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Additional 2014 Haiti Zika Sequences Support Introduction From Easter Island


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Posted (edited)

University of Florida has released two additional Zika sequences from December 2014 Haiti isolates.  These two partial sequences match each other and the full 2014 Haiti sequence.  All three have A9327G, which is also present in the 2016 Haiti sequence.  This polymorphism is found in earlier sequences from the Asian lineage as well as the French Polynesia sequence and multiple sequences from the 2014 Easter Island outbreak.

Edited by niman
Posted
LOCUS       KX059013                1024 bp    cRNA    linear   VRL 12-APR-2016
DEFINITION  Zika virus isolate Haiti/1227/2014 NS5 gene, partial cds.
ACCESSION   KX059013
VERSION     KX059013.1  GI:1016884666
KEYWORDS    .
SOURCE      Zika virus
  ORGANISM  Zika virus
            Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA
            stage; Flaviviridae; Flavivirus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Lednicky,J.A., Morris,J.G. Jr., Beau De Rochars,V.M., Elbadry,M.A.,
            Okech,B.A. and Loeb,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2016) Environmental and Global Health, University
            of Florida - Gainesville, 1225 Center Drive, Room 4155,
            Gainesville, FL 32610, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Zika virus"
                     /mol_type="viral cRNA"
                     /isolate="Haiti/1227/2014"
                     /isolation_source="plasma"
                     /host="Homo sapiens"
                     /db_xref="taxon:64320"
                     /country="Haiti"
                     /collection_date="15-Dec-2014"
                     /identified_by="John Lednicky"
     CDS             <1..>1024
                     /codon_start=3
                     /product="NS5"
                     /protein_id="AMX81920.1"
                     /db_xref="GI:1016884667"
                     /translation="EFGKAKGSRAIWYMWLGARFLEFEALGFLNEDHWMGRENSGGGV
                     EGLGLQRLGYVLEEMSRIPGGRMYADDTAGWDTRISRFDLENEALITNQMEKGHRALA
                     LAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQDQRGSGQVVTYALNTFTNLVVQLIRN
                     MEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDRLKRMAVSGDDCVVKPIDDRFAHALRF
                     LNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHHFNKLHLKDGRSIVVPCRHQDELIGRA
                     RVSPGAGWSIRETACLAKSYAQMWQLLYFHRRDLRLMANAICSSVPVDWVPTGRTTWS
                     IHGKGE"
ORIGIN      
        1 gggaatttgg aaaggccaag ggcagccgcg ccatctggta tatgtggcta ggggctagat
       61 ttctagagtt cgaagccctt ggattcttga acgaggatca ctggatgggg agagagaact
      121 caggaggtgg tgttgaaggg ctgggattac aaagactcgg atatgtccta gaagagatga
      181 gtcgcatacc aggaggaagg atgtatgcag atgacactgc tggctgggac acccgcatca
      241 gcaggtttga tctggagaat gaagctctaa tcaccaacca aatggagaaa gggcacaggg
      301 ccttggcatt ggccataatc aagtacacat accaaaacaa agtggtaaag gtccttagac
      361 cagctgaaaa agggaagaca gttatggaca ttatttcgag acaagaccaa agggggagcg
      421 gacaagttgt cacttacgct cttaacacat ttaccaacct agtggtgcaa ctcattcgga
      481 atatggaggc tgaggaagtt ctagagatgc aagacttgtg gctgctgcgg aggtcagaga
      541 aagtgaccaa ctggttgcag agcaacggat gggataggct caaacgaatg gcagtcagtg
      601 gagatgattg cgttgtgaag ccaattgatg ataggtttgc acatgccctc aggttcttga
      661 atgatatggg aaaagttagg aaggacacac aagagtggaa accctcaact ggatgggaca
      721 actgggaaga agttccgttt tgctcccacc acttcaacaa gctccatctc aaggacggga
      781 ggtccattgt ggttccctgc cgccaccaag atgaactgat tggccgggcc cgcgtctctc
      841 caggggcggg atggagcatc cgggagactg cttgcctagc aaaatcatat gcgcaaatgt
      901 ggcagctcct ttatttccac agaagggacc tccgactgat ggccaatgcc atttgttcat
      961 ctgtgccagt tgactgggtt ccaactggga gaactacctg gtcaatccat ggaaagggag
     1021 aatg

http://www.ncbi.nlm.nih.gov/nuccore/KX059013.1

 

Posted
 

 

Sequences producing significant alignments:
Select:AllNone Selected:0
Sequences producing significant alignments:
Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccession
18471847100%0.0100%KU509998.2
18471847100%0.0100%KJ776791.1
18421842100%0.099%KU497555.1
18421842100%0.099%KU647676.1
18381838100%0.099%KU870645.1
18381838100%0.099%KU926310.1
18381838100%0.099%KU729217.2
18381838100%0.099%KU820897.1
18381838100%0.099%KU321639.1
18331833100%0.099%KU991811.1
18331833100%0.099%KU926309.1
18331833100%0.099%KU922960.1
18331833100%0.099%KU922923.1
18331833100%0.099%KU853013.1
18331833100%0.099%KU853012.1
18331833100%0.099%KU707826.1
18331833100%0.099%KU527068.1
18331833100%0.099%KU501217.1
18331833100%0.099%KU501216.1
18331833100%0.099%KU501215.1
18331833100%0.099%KU365780.1
18331833100%0.099%KU365779.1
18331833100%0.099%KU365777.1
18291829100%0.099%KU940228.1
18291829100%0.099%KU940224.1
18291829100%0.099%KU820898.1
18291829100%0.099%KU729218.1
18291829100%0.099%KU312312.1
18241824100%0.099%KU963796.1
18241824100%0.099%KU955590.1
18241824100%0.099%KU955589.1
18241824100%0.099%KU740184.2
18241824100%0.099%KU820899.2
18241824100%0.099%KU761564.1
18241824100%0.099%KU744693.1
18241824100%0.099%KU365778.1
18201820100%0.099%KF993678.1
18061806100%0.099%KU866423.1
18061806100%0.099%KU681081.3
17751775100%0.098%KU955593.1
17751775100%0.098%JN860885.1
17751775100%0.098%EU545988.1
1761176195%0.0100%KM078936.1
1757175795%0.099%KM078961.1
1755175595%0.099%KM078930.1
1754175495%0.099%KM078971.1
1754175495%0.099%KM078970.1
1754175495%0.099%KM078933.1
17521752100%0.098%KU681082.3
1752175295%0.099%KM078929.1
1750175096%0.099%KU179098.1
16171617100%0.095%HQ234499.1
1611161187%0.0100%KJ873160.1
1429142977%0.0100%KJ873161.1
1391139177%0.099%KM851039.1
1355135577%0.098%KM851038.1
1353135373%0.099%KU985087.1
1346134673%0.099%KU556802.1
Posted
LOCUS       KX059014                1024 bp    cRNA    linear   VRL 12-APR-2016
DEFINITION  Zika virus isolate Haiti/1230/2014 NS5 gene, partial cds.
ACCESSION   KX059014
VERSION     KX059014.1  GI:1016884668
KEYWORDS    .
SOURCE      Zika virus
  ORGANISM  Zika virus
            Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA
            stage; Flaviviridae; Flavivirus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Lednicky,J.A., Morris,J.G. Jr., Beau De Rochars,V.M., Elbadry,M.A.,
            Okech,B.A. and Loeb,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2016) Environmental and Global Health, University
            of Florida - Gainesville, 1225 Center Drive, Room 4155,
            Gainesville, FL 32610, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Zika virus"
                     /mol_type="viral cRNA"
                     /isolate="Haiti/1230/2014"
                     /isolation_source="plasma"
                     /host="Homo sapiens"
                     /db_xref="taxon:64320"
                     /country="Haiti"
                     /collection_date="17-Dec-2014"
                     /identified_by="John Lednicky"
     CDS             <1..>1024
                     /codon_start=3
                     /product="NS5"
                     /protein_id="AMX81921.1"
                     /db_xref="GI:1016884669"
                     /translation="EFGKAKGSRAIWYMWLGARFLEFEALGFLNEDHWMGRENSGGGV
                     EGLGLQRLGYVLEEMSRIPGGRMYADDTAGWDTRISRFDLENEALITNQMEKGHRALA
                     LAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQDQRGSGQVVTYALNTFTNLVVQLIRN
                     MEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDRLKRMAVSGDDCVVKPIDDRFAHALRF
                     LNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHHFNKLHLKDGRSIVVPCRHQDELIGRA
                     RVSPGAGWSIRETACLAKSYAQMWQLLYFHRRDLRLMANAICSSVPVDWVPTGRTTWS
                     IHGKGE"
ORIGIN      
        1 gggaatttgg aaaggccaag ggcagccgcg ccatctggta tatgtggcta ggggctagat
       61 ttctagagtt cgaagccctt ggattcttga acgaggatca ctggatgggg agagagaact
      121 caggaggtgg tgttgaaggg ctgggattac aaagactcgg atatgtccta gaagagatga
      181 gtcgcatacc aggaggaagg atgtatgcag atgacactgc tggctgggac acccgcatca
      241 gcaggtttga tctggagaat gaagctctaa tcaccaacca aatggagaaa gggcacaggg
      301 ccttggcatt ggccataatc aagtacacat accaaaacaa agtggtaaag gtccttagac
      361 cagctgaaaa agggaagaca gttatggaca ttatttcgag acaagaccaa agggggagcg
      421 gacaagttgt cacttacgct cttaacacat ttaccaacct agtggtgcaa ctcattcgga
      481 atatggaggc tgaggaagtt ctagagatgc aagacttgtg gctgctgcgg aggtcagaga
      541 aagtgaccaa ctggttgcag agcaacggat gggataggct caaacgaatg gcagtcagtg
      601 gagatgattg cgttgtgaag ccaattgatg ataggtttgc acatgccctc aggttcttga
      661 atgatatggg aaaagttagg aaggacacac aagagtggaa accctcaact ggatgggaca
      721 actgggaaga agttccgttt tgctcccacc acttcaacaa gctccatctc aaggacggga
      781 ggtccattgt ggttccctgc cgccaccaag atgaactgat tggccgggcc cgcgtctctc
      841 caggggcggg atggagcatc cgggagactg cttgcctagc aaaatcatat gcgcaaatgt
      901 ggcagctcct ttatttccac agaagggacc tccgactgat ggccaatgcc atttgttcat
      961 ctgtgccagt tgactgggtt ccaactggga gaactacctg gtcaatccat ggaaagggag
     1021 aatg

http://www.ncbi.nlm.nih.gov/nuccore/KX059014.1

 

Posted
Sequences producing significant alignments:

Select:AllNone Selected:0

Sequences producing significant alignments:
Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccession
18471847100%0.0100%KU509998.2
18471847100%0.0100%KJ776791.1
18421842100%0.099%KU497555.1
18421842100%0.099%KU647676.1
18381838100%0.099%KU870645.1
18381838100%0.099%KU926310.1
18381838100%0.099%KU729217.2
18381838100%0.099%KU820897.1
18381838100%0.099%KU321639.1
18331833100%0.099%KU991811.1
18331833100%0.099%KU926309.1
18331833100%0.099%KU922960.1
18331833100%0.099%KU922923.1
18331833100%0.099%KU853013.1
18331833100%0.099%KU853012.1
18331833100%0.099%KU707826.1
18331833100%0.099%KU527068.1
18331833100%0.099%KU501217.1
18331833100%0.099%KU501216.1
18331833100%0.099%KU501215.1
18331833100%0.099%KU365780.1
18331833100%0.099%KU365779.1
18331833100%0.099%KU365777.1
18291829100%0.099%KU940228.1
18291829100%0.099%KU940224.1
18291829100%0.099%KU820898.1
18291829100%0.099%KU729218.1
18291829100%0.099%KU312312.1
18241824100%0.099%KU963796.1
18241824100%0.099%KU955590.1
18241824100%0.099%KU955589.1
18241824100%0.099%KU740184.2
18241824100%0.099%KU820899.2
18241824100%0.099%KU761564.1
18241824100%0.099%KU744693.1
18241824100%0.099%KU365778.1
18201820100%0.099%KF993678.1
18061806100%0.099%KU866423.1
18061806100%0.099%KU681081.3
17751775100%0.098%KU955593.1
17751775100%0.098%JN860885.1
17751775100%0.098%EU545988.1
1761176195%0.0100%KM078936.1
1757175795%0.099%KM078961.1
1755175595%0.099%KM078930.1
1754175495%0.099%KM078971.1
1754175495%0.099%KM078970.1
1754175495%0.099%KM078933.1
17521752100%0.098%KU681082.3
1752175295%0.099%KM078929.1
1750175096%0.099%KU179098.1
16171617100%0.095%HQ234499.1
1611161187%0.0100%KJ873160.1
1429142977%0.0100%KJ873161.1
1391139177%0.099%KM851039.1
1355135577%0.098%KM851038.1
1353135373%0.099%KU985087.1
1346134673%0.099%KU556802.1
Posted
Sequences producing significant alignments:

Select:AllNone Selected:0

Sequences producing significant alignments:
Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccession
18921892100%0.0100%KX059014.1
18921892100%0.0100%KX059013.1
18921892100%0.0100%KX051563.1
18921892100%0.0100%KU509998.3
18921892100%0.0100%KJ776791.1
18861886100%0.099%KU497555.1
18861886100%0.099%KU647676.1
18811881100%0.099%KU870645.1
18811881100%0.099%KU926310.1
18811881100%0.099%KU729217.2
18811881100%0.099%KU820897.1
18811881100%0.099%KU321639.1
18751875100%0.099%KU991811.1
18751875100%0.099%KU926309.1
18751875100%0.099%KU922960.1
18751875100%0.099%KU922923.1
18751875100%0.099%KU853013.1
18751875100%0.099%KU853012.1
18751875100%0.099%KU707826.1
18751875100%0.099%KU527068.1
18751875100%0.099%KU501217.1
18751875100%0.099%KU501216.1
18751875100%0.099%KU501215.1
18751875100%0.099%KU365780.1
18751875100%0.099%KU365779.1
18751875100%0.099%KU365777.1
18691869100%0.099%KU940228.1
18691869100%0.099%KU940224.1
18691869100%0.099%KU820898.1
18691869100%0.099%KU729218.1
18691869100%0.099%KU312312.1
18641864100%0.099%KX056898.1
18641864100%0.099%KU963796.1
18641864100%0.099%KU955590.1
18641864100%0.099%KU955589.1
18641864100%0.099%KU740184.2
18641864100%0.099%KU820899.2
18641864100%0.099%KU761564.1
18641864100%0.099%KU744693.1
18641864100%0.099%KU365778.1
18581858100%0.099%KF993678.1
18421842100%0.099%KU866423.1
18421842100%0.099%KU681081.3
18031803100%0.098%KU955593.1
1803180395%0.0100%KM078936.1
18031803100%0.098%JN860885.1
18031803100%0.098%EU545988.1
1799179995%0.099%KM078961.1
1797179795%0.099%KM078930.1
1794179495%0.099%KM078971.1
1794179495%0.099%KM078970.1
1794179495%0.099%KM078933.1
1792179295%0.099%KM078929.1
1786178696%0.099%KU179098.1
17751775100%0.098%KU681082.3
1650165087%0.0100%KJ873160.1
16091609100%0.095%HQ234499.1
1463146377%0.0100%KJ873161.1
1419141977%0.099%KM851039.1
1384138473%0.099%KU985087.1
1378137873%0.099%KU556802.1
1375137577%0.098%KM851038.1
1267126767%0.099%KU232300.1
1258125867%0.099%KU232290.1
1254125467%0.099%KU232297.1
1249124966%0.099%KU232294.1
1245124566%0.099%KU232292.1
1242124265%0.099%KU232298.1
1238123865%0.099%KU232296.1
1238123866%0.099%KU232293.1
1234123465%0.099%KU232295.1
1227122799%0.088%KU963573.1
1227122799%0.088%KU955594.1
1227122799%0.088%KU720415.1
1227122799%0.088%LC002520.1
1227122799%0.088%HQ234498.1
1227122799%0.088%AY632535.2
1223122364%0.099%KU232288.1
1223122399%0.088%KF268949.1
1223122399%0.088%AF013415.1
12211221100%0.088%DQ859059.1
1219121964%0.099%KU232289.1
1218121899%0.088%KU955595.1
1218121899%0.088%KU955591.1
1216121664%0.099%KU232299.1
1212121299%0.088%KU955592.1
1210121065%0.099%KU232291.1
1206120699%0.088%KF383116.1
1205120599%0.088%KF383121.1
1205120599%0.088%KF383119.1
1192119299%0.088%KF268950.1
1192119299%0.088%KF268948.1
1190119099%0.088%HQ234501.1
1182118299%0.088%KF383118.1
1179117999%0.087%KU963574.1
1179117999%0.087%HQ234500.1
1173117399%0.087%KF383117.1
1123112399%0.087%KF383115.1
99899852%0.0100%KU867812.1
84384399%0.082%KF383120.1
84184169%0.088%KF383103.1
84184169%0.088%KF383086.1
80880869%0.087%KF383104.1
80280269%0.087%KF383085.1
79779769%0.087%KF383106.1
79179169%0.087%KF383114.1
79179169%0.087%KF383107.1
79179169%0.087%KF383088.1
78578569%0.087%KF383087.1
76976969%0.086%KF383089.1
76376369%0.086%KF383101.1
76376369%0.086%KF383097.1
75875869%0.086%KF383099.1
75275269%0.086%KF383098.1
71371369%0.085%KF383113.1
55555569%7e-15781%KF383092.1
55355369%3e-15681%KF383093.1
54254269%5e-15381%KF383091.1
475121464%6e-133100%KU940227.1
43143123%1e-11998%KF258813.1
Posted
Sequences producing significant alignments:

Select:AllNone Selected:0

Sequences producing significant alignments:
Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccession
18031803100%0.0100%KX059014.1
18031803100%0.0100%KX059013.1
18031803100%0.0100%KX051563.1
18031803100%0.0100%KU509998.3
18031803100%0.0100%KM078936.1
18031803100%0.0100%KJ776791.1
17991799100%0.099%KM078961.1
17971797100%0.099%KU497555.1
17971797100%0.099%KU647676.1
17971797100%0.099%KM078930.1
17941794100%0.099%KM078971.1
17941794100%0.099%KM078970.1
17941794100%0.099%KM078933.1
17921792100%0.099%KU870645.1
17921792100%0.099%KU926310.1
17921792100%0.099%KU729217.2
17921792100%0.099%KU820897.1
17921792100%0.099%KU321639.1
17921792100%0.099%KM078929.1
17861786100%0.099%KU991811.1
17861786100%0.099%KU926309.1
17861786100%0.099%KU922960.1
17861786100%0.099%KU922923.1
17861786100%0.099%KU853013.1
17861786100%0.099%KU853012.1
17861786100%0.099%KU707826.1
17861786100%0.099%KU527068.1
17861786100%0.099%KU501217.1
17861786100%0.099%KU501216.1
17861786100%0.099%KU501215.1
17861786100%0.099%KU365780.1
17861786100%0.099%KU365779.1
17861786100%0.099%KU365777.1
17811781100%0.099%KU940228.1
17811781100%0.099%KU940224.1
17811781100%0.099%KU820898.1
17811781100%0.099%KU729218.1
17811781100%0.099%KU312312.1
17751775100%0.099%KX056898.1
17751775100%0.099%KU963796.1
17751775100%0.099%KU955590.1
17751775100%0.099%KU955589.1
17751775100%0.099%KU740184.2
17751775100%0.099%KU820899.2
17751775100%0.099%KU761564.1
17751775100%0.099%KU744693.1
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