt tattggaaca gctgttaagg gaaaggaggc tgtacacagt 2101 gatctaggct actggattga gagtgagaag aatgacacat ggaggctgaa gagggcccat 2161 ctgatcgaga tgaaaacatg tgaatggcca aagtcccaca cattgtggac agatggaata 2221 gaagagagtg atctgatcat acccaagtct ttagctgggc cactcagcca tcacaatacc 2281 agagagggct acaggaccca aatgaaaggg ccatggcaca gtgaagagct tgaaattcgg 2341 tttgaggaat gcccaggcac taaggtccac gtggaggaaa catgtggaac aagaggacca 2401 tctctgagat caaccactgc aagcggaagg gtgatcgagg aatggtgctg cagggagtgc 2461 acaatgcccc cactgtcgtt ctgggctaaa gatggctgtt ggtatggaat ggagataagg 2521 cccaggaaag aaccagaaag caacttagta aggtcaatgg tgactgcagg atcaactgat 2581 cacatggatc acttctccct t

University of Wisconsin has released partial Zika sequences from Noverber 2015 transmission in Sincelejo, Colombia http://www.ncbi.nlm.nih.gov/nuccore/KU646827.1 http://www.ncbi.nlm.nih.gov/nuccore/KU646828.1

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds1839018390100%0.099%KJ776791.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds1838118381100%0.099%KU365779.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome1838118381100%0.099%KU321639.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds1836618366100%0.099%KU365780.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds1836318363100%0.099%KU365777.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds1834818348100%0.099%KU365778.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds1834818348100%0.099%KU312312.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds177571775799%0.098%JN860885.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds177031770398%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds1759417594100%0.098%EU545988.1Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds164241642499%0.095%HQ234499.1

Volume 22, Number 5—May 2016LetterPhylogeny of Zika Virus in Western Hemisphere, 2015Robert S. Lanciotti , Amy J. Lambert, Mark Holodniy, Sonia Saavedra, and Leticia del Carmen Castillo SignorAuthor affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (R.S. Lanciotti, A.J. Lambert); Department of Veterans Affairs, Palo Alto, California, USA (M. Holodniy); VA Caribbean Health Care System, San Juan, Puerto Rico, USA (S. Saavedra); Laboratorio Nacional de Salud Guatemala, Villa Nueva, Guatemala (L. del Carmen Castillo Signor)Main Article Figure Figure. Phylogenetic tree of Zika virus isolates identified from Guatemala and Puerto Rico in December 2015 (indicated in boldface) compared with reference isolates obtained from GenBank. The isolates from Guatemala and Puerto Rico grouped with other Asian genotype viruses. The tree was derived by neighbor-joining methods (bootstrapped 1,000 times) using complete-genome sequences. Location, year identified, and GenBank strain identification for the viruses used in tree construction are shown. Scale bar indicates number of nucleotide substitutions per site. GenBank accession nos.: KU321639 (Brazil 2015 SPH2015), KJ776791 (French Polynesia H/PF/2013), KF383115 (Central African Republic ARB1362), KF383116 (Senegal 1968 ArD7117), KF383117 (Senegal 1997 ArD128000), KF383118 (Senegal 2001 ArD157995), KF383119 (Senegal 2001 ArD158084), KF268948 (CAR 1979 ARB13565), KF268949 (CAR 1980 ARB15076), KF268950 (CAR 1976 ARB7701), EU545988 (Yap 2007), KF993678 (Thailand 2013 PLCal_ZV), JN860885 (Cambodia 2010 FSS13025), HQ234499 (Malaysia 1966 P6-740), HQ234501 (Senegal 1984 ArD41519), HQ234500 (Nigeria 1968 IbH 30656), LC002520 (Uganda 1947 MR766), KU501215 (Puerto Rico PRVABC59), KU501216 (Guatemala 8375), and KU501217 (Guatemala 103344).

LOCUS KU501216 10272 bp RNA linear VRL 01-FEB-2016 DEFINITION Zika virus strain 103344 polyprotein gene, complete cds. ACCESSION KU501216 VERSION KU501216.1 GI:984874583 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Spondweni virus group. REFERENCE 1 (bases 1 to 10272) AUTHORS Lanciotti,R.S., Lambert,A.J., Holodniy,M., Saavedra,S. and del Carmen Castillo Signor,L. TITLE Phylogeny of Zika Virus in Western Hemisphere, 2015 JOURNAL Emerging Infect. Dis. (2016) In press REMARK Publication Status: Available-Online prior to print DOI: 10.3201/eid2205.160065 REFERENCE 2 (bases 1 to 10272) AUTHORS Lanciotti,R.S. and del Carmen Castillo Signor,L. TITLE Direct Submission JOURNAL Submitted (06-JAN-2016) Diagnostic & Reference Laboratory, Arbovirus Diseases Branch, Centers for Disease Control & Prevention, 3150 Rampart Road, Fort Collins, CO 80521, USA COMMENT ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. V7 Sequencing Technology :: IonTorrent ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10272 /organism="Zika virus" /mol_type="genomic RNA" /strain="103344" /host="Homo sapiens" /db_xref="taxon:64320" /country="Guatemala" /collection_date="01-Dec-2015" CDS 1..10272 /note="C-prM/M-E-NS1-NS2a-NS2b-NS3-NS4A-NS4B-NS5" /codon_start=1 /product="polyprotein" /protein_id="AMC13912.1" /db_xref="GI:984874584" /translation="MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPI RMVLAILAFLRFTAIKPSLGLINRWGSVGKKEAMEIIKKFKKDLAAMLRIINARKEKK RRGADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQI MDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVT LPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKV IYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE LVTTTVSNMAEIRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRAPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI EESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTR GPSLRSTTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKEPESNLVRSMVTA GSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLA ILMGATFAEMNTGGDVAHLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQT AISALEGDLMVLINGFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGL ATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSE VLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKD AEVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGAWY VYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMW HVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARN IQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAIT QGRREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKTRLRTVILAP TRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLY IMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEV PERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKT KHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQR RGRIGRNPNKPGDEYLYGGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADK VAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIL EDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTL PGHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFV LMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPEPEKQRSP QDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIGFSMDIDLRPA SAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPL LMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRTAAGIMKNPVVDGIV VTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSP NKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQ MSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKLRWLVERGYLQPYGK VIDLGCGRGGWSYYAATIRKVQEVKGYTKGGPGHEEPVLVQSYGWNIVRLKSGVDVFH MAAEPCDTLLCDIGESSSSPEVEEARTLRVLSMVGDWLERRPGAFCIKVLCPYTSTMM ETLERLQRRYGGGLVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRP VKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG SYEAPTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPD PQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEKEWKTAV EAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGA RFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSCIPGGRMYADDTAGWD TRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQ DQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDR LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHH FNKLHLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRR DLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKT PVTKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST QVRYLGEEGSTPGVL" ORIGIN 1 atgaaaaacc caaaaaagaa atccggagga ttccggattg tcaatatgct aaaacgcgga 61 gtagcccgtg tgagcccctt tgggggcttg aagaggctgc cagccggact tctgctgggt 121 catgggccca tcaggatggt cttggcgatt ctagcctttt tgagattcac ggcaatcaag 181 ccatcactgg gtctcatcaa tagatggggt tcagtgggga aaaaagaggc tatggaaata 241 ataaagaagt tcaagaaaga tctggctgcc atgctgagaa taatcaatgc taggaaggag 301 aagaagagac gaggcgcaga tactagtgtc ggaattgttg gcctcctgct gaccacagct 361 atggcagcgg aggtcactag acgtgggagt gcatactata tgtacttgga cagaaacgat 421 gctggggagg ccatatcttt tccaaccaca ttggggatga ataagtgtta tatacagatc 481 atggatcttg gacacatgtg tgatgccacc atgagctatg aatgccctat gctggatgag 541 ggggtggaac cagatgacgt cgattgttgg tgcaacacga cgtcaacttg ggttgtgtac 601 ggaacctgcc atcacaaaaa aggtgaagca cggagatcta gaagagctgt gacgctcccc 661 tcccattcca ctaggaagct gcaaacgcgg tcgcaaacct ggttggaatc aagagaatac 721 acaaagcact tgattagagt cgaaaattgg atattcagga accctggctt cgcgttagca 781 gcagctgcca tcgcttggct tttgggaagc tcaacgagcc aaaaagtcat atacttggtc 841 atgatactgc tgattgcccc ggcatacagc atcaggtgca taggagtcag caatagggac 901 tttgtggaag gtatgtcagg tgggacttgg gttgatgttg tcttggaaca tggaggttgt 961 gtcaccgtaa tggcacagga caaaccgact gtcgacatag agctggttac aacaacagtc 1021 agcaacatgg cggagataag atcctactgc tatgaggcat caatatcaga catggcttcg 1081 gacagccgct gcccaacaca aggtgaagcc taccttgaca agcaatcaga cactcaatat 1141 gtctgcaaaa gaacgttagt ggacagaggc tggggaaatg gatgtggact ttttggcaaa 1201 gggagcctgg tgacatgcgc taagtttgca tgctccaaga aaatgaccgg gaagagcatc 1261 cagccagaga atctggagta ccggataatg ctgtcagttc atggctccca gcacagtggg 1321 atgatcgtta atgacacagg acatgaaact gatgagaata gagcgaaggt tgagataacg 1381 cccaattcac caagagccga agccaccctg gggggttttg gaagcctagg acttgattgt 1441 gaaccgagga caggccttga cttttcagat ttgtattact tgactatgaa taacaagcac 1501 tggttggttc acaaggagtg gttccacgac attccattac cttggcacgc tggggcagac 1561 accggaactc cacactggaa caacaaagaa gcactggtgg agttcaagga cgcacatgcc 1621 aaaaggcaaa ctgtcgtggt tctagggagt caagaaggag cagttcacac ggcccttgct 1681 ggagctctgg aggctgagat ggatggtgca aagggaaggc tgtcctctgg ccacttgaaa 1741 tgtcgcctga aaatggataa acttagattg aagggcgtgt catactcctt gtgtaccgca 1801 gcgttcacat tcaccaagat cccggctgaa acactgcacg ggacagtcac agtggaggta 1861 cagtacgcag ggacagatgg accttgcaag gttccagctc agatggcggt ggacatgcaa 1921 actctgaccc cagttgggag gttgataacc gctaaccccg taatcactga aagcactgag 1981 aactctaaga tgatgctgga acttgatcca ccatttgggg actcttacat tgtcatagga 2041 gtcggggaga agaagatcac ccaccactgg cacaggagtg gcagcaccat tggaaaagca 2101 tttgaagcca ctgtgagagg tgccaagaga atggcagtct tgggagacac agcctgggac 2161 tttggatcag ttggaggcgc tctcaactca ttgggcaagg gcatccatca aatttttgga 2221 gctgctttca aatcattgtt tggaggaatg tcctggttct cacaaattct cattggaacg 2281 ttgctgatgt ggttgggtct gaacacaaag aatggatcta tttcccttat gtgcttggcc 2341 ttagggggag tgttgatctt cttatccaca gccgtctctg ctgatgtggg gtgctcggtg 2401 gacttctcaa agaaggagac gagatgcggt acaggggtgt tcgtctataa cgacgttgaa 2461 gcctggaggg acaggtacaa gtaccatcct gactcccccc gtagattggc agcagcagtc 2521 aagcaagcct gggaagatgg tatctgcggg atctcctctg tttcaagaat ggaaaacatc 2581 atgtggagat cagtagaagg ggagctcaac gcaatcctgg aagagaatgg agttcaactg 2641 acggtcgttg tgggatctgt aaaaaacccc atgtggagag ctccacagag attgcccgtg 2701 cctgtgaacg agctgcccca cggctggaag gcttggggga aatcgtactt cgtcagagca 2761 gcaaagacaa ataacagctt tgtcgtggat ggtgacacac tgaaggaatg cccactcaaa 2821 catagagcat ggaacagctt tcttgtggag gatcatgggt tcggggtatt tcacactagt 2881 gtctggctca aggttagaga agattattca ttagagtgtg atccagccgt tattggaaca 2941 gctgttaagg gaaaggaggc tgtacacagt gatctaggct actggattga gagtgagaag 3001 aatgacacat ggaggctgaa gagggcccat ctgatcgaga tgaaaacatg tgaatggcca 3061 aagtcccaca cattgtggac agatggaata gaagagagtg atctgatcat acccaagtct 3121 ttagctgggc cactcagcca tcacaatacc agagagggct acaggaccca aatgaaaggg 3181 ccatggcaca gtgaagagct tgaaattcgg tttgaggaat gcccaggcac taaggtccac 3241 gtggaggaaa catgtggaac aagaggacca tctctgagat caaccactgc aagcggaagg 3301 gtgatcgagg aatggtgctg cagggagtgc acaatgcccc cactgtcgtt ccgggctaaa 3361 gatggctgtt ggtatggaat ggagataagg cccaggaaag aaccagaaag caacttagta 3421 aggtcaatgg tgactgcagg atcaactgat cacatggatc acttctccct tggagtgctt 3481 gtgattctgc tcatggtgca ggaagggcta aagaagagaa tgaccacaaa gatcatcata 3541 agcacatcaa tggcagtgct ggtagctatg atcctgggag gattttcaat gagtgacctg 3601 gctaagcttg caattttgat gggtgccacc ttcgcggaaa tgaacactgg aggagatgta 3661 gctcatctgg cgctgatagc ggcattcaaa gtcagaccag cgttgctggt atctttcatc 3721 ttcagagcta attggacacc ccgtgaaagc atgctactgg ccttggcctc gtgtcttttg 3781 caaactgcga tctccgcctt ggaaggcgac ctgatggttc tcatcaatgg ttttgctttg 3841 gcctggttgg caatacgagc gatggttgtt ccacgcactg ataacatcac cttggcaatc 3901 ctggctgctc tgacaccact ggcccggggc acactgcttg tggcgtggag agcaggcctt 3961 gctacttgcg gggggtttat gctcctctct ctgaagggaa aaggcagtgt gaagaagaac 4021 ttaccatttg tcatggccct gggactaacc gctgtgaggc tggtcgaccc catcaacgtg 4081 gtgggactgc tgttgctcac aaggagtggg aagcggagct ggccccctag cgaagtactc 4141 acagctgttg gcctgatatg cgcattggct ggagggttcg ccaaggcaga tatagagatg 4201 gctgggccca tggccgcggt cggtctgcta attgtcagtt acgtggtctc aggaaagagt 4261 gtggacatgt acattgaaag agcaggtgac atcacatggg aaaaagatgc ggaagtcact 4321 ggaaacagtc cccggctcga tgtggcgcta gatgagagtg gtgatttctc cctggtggag 4381 gatgacggtc cccccatgag agagatcata ctcaaggtgg tcctgatgac catctgtggc 4441 atgaacccaa tagccatacc ctttgcagct ggagcgtggt acgtatacgt gaagactgga 4501 aaaaggagtg gtgctctatg ggatgtgcct gctcccaagg aagtaaaaaa gggggagacc 4561 acagatggag tgtacagagt aatgactcgt agactgctag gttcaacaca agttggagtg 4621 ggagttatgc aagagggggt ctttcacact atgtggcacg tcacaaaagg atccgcactg 4681 agaagcggtg aagggagact tgatccatac tggggagatg tcaagcagga tctggtgtca 4741 tactgtggtc catggaagct agatgccgcc tgggacgggc acagcgaggt gcagctcttg 4801 gccgtgcccc ccggagagag agcgaggaac atccagactc tgcccggaat atttaagaca 4861 aaggatgggg acattggagc ggttgcgctg gattacccag caggaacttc aggatctcca 4921 atcctagaca agtgtgggag agtgatagga ctttatggca atggggtcgt gatcaaaaat 4981 gggagttatg ttagtgccat cacccaaggg aggagggagg aagagactcc tgttgagtgc 5041 ttcgagcctt cgatgctgaa gaagaagcag ctaactgtct tagacttaca tcctggagct 5101 gggaaaacca ggagagttct tcctgaaata gtccgtgaag ccataaaaac aagactccgt 5161 actgtgatct tagctccaac cagggttgtc gctgctgaaa tggaggaggc ccttagaggg 5221 cttccagtgc gttatatgac aacagcagtc aatgtcaccc actctggaac agaaatcgtc 5281 gacttaatgt gccatgccac cttcacttca cgtctactac agccaatcag agtccccaac 5341 tataatctgt atattatgga tgaggcccac ttcacagatc cctcaagtat agcagcaaga 5401 ggatacattt caacaagggt tgagatgggc gaggcggctg ccatcttcat gaccgccacg 5461 ccaccaggaa cccgtgacgc atttccggac tccaactcac caattatgga caccgaagtg 5521 gaagtcccag agagagcctg gagctcaggc tttgattggg tgacggatca ttctggaaaa 5581 acagtttggt ttgttccaag cgtgaggaac ggcaatgaga tcgcagcttg tctgacaaag 5641 gctggaaaac gggtcataca gctcagcaga aagacttttg agacagagtt ccagaaaaca 5701 aaacatcaag agtgggactt tgtcgtgaca actgacattt cagagatggg cgccaacttt 5761 aaagctgacc gtgtcataga ttccaggaga tgcctaaagc cggtcatact tgatggcgag 5821 agagtcattc tggctggacc catgcctgtc acacatgcca gcgctgccca gaggaggggg 5881 cgcataggca ggaatcccaa caaacctgga gatgagtatc tgtatggagg tgggtgcgca 5941 gagactgacg aagaccatgc acactggctt gaagcaagaa tgctccttga caatatttac 6001 ctccaagatg gcctcatagc ctcgctctat cgacctgagg ccgacaaagt agcagccatt 6061 gagggagagt tcaagcttag gacggagcaa aggaagacct ttgtggaact catgaaaaga 6121 ggagatcttc ctgtttggct ggcctatcag gttgcatctg ccggaataac ctacacagat 6181 agaagatggt gctttgatgg cacgaccaac aacaccatac tggaagacag tgtgccggca 6241 gaggtgtgga ccagacacgg agagaaaaga gtgctcaaac cgaggtggat ggacgccaga 6301 gtttgttcag atcatgcggc cctgaagtca ttcaaggagt ttgccgctgg gaaaagagga 6361 gcggcttttg gagtgatgga agccttggga acactgccag gacacatgac agagagattc 6421 caggaagcca ttgacaacct cgctgtgctc atgcgggcag agactggaag caggccttac 6481 aaagccgcgg cggcccaatt gccggagacc ctagagacca ttatgctttt ggggttgctg 6541 ggaacagtct cgctgggaat ctttttcgtc ttgatgagga acaagggcat agggaagatg 6601 ggctttggaa tggtgaccct tggggccagt gcatggctca tgtggctctc ggaaattgag 6661 ccagccagaa ttgcatgtgt cctcattgtt gtgttcctat tgctggtggt gctcatacct 6721 gagccagaaa agcaaagatc tccccaggac aaccaaatgg caatcatcat catggtagca 6781 gtaggtcttc tgggcttgat taccgccaat gaactcggat ggttggagag aacaaagagt 6841 gacctaagcc atctaatggg aaggagagag gagggagcaa ccataggatt ctcaatggac 6901 attgacctgc ggccagcctc agcttgggcc atctatgctg ccttgacaac tttcattacc 6961 ccagccgtcc aacatgcagt gaccacttca tacaacaact actccttaat ggcgatggcc 7021 acgcaagctg gagtgttgtt tggtatgggc aaagggatgc cattctacgc atgggacttt 7081 ggagtcccgc tgctaatgat aggttgctac tcacaattaa cacccctgac cctaatagtg 7141 gccatcattt tgctcgtggc gcactacatg tacttgatcc cagggctgca ggcagcagct 7201 gcgcgtgctg cccagaagag aacggcagct ggcatcatga agaaccctgt tgtggatgga 7261 atagtggtga ctgacattga cacaatgaca attgaccccc aagtggagaa aaagatggga 7321 caggtgctac tcatagcagt agccgtctcc agcgccatac tgtcgcggac cgcctggggg 7381 tggggggagg ctggggccct gatcacagcc gcaacttcca ctttgtggga aggctctccg 7441 aacaagtact ggaactcctc tacagccact tcactgtgta acatttttag gggaagttat 7501 ttggctggag cttctctaat ctacacagta acaagaaacg ctggcttggt caagagacgt 7561 gggggtggaa caggagagac cctgggagag aaatggaagg cccgcttgaa ccagatgtcg 7621 gccctggagt tctactccta caaaaagtca ggcatcactg aggtgtgcag agaagaggcc 7681 cgccgcgccc tcaaggacgg tgtggcaacg ggaggccatg ctgtgtcccg aggaagtgca 7741 aagctgagat ggttggtgga gcggggatac ctgcagccct atggaaaggt cattgatctt 7801 ggatgtggca gagggggctg gagttactac gccgccacca tccgcaaagt tcaagaagtg 7861 aaaggataca caaaaggagg ccctggtcat gaagaacccg tgttggtgca aagctatggg 7921 tggaacatag tccgtcttaa gagtggggtg gacgtctttc atatggcggc tgagccgtgt 7981 gacacgttgc tgtgtgacat aggcgagtca tcatctagtc ctgaagtgga agaagcacgg 8041 acgctcagag tcctctccat ggtgggggat tggcttgaaa gaagaccagg agccttttgt 8101 ataaaagtgt tgtgcccata caccagcact atgatggaaa ccctggagcg actgcagcgt 8161 aggtatgggg gaggactggt cagagtgcca ctctcccgca actctacaca tgagatgtac 8221 tgggtctctg gagcgaaaag caacaccata aaaagtgtgt ccaccacgag ccagctcctc 8281 ttggggcgca tggacgggcc taggaggcca gtgaaatatg aggaggatgt gaatctcggc 8341 tctggcacgc gggctgtggt aagctgcgct gaagctccca acatgaagat cattggtaac 8401 cgcattgaaa ggatccgcag tgagcacgcg gaaacgtggt tctttgacga gaaccaccca 8461 tataggacat gggcttacca tggaagctat gaggccccca cacaagggtc agcgtcctct 8521 ctaataaacg gggttgtcag gctcctgtca aaaccctggg atgtggtgac tggagtcaca 8581 ggaatagcca tgaccgacac cacaccgtat ggtcagcaaa gagttttcaa ggaaaaagtg 8641 gacactaggg tgccagaccc ccaagaaggc actcgtcagg ttatgagcat ggtctcttcc 8701 tggttgtgga aagagctagg caaacacaaa cggccacgag tctgtaccaa agaagagttc 8761 atcaacaagg ttcgtagcaa tgcagcatta ggggcaatat ttgaagagga aaaagagtgg 8821 aagactgcag tggaagctgt gaacgatcca aggttctggg ctctagtgga caaggaaaga 8881 gagcaccacc tgagaggaga gtgccagagt tgtgtgtaca acatgatggg aaaaagagaa 8941 aagaaacaag gggaatttgg aaaggccaag ggcagccgcg ccatctggta tatgtggcta 9001 ggggctagat ttctagagtt cgaagccctt ggattcttga acgaggatca ctggatgggg 9061 agagagaact caggaggtgg tgttgaaggg ctgggattac aaagactcgg atatgtccta 9121 gaagagatga gttgcatacc aggaggaagg atgtatgcag atgacactgc tggctgggac 9181 acccgcatca gcaggtttga tctggagaat gaagctctaa tcaccaacca aatggagaaa 9241 gggcacaggg ccttggcatt ggccataatc aagtacacat accaaaacaa agtggtaaag 9301 gtccttagac cagctgaaaa agggaaaaca gttatggaca ttatttcaag acaagaccaa 9361 agggggagcg gacaagttgt cacttacgct cttaacacat ttaccaacct agtggtgcaa 9421 ctcattcgga atatggaggc tgaggaagtt ctagagatgc aagacttgtg gctgctgcgg 9481 aggtcagaga aagtgaccaa ctggttgcag agcaacggat gggataggct caaacgaatg 9541 gcagtcagtg gagatgattg cgttgtgaag ccaattgatg ataggtttgc acatgccctc 9601 aggttcttga atgatatggg aaaagttagg aaggacacac aagagtggaa accctcaact 9661 ggatgggaca actgggaaga agttccgttt tgctcccacc acttcaacaa gctccatctc 9721 aaggacggga ggtccattgt ggttccctgc cgccaccaag atgaactgat tggccgggcc 9781 cgcgtctctc caggggcggg atggagcatc cgggagactg cttgcctagc aaaatcatat 9841 gcgcaaatgt ggcagctcct ttatttccac agaagggacc tccgactgat ggccaatgcc 9901 atttgttcat ctgtgccagt tgactgggtt ccaactggga gaactacctg gtcaatccat 9961 ggaaagggag aatggatgac cactgaagac atgcttgtgg tgtggaacag agtgtggatt 10021 gaggagaacg accacatgga agacaagacc ccagttacga aatggacaga cattccctat 10081 ttgggaaaaa gggaagactt gtggtgtgga tctctcatag ggcacagacc gcgcaccacc 10141 tgggctgaga acattaaaaa cacagtcaac atggtgcgca ggatcatagg tgatgaagaa 10201 aagtacatgg actacctatc cacccaagtt cgctacttgg gtgaagaagg gtctacacct 10261 ggagtgctgt aa

CDC has release a full Zika sequence from a December 2015 collection from Guatemala outbreak http://www.ncbi.nlm.nih.gov/nuccore/KU501216.1

equences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds1839318393100%0.099%KJ776791.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds1838418384100%0.099%KU365779.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome1838418384100%0.099%KU321639.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds1837218372100%0.099%KU365780.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds1836618366100%0.099%KU365777.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds1835418354100%0.099%KU365778.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds1835418354100%0.099%KU312312.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds177621776299%0.098%JN860885.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds177061770698%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds1759817598100%0.098%EU545988.1Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds164291642999%0.095%HQ234499.1

Volume 22, Number 5—May 2016LetterPhylogeny of Zika Virus in Western Hemisphere, 2015Robert S. Lanciotti , Amy J. Lambert, Mark Holodniy, Sonia Saavedra, and Leticia del Carmen Castillo SignorAuthor affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (R.S. Lanciotti, A.J. Lambert); Department of Veterans Affairs, Palo Alto, California, USA (M. Holodniy); VA Caribbean Health Care System, San Juan, Puerto Rico, USA (S. Saavedra); Laboratorio Nacional de Salud Guatemala, Villa Nueva, Guatemala (L. del Carmen Castillo Signor)Main Article Figure Figure. Phylogenetic tree of Zika virus isolates identified from Guatemala and Puerto Rico in December 2015 (indicated in boldface) compared with reference isolates obtained from GenBank. The isolates from Guatemala and Puerto Rico grouped with other Asian genotype viruses. The tree was derived by neighbor-joining methods (bootstrapped 1,000 times) using complete-genome sequences. Location, year identified, and GenBank strain identification for the viruses used in tree construction are shown. Scale bar indicates number of nucleotide substitutions per site. GenBank accession nos.: KU321639 (Brazil 2015 SPH2015), KJ776791 (French Polynesia H/PF/2013), KF383115 (Central African Republic ARB1362), KF383116 (Senegal 1968 ArD7117), KF383117 (Senegal 1997 ArD128000), KF383118 (Senegal 2001 ArD157995), KF383119 (Senegal 2001 ArD158084), KF268948 (CAR 1979 ARB13565), KF268949 (CAR 1980 ARB15076), KF268950 (CAR 1976 ARB7701), EU545988 (Yap 2007), KF993678 (Thailand 2013 PLCal_ZV), JN860885 (Cambodia 2010 FSS13025), HQ234499 (Malaysia 1966 P6-740), HQ234501 (Senegal 1984 ArD41519), HQ234500 (Nigeria 1968 IbH 30656), LC002520 (Uganda 1947 MR766), KU501215 (Puerto Rico PRVABC59), KU501216 (Guatemala 8375), and KU501217 (Guatemala 103344).

LOCUS KU501217 10272 bp RNA linear VRL 01-FEB-2016 DEFINITION Zika virus strain 8375 polyprotein gene, complete cds. ACCESSION KU501217 VERSION KU501217.1 GI:984874585 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Spondweni virus group. REFERENCE 1 (bases 1 to 10272) AUTHORS Lanciotti,R.S., Lambert,A.J., Holodniy,M., Saavedra,S. and del Carmen Castillo Signor,L. TITLE Phylogeny of Zika Virus in Western Hemisphere, 2015 JOURNAL Emerging Infect. Dis. (2016) In press REMARK Publication Status: Available-Online prior to print DOI: 10.3201/eid2205.160065 REFERENCE 2 (bases 1 to 10272) AUTHORS Lanciotti,R.S. and del Carmen Castillo Signor,L. TITLE Direct Submission JOURNAL Submitted (06-JAN-2016) Diagnostic & Reference Laboratory, Arbovirus Diseases Branch, Centers for Disease Control & Prevention, 3150 Rampart Road, Fort Collins, CO 80521, USA COMMENT ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. V7 Sequencing Technology :: IonTorrent ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10272 /organism="Zika virus" /mol_type="genomic RNA" /strain="8375" /host="Homo sapiens" /db_xref="taxon:64320" /country="Guatemala" /collection_date="01-Nov-2015" CDS 1..10272 /note="C-prM/M-E-NS1-NS2a-NS2b-NS3-NS4A-NS4B-NS5" /codon_start=1 /product="polyprotein" /protein_id="AMC13913.1" /db_xref="GI:984874586" /translation="MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPI RMVLAILAFLRFTAIKPSLGLINRWGSVGKKEAMEIIKKFKKDLAAMLRIINARKEKK RRGADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQI MDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVT LPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKV IYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE LVTTTVSNMAEIRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRAPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI EESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTR GPSLRSTTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKEPESNLVRSMVTA GSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLA ILMGATFAEMNTGGDVAHLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQT AISALEGDLMVLINGFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGL ATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSE VLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKD AEVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGAWY VYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMW HVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARN IQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAIT QGRREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKTRLRTVILAP TRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLY IMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEV PERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKT KHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQR RGRIGRNPNKPGDEYLYGGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADK VAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIL EDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTL PGHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFV LMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPEPEKQRSP QDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIGFSMDIDLRPA SAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPL LMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRTAAGIMKNPVVDGIV VTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSP NKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQ MSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKLRWLVERGYLQPYGK VIDLGCGRGGWSYYAATIRKVQEVKGYTKGGPGHEEPVLVQSYGWNIVRLKSGVDVFH MAAEPCDTLLCDIGESSSSPEVEEARTLRVLSMVGDWLERRPGAFCIKVLCPYTSTMM ETLERLQRRYGGGLVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRP VKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG SYEAPTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPD PQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEKEWKTAV EAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGA RFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSCIPGGRMYADDTAGWD TRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQ DQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDR LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHH FNKLHLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRR DLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKT PVTKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST QVRYLGEEGSTPGVL" ORIGIN 1 atgaaaaacc caaaaaagaa atccggagga ttccggattg tcaatatgct aaaacgcgga 61 gtagcccgtg tgagcccctt tgggggcttg aagaggctgc cagccggact tctgctgggt 121 catgggccca tcaggatggt cttggcgatt ctagcctttt tgagattcac ggcaatcaag 181 ccatcactgg gtctcatcaa tagatggggt tcagtgggga aaaaagaggc tatggaaata 241 ataaagaagt tcaagaaaga tctggctgcc atgctgagaa taatcaatgc taggaaggag 301 aagaagagac gaggcgcaga tactagtgtc ggaattgttg gcctcctgct gaccacagct 361 atggcagcgg aggtcactag acgtgggagt gcatactata tgtacttgga cagaaacgat 421 gctggggagg ccatatcttt tccaaccaca ttggggatga ataagtgtta tatacagatc 481 atggatcttg gacacatgtg tgatgccacc atgagctatg aatgccctat gctggatgag 541 ggggtggaac cagatgacgt cgattgttgg tgcaacacga cgtcaacttg ggttgtgtac 601 ggaacctgcc atcacaaaaa aggtgaagca cggagatcta gaagagctgt gacgctcccc 661 tcccattcca ctaggaagct gcaaacgcgg tcgcaaacct ggttggaatc aagagaatac 721 acaaagcact tgattagagt cgaaaattgg atattcagga accctggctt cgcgttagca 781 gcagctgcca tcgcttggct tttgggaagc tcaacgagcc aaaaagtcat atacttggtc 841 atgatactgc tgattgcccc ggcatacagc atcaggtgca taggagtcag caatagggac 901 tttgtggaag gtatgtcagg tgggacttgg gttgatgttg tcttggaaca tggaggttgt 961 gtcaccgtaa tggcacagga caaaccgact gtcgacatag agctggttac aacaacagtc 1021 agcaacatgg cggagataag atcctactgc tatgaggcat caatatcaga catggcttcg 1081 gacagccgct gcccaacaca aggtgaagcc taccttgaca agcaatcaga cactcaatat 1141 gtctgcaaaa gaacgttagt ggacagaggc tggggaaatg gatgtggact ttttggcaaa 1201 gggagcctgg tgacatgcgc taagtttgca tgctccaaga aaatgaccgg gaagagcatc 1261 cagccagaga atctggagta ccggataatg ctgtcagttc atggctccca gcacagtggg 1321 atgatcgtta atgacacagg acatgaaact gatgagaata gagcgaaggt tgagataacg 1381 cccaattcac caagagccga agccaccctg gggggttttg gaagcctagg acttgattgt 1441 gaaccgagga caggccttga cttttcagat ttgtattact tgactatgaa taacaagcac 1501 tggttggttc acaaggagtg gttccacgac attccattac cttggcacgc tggggcagac 1561 accggaactc cacactggaa caacaaagaa gcactggtgg agttcaagga cgcacatgcc 1621 aaaaggcaaa ctgtcgtggt tctagggagt caagaaggag cagttcacac ggcccttgct 1681 ggagctctgg aggctgagat ggatggtgca aagggaaggc tgtcctctgg ccacttgaaa 1741 tgtcgcctga aaatggataa acttagattg aagggcgtgt catactcctt gtgtaccgca 1801 gcgttcacat tcaccaagat cccggctgaa acactgcacg ggacagtcac agtggaggta 1861 cagtacgcag ggacagatgg accttgcaag gttccagctc agatggcggt ggacatgcaa 1921 actctgaccc cagttgggag gttgataacc gctaaccccg taatcactga aagcactgag 1981 aactctaaga tgatgctgga acttgatcca ccatttgggg actcttacat tgtcatagga 2041 gtcggggaga agaagatcac ccaccactgg cacaggagtg gcagcaccat tggaaaagca 2101 tttgaagcca ctgtgagagg tgccaagaga atggcagtct tgggagacac agcctgggac 2161 tttggatcag ttggaggcgc tctcaactca ttgggcaagg gcatccatca aatttttgga 2221 gctgctttca aatcattgtt tggaggaatg tcctggttct cacaaattct cattggaacg 2281 ttgctgatgt ggttgggtct gaacacaaag aatggatcta tttcccttat gtgcttggcc 2341 ttagggggag tgttgatctt cttatccaca gccgtctctg ctgatgtggg gtgctcggtg 2401 gacttctcaa agaaggagac gagatgcggt acaggggtgt tcgtctataa cgacgttgaa 2461 gcctggaggg acaggtacaa gtaccatcct gactcccccc gtagattggc agcagcagtc 2521 aagcaagcct gggaagatgg tatctgcggg atctcctctg tttcaagaat ggaaaacatc 2581 atgtggagat cagtagaagg ggagctcaac gcaatcctgg aagagaatgg agttcaactg 2641 acggtcgttg tgggatctgt aaaaaacccc atgtggagag ctccacagag attgcccgtg 2701 cctgtgaacg agctgcccca cggctggaag gcttggggga aatcgtactt cgtcagagca 2761 gcaaagacaa ataacagctt tgtcgtggat ggtgacacac tgaaggaatg cccactcaaa 2821 catagagcat ggaacagctt tcttgtggag gatcatgggt tcggggtatt tcacactagt 2881 gtctggctca aggttagaga agattattca ttagagtgtg atccagccgt tattggaaca 2941 gctgttaagg gaaaggaggc tgtacacagt gatctaggct actggattga gagtgagaag 3001 aatgacacat ggaggctgaa gagggcccat ctgatcgaga tgaaaacatg tgaatggcca 3061 aagtcccaca cattgtggac agatggaata gaagagagtg atctgatcat acccaagtct 3121 ttagctgggc cactcagcca tcacaatacc agagagggct acaggaccca aatgaaaggg 3181 ccatggcaca gtgaagagct tgaaattcgg tttgaggaat gcccaggcac taaggtccac 3241 gtggaggaaa catgtggaac aagaggacca tctctgagat caaccactgc aagcggaagg 3301 gtgatcgagg aatggtgctg cagggagtgc acaatgcccc cactgtcgtt ccgggctaaa 3361 gatggctgtt ggtatggaat ggagataagg cccaggaaag aaccagaaag caacttagta 3421 aggtcaatgg tgactgcagg atcaactgat cacatggatc acttctccct tggagtgctt 3481 gtgattctgc tcatggtgca ggaagggcta aagaagagaa tgaccacaaa gatcatcata 3541 agcacatcaa tggcagtgct ggtagctatg atcctgggag gattttcaat gagtgacctg 3601 gctaagcttg caattttgat gggtgccacc ttcgcggaaa tgaacactgg aggagatgta 3661 gctcatctgg cgctgatagc ggcattcaaa gtcagaccag cgttgctggt atctttcatc 3721 ttcagagcta attggacacc ccgtgaaagc atgctactgg ccttggcctc gtgtcttttg 3781 caaactgcga tctccgcctt ggaaggcgac ctgatggttc tcatcaatgg ttttgctttg 3841 gcctggttgg caatacgagc gatggttgtt ccacgcactg ataacatcac cttggcaatc 3901 ctggctgctc tgacaccact ggcccggggc acactgcttg tggcgtggag agcaggcctt 3961 gctacttgcg gggggtttat gctcctctct ctgaagggaa aaggcagtgt gaagaagaac 4021 ttaccatttg tcatggccct gggactaacc gctgtgaggc tggtcgaccc catcaacgtg 4081 gtgggactgc tgttgctcac aaggagtggg aagcggagct ggccccctag cgaagtactc 4141 acagctgttg gcctgatatg cgcattggct ggagggttcg ccaaggcaga tatagagatg 4201 gctgggccca tggccgcggt cggtctgcta attgtcagtt acgtggtctc aggaaagagt 4261 gtggacatgt acattgaaag agcaggtgac atcacatggg aaaaagatgc ggaagtcact 4321 ggaaacagtc cccggctcga tgtggcgcta gatgagagtg gtgatttctc cctggtggag 4381 gatgacggtc cccccatgag agagatcata ctcaaggtgg tcctgatgac catctgtggc 4441 atgaacccaa tagccatacc ctttgcagct ggagcgtggt acgtatacgt gaagactgga 4501 aaaaggagtg gtgctctatg ggatgtgcct gctcccaagg aagtaaaaaa gggggagacc 4561 acagatggag tgtacagagt aatgactcgt agactgctag gttcaacaca agttggagtg 4621 ggagttatgc aagagggggt ctttcacact atgtggcacg tcacaaaagg atccgcactg 4681 agaagcggtg aagggagact tgatccatac tggggagatg tcaagcagga tctggtgtca 4741 tactgtggtc catggaagct agatgccgcc tgggacgggc acagcgaggt gcagctcttg 4801 gccgtgcccc ccggagagag agcgaggaac atccagactc tgcccggaat atttaagaca 4861 aaggatgggg acattggagc ggttgcgctg gattacccag caggaacttc aggatctcca 4921 atcctagaca agtgtgggag agtgatagga ctttatggca atggggtcgt gatcaaaaat 4981 gggagttatg ttagtgccat cacccaaggg aggagggagg aagagactcc tgttgagtgc 5041 ttcgagcctt cgatgctgaa gaagaagcag ctaactgtct tagacttaca tcctggagct 5101 gggaaaacca ggagagttct tcctgaaata gtccgtgaag ccataaaaac aagactccgt 5161 actgtgatct tagctccaac cagggttgtc gctgctgaaa tggaggaggc ccttagaggg 5221 cttccagtgc gttatatgac aacagcagtc aatgtcaccc actctggaac agaaatcgtc 5281 gacttaatgt gccatgccac cttcacttca cgtctactac agccaatcag agtccccaac 5341 tataatctgt atattatgga tgaggcccac ttcacagatc cctcaagtat agcagcaaga 5401 ggatacattt caacaagggt tgagatgggc gaggcggctg ccatcttcat gaccgccacg 5461 ccaccaggaa cccgtgacgc atttccggac tccaactcac caattatgga caccgaagtg 5521 gaagtcccag agagagcctg gagctcaggc tttgattggg tgacggatca ttctggaaaa 5581 acagtttggt ttgttccaag cgtgaggaac ggcaatgaga tcgcagcttg tctgacaaag 5641 gctggaaaac gggtcataca gctcagcaga aagacttttg agacagagtt ccagaaaaca 5701 aaacatcaag agtgggactt tgtcgtgaca actgacattt cagagatggg cgccaacttt 5761 aaagctgacc gtgtcataga ttccaggaga tgcctaaagc cggtcatact tgatggcgag 5821 agagtcattc tggctggacc catgcctgtc acacatgcca gcgctgccca gaggaggggg 5881 cgcataggca ggaatcccaa caaacctgga gatgagtatc tgtatggagg tgggtgcgca 5941 gagactgacg aagaccatgc acactggctt gaagcaagaa tgctccttga caatatttac 6001 ctccaagatg gcctcatagc ctcgctctat cgacctgagg ccgacaaagt agcagccatt 6061 gagggagagt tcaagcttag gacggagcaa aggaagacct ttgtggaact catgaaaaga 6121 ggagatcttc ctgtttggct ggcctatcag gttgcatctg ccggaataac ctacacagat 6181 agaagatggt gctttgatgg cacgaccaac aacaccatac tggaagacag tgtgccggca 6241 gaggtgtgga ccagacacgg agagaaaaga gtgctcaaac cgaggtggat ggacgccaga 6301 gtttgttcag atcatgcggc cctgaagtca ttcaaggagt ttgccgctgg gaaaagagga 6361 gcggcttttg gagtgatgga agccttggga acactgccag gacacatgac agagagattc 6421 caggaagcca ttgacaacct cgctgtgctc atgcgggcag agactggaag caggccttac 6481 aaagccgcgg cggcccaatt gccggagacc ctagagacca ttatgctttt ggggttgctg 6541 ggaacagtct cgctgggaat ctttttcgtc ttgatgagga acaagggcat agggaagatg 6601 ggctttggaa tggtgaccct tggggccagt gcatggctca tgtggctctc ggaaattgag 6661 ccagccagaa ttgcatgtgt cctcattgtt gtgttcctat tgctggtggt gctcatacct 6721 gagccagaaa agcaaagatc tccccaggac aaccaaatgg caatcatcat catggtagca 6781 gtaggtcttc tgggcttgat taccgccaat gaactcggat ggttggagag aacaaagagt 6841 gacctaagcc atctaatggg aaggagagag gagggagcaa ccataggatt ctcaatggac 6901 attgacctgc ggccagcctc agcttgggcc atctatgctg ccttgacaac tttcattacc 6961 ccagccgtcc aacatgcagt gaccacttca tacaacaact actccttaat ggcgatggcc 7021 acgcaagctg gagtgttgtt tggtatgggc aaagggatgc cattctacgc atgggacttt 7081 ggagtcccgc tgctaatgat aggttgctac tcacaattaa cacccctgac cctaatagtg 7141 gccatcattt tgctcgtggc gcactacatg tacttgatcc cagggctgca ggcagcagct 7201 gcgcgtgctg cccagaagag aacggcagct ggcatcatga agaaccctgt tgtggatgga 7261 atagtggtga ctgacattga cacaatgaca attgaccccc aagtggagaa aaagatggga 7321 caggtgctac tcatagcagt agccgtctcc agcgccatac tgtcgcggac cgcctggggg 7381 tggggggagg ctggggccct gatcacagcc gcaacttcca ctttgtggga aggctctccg 7441 aacaagtact ggaactcctc tacagccact tcactgtgta acatttttag gggaagttac 7501 ttggctggag cttctctaat ctacacagta acaagaaacg ctggcttggt caagagacgt 7561 gggggtggaa caggagagac cctgggagag aaatggaagg cccgcttgaa ccagatgtcg 7621 gccctggagt tctactccta caaaaagtca ggcatcactg aggtgtgcag agaagaggcc 7681 cgccgcgccc tcaaggacgg tgtggcaacg ggaggccatg ctgtgtcccg aggaagtgca 7741 aagctgagat ggttggtgga gcggggatac ctgcagccct atggaaaggt cattgatctt 7801 ggatgtggca gagggggctg gagttactac gccgccacca tccgcaaagt tcaagaagtg 7861 aaaggataca caaaaggagg ccctggtcat gaagaacccg tgttggtgca aagctatggg 7921 tggaacatag tccgtcttaa gagtggggtg gacgtctttc atatggcggc tgagccgtgt 7981 gacacgttgc tgtgtgacat aggcgagtca tcatctagtc ctgaagtgga agaagcacgg 8041 acgctcagag tcctctccat ggtgggggat tggcttgaaa gaagaccagg agccttttgt 8101 ataaaagtgt tgtgcccata caccagcact atgatggaaa ccctggagcg actgcagcgt 8161 aggtatgggg gaggactggt cagagtgcca ctctcccgca actctacaca tgagatgtac 8221 tgggtctctg gagcgaaaag caacaccata aaaagtgtgt ccaccacgag ccagctcctc 8281 ttggggcgca tggacgggcc taggaggcca gtgaaatatg aggaggatgt gaatctcggc 8341 tctggcacgc gggctgtggt aagctgcgct gaagctccca acatgaagat cattggtaac 8401 cgcattgaaa ggatccgcag tgagcacgcg gaaacgtggt tctttgacga gaaccaccca 8461 tataggacat gggcttacca tggaagctat gaggccccca cacaagggtc agcgtcctct 8521 ctaataaacg gggttgtcag gctcctgtca aaaccctggg atgtggtgac tggagtcaca 8581 ggaatagcca tgaccgacac cacaccgtat ggtcagcaaa gagttttcaa ggaaaaagtg 8641 gacactaggg tgccagaccc ccaagaaggc actcgtcagg ttatgagcat ggtctcttcc 8701 tggttgtgga aagagctagg caaacacaaa cggccacgag tctgtaccaa agaagagttc 8761 atcaacaagg ttcgtagcaa tgcagcatta ggggcaatat ttgaagagga aaaagagtgg 8821 aagactgcag tggaagctgt gaacgatcca aggttctggg ctctagtgga caaggaaaga 8881 gagcaccacc tgagaggaga gtgccagagt tgtgtgtaca acatgatggg aaaaagagaa 8941 aagaaacaag gggaatttgg aaaggccaag ggcagccgcg ccatctggta tatgtggcta 9001 ggggctagat ttctagagtt cgaagccctt ggattcttga acgaggatca ctggatgggg 9061 agagagaact caggaggtgg tgttgaaggg ctgggattac aaagactcgg atatgtccta 9121 gaagagatga gttgcatacc aggaggaagg atgtatgcag atgacactgc tggctgggac 9181 acccgcatca gcaggtttga tctggagaat gaagctctaa tcaccaacca aatggagaaa 9241 gggcacaggg ccttggcatt ggccataatc aagtacacat accaaaacaa agtggtaaag 9301 gtccttagac cagctgaaaa agggaaaaca gttatggaca ttatttcaag acaagaccaa 9361 agggggagcg gacaagttgt cacttacgct cttaacacat ttaccaacct agtggtgcaa 9421 ctcattcgga atatggaggc tgaggaagtt ctagagatgc aagacttgtg gctgctgcgg 9481 aggtcagaga aagtgaccaa ctggttgcag agcaacggat gggataggct caaacgaatg 9541 gcagtcagtg gagatgattg cgttgtgaag ccaattgatg ataggtttgc acatgccctc 9601 aggttcttga atgatatggg aaaagttagg aaggacacac aagagtggaa accctcaact 9661 ggatgggaca actgggaaga agttccgttt tgctcccacc acttcaacaa gctccatctc 9721 aaggacggga ggtccattgt ggttccctgc cgccaccaag atgaactgat tggccgggcc 9781 cgcgtctctc caggggcggg atggagcatc cgggagactg cttgcctagc aaaatcatat 9841 gcgcaaatgt ggcagctcct ttatttccac agaagggacc tccgactgat ggccaatgcc 9901 atttgttcat ctgtgccagt tgactgggtt ccaactggga gaactacctg gtcaatccat 9961 ggaaagggag aatggatgac cactgaagac atgcttgtgg tgtggaacag agtgtggatt 10021 gaggagaacg accacatgga agacaagacc ccagttacga aatggacaga cattccctat 10081 ttgggaaaaa gggaagactt gtggtgtgga tctctcatag ggcacagacc gcgcaccacc 10141 tgggctgaga acattaaaaa cacagtcaac atggtgcgca ggatcatagg tgatgaagaa 10201 aagtacatgg actacctatc cacccaagtt cgctacttgg gtgaagaagg gtctacacct 10261 ggagtgctgt aa

CDC has released a full Zika sequence from November 2015 Guatemala outbreak http://www.ncbi.nlm.nih.gov/nuccore/KU501217.1

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds191131911399%0.099%KU365778.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds191021910299%0.099%KU365779.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds190931909399%0.099%KU365780.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds190841908499%0.099%KU365777.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome190551905599%0.099%KU321639.1Select seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds189921899299%0.099%KJ776791.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds185831858397%0.099%KU312312.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds177391773996%0.098%JN860885.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds176881768894%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds175851758596%0.098%EU545988.1Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds164241642496%0.095%HQ234499.1

Volume 22, Number 5—May 2016LetterPhylogeny of Zika Virus in Western Hemisphere, 2015Robert S. Lanciotti , Amy J. Lambert, Mark Holodniy, Sonia Saavedra, and Leticia del Carmen Castillo SignorAuthor affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (R.S. Lanciotti, A.J. Lambert); Department of Veterans Affairs, Palo Alto, California, USA (M. Holodniy); VA Caribbean Health Care System, San Juan, Puerto Rico, USA (S. Saavedra); Laboratorio Nacional de Salud Guatemala, Villa Nueva, Guatemala (L. del Carmen Castillo Signor)Main Article Figure Figure. Phylogenetic tree of Zika virus isolates identified from Guatemala and Puerto Rico in December 2015 (indicated in boldface) compared with reference isolates obtained from GenBank. The isolates from Guatemala and Puerto Rico grouped with other Asian genotype viruses. The tree was derived by neighbor-joining methods (bootstrapped 1,000 times) using complete-genome sequences. Location, year identified, and GenBank strain identification for the viruses used in tree construction are shown. Scale bar indicates number of nucleotide substitutions per site. GenBank accession nos.: KU321639 (Brazil 2015 SPH2015), KJ776791 (French Polynesia H/PF/2013), KF383115 (Central African Republic ARB1362), KF383116 (Senegal 1968 ArD7117), KF383117 (Senegal 1997 ArD128000), KF383118 (Senegal 2001 ArD157995), KF383119 (Senegal 2001 ArD158084), KF268948 (CAR 1979 ARB13565), KF268949 (CAR 1980 ARB15076), KF268950 (CAR 1976 ARB7701), EU545988 (Yap 2007), KF993678 (Thailand 2013 PLCal_ZV), JN860885 (Cambodia 2010 FSS13025), HQ234499 (Malaysia 1966 P6-740), HQ234501 (Senegal 1984 ArD41519), HQ234500 (Nigeria 1968 IbH 30656), LC002520 (Uganda 1947 MR766), KU501215 (Puerto Rico PRVABC59), KU501216 (Guatemala 8375), and KU501217 (Guatemala 103344).

LOCUS KU501215 10675 bp RNA linear VRL 01-FEB-2016 DEFINITION Zika virus strain PRVABC59, complete genome. ACCESSION KU501215 VERSION KU501215.1 GI:984874581 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Spondweni virus group. REFERENCE 1 (bases 1 to 10675) AUTHORS Lanciotti,R.S., Lambert,A.J., Holodniy,M., Saavedra,S. and del Carmen Castillo Signor,L. TITLE Phylogeny of Zika Virus in Western Hemisphere, 2015 JOURNAL Emerging Infect. Dis. (2016) In press REMARK Publication Status: Available-Online prior to print DOI: 10.3201/eid2205.160065 REFERENCE 2 (bases 1 to 10675) AUTHORS Lanciotti,R.S. and Holodniy,M. TITLE Direct Submission JOURNAL Submitted (06-JAN-2016) Diagnostic & Reference Laboratory, Arbovirus Diseases Branch, Centers for Disease Control & Prevention, 3150 Rampart Road, Fort Collins, CO 80521, USA COMMENT ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. V7 Sequencing Technology :: IonTorrent ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10675 /organism="Zika virus" /mol_type="genomic RNA" /strain="PRVABC59" /host="Homo sapiens" /db_xref="taxon:64320" /country="Puerto Rico" /collection_date="01-Dec-2015" CDS 107..10378 /note="C-prM/M-E-NS1-NS2a-NS2b-NS3-NS4A-NS4B-NS5" /codon_start=1 /product="polyprotein" /protein_id="AMC13911.1" /db_xref="GI:984874582" /translation="MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPI RMVLAILAFLRFTAIKPSLGLINRWGSVGKKEAMETIKKFKKDLAAMLRIINARKEKK RRGADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQI MDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVT LPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKV IYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE LVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI EESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTR GPSLRSTTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKEPESNLVRSMVTA GSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLA ILMGATFAEMNTGGDVAHLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQT AISALEGDLMVLINGFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGL ATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSE VLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKD AEVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGAWY VYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMW HVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARN IQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAIT QGRREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKTRLRTVILAP TRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLY IMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEV PERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKT KHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQR RGRIGRNPNKPGDEYLYGGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADK VAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIM EDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTL PGHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFV LMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPEPEKQRSP QDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIGFSMDIDLRPA SAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPL LMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRTAAGIMKNPVVDGIV VTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSP NKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQ MSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKLRWLVERGYLQPYGK VIDLGCGRGGWSYYVATIRKVQEVKGYTKGGPGHEEPVLVQSYGWNIVRLKSGVDVFH MAAEPCDTLLCDIGESSSSPEVEEARTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMM ETLERLQRRYGGGLVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRP VKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG SYEAPTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPD PQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEKEWKTAV EAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGA RFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSRIPGGRMYADDTAGWD TRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQ DQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDR LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHH FNKLHLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRR DLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKT PVTKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST QVRYLGEEGSTPGVL" ORIGIN 1 gttgttgatc tgtgtgaatc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 61 gtatcaacag gttttatttt ggatttggaa acgagagttt ctggtcatga aaaacccaaa 121 aaagaaatcc ggaggattcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtgag 181 cccctttggg ggcttgaaga ggctgccagc cggacttctg ctgggtcatg ggcccatcag 241 gatggtcttg gcgattctag cctttttgag attcacggca atcaagccat cactgggtct 301 catcaataga tggggttcag tggggaaaaa agaggctatg gaaacaataa agaagttcaa 361 gaaagatctg gctgccatgc tgagaataat caatgctagg aaggagaaga agagacgagg 421 cgcagatact agtgtcggaa ttgttggcct cctgctgacc acagctatgg cagcggaggt 481 cactagacgt gggagtgcat actatatgta cttggacaga aacgatgctg gggaggccat 541 atcttttcca accacattgg ggatgaataa gtgttatata cagatcatgg atcttggaca 601 catgtgtgat gccaccatga gctatgaatg ccctatgctg gatgaggggg tggaaccaga 661 tgacgtcgat tgttggtgca acacgacgtc aacttgggtt gtgtacggaa cctgccatca 721 caaaaaaggt gaagcacgga gatctagaag agctgtgacg ctcccctccc attccaccag 781 gaagctgcaa acgcggtcgc aaacctggtt ggaatcaaga gaatacacaa agcacttgat 841 tagagtcgaa aattggatat tcaggaaccc tggcttcgcg ttagcagcag ctgccatcgc 901 ttggcttttg ggaagctcaa cgagccaaaa agtcatatac ttggtcatga tactgctgat 961 tgccccggca tacagcatca ggtgcatagg agtcagcaat agggactttg tggaaggtat 1021 gtcaggtggg acttgggttg atgttgtctt ggaacatgga ggttgtgtca ccgtaatggc 1081 acaggacaaa ccgactgtcg acatagagct ggttacaaca acagtcagca acatggcgga 1141 ggtaagatcc tactgctatg aggcatcaat atcagacatg gcttctgaca gccgctgccc 1201 aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac 1261 gttagtggac agaggctggg gaaatggatg tggacttttt ggcaaaggga gcctggtgac 1321 atgcgctaag tttgcatgct ccaagaaaat gaccgggaag agcatccagc cagagaatct 1381 ggagtaccgg ataatgctgt cagttcatgg ctcccagcac agtgggatga tcgttaatga 1441 cacaggacat gaaactgatg agaatagagc gaaagttgag ataacgccca attcaccgag 1501 agccgaagcc accctggggg gttttggaag cctaggactt gattgtgaac cgaggacagg 1561 ccttgacttt tcagatttgt attacttgac tatgaataac aagcactggt tggttcacaa 1621 ggagtggttc cacgacattc cattaccttg gcacgctggg gcagacaccg gaactccaca 1681 ctggaacaac aaagaagcac tggtagagtt caaggacgca catgccaaaa ggcaaactgt 1741 cgtggttcta gggagtcaag aaggagcagt tcacacggcc cttgctggag ctctggaggc 1801 tgagatggat ggtgcaaagg gaaggctgtc ctctggccac ttgaaatgtc gcctgaaaat 1861 ggataaactt agattgaagg gcgtgtcata ctccttgtgt actgcagcgt tcacattcac 1921 caagatcccg gctgaaacac tgcacgggac agtcacagtg gaggtacagt acgcagggac 1981 agatggacct tgcaaggttc cagctcagat ggcggtggac atgcaaactc tgaccccagt 2041 tgggaggttg ataaccgcta accccgtaat cactgaaagc actgagaact ctaagatgat 2101 gctggaactt gatccaccat ttggggactc ttacattgtc ataggagtcg gggagaagaa 2161 gatcacccac cactggcaca ggagtggcag caccattgga aaagcatttg aagccactgt 2221 gagaggtgcc aagagaatgg cagtcttggg agacacagcc tgggactttg gatcagttgg 2281 aggcgctctc aactcattgg gcaagggcat ccatcaaatt tttggagcag ctttcaaatc 2341 attgtttgga ggaatgtcct ggttctcaca aattctcatt ggaacgttgc tgatgtggtt 2401 gggtctgaac acaaagaatg gatctatttc ccttatgtgc ttggccttag ggggagtgtt 2461 gatcttctta tccacagccg tctctgctga tgtggggtgc tcggtggact tctcaaagaa 2521 ggagacgaga tgcggtacag gggtgttcgt ctataacgac gttgaagcct ggagggacag 2581 gtacaagtac catcctgact ccccccgtag attggcagca gcagtcaagc aagcctggga 2641 agatggtatc tgcgggatct cctctgtttc aagaatggaa aacatcatgt ggagatcagt 2701 agaaggggag ctcaacgcaa tcctggaaga gaatggagtt caactgacgg tcgttgtggg 2761 atctgtaaaa aaccccatgt ggagaggtcc acagagattg cccgtgcctg tgaacgagct 2821 gccccacggc tggaaggctt gggggaaatc gtatttcgtc agagcagcaa agacaaataa 2881 cagctttgtc gtggatggtg acacactgaa ggaatgccca ctcaaacata gagcatggaa 2941 cagctttctt gtggaggatc atgggttcgg ggtatttcac actagtgtct ggctcaaggt 3001 tagagaagat tattcattag agtgtgatcc agccgttatt ggaacagctg ttaagggaaa 3061 ggaggctgta cacagtgatc taggctactg gattgagagt gagaagaatg acacatggag 3121 gctgaagagg gcccatctga tcgagatgaa aacatgtgaa tggccaaagt cccacacatt 3181 gtggacagat ggaatagaag agagtgatct gatcataccc aagtctttag ctgggccact 3241 cagccatcac aataccagag agggctacag gacccaaatg aaagggccat ggcacagtga 3301 agagcttgaa attcggtttg aggaatgccc aggcactaag gtccacgtgg aggaaacatg 3361 tggaacaaga ggaccatctc tgagatcaac cactgcaagc ggaagggtga tcgaggaatg 3421 gtgctgcagg gagtgcacaa tgcccccact gtcgttccgg gctaaagatg gctgttggta 3481 tggaatggag ataaggccca ggaaagaacc agaaagcaac ttagtaaggt caatggtgac 3541 tgcaggatca actgatcaca tggaccactt ctcccttgga gtgcttgtga tcctgctcat 3601 ggtgcaggaa gggctgaaga agagaatgac cacaaagatc atcataagca catcaatggc 3661 agtgctggta gctatgatcc tgggaggatt ttcaatgagt gacctggcta agcttgcaat 3721 tttgatgggt gccaccttcg cggaaatgaa cactggagga gatgtagctc atctggcgct 3781 gatagcggca ttcaaagtca gaccagcgtt gctggtatct ttcatcttca gagctaattg 3841 gacaccccgt gaaagcatgc tgctggcctt ggcctcgtgt cttttgcaaa ctgcgatctc 3901 cgccttggaa ggcgacctga tggttctcat caatggtttt gctttggcct ggttggcaat 3961 acgagcgatg gttgttccac gcactgataa catcaccttg gcaatcctgg ctgctctgac 4021 accactggcc cggggcacac tgcttgtggc gtggagagca ggccttgcta cttgcggggg 4081 gtttatgctc ctctctctga agggaaaagg cagtgtgaag aagaacttac catttgtcat 4141 ggccctggga ctaaccgctg tgaggctggt cgaccccatc aacgtggtgg gactgctgtt 4201 gctcacaagg agtgggaagc ggagctggcc ccctagcgaa gtactcacag ctgttggcct 4261 gatatgcgca ttggctggag ggttcgccaa ggcagatata gagatggctg ggcccatggc 4321 cgcggtcggt ctgctaattg tcagttacgt ggtctcagga aagagtgtgg acatgtacat 4381 tgaaagagca ggtgacatca catgggaaaa agatgcggaa gtcactggaa acagtccccg 4441 gctcgatgtg gcgctagatg agagtggtga tttctccctg gtggaggatg acggtccccc 4501 catgagagag atcatactca aggtggtcct gatgaccatc tgtggcatga acccaatagc 4561 catacccttt gcagctggag cgtggtacgt atacgtgaag actggaaaaa ggagtggtgc 4621 tctatgggat gtgcctgctc ccaaggaagt aaaaaagggg gagaccacag atggagtgta 4681 cagagtaatg actcgtagac tgctaggttc aacacaagtt ggagtgggag ttatgcaaga 4741 gggggtcttt cacactatgt ggcacgtcac aaaaggatcc gcgctgagaa gcggtgaagg 4801 gagacttgat ccatactggg gagatgtcaa gcaggatctg gtgtcatact gtggtccatg 4861 gaagctagat gccgcctggg atgggcacag cgaggtgcag ctcttggccg tgccccccgg 4921 agagagagcg aggaacatcc agactctgcc cggaatattt aagacaaagg atggggacat 4981 tggagcggtt gcgctggatt acccagcagg aacttcagga tctccaatcc tagacaagtg 5041 tgggagagtg ataggacttt atggcaatgg ggtcgtgatc aaaaacggga gttatgttag 5101 tgccatcacc caagggagga gggaggaaga gactcctgtt gagtgcttcg agccctcgat 5161 gctgaagaag aagcagctaa ctgtcttaga cttgcatcct ggagctggga aaaccaggag 5221 agttcttcct gaaatagtcc gtgaagccat aaaaacaaga ctccgtactg tgatcttagc 5281 tccaaccagg gttgtcgctg ctgaaatgga ggaggccctt agagggcttc cagtgcgtta 5341 tatgacaaca gcagtcaatg tcacccactc tggaacagaa atcgtcgact taatgtgcca 5401 tgccaccttc acttcacgtc tactacagcc aatcagagtc cccaactata atctgtatat 5461 tatggatgag gcccacttca cagatccctc aagtatagca gcaagaggat acatttcaac 5521 aagggttgag atgggcgagg cggctgccat cttcatgacc gccacgccac caggaacccg 5581 tgacgcattt ccggactcca actcaccaat tatggacacc gaagtggaag tcccagagag 5641 agcctggagc tcaggctttg attgggtgac ggatcattct ggaaaaacag tttggtttgt 5701 tccaagcgtg aggaacggca atgagatcgc agcttgtctg acaaaggctg gaaaacgggt 5761 catacagctc agcagaaaga cttttgagac agagttccag aaaacaaaac atcaagagtg 5821 ggactttgtc gtgacaactg acatttcaga gatgggcgcc aactttaaag ctgaccgtgt 5881 catagattcc aggagatgcc taaagccggt catacttgat ggcgagagag tcattctggc 5941 tggacccatg cctgtcacac atgccagcgc tgcccagagg agggggcgca taggcaggaa 6001 tcccaacaaa cctggagatg agtatctgta tggaggtggg tgcgcagaga ctgacgaaga 6061 ccatgcacac tggcttgaag caagaatgct ccttgacaat atttacctcc aagatggcct 6121 catagcctcg ctctatcgac ctgaggccga caaagtagca gccattgagg gagagttcaa 6181 gcttaggacg gagcaaagga agacctttgt ggaactcatg aaaagaggag atcttcctgt 6241 ttggctggcc tatcaggttg catctgccgg aataacctac acagatagaa gatggtgctt 6301 tgatggcacg accaacaaca ccataatgga agacagtgtg ccggcagagg tgtggaccag 6361 acacggagag aaaagagtgc tcaaaccgag gtggatggac gccagagttt gttcagatca 6421 tgcggccctg aagtcattca aggagtttgc cgctgggaaa agaggagcgg cttttggagt 6481 gatggaagcc ctgggaacac tgccaggaca catgacagag agattccagg aagccattga 6541 caacctcgct gtgctcatgc gggcagagac tggaagcagg ccttacaaag ccgcggcggc 6601 ccaattgccg gagaccctag agaccataat gcttttgggg ttgctgggaa cagtctcgct 6661 gggaatcttc ttcgtcttga tgaggaacaa gggcataggg aagatgggct ttggaatggt 6721 gactcttggg gccagcgcat ggctcatgtg gctctcggaa attgagccag ccagaattgc 6781 atgtgtcctc attgttgtgt tcctattgct ggtggtgctc atacctgagc cagaaaagca 6841 aagatctccc caggacaacc aaatggcaat catcatcatg gtagcagtag gtcttctggg 6901 cttgattacc gccaatgaac tcggatggtt ggagagaaca aagagtgacc taagccatct 6961 aatgggaagg agagaggagg gggcaaccat aggattctca atggacattg acctgcggcc 7021 agcctcagct tgggccatct atgctgcctt gacaactttc attaccccag ccgtccaaca 7081 tgcagtgacc acctcataca acaactactc cttaatggcg atggccacgc aagctggagt 7141 gttgtttggc atgggcaaag ggatgccatt ctacgcatgg gactttggag tcccgctgct 7201 aatgataggt tgctactcac aattaacacc cctgacccta atagtggcca tcattttgct 7261 cgtggcgcac tacatgtact tgatcccagg gctgcaggca gcagctgcgc gtgctgccca 7321 gaagagaacg gcagctggca tcatgaagaa ccctgttgtg gatggaatag tggtgactga 7381 cattgacaca atgacaattg acccccaagt ggagaaaaag atgggacagg tgctactcat 7441 agcagtagcc gtctccagcg ccatactgtc gcggaccgcc tgggggtggg gggaggctgg 7501 ggctctgatc acagccgcaa cttccacttt gtgggaaggc tctccgaaca agtactggaa 7561 ctcctctaca gccacttcac tgtgtaacat ttttagggga agttacttgg ctggagcttc 7621 tctaatctac acagtaacaa gaaacgctgg cttggtcaag agacgtgggg gtggaacagg 7681 agagaccctg ggagagaaat ggaaggcccg cttgaaccag atgtcggccc tggagttcta 7741 ctcctacaaa aagtcaggca tcaccgaggt gtgcagagaa gaggcccgcc gcgccctcaa 7801 ggacggtgtg gcaacgggag gccatgctgt gtcccgagga agtgcaaagc tgagatggtt 7861 ggtggagcgg ggatacctgc agccctatgg aaaggtcatt gatcttggat gtggcagagg 7921 gggctggagt tactacgtcg ccaccatccg caaagttcaa gaagtgaaag gatacacaaa 7981 aggaggccct ggtcatgaag aacccgtgtt ggtgcaaagc tatgggtgga acatagtccg 8041 tcttaagagt ggggtggacg tctttcatat ggcggctgag ccgtgtgaca cgttgctgtg 8101 tgacataggt gagtcatcat ctagtcctga agtggaagaa gcacggacgc tcagagtcct 8161 ctccatggtg ggggattggc ttgaaaaaag accaggagcc ttttgtataa aagtgttgtg 8221 cccatacacc agcactatga tggaaaccct ggagcgactg cagcgtaggt atgggggagg 8281 actggtcaga gtgccactct cccgcaactc tacacatgag atgtactggg tctctggagc 8341 gaaaagcaac accataaaaa gtgtgtccac cacgagccag ctcctcttgg ggcgcatgga 8401 cgggcctagg aggccagtga aatatgagga ggatgtgaat ctcggctctg gcacgcgggc 8461 tgtggtaagc tgcgctgaag ctcccaacat gaagatcatt ggtaaccgca ttgaaaggat 8521 ccgcagtgag cacgcggaaa cgtggttctt tgacgagaac cacccatata ggacatgggc 8581 ttaccatgga agctatgagg cccccacaca agggtcagcg tcctctctaa taaacggggt 8641 tgtcaggctc ctgtcaaaac cctgggatgt ggtgactgga gtcacaggaa tagccatgac 8701 cgacaccaca ccgtatggtc agcaaagagt tttcaaggaa aaagtggaca ctagggtgcc 8761 agacccccaa gaaggcactc gtcaggttat gagcatggtc tcttcctggt tgtggaaaga 8821 gctaggcaaa cacaaacggc cacgagtctg caccaaagaa gagttcatca acaaggttcg 8881 tagcaatgca gcattagggg caatatttga agaggaaaaa gagtggaaga ctgcagtgga 8941 agctgtgaac gatccaaggt tctgggctct agtggacaag gaaagagagc accacctgag 9001 aggagagtgc cagagctgtg tgtacaacat gatgggaaaa agagaaaaga aacaagggga 9061 atttggaaag gccaagggca gccgcgccat ctggtatatg tggctagggg ctagatttct 9121 agagttcgaa gcccttggat tcttgaacga ggatcactgg atggggagag agaactcagg 9181 aggtggtgtt gaagggctgg gattacaaag actcggatat gtcctagaag agatgagtcg 9241 tataccagga ggaaggatgt atgcagatga cactgctggc tgggacaccc gcattagcag 9301 gtttgatctg gagaatgaag ctctaatcac caaccaaatg gagaaagggc acagggcctt 9361 ggcattggcc ataatcaagt acacatacca aaacaaagtg gtaaaggtcc ttagaccagc 9421 tgaaaaaggg aaaacagtta tggacattat ttcgagacaa gaccaaaggg ggagcggaca 9481 agttgtcact tacgctctta acacatttac caacctagtg gtgcaactca ttcggaatat 9541 ggaggctgag gaagttctag agatgcaaga cttgtggctg ctgcggaggt cagagaaagt 9601 gaccaactgg ttgcagagca acggatggga taggctcaaa cgaatggcag tcagtggaga 9661 tgattgcgtt gtgaagccaa ttgatgatag gtttgcacat gccctcaggt tcttgaatga 9721 tatgggaaaa gttaggaagg acacacaaga gtggaaaccc tcaactggat gggacaactg 9781 ggaagaagtt ccgttttgct cccaccactt caacaagctc catctcaagg acgggaggtc 9841 cattgtggtt ccctgccgcc accaagatga actgattggc cgggcccgcg tctctccagg 9901 ggcgggatgg agcatccggg agactgcttg cctagcaaaa tcatatgcgc aaatgtggca 9961 gctcctttat ttccacagaa gggacctccg actgatggcc aatgccattt gttcatctgt 10021 gccagttgac tgggttccaa ctgggagaac tacctggtca atccatggaa agggagaatg 10081 gatgaccact gaagacatgc ttgtggtgtg gaacagagtg tggattgagg agaacgacca 10141 catggaagac aagaccccag ttacgaaatg gacagacatt ccctatttgg gaaaaaggga 10201 agacttgtgg tgtggatctc tcatagggca cagaccgcgc accacctggg ctgagaacat 10261 taaaaacaca gtcaacatgg tgcgcaggat cataggtgat gaagaaaagt acatggacta 10321 cctatccacc caagttcgct acttgggtga agaagggtct acacctggag tgctgtaagc 10381 accaatctta atgttgtcag gcctgctagt cagccacagc ttggggaaag ctgtgcagcc 10441 tgtgaccccc ccaggagaag ctgggaaacc aagcctatag tcaggccgag aacgccatgg 10501 cacggaagaa gccatgctgc ctgtgagccc ctcagaggac actgagtcaa aaaaccccac 10561 gcgcttggag gcgcaggatg ggaaaagaag gtggcgacct tccccaccct tcaatctggg 10621 gcctgaactg gagatcagct gtggatctcc agaagaggga ctagtggtta gagga

CDC has released full Zika sequence from December 2015 Puerto Rico outbreak http://www.ncbi.nlm.nih.gov/nuccore/KU501215.1

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds1901619016100%0.099%KJ776791.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome1900319003100%0.099%KU321639.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds1899018990100%0.099%KU365779.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds1898118981100%0.099%KU365780.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds1897218972100%0.099%KU365777.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds1895418954100%0.099%KU365778.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds185321853297%0.099%KU312312.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds177571775796%0.098%JN860885.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds177241772495%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds176071760796%0.098%EU545988.1Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds164291642996%0.095%HQ234499.1

LOCUS KU647676 10617 bp RNA linear VRL 04-FEB-2016 DEFINITION Zika virus strain MRS_OPY_Martinique_PaRi_2015 polyprotein gene, complete cds. ACCESSION KU647676 VERSION KU647676.1 GI:984915975 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Spondweni virus group. REFERENCE 1 (bases 1 to 10617) AUTHORS Piorkowski,G., Gallian,P., Baronti,C., Richard,P., Leparc Goffart,I. and De Lamballerie,X. TITLE Complete coding sequence of Zika virus from Martinique outbreak 2015 JOURNAL Unpublished REFERENCE 2 (bases 1 to 10617) AUTHORS Piorkowski,G., Gallian,P., Baronti,C., Richard,P., Leparc Goffart,I. and De Lamballerie,X. TITLE Direct Submission JOURNAL Submitted (01-FEB-2016) Umr190 Unite des Virus Emergents, Faculte de Medecine de Marseille, 27 Boulevard Jean Moulin, Marseille 13005, France COMMENT ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 8.5.1 Coverage :: 8570 Sequencing Technology :: IonTorrent ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10617 /organism="Zika virus" /mol_type="genomic RNA" /strain="MRS_OPY_Martinique_PaRi_2015" /host="Homo sapiens" /db_xref="taxon:64320" /lab_host="Vero cells" /country="Martinique" /collection_date="Dec-2015" /note="EVA 1589" CDS 48..10319 /codon_start=1 /product="polyprotein" /protein_id="AMC33116.1" /db_xref="GI:984915976" /translation="MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPI RMVLAILAFLRFTAIKPSLGLINRWGSVGKKEAMEIIKKFKKDLAAMLRIINARKEKK RRGAETSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQI MDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVT LPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKV IYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE LVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI EESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTR GPSLRSTTASGRVIEEWCCRECTMPPLSFWAKDGCWYGMEIRPRKEPESNLVRSMVTA GSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLA ILMGATFAEMNTGGDVAHLALTAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQT AISALEGDLMVLINGFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGL ATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSE VLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKD AEVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGAWY VYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMW HVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARN IQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAIT QGRREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKTRLRTVILAP TRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLY IMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEV PERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKT KHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQR RGRIGRNPNKPGDEYLYGGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADK VAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIM EDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTL PGHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFV LMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPEPEKQRSP QDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIGFSMDIDLRPA SAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPL LMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRTAAGIMKNPVVDGIV VTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSP NKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQ MSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKLRWLVERGYLQPYGK VIDLGCGRGGWSYYAATIRKVQEVKGYTKGGPGHEEPVLVQSYGWNIVRLKSGVDVFH MAAEPCDTLLCDIGESSSSPEVEEARTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMM ETLERLQRRYGGGLVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRP VKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG SYEAPTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPD PQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEKEWKTAV EAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGA RFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSRIPGGRMYADDTAGWD TRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQ DQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDR LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHH FNKLHLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRR DLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKT PVAKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST QVRYLGEEGSTPGVL" ORIGIN 1 agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 61 aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 121 gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca 181 ggatggtctt ggcgattcta gcctttttga gattcacggc aatcaagcca tcactgggtc 241 tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca 301 agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 361 gcgcagaaac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg 421 tcactagacg tgggagtgca tactatatgt acttggacag aaacgatgct ggggaggcca 481 tatcttttcc aaccacattg gggatgaata agtgttatat acagatcatg gatcttggac 541 acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtggaaccag 601 atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc 661 acaaaaaagg tgaagcacgg agatctagaa gagccgtgac gctcccctcc cattccacta 721 ggaagctgca aacgcggtcg caaacctggt tggaatcaag agaatacaca aagcacttga 781 ttagagtcga aaattggata ttcaggaacc ctggtttcgc tttagcagca gctgccatcg 841 cgtggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga 901 ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta 961 tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg 1021 cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1081 aggtaagatc ctactgctat gaggcatcaa tatcagacat ggcttcggac agccgctgcc 1141 caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa 1201 cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1261 catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1321 tggagtaccg gataatgttg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1381 acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1441 gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag 1501 gccttgactt ttcagatttg tattacttga ctatgaataa caagcactgg ttggttcaca 1561 aggagtggtt ccacgacatt ccattacctt ggcacgctgg ggcagacacc ggaactccac 1621 actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg 1681 tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1741 ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1801 tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca 1861 ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1921 cagatggacc ttgcaaggtt ccagcccaga tggcggtgga catgcaaact ctgaccccag 1981 ttgggaggtt gataaccgct aaccccgtaa tcactgaaag cactgagaac tctaagatga 2041 tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2101 agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2161 tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2221 gaggcgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2281 cattgtttgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctgatgtggt 2341 tgggtctgaa cacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt 2401 tgatcttctt atccacagcc gtctctgctg atgtggggtg ctcggtggac ttctcaaaga 2461 aggagacgag atgtggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2521 ggtacaagta ccatcctgac tccccccgta gattggcagc agcagtcaag caagcctggg 2581 aagatggtat ctgcgggatc tcctctgttt caagaatgga aaacatcatg tggagatcag 2641 tagaagggga gctcaacgca atcctggaag agaatggagt tcaactgacg gtcgttgtgg 2701 gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2761 tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2821 acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga 2881 acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg 2941 ttagagaaga ttattcatta gagtgtgatc cagccgttat tggaacagct gttaagggaa 3001 aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaat gacacatgga 3061 ggctgaagag ggcccatctg atcgagatga aaacatgtga atggccaaaa tcccacacat 3121 tgtggacaga tggaatagaa gagagtgatc tgatcatacc caagtcttta gctgggccac 3181 tcagccatca caataccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg 3241 aagagcttga aattcggttt gaggaatgcc caggcactaa ggtccacgtg gaggaaacat 3301 gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3361 ggtgctgcag ggagtgcaca atgcccccac tgtcgttctg ggctaaagat ggctgttggt 3421 atggaatgga gataaggccc aggaaagaac cagaaagcaa cttagtaagg tcaatggtga 3481 ctgcaggatc aactgatcac atggatcact tctcccttgg agtgcttgtg attctgctca 3541 tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg 3601 cagtgctggt agctatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa 3661 ttttgatggg tgccaccttc gcggaaatga acactggagg agatgtagct catctggcgc 3721 tgacagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt 3781 ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct 3841 ctgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3901 tacgagcgat ggttgttcca cgcactgaca acatcacctt ggcaatcctg gctgctctga 3961 caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4021 ggtttatgct cctctctctg aagggaaaag gcagtgtgaa gaagaactta ccatttgtca 4081 tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4141 tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc 4201 tgatatgtgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg 4261 ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4321 ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agtcactgga aacagtcccc 4381 ggctcgacgt ggcgctagat gagagtggtg atttctccct ggtggaggat gacggtcccc 4441 ccatgagaga gatcatactc aaggtggtcc tgatgaccat ctgtggcatg aacccaatag 4501 ccataccctt tgcagctgga gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg 4561 ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4621 acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag 4681 agggggtctt tcacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag 4741 ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat 4801 ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4861 gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca 4921 ttggagcggt tgcgctggat tacccagcag gaacttcagg atctccaatc ctagacaagt 4981 gtgggagagt gataggactt tatggcaatg gggtcgtgat caaaaatggg agttatgtta 5041 gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5101 tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga 5161 gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag 5221 ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt 5281 atatgacaac agcagtcaat gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc 5341 atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata 5401 ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5461 caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc 5521 gtgacgcatt tccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga 5581 gagcctggag ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg 5641 ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5701 tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt 5761 gggactttgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg 5821 tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattctgg 5881 ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga 5941 atcccaataa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgacgaag 6001 accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc 6061 tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca 6121 agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg 6181 tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6241 ttgatggcac gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6301 gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6361 atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag 6421 tgatggaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg 6481 acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6541 cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6601 tgggaatctt tttcgtcttg atgaggaaca agggcatagg gaagatgggc tttggaatgg 6661 tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg 6721 catgtgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6781 aaagatctcc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg 6841 gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc 6901 taatgggaag gagagaggag ggggcaacca taggattctc aatggacatt gacctgcggc 6961 cagcctcagc ttgggccatc tatgctgcct tgacaacttt cattacccca gccgtccaac 7021 atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag 7081 tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccactgc 7141 taatgatagg ttgctactca caattaacac ccctgaccct aatagtggcc atcattttgc 7201 tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc 7261 agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7321 acattgacac aatgacaatt gacccccaag tggagaaaaa gatgggacag gtgctactca 7381 tagcagtagc cgtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg 7441 gggccctgat cacagccgca acttccactt tgtgggaagg ctctccgaac aagtactgga 7501 actcctctac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt 7561 ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag 7621 gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct 7681 actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca 7741 aggacggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt 7801 tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7861 ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa 7921 aaggaggccc tggtcatgaa gaacccgtgt tggtgcaaag ctatgggtgg aacatagtcc 7981 gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt 8041 gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8101 tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt 8161 gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8221 gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8281 cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8341 acgggcctag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8401 ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga 8461 tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8521 cttaccatgg aagctatgag gcccccacac aagggtcagc gtcctctcta ataaacgggg 8581 ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8641 ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc 8701 cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag 8761 agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8821 gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg 8881 aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga 8941 gaggagagtg ccagagttgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9001 aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc 9061 tagagttcga agcccttgga ttcttgaacg aggatcactg gatggggaga gagaactcag 9121 gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9181 gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca 9241 ggtttgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg cacagggcct 9301 tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9361 ctgaaaaagg gaaaacagtt atggacatta tttcgagaca agaccaaagg gggagcggac 9421 aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata 9481 tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag 9541 tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag 9601 atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9661 atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9721 gggaagaagt tccgttttgc tcccaccact tcaacaagct ccatctcaag gacgggaggt 9781 ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgc gtctctccag 9841 gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc 9901 agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg 9961 tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10021 ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc 10081 acatggaaga caagacccca gttgcgaaat ggacagacat tccctatttg ggaaaaaggg 10141 aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca 10201 ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact 10261 acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctgtaag 10321 caccaatctt aatgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10381 ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg 10441 gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10501 cgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10561 ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggag

Faculte de Medecine de Marseille has released a full Zika sequence from a patient in December 2015 Martinique outbreak.http://www.ncbi.nlm.nih.gov/nuccore/KU647676.1

http://rense.gsradio.net:8080/rense/special/rense_020416_hr2.mp3

Zika virus case confirmed in Travis County First Zika case confirmed in Travis County KVUE5:46 p.m. CST February 4, 2016(Photo: Getty Images/iStockPhoto) CONNECTTWEETLINKEDINCOMMENTEMAILMOREAUSTIN – The Austin/Travis County Health and Human Services Department confirmed to KVUE Thursday that a man in Travis County has been diagnosed with the Zika virus. The health department said a man under the age of 50 had been diagnosed with the virus after traveling to Colombia. Zika has been linked to cases of microcephaly in newborns, a condition in which a child’s head is smaller than normal size. The World Health Organization has declared a public health emergency in connection to the outbreak. Most people with the Zika virus don’t show symptoms, making it relatively hard to detect and easy to spread. According to the Centers for Disease Control and Prevention, Zika virus mostly causes three to seven days of mild fever, rash, aches and red eyes. Jeff Taylor, an epidemiologist with the health department told KVUE's Kris Betts that the county is getting one or two potential cases for Zika daily. Taylor added those samples -- 12 so far -- are being sent to the CDC for further testing. The Texas Department of State Health Services told KVUE's Jenni Lee on Tuesday that it was preparing to test for the virus locally instead of sending samples to the CDC. The CDC on Jan. 22 posted interim guidelines for pregnant women, recommending all pregnant women consider postponing travel to areas where Zika is being transmitted. Go here to read the guidelines. http://www.kvue.com/story/news/health/2016/02/04/zika-virus-case-confirmed-travis-county/79836440/

Austin man contracts Zika virus from travel to Colombia 3:25 p.m. Thursday, Feb. 4, 2016 | Filed in: NewsAustin/Travis County health authorities confirmed this afternoon that an Austin man younger than 50 has contracted the Zika virus while traveling in Colombia. He was not hospitalized and recovered at home, said Carole Barasch, a spokeswoman for the Austin/Travis County Health and Human Services Department. The Zika virus is spreading in Latin American and the Caribbean and has been linked to abnormally small heads in newborns and to temporary paralysis. Most of the cases confirmed in the United States, including more than 10 in Texas, have been the result of mosquito bites while traveling to the countries. A case in Dallas this week is believed to have been caused by sexual contact involving a person who did not travel but had sex with someone who did. The aedes aegypti is one of two mosquitoes known to transmit the tropical virus Zika.A type of mosquito in the United States is capable of transmitting the virus, but they have not been detected to date. ……………….. Here’s the press release from the Austin/Travis County Health and Human Services Department The Austin/Travis County Health and Human Services Department has confirmed the first positive case of Zika virus. The person, a male under the age of 50, contracted the illness while travelling to Colombia. The Zika virus has been declared an emerging public health threat by the World Health Organization. The mosquito that carries Zika is endemic to Central Texas. In Brazil, Zika virus infections have been identified in several infants born with microcephaly. On January 22, 2016, the Centers for Disease Control and Prevention (CDC) posted interim guidelines for health care providers in the United States caring for pregnant women during a Zika virus outbreak. CDC is recommending that all pregnant women consider postponing travel to areas where Zika virus transmission is ongoing. A link to the guidance can be found here. The Austin/Travis County Health and Human Services Department had previously alerted local health care providers and partner organizations to be aware of Zika as they see patients and to ask about travel history. The health department works with providers to help them assess the need for laboratory testing and facilitate testing from the Centers for Disease Control as needed. Right now, human testing for Zika in Texas can only be done through the Centers for Disease Control. Within weeks, our state lab in Austin is planning to test for the virus. Mosquito activity is low during our coldest months. Beginning May 1st, the health department’s mosquito management program begins collecting, testing, and treating mosquito breeding areas throughout Austin and Travis County. Zika virus is transmitted to persons primarily through the bite of an infected mosquito (Aedes species). Dallas County received confirmation this week of a patient who developed Zika virus after sexual contact with an infected traveler. This is the only case of locally transmitted Zika virus infection that has been identified in the continental United States at this time. The CDC will issue guidance soon on prevention of sexual transmission of Zika. The continues to encourage people to follow travel precautions and avoid mosquito bites. The most common symptoms of Zika virus disease are fever, rash, joint pain, and conjunctivitis (red eyes). The illness is typically mild and resolves within one week. However, as noted previously, Zika infection in pregnant women may be associated with congenital microcephaly and fetal loss. Guillain-Barre syndrome has also been reported in patients after suspected Zika infection. Preventative measures residents can take to avoid mosquito bites include draining any water around their property, wearing long-sleeved shirts and long pants and using EPA-registered insect repellents. Additional information can be found here. http://www.statesman.com/news/news/austin-man-contracts-zika-virus-from-travel-to-col/nqJ6t/

Map update https://www.google.com/maps/d/edit?hl=en&hl=en&authuser=0&authuser=0&mid=zv94AJqgUct4.kT4qLMXp3SLU