niman

Laboratory-confirmed Zika virus disease cases reported to ArboNET by state or territory — United States, 2015–2016 (as of March 30, 2016) http://www.cdc.gov/zika/geo/united-states.html StatesTravel-associated cases* No. (%) (N=312)Locally acquired cases† No. (%) (N=0)Alabama2 (1)0 (0)Arizona1 (<1)0 (0)Arkansas1 (<1)0 (0)California17 (6)0 (0)Colorado2 (1)0 (0)Connecticut1 (<1)0 (0)Delaware3 (1)0 (0)District of Columbia3 (1)0 (0)Florida74 (24)0 (0)Georgia9 (3)0 (0)Hawaii5 (2)0 (0)Illinois9 (3)0 (0)Indiana5 (2)0 (0)Iowa4 (1)0 (0)Kansas1 (<1)0 (0)Kentucky3 (1)0 (0)Louisiana2 (1)0 (0)Maine1 (<1)0 (0)Maryland6 (2)0 (0)Massachusetts7 (2)0 (0)Michigan2 (1)0 (0)Minnesota12 (4)0 (0)Mississippi2 (1)0 (0)Missouri1 (<1)0 (0)Montana1 (<1)0 (0)Nebraska2 (1)0 (0)Nevada2 (<1)0 (0)New Hampshire2 (1)0 (0)New Jersey5 (1)0 (0)New York46 (15)0 (0)North Carolina7 (2)0 (0)Ohio9 (3)0 (0)Oklahoma3 (1)0 (0)Oregon6 (2)0 (0)Pennsylvania11 (4)0 (0)Tennessee1 (<1)0 (0)Texas27 (9)0 (0)Utah2 (1)0 (0)

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Laboratory-confirmed Zika virus disease cases reported to ArboNET by state or territory — United States, 2015–2016 (as of March 30, 2016) http://www.cdc.gov/zika/geo/united-states.html StatesTravel-associated cases* No. (%) (N=312)Locally acquired cases† No. (%) (N=0)Alabama2 (1)0 (0)Arizona1 (<1)0 (0)Arkansas1 (<1)0 (0)California17 (6)0 (0)Colorado2 (1)0 (0)Connecticut1 (<1)0 (0)Delaware3 (1)0 (0)District of Columbia3 (1)0 (0)Florida74 (24)0 (0)Georgia9 (3)0 (0)Hawaii5 (2)0 (0)Illinois9 (3)0 (0)Indiana5 (2)0 (0)Iowa4 (1)0 (0)Kansas1 (<1)0 (0)Kentucky3 (1)0 (0)Louisiana2 (1)0 (0)Maine1 (<1)0 (0)Maryland6 (2)0 (0)Massachusetts7 (2)0 (0)Michigan2 (1)0 (0)Minnesota12 (4)0 (0)Mississippi2 (1)0 (0)Missouri1 (<1)0 (0)Montana1 (<1)0 (0)Nebraska2 (1)0 (0)Nevada2 (<1)0 (0)New Hampshire2 (1)0 (0)New Jersey5 (1)0 (0)

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Laboratory-confirmed Zika virus disease cases reported to ArboNET by state or territory — United States, 2015–2016 (as of March 30, 2016) http://www.cdc.gov/zika/geo/united-states.html StatesTravel-associated cases* No. (%) (N=312)Locally acquired cases† No. (%) (N=0)Alabama2 (1)0 (0)Arizona1 (<1)0 (0)Arkansas1 (<1)0 (0)California17 (6)0 (0)Colorado2 (1)0 (0)Connecticut1 (<1)0 (0)Delaware3 (1)0 (0)District of Columbia3 (1)0 (0)Florida74 (24)0 (0)Georgia9 (3)0 (0)Hawaii5 (2)0 (0)Illinois9 (3)0 (0)Indiana5 (2)0 (0)Iowa4 (1)0 (0)Kansas1 (<1)0 (0)Kentucky3 (1)0 (0)Louisiana2 (1)0 (0)Maine1 (<1)0 (0)Maryland6 (2)0 (0)Massachusetts7 (2)0 (0)Michigan2 (1)0 (0)Minnesota12 (4)0 (0)

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Laboratory-confirmed Zika virus disease cases reported to ArboNET by state or territory — United States, 2015–2016 (as of March 30, 2016) http://www.cdc.gov/zika/geo/united-states.html StatesTravel-associated cases* No. (%) (N=312)Locally acquired cases† No. (%) (N=0)Alabama2 (1)0 (0)Arizona1 (<1)0 (0)Arkansas1 (<1)0 (0)California17 (6)0 (0)Colorado2 (1)0 (0)Connecticut1 (<1)0 (0)Delaware3 (1)0 (0)District of Columbia3 (1)0 (0)Florida74 (24)0 (0)Georgia9 (3)0 (0)Hawaii5 (2)0 (0)Illinois9 (3)0 (0)Indiana5 (2)0 (0)Iowa4 (1)0 (0)Kansas1 (<1)0 (0)Kentucky3 (1)0 (0)Louisiana2 (1)0 (0)Maine1 (<1)0 (0)Maryland6 (2)0 (0)Massachusetts7 (2)0 (0)

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Maryland Confirmed Zika Virus Infections (As of March 30, 2016) http://phpa.dhmh.maryland.gov/Pages/Zika.aspx Travel-AssociatedLocally Acquired Vector-BorneTotal606

Maryland Confirmed Zika Virus Infections (As of March 30, 2016) Travel-AssociatedLocally Acquired Vector-BorneTotal606

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Laboratory-confirmed Zika virus disease cases reported to ArboNET by state or territory — United States, 2015–2016 (as of March 30, 2016) http://www.cdc.gov/zika/geo/united-states.html StatesTravel-associated cases* No. (%) (N=312)Locally acquired cases† No. (%) (N=0)Alabama2 (1)0 (0)Arizona1 (<1)0 (0)Arkansas1 (<1)0 (0)California17 (6)0 (0)Colorado2 (1)0 (0)Connecticut1 (<1)0 (0)Delaware3 (1)0 (0)District of Columbia3 (1)0 (0)Florida74 (24)0 (0)Georgia9 (3)0 (0)Hawaii5 (2)0 (0)Illinois9 (3)0 (0)Indiana5 (2)0 (0)

Zika Virus – March 31, 2016. Texas has had 27 confirmed cases of Zika virus disease. Of those, 26 were in travelers who were infected abroad and diagnosed after they returned home; one of those travelers was a pregnant woman. One case involved a Dallas County resident who had sexual contact with someone who acquired the Zika infection while traveling abroad. Case counts by county: Bexar – 3Dallas – 4Fort Bend – 2Grayson – 1Harris – 11Tarrant – 3Travis – 2Wise – 1

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KU991811.1|Zika virus isolate Brazil/2016/INMI1 polyprotein gene, complete cds650650100%0.0100%KU991811.1Select seq gb|KU985088.1|Zika virus isolate MEX/InDRE/Zika-2/2015 envelope protein gene, partial cds650650100%0.0100%KU985088.1Select seq gb|KU940228.1|Zika virus isolate Bahia07, partial genome650650100%0.0100%KU940228.1Select seq gb|KU940224.1|Zika virus isolate Bahia09, partial genome650650100%0.0100%KU940224.1Select seq gb|KU955589.1|Zika virus isolate Z16006 polyprotein gene, complete cds650650100%0.0100%KU955589.1Select seq gb|KU870645.1|Zika virus isolate FB-GWUH-2016, complete genome650650100%0.0100%KU870645.1Select seq gb|KU926309.1|Zika virus isolate Rio-U1, complete genome650650100%0.0100%KU926309.1Select seq gb|KU866423.1|Zika virus isolate Zika virus/SZ01/2016 polyprotein gene, complete cds650650100%0.0100%KU866423.1Select seq gb|KU820899.2|Zika virus isolate ZJ03, complete genome650650100%0.0100%KU820899.2Select seq gb|KU729217.2|Zika virus isolate BeH823339 polyprotein gene, complete cds650650100%0.0100%KU729217.2Select seq gb|KU729218.1|Zika virus isolate BeH828305 polyprotein gene, complete cds650650100%0.0100%KU729218.1Select seq gb|KU681081.3|Zika virus isolate Zika virus/H.sapiens-tc/THA/2014/SV0127- 14, complete genome650650100%0.0100%KU681081.3Select seq gb|KU527068.1|Zika virus strain Natal RGN, complete genome650650100%0.0100%KU527068.1Select seq gb|KU501217.1|Zika virus strain 8375 polyprotein gene, complete cds650650100%0.0100%KU501217.1Select seq gb|KU501216.1|Zika virus strain 103344 polyprotein gene, complete cds650650100%0.0100%KU501216.1Select seq gb|KJ634273.1|Zika virus strain CK-ISL 2014 E protein (E) gene, partial cds650650100%0.0100%KJ634273.1Select seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds650650100%0.0100%KJ776791.1Select seq gb|KU955590.1|Zika virus isolate Z16019 polyprotein gene, complete cds645645100%0.099%KU955590.1Select seq gb|KU926310.1|Zika virus isolate Rio-S1, complete genome645645100%0.099%KU926310.1Select seq gb|KU922960.1|Zika virus isolate MEX/InDRE/Sm/2016, complete genome645645100%0.099%KU922960.1Select seq gb|KU922923.1|Zika virus isolate MEX/InDRE/Lm/2016, complete genome645645100%0.099%KU922923.1Select seq gb|KU853013.1|Zika virus isolate Dominican Republic/2016/PD2, complete genome645645100%0.099%KU853013.1Select seq gb|KU853012.1|Zika virus isolate Dominican Republic/2016/PD1, complete genome645645100%0.099%KU853012.1Select seq gb|KU497555.1|Zika virus isolate Brazil-ZKV2015, complete genome645645100%0.099%KU497555.1Select seq gb|KU646828.1|Zika virus isolate Si322 polyprotein gene, partial cds645645100%0.099%KU646828.1Select seq gb|KU646827.1|Zika virus isolate Si323 polyprotein gene, partial cds645645100%0.099%KU646827.1Select seq gb|KU647676.1|Zika virus strain MRS_OPY_Martinique_PaRi_2015 polyprotein gene, complete cds645645100%0.099%KU647676.1Select seq gb|KU509998.1|Zika virus strain Haiti/1225/2014, complete genome645645100%0.099%KU509998.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds645645100%0.099%KU365778.1Select seq gb|KU312315.1|Zika virus isolate Z1106027 polyprotein gene, partial cds645645100%0.099%KU312315.1Select seq gb|KU312314.1|Zika virus isolate Z1106031 polyprotein gene, partial cds645645100%0.099%KU312314.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome645645100%0.099%KU321639.1Select seq gb|KU820898.1|Zika virus isolate GZ01 polyprotein gene, complete cds641641100%3e-18099%KU820898.1Select seq gb|KU740184.2|Zika virus isolate GD01 polyprotein gene, complete cds641641100%3e-18099%KU740184.2Select seq gb|KU820897.1|Zika virus isolate FLR polyprotein gene, complete cds641641100%3e-18099%KU820897.1Select seq gb|KU761564.1|Zika virus isolate GDZ16001 polyprotein gene, complete cds641641100%3e-18099%KU761564.1Select seq gb|KU707826.1|Zika virus isolate SSABR1, complete genome641641100%3e-18099%KU707826.1Select seq gb|KU501215.1|Zika virus strain PRVABC59, complete genome641641100%3e-18099%KU501215.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds641641100%3e-18099%KU365780.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds641641100%3e-18099%KU365779.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds641641100%3e-18099%KU365777.1Select seq gb|KU312313.1|Zika virus isolate Z1106032 polyprotein gene, partial cds641641100%3e-18099%KU312313.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds641641100%3e-18099%KU312312.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds641641100%3e-18099%EU545988.1Select seq gb|KU955593.1|Zika virus isolate Zika virus/H.sapiens-tc/KHM/2010/FSS13025, complete genome636636100%1e-17899%KU955593.1Select seq gb|KU681082.3|Zika virus isolate Zika virus/H.sapiens-tc/PHL/2012/CPC-0740, complete genome636636100%1e-17899%KU681082.3Select seq gb|KU744693.1|Zika virus isolate VE_Ganxian, complete genome636636100%1e-17899%KU744693.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds636636100%1e-17899%JN860885.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds632632100%1e-17799%KF993678.1

LOCUS KU985088 360 bp RNA linear VRL 28-MAR-2016 DEFINITION Zika virus isolate MEX/InDRE/Zika-2/2015 envelope protein gene, partial cds. ACCESSION KU985088 VERSION KU985088.1 GI:1011619266 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus. REFERENCE 1 (bases 1 to 360) AUTHORS Diaz-Quinonez,J.A., Vazquez-Pichardo,M., Nunez-Leon,A., Torres-Rodriguez,M.D.L.L., Escobar-Escamilla,N., Wong-Arambula,C.E., Torres-Longoria,B., Lopez-Martinez,I., Ruiz-Matus,C., Kuri-Morales,P.A. and Ramirez-Gonzalez,J.E. TITLE Molecular characterization of Zika virus in Mexico JOURNAL Unpublished REFERENCE 2 (bases 1 to 360) AUTHORS Vazquez-Pichardo,M., Nunez-Leon,A., Torres-Rodriguez,M.L., Rosales-Jimenez,C., Torres-Olmos,Y.A., Perez-Meza,R., Aparicio-Antonio,A.M., Herrera-Bautista,R.M., Wong-Arambula,C.E., Rodriguez-Maldonado,A.P., Del Mazo-Lopez,J.C., Serrano-Gutierrez,S., Escobar-Escamilla,N., Torres-Longoria,B., Gomez-Bustamante,A., Lopez-Martinez,I., Ramirez-Gonzalez,J.E. and Diaz-Quinonez,J.A. TITLE Direct Submission JOURNAL Submitted (28-MAR-2016) Biologia Molecular y Validacion de Tecnicas, Instituto de Diagnostico y Referencia Epidemiologicos (InDRE), Francisco de P. Miranda 177 Col. Lomas de Plateros, Mexico, DF 01480, Mexico COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..360 /organism="Zika virus" /mol_type="genomic RNA" /isolate="MEX/InDRE/Zika-2/2015" /isolation_source="Serum" /host="Homo sapiens; 46 years old; male" /db_xref="taxon:64320" /country="Mexico: Chiapas" /collection_date="30-Nov-2015" CDS <1..>360 /codon_start=2 /product="envelope protein" /protein_id="AMR96779.1" /db_xref="GI:1011619267" /translation="RCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTC AKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSP RAEATLGGFGSLGLDCEP" ORIGIN 1 ccgctgccca acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtctg 61 caaaagaacg ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag 121 cctggtgaca tgcgctaagt ttgcatgctc caagaaaatg accgggaaga gcatccagcc 181 agagaatctg gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgat 241 cgttaatgac acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa 301 ttcaccaaga gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KU985087.1|Zika virus isolate MEX/InDRE/Zika-2/2015 nonstructural protein 5 gene, partial cds13621362100%0.0100%KU985087.1Select seq gb|KU870645.1|Zika virus isolate FB-GWUH-2016, complete genome13621362100%0.0100%KU870645.1Select seq gb|KU497555.1|Zika virus isolate Brazil-ZKV2015, complete genome13571357100%0.099%KU497555.1Select seq gb|KU647676.1|Zika virus strain MRS_OPY_Martinique_PaRi_2015 polyprotein gene, complete cds13571357100%0.099%KU647676.1Select seq gb|KU501217.1|Zika virus strain 8375 polyprotein gene, complete cds13571357100%0.099%KU501217.1Select seq gb|KU501216.1|Zika virus strain 103344 polyprotein gene, complete cds13571357100%0.099%KU501216.1Select seq gb|KU991811.1|Zika virus isolate Brazil/2016/INMI1 polyprotein gene, complete cds13531353100%0.099%KU991811.1Select seq gb|KU926310.1|Zika virus isolate Rio-S1, complete genome13531353100%0.099%KU926310.1Select seq gb|KU926309.1|Zika virus isolate Rio-U1, complete genome13531353100%0.099%KU926309.1Select seq gb|KU729217.2|Zika virus isolate BeH823339 polyprotein gene, complete cds13531353100%0.099%KU729217.2Select seq gb|KU820897.1|Zika virus isolate FLR polyprotein gene, complete cds13531353100%0.099%KU820897.1Select seq gb|KU509998.1|Zika virus strain Haiti/1225/2014, complete genome13531353100%0.099%KU509998.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome13531353100%0.099%KU321639.1Select seq gb|KM078936.1|Zika virus strain CHI1410214 NS5 protein gene, partial cds13531353100%0.099%KM078936.1Select seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds13531353100%0.099%KJ776791.1Select seq gb|KM078961.1|Zika virus strain CHI2612114 NS5 protein gene, partial cds13501350100%0.099%KM078961.1Select seq gb|KU940228.1|Zika virus isolate Bahia07, partial genome13481348100%0.099%KU940228.1Select seq gb|KU940224.1|Zika virus isolate Bahia09, partial genome13481348100%0.099%KU940224.1Select seq gb|KU922960.1|Zika virus isolate MEX/InDRE/Sm/2016, complete genome13481348100%0.099%KU922960.1Select seq gb|KU922923.1|Zika virus isolate MEX/InDRE/Lm/2016, complete genome13481348100%0.099%KU922923.1Select seq gb|KU853013.1|Zika virus isolate Dominican Republic/2016/PD2, complete genome13481348100%0.099%KU853013.1Select seq gb|KU853012.1|Zika virus isolate Dominican Republic/2016/PD1, complete genome13481348100%0.099%KU853012.1Select seq gb|KU707826.1|Zika virus isolate SSABR1, complete genome13481348100%0.099%KU707826.1Select seq gb|KU527068.1|Zika virus strain Natal RGN, complete genome13481348100%0.099%KU527068.1Select seq gb|KU501215.1|Zika virus strain PRVABC59, complete genome13481348100%0.099%KU501215.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds13481348100%0.099%KU365780.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds13481348100%0.099%KU365779.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds13481348100%0.099%KU365777.1Select seq gb|KM078930.1|Zika virus strain CHI2283714 NS5 protein gene, partial cds13481348100%0.099%KM078930.1Select seq gb|KM078971.1|Zika virus strain CHI2613014 NS5 protein gene, partial cds13461346100%0.099%KM078971.1Select seq gb|KM078970.1|Zika virus strain CHI2490414 NS5 protein gene, partial cds13461346100%0.099%KM078970.1Select seq gb|KM078933.1|Zika virus strain CHI1058514 NS5 protein gene, partial cds13461346100%0.099%KM078933.1Select seq gb|KU955590.1|Zika virus isolate Z16019 polyprotein gene, complete cds13441344100%0.099%KU955590.1Select seq gb|KU820898.1|Zika virus isolate GZ01 polyprotein gene, complete cds13441344100%0.099%KU820898.1Select seq gb|KU740184.2|Zika virus isolate GD01 polyprotein gene, complete cds13441344100%0.099%KU740184.2Select seq gb|KU729218.1|Zika virus isolate BeH828305 polyprotein gene, complete cds13441344100%0.099%KU729218.1Select seq gb|KU761564.1|Zika virus isolate GDZ16001 polyprotein gene, complete cds13441344100%0.099%KU761564.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds13441344100%0.099%KU365778.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds13441344100%0.099%KU312312.1Select seq gb|KM078929.1|Zika virus strain CHI1805214 NS5 protein gene, partial cds13441344100%0.099%KM078929.1Select seq gb|KU556802.1|Zika virus isolate MEX/InDRE/14/2015 NS5 protein gene, partial cds1341134198%0.099%KU556802.1Select seq gb|KU955589.1|Zika virus isolate Z16006 polyprotein gene, complete cds13301330100%0.099%KU955589.1Select seq gb|KU866423.1|Zika virus isolate Zika virus/SZ01/2016 polyprotein gene, complete cds13301330100%0.099%KU866423.1Select seq gb|KU820899.2|Zika virus isolate ZJ03, complete genome13301330100%0.099%KU820899.2Select seq gb|KU744693.1|Zika virus isolate VE_Ganxian, complete genome13301330100%0.099%KU744693.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds13301330100%0.099%KF993678.1Select seq gb|KJ873160.1|Zika virus isolate NC14-03042014-3481 nonstructural protein 5 gene, partial cds1328132898%0.099%KJ873160.1Select seq gb|KU681081.3|Zika virus isolate Zika virus/H.sapiens-tc/THA/2014/SV0127- 14, complete genome13261326100%0.099%KU681081.3Select seq gb|KU179098.1|Zika virus isolate JMB-185 nonstructural protein 5 gene, partial cds13261326100%0.099%KU179098.1Select seq gb|KU955593.1|Zika virus isolate Zika virus/H.sapiens-tc/KHM/2010/FSS13025, complete genome13031303100%0.098%KU955593.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds13031303100%0.098%JN860885.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds12991299100%0.098%EU545988.1Select seq gb|KU681082.3|Zika virus isolate Zika virus/H.sapiens-tc/PHL/2012/CPC-0740, complete genome12811281100%0.098%KU681082.3Select seq gb|KJ873161.1|Zika virus isolate NC14-02042014-3220 nonstructural protein 5 gene, partial cds1249124992%0.099%KJ873161.1Select seq gb|KU232300.1|Zika virus isolate 067ZV_PEBR15 NS5 protein gene, partial cds1240124091%0.099%KU232300.1Select seq gb|KU232290.1|Zika virus isolate 036ZV_PEBR15 NS5 protein gene, partial cds1231123190%0.099%KU232290.1Select seq gb|KU232297.1|Zika virus isolate 049ZV_PEBR15 NS5 protein gene, partial cds1229122991%0.099%KU232297.1Select seq gb|KU232294.1|Zika virus isolate 061ZV_PEBR15 NS5 protein gene, partial cds1220122089%0.099%KU232294.1Select seq gb|KU232292.1|Zika virus isolate 054ZV_PEBR15 NS5 protein gene, partial cds1218121890%0.099%KU232292.1Select seq gb|KU232298.1|Zika virus isolate 050ZV_PEBR15 NS5 protein gene, partial cds1214121489%0.099%KU232298.1Select seq gb|KU232296.1|Zika virus isolate 045ZV_PEBR15 NS5 protein gene, partial cds1211121189%0.099%KU232296.1Select seq gb|KU232293.1|Zika virus isolate 057ZV_PEBR15 NS5 protein gene, partial cds1211121189%0.099%KU232293.1Select seq gb|KU232295.1|Zika virus isolate 068ZV_PEBR15 NS5 protein gene, partial cds1205120588%0.099%KU232295.1Select seq gb|KU232288.1|Zika virus isolate 001ZV_PEBR15 NS5 protein gene, partial cds1195119588%0.099%KU232288.1Select seq gb|KU232289.1|Zika virus isolate 020ZV_PEBR15 NS5 protein gene, partial cds1191119187%0.099%KU232289.1Select seq gb|KU232299.1|Zika virus isolate 015ZV_PEBR15 NS5 protein gene, partial cds1187118787%0.099%KU232299.1Select seq gb|KU232291.1|Zika virus isolate 051ZV_PEBR15 NS5 protein gene, partial cds1186118688%0.099%KU232291.1Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds11641164100%0.094%HQ234499.1Select seq gb|KM851039.1|Zika virus strain SV0127/14 nonstructural protein 5 gene, partial cds1047104778%0.099%KM851039.1Select seq gb|KM851038.1|Zika virus strain CPC-0740 nonstructural protein 5 gene, partial cds1016101678%0.098%KM851038.1Select seq gb|KF268949.1|Zika virus isolate ARB15076 polyprotein gene, complete cds969969100%0.088%KF268949.1Select seq gb|KU955594.1|Zika virus isolate Zika virus/M.mulatta-tc/UGA/1947/MR-766, complete genome966966100%0.088%KU955594.1Select seq gb|KU720415.1|Zika virus strain MR 766 polyprotein gene, complete cds966966100%0.088%KU720415.1Select seq dbj|LC002520.1|Zika virus genomic RNA, complete genome, strain: MR766-NIID966966100%0.088%LC002520.1Select seq gb|HQ234498.1|Zika virus isolate MR_766 polyprotein gene, partial cds966966100%0.088%HQ234498.1Select seq gb|AY632535.2|Zika virus strain MR 766, complete genome966966100%0.088%AY632535.2Select seq gb|AF013415.1|Zika virus strain MR-766 NS5 protein (NS5) gene, partial cds966966100%0.088%AF013415.1Select seq gb|DQ859059.1|Zika virus strain MR 766 polyprotein gene, complete cds960960100%0.088%DQ859059.1Select seq gb|KF383121.1|Zika virus strain ArD158095 polyprotein gene, partial cds957957100%0.088%KF383121.1Select seq gb|KF383119.1|Zika virus strain ArD158084 polyprotein gene, complete cds957957100%0.088%KF383119.1Select seq gb|KU955595.1|Zika virus isolate Zika virus/A.taylori-tc/SEN/1984/41671-DAK, complete genome948948100%0.088%KU955595.1Select seq gb|KU955592.1|Zika virus isolate Zika virus/A.taylori-tc/SEN/1984/41662-DAK, complete genome948948100%0.088%KU955592.1Select seq gb|KU955591.1|Zika virus isolate Zika virus/A.africanus-tc/SEN/1984/41525-DAK, complete genome948948100%0.088%KU955591.1Select seq gb|KF268950.1|Zika virus isolate ARB7701 polyprotein gene, complete cds946946100%0.088%KF268950.1Select seq gb|KF268948.1|Zika virus isolate ARB13565 polyprotein gene, complete cds946946100%0.088%KF268948.1Select seq gb|KF383116.1|Zika virus strain ArD7117 polyprotein gene, complete cds939939100%0.088%KF383116.1Select seq gb|KF383118.1|Zika virus strain ArD157995 polyprotein gene, complete cds933933100%0.087%KF383118.1Select seq gb|HQ234501.1|Zika virus isolate ArD_41519 polyprotein gene, partial cds930930100%0.087%HQ234501.1Select seq gb|HQ234500.1|Zika virus isolate IbH_30656 polyprotein gene, partial cds930930100%0.087%HQ234500.1Select seq gb|KF383117.1|Zika virus strain ArD128000 polyprotein gene, complete cds915915100%0.087%KF383117.1Select seq gb|KF383115.1|Zika virus strain ArB1362 polyprotein gene, complete cds901901100%0.087%KF383115.1Select seq gb|KF383103.1|Zika virus strain ArA986 nonstructural protein 5 gene, partial cds88888891%0.088%KF383103.1Select seq gb|KF383086.1|Zika virus strain ArA975 nonstructural protein 5 gene, partial cds88888891%0.088%KF383086.1Select seq gb|KF383104.1|Zika virus strain ArA982 nonstructural protein 5 gene, partial cds85685691%0.087%KF383104.1Select seq gb|KF383085.1|Zika virus strain ArD9957 nonstructural protein 5 gene, partial cds85285291%0.087%KF383085.1Select seq gb|KF383106.1|Zika virus strain ArA27443 nonstructural protein 5 gene, partial cds84784791%0.087%KF383106.1Select seq gb|KF383114.1|Zika virus strain AnD30332 nonstructural protein 5 gene, partial cds84384391%0.087%KF383114.1Select seq gb|KF383107.1|Zika virus strain ArA27407 nonstructural protein 5 gene, partial cds84384391%0.087%KF383107.1Select seq gb|KF383088.1|Zika virus strain ArD30101 nonstructural protein 5 gene, partial cds84384391%0.087%KF383088.1Select seq gb|KF383087.1|Zika virus strain ArD30156 nonstructural protein 5 gene, partial cds83883891%0.087%KF383087.1Select seq gb|KF383089.1|Zika virus strain ArD165531 nonstructural protein 5 gene, partial cds82582591%0.086%KF383089.1Select seq gb|KF383101.1|Zika virus strain ArD127710 nonstructural protein 5 gene, partial cds82082091%0.086%KF383101.1Select seq gb|KF383097.1|Zika virus strain ArD127994 nonstructural protein 5 gene, partial cds82082091%0.086%KF383097.1Select seq gb|KF383099.1|Zika virus strain ArD127987 nonstructural protein 5 gene, partial cds81681691%0.086%KF383099.1Select seq gb|KF383098.1|Zika virus strain ArD127988 nonstructural protein 5 gene, partial cds81081091%0.086%KF383098.1Select seq gb|KF383084.1|Zika virus strain HD78788 nonstructural protein 5 gene, partial cds78978991%0.085%KF383084.1Select seq gb|KF383113.1|Zika virus strain ArA1465 nonstructural protein 5 gene, partial cds78078091%0.085%KF383113.1Select seq gb|KF383120.1|Zika virus strain ArD142623 nonfunctional polyprotein gene, partial sequence762762100%0.082%KF383120.1Select seq gb|KF383092.1|Zika virus strain ArD147917 nonstructural protein 5 gene, partial cds64164191%6e-18081%KF383092.1Select seq gb|KF383093.1|Zika virus strain ArD149810 nonstructural protein 5 gene, partial cds63963991%2e-17980%KF383093.1Select seq gb|KF383095.1|Zika virus strain ArD132915 nonstructural protein 5 gene, partial cds63663691%3e-17880%KF383095.1Select seq gb|KF383091.1|Zika virus strain ArD149938 nonstructural protein 5 gene, partial cds63063091%1e-17680%KF383091.1Select seq gb|KU867812.1|Zika virus isolate Jiangxi.CHN/01/2016 nonstructural protein 5 gene, partial cds61861845%7e-173100%KU867812.1Select seq gb|KU940227.1|Zika virus isolate Bahia08, partial genome33172353%2e-8699%KU940227.1

LOCUS KU985087 755 bp RNA linear VRL 28-MAR-2016 DEFINITION Zika virus isolate MEX/InDRE/Zika-2/2015 nonstructural protein 5 gene, partial cds. ACCESSION KU985087 VERSION KU985087.1 GI:1011619245 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus. REFERENCE 1 (bases 1 to 755) AUTHORS Diaz-Quinonez,J.A., Vazquez-Pichardo,M., Nunez-Leon,A., Torres-Rodriguez,M.D.L.L., Escobar-Escamilla,N., Wong-Arambula,C.E., Torres-Longoria,B., Lopez-Martinez,I., Ruiz-Matus,C., Kuri-Morales,P.A. and Ramirez-Gonzalez,J.E. TITLE Molecular characterization of Zika virus in Mexico JOURNAL Unpublished REFERENCE 2 (bases 1 to 755) AUTHORS Vazquez-Pichardo,M., Nunez-Leon,A., Torres-Rodriguez,M.L., Rosales-Jimenez,C., Torres-Olmos,Y.A., Perez-Meza,R., Aparicio-Antonio,A.M., Herrera-Bautista,R.M., Wong-Arambula,C.E., Rodriguez-Maldonado,A.P., Del Mazo-Lopez,J.C., Serrano-Gutierrez,S., Escobar-Escamilla,N., Torres-Longoria,B., Gomez-Bustamante,A., Lopez-Martinez,I., Ramirez-Gonzalez,J.E. and Diaz-Quinonez,J.A. TITLE Direct Submission JOURNAL Submitted (28-MAR-2016) Biologia Molecular y Validacion de Tecnicas, Instituto de Diagnostico y Referencia Epidemiologicos (InDRE), Francisco de P. Miranda 177 Col. Lomas de Plateros, Mexico, DF 01480, Mexico COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..755 /organism="Zika virus" /mol_type="genomic RNA" /isolate="MEX/InDRE/Zika-2/2015" /isolation_source="Serum" /host="Homo sapiens; 46 years old; male" /db_xref="taxon:64320" /country="Mexico: Chiapas" /collection_date="30-Nov-2015" CDS <1..>755 /codon_start=1 /product="nonstructural protein 5" /protein_id="AMR96778.1" /db_xref="GI:1011619246" /translation="EALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSCIPGGRM YADDTAGWDTRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGK TVMDIISRQDQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTN WLQSNGWDRLKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNW EEVPFCSHHFNKLHLKDGRSIVVPCRHQDELIGR" ORIGIN 1 gaagcccttg gattcttgaa cgaggatcac tggatgggga gagagaactc aggaggtggt 61 gttgaagggc tgggattaca aagactcgga tatgtcctag aagagatgag ttgcatacca 121 ggaggaagga tgtatgcaga tgacactgct ggctgggaca cccgcatcag caggtttgat 181 ctggagaatg aagctctaat caccaaccaa atggagaaag ggcacagggc cttggcattg 241 gccataatca agtacacata ccaaaacaaa gtggtaaagg tccttagacc agctgaaaaa 301 gggaaaacag ttatggacat tatttcgaga caagaccaaa gggggagcgg acaagttgtc 361 acttacgctc ttaacacatt taccaaccta gtggtgcaac tcattcggaa tatggaggct 421 gaggaagttc tagagatgca agacttgtgg ctgctgcgga ggtcagagaa agtgaccaac 481 tggttgcaga gcaacggatg ggataggctc aaacgaatgg cagtcagtgg agatgattgc 541 gttgtgaagc caattgatga taggtttgca catgccctca ggttcttgaa tgatatggga 601 aaagttagga aggacacaca agagtggaaa ccctcaactg gatgggacaa ctgggaagaa 661 gttccgtttt gctcccacca cttcaacaag ctccatctca aggacgggag gtccattgtg 721 gttccctgcc gccaccaaga tgaactgatt ggccg

InDRE released two partial Zika sequences from 46M in Chiapas,Mexico collected Nov 30, 2015 which exactly matches sequence from fetal brain collected from Washington DC traveler (FB-GWUH/2016), infected while on vacation (Nov 2015) that included travel to Mexico and Guatemala.

As of March 30, 2016 (5 am EST) Zika virus disease and Zika virus congenital infection are nationally notifiable conditions.This update from the CDC Arboviral Disease Branch includes provisional data reported to ArboNET for January 1, 2015 – March 30, 2016.US States Travel-associated Zika virus disease cases reported: 312Locally acquired vector-borne cases reported: 0Of the 312 cases reported, 27 were pregnant women, 6 were sexually transmitted, and 1 had Guillain-Barré syndromeUS Territories Travel-associated cases reported: 3Locally acquired cases reported: 349Of the 352 cases reported, 37 were pregnant women and 1 had Guillain-Barré syndrome Laboratory-confirmed Zika virus disease cases reported to ArboNET by state or territory — United States, 2015–2016 (as of March 30, 2016) StatesTravel-associated cases* No. (%) (N=312)Locally acquired cases† No. (%) (N=0)Alabama2 (1)0 (0)Arizona1 (<1)0 (0)Arkansas1 (<1)0 (0)California17 (6)0 (0)Colorado2 (1)0 (0)Connecticut1 (<1)0 (0)Delaware3 (1)0 (0)District of Columbia3 (1)0 (0)Florida74 (24)0 (0)Georgia9 (3)0 (0)Hawaii5 (2)0 (0)Illinois9 (3)0 (0)Indiana5 (2)0 (0)Iowa4 (1)0 (0)Kansas1 (<1)0 (0)Kentucky3 (1)0 (0)Louisiana2 (1)0 (0)Maine1 (<1)0 (0)Maryland6 (2)0 (0)Massachusetts7 (2)0 (0)Michigan2 (1)0 (0)Minnesota12 (4)0 (0)Mississippi2 (1)0 (0)Missouri1 (<1)0 (0)Montana1 (<1)0 (0)Nebraska2 (1)0 (0)Nevada2 (<1)0 (0)New Hampshire2 (1)0 (0)New Jersey5 (1)0 (0)New York46 (15)0 (0)North Carolina7 (2)0 (0)Ohio9 (3)0 (0)Oklahoma3 (1)0 (0)Oregon6 (2)0 (0)Pennsylvania11 (4)0 (0)Tennessee1 (<1)0 (0)Texas27 (9)0 (0)Utah2 (1)0 (0)Virginia8 (3)0 (0)Washington2 (1)0 (0)West Virginia5 (2)0 (0) Territories(N=3)(N=349)American Samoa0 (0)14 (4)Puerto Rico2 (67)325 (93)US Virgin Islands1 (33)10 (3)*Travelers returning from affected areas, their sexual contacts, or infants infected in utero †Presumed local mosquito-borne transmission Page last reviewed: February 4, 2016Page last updated: March 30, 2016

DescriptionsSequences producing significant alignments:Select:AllNone Selected:0AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KU991811.1|Zika virus isolate Brazil/2016/INMI1 polyprotein gene, complete cds1852518525100%0.0100%KU991811.1Select seq gb|KU509998.1|Zika virus strain Haiti/1225/2014, complete genome1842918429100%0.099%KU509998.1Select seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds1842618426100%0.099%KJ776791.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome1841718417100%0.099%KU321639.1Select seq gb|KU729218.1|Zika virus isolate BeH828305 polyprotein gene, complete cds1840818408100%0.099%KU729218.1Select seq gb|KU497555.1|Zika virus isolate Brazil-ZKV2015, complete genome184081840899%0.099%KU497555.1Select seq gb|KU707826.1|Zika virus isolate SSABR1, complete genome1840818408100%0.099%KU707826.1Select seq gb|KU647676.1|Zika virus strain MRS_OPY_Martinique_PaRi_2015 polyprotein gene, complete cds1840818408100%0.099%KU647676.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds1840818408100%0.099%KU365779.1Select seq gb|KU926309.1|Zika virus isolate Rio-U1, complete genome1839318393100%0.099%KU926309.1Select seq gb|KU820897.1|Zika virus isolate FLR polyprotein gene, complete cds1839318393100%0.099%KU820897.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds1839318393100%0.099%KU365780.1Select seq gb|KU940228.1|Zika virus isolate Bahia07, partial genome1839018390100%0.099%KU940228.1Select seq gb|KU922960.1|Zika virus isolate MEX/InDRE/Sm/2016, complete genome1839018390100%0.099%KU922960.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds1839018390100%0.099%KU365777.1Select seq gb|KU922923.1|Zika virus isolate MEX/InDRE/Lm/2016, complete genome1838418384100%0.099%KU922923.1Select seq gb|KU501217.1|Zika virus strain 8375 polyprotein gene, complete cds1838418384100%0.099%KU501217.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds1838418384100%0.099%KU365778.1Select seq gb|KU729217.2|Zika virus isolate BeH823339 polyprotein gene, complete cds1838118381100%0.099%KU729217.2Select seq gb|KU527068.1|Zika virus strain Natal RGN, complete genome1838118381100%0.099%KU527068.1Select seq gb|KU501216.1|Zika virus strain 103344 polyprotein gene, complete cds1838118381100%0.099%KU501216.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds1837518375100%0.099%KU312312.1Select seq gb|KU926310.1|Zika virus isolate Rio-S1, complete genome1836618366100%0.099%KU926310.1Select seq gb|KU853013.1|Zika virus isolate Dominican Republic/2016/PD2, complete genome1836618366100%0.099%KU853013.1Select seq gb|KU853012.1|Zika virus isolate Dominican Republic/2016/PD1, complete genome1836618366100%0.099%KU853012.1Select seq gb|KU501215.1|Zika virus strain PRVABC59, complete genome1836618366100%0.099%KU501215.1Select seq gb|KU870645.1|Zika virus isolate FB-GWUH-2016, complete genome1836318363100%0.099%KU870645.1Select seq gb|KU820898.1|Zika virus isolate GZ01 polyprotein gene, complete cds1835718357100%0.099%KU820898.1Select seq gb|KU740184.2|Zika virus isolate GD01 polyprotein gene, complete cds1834818348100%0.099%KU740184.2Select seq gb|KU761564.1|Zika virus isolate GDZ16001 polyprotein gene, complete cds1834818348100%0.099%KU761564.1Select seq gb|KU955590.1|Zika virus isolate Z16019 polyprotein gene, complete cds1834518345100%0.099%KU955590.1Select seq gb|KU955589.1|Zika virus isolate Z16006 polyprotein gene, complete cds1832118321100%0.099%KU955589.1Select seq gb|KU820899.2|Zika virus isolate ZJ03, complete genome1832118321100%0.099%KU820899.2Select seq gb|KU940224.1|Zika virus isolate Bahia09, partial genome182941829499%0.099%KU940224.1Select seq gb|KU866423.1|Zika virus isolate Zika virus/SZ01/2016 polyprotein gene, complete cds1821318213100%0.099%KU866423.1Select seq gb|KU744693.1|Zika virus isolate VE_Ganxian, complete genome1819118191100%0.099%KU744693.1Select seq gb|KU681081.3|Zika virus isolate Zika virus/H.sapiens-tc/THA/2014/SV0127- 14, complete genome1809218092100%0.099%KU681081.3Select seq gb|KU955593.1|Zika virus isolate Zika virus/H.sapiens-tc/KHM/2010/FSS13025, complete genome1778617786100%0.098%KU955593.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds177841778499%0.098%JN860885.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds177391773998%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds1764017640100%0.098%EU545988.1Select seq gb|KU681082.3|Zika virus isolate Zika virus/H.sapiens-tc/PHL/2012/CPC-0740, complete genome1748317483100%0.098%KU681082.3Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds164751647599%0.096%HQ234499.1Select seq gb|KF383115.1|Zika virus strain ArB1362 polyprotein gene, complete cds1331113311100%0.089%KF383115.1Select seq gb|KF268949.1|Zika virus isolate ARB15076 polyprotein gene, complete cds1330613306100%0.089%KF268949.1Select seq gb|KU720415.1|Zika virus strain MR 766 polyprotein gene, complete cds1330413304100%0.089%KU720415.1Select seq gb|KU955595.1|Zika virus isolate Zika virus/A.taylori-tc/SEN/1984/41671-DAK, complete genome1329913299100%0.089%KU955595.1Select seq gb|HQ234498.1|Zika virus isolate MR_766 polyprotein gene, partial cds132991329999%0.089%HQ234498.1Select seq gb|KF268948.1|Zika virus isolate ARB13565 polyprotein gene, complete cds1329713297100%0.089%KF268948.1Select seq gb|KU955592.1|Zika virus isolate Zika virus/A.taylori-tc/SEN/1984/41662-DAK, complete genome1329013290100%0.089%KU955592.1Select seq gb|KF268950.1|Zika virus isolate ARB7701 polyprotein gene, complete cds1329013290100%0.089%KF268950.1Select seq gb|KF383119.1|Zika virus strain ArD158084 polyprotein gene, complete cds1328613286100%0.089%KF383119.1Select seq dbj|LC002520.1|Zika virus genomic RNA, complete genome, strain: MR766-NIID1328113281100%0.089%LC002520.1Select seq gb|DQ859059.1|Zika virus strain MR 766 polyprotein gene, complete cds1327513275100%0.089%DQ859059.1Select seq gb|KU955591.1|Zika virus isolate Zika virus/A.africanus-tc/SEN/1984/41525-DAK, complete genome1327213272100%0.089%KU955591.1Select seq gb|KU955594.1|Zika virus isolate Zika virus/M.mulatta-tc/UGA/1947/MR-766, complete genome1326513265100%0.089%KU955594.1Select seq gb|KF383116.1|Zika virus strain ArD7117 polyprotein gene, complete cds1326113261100%0.089%KF383116.1Select seq gb|HQ234501.1|Zika virus isolate ArD_41519 polyprotein gene, partial cds132451324599%0.089%HQ234501.1Select seq gb|AY632535.2|Zika virus strain MR 766, complete genome1322313223100%0.089%AY632535.2Select seq gb|KF383117.1|Zika virus strain ArD128000 polyprotein gene, complete cds1318213182100%0.088%KF383117.1Select seq gb|HQ234500.1|Zika virus isolate IbH_30656 polyprotein gene, partial cds131741317499%0.088%HQ234500.1Select seq gb|KF383118.1|Zika virus strain ArD157995 polyprotein gene, complete cds1296313031100%0.088%KF383118.1Select seq gb|KF383121.1|Zika virus strain ArD158095 polyprotein gene, partial cds128821288297%0.089%KF383121.1Select seq gb|KF383120.1|Zika virus strain ArD142623 nonfunctional polyprotein gene, partial sequence108531085397%0.084%KF383120.1Select seq gb|KU940227.1|Zika virus isolate Bahia08, partial genome50381445587%0.095%KU940227.1Select seq gb|KU312314.1|Zika virus isolate Z1106031 polyprotein gene, partial cds4980498027%0.099%KU312314.1Select seq gb|KU312313.1|Zika virus isolate Z1106032 polyprotein gene, partial cds4953495327%0.099%KU312313.1Select seq gb|KU646828.1|Zika virus isolate Si322 polyprotein gene, partial cds4659465925%0.099%KU646828.1Select seq gb|KU646827.1|Zika virus isolate Si323 polyprotein gene, partial cds4650465025%0.099%KU646827.1Select seq gb|KU312315.1|Zika virus isolate Z1106027 polyprotein gene, partial cds3434343418%0.099%KU312315.1Select seq gb|KU740199.1|Zika virus isolate VE_Ganxian2016 polyprotein gene, partial cds3214321417%0.099%KU740199.1Select seq gb|DQ859064.1|Spondweni virus strain SM-6 V-1 polyprotein gene, complete cds2856416495%0.071%DQ859064.1Select seq gb|KJ634273.1|Zika virus strain CK-ISL 2014 E protein (E) gene, partial cds2700270014%0.099%KJ634273.1Select seq gb|KU686218.1|Zika virus isolate MEX/InDRE/14/2015 polyprotein gene, partial cds2057205711%0.099%KU686218.1Select seq gb|KU179098.1|Zika virus isolate JMB-185 nonstructural protein 5 gene, partial cds2017201711%0.099%KU179098.1Select seq gb|KM078936.1|Zika virus strain CHI1410214 NS5 protein gene, partial cds174617469%0.099%KM078936.1Select seq gb|KM078961.1|Zika virus strain CHI2612114 NS5 protein gene, partial cds174517459%0.099%KM078961.1Select seq gb|KM078930.1|Zika virus strain CHI2283714 NS5 protein gene, partial cds174317439%0.099%KM078930.1Select seq gb|KM078971.1|Zika virus strain CHI2613014 NS5 protein gene, partial cds173917399%0.099%KM078971.1Select seq gb|KM078970.1|Zika virus strain CHI2490414 NS5 protein gene, partial cds173917399%0.099%KM078970.1Select seq gb|KM078933.1|Zika virus strain CHI1058514 NS5 protein gene, partial cds173917399%0.099%KM078933.1Select seq gb|KM078929.1|Zika virus strain CHI1805214 NS5 protein gene, partial cds173717379%0.099%KM078929.1Select seq gb|KJ873160.1|Zika virus isolate NC14-03042014-3481 nonstructural protein 5 gene, partial cds159715978%0.099%KJ873160.1Select seq gb|KJ873161.1|Zika virus isolate NC14-02042014-3220 nonstructural protein 5 gene, partial cds141514157%0.099%KJ873161.1Select seq gb|KM851039.1|Zika virus strain SV0127/14 nonstructural protein 5 gene, partial cds138213827%0.099%KM851039.1Select seq gb|KU985087.1|Zika virus isolate MEX/InDRE/Zika-2/2015 nonstructural protein 5 gene, partial cds135313537%0.099%KU985087.1Select seq gb|KU556802.1|Zika virus isolate MEX/InDRE/14/2015 NS5 protein gene, partial cds134613467%0.099%KU556802.1Select seq gb|KM851038.1|Zika virus strain CPC-0740 nonstructural protein 5 gene, partial cds134613467%0.098%KM851038.1Select seq gb|AF013415.1|Zika virus strain MR-766 NS5 protein (NS5) gene, partial cds1310131010%0.088%AF013415.1Select seq gb|KU232300.1|Zika virus isolate 067ZV_PEBR15 NS5 protein gene, partial cds124012406%0.099%KU232300.1Select seq gb|KT200609.1|Zika virus isolate BR/949/15 NS5 gene, partial cds124012406%0.099%KT200609.1Select seq gb|KU232290.1|Zika virus isolate 036ZV_PEBR15 NS5 protein gene, partial cds123112316%0.099%KU232290.1Select seq gb|KU232297.1|Zika virus isolate 049ZV_PEBR15 NS5 protein gene, partial cds122912296%0.099%KU232297.1Select seq gb|KU232294.1|Zika virus isolate 061ZV_PEBR15 NS5 protein gene, partial cds122012206%0.099%KU232294.1Select seq gb|KU232292.1|Zika virus isolate 054ZV_PEBR15 NS5 protein gene, partial cds121812186%0.099%KU232292.1Select seq gb|KU232298.1|Zika virus isolate 050ZV_PEBR15 NS5 protein gene, partial cds121412146%0.099%KU232298.1Select seq gb|KU232296.1|Zika virus isolate 045ZV_PEBR15 NS5 protein gene, partial cds121112116%0.099%KU232296.1Select seq gb|KU232293.1|Zika virus isolate 057ZV_PEBR15 NS5 protein gene, partial cds121112116%0.099%KU232293.1Select seq gb|KU232295.1|Zika virus isolate 068ZV_PEBR15 NS5 protein gene, partial cds120512056%0.099%KU232295.1Select seq gb|KU232288.1|Zika virus isolate 001ZV_PEBR15 NS5 protein gene, partial cds119511956%0.099%KU232288.1Select seq gb|KU232289.1|Zika virus isolate 020ZV_PEBR15 NS5 protein gene, partial cds119111916%0.099%KU232289.1Select seq gb|KU232299.1|Zika virus isolate 015ZV_PEBR15 NS5 protein gene, partial cds118711876%0.099%KU232299.1Select seq gb|KU232291.1|Zika virus isolate 051ZV_PEBR15 NS5 protein gene, partial cds118611866%0.099%KU232291.1Select seq gb|KU758878.1|Zika virus polyprotein gene, partial cds113911396%0.099%KU758878.1Select seq gb|KF270886.1|Zika virus strain CCB-870 envelope glycoprotein gene, partial cds107710778%0.089%KF270886.1Select seq gb|AF372422.1|AF372422Zika virus envelope protein (E) gene, partial cds102010208%0.087%AF372422.1Select seq gb|KU867812.1|Zika virus isolate Jiangxi.CHN/01/2016 nonstructural protein 5 gene, partial cds101210125%0.099%KU867812.1Select seq gb|KF270887.1|Zika virus strain CCB-870 NS3 protein gene, partial cds9969967%0.089%KF270887.1Select seq gb|KF383022.1|Zika virus strain ArD127988 envelope protein gene, partial cds9809807%0.089%KF383022.1Select seq gb|EF158064.1|St. Louis encephalitis virus strain GML 902612 polyprotein gene, partial cds980193161%0.070%EF158064.1Select seq gb|KF383026.1|Zika virus strain ArD127994 envelope protein gene, partial cds9759757%0.089%KF383026.1Select seq gb|KF383032.1|Zika virus strain ArD30101 envelope protein gene, partial cds9669667%0.088%KF383032.1Select seq gb|KF383034.1|Zika virus strain ArD30156 envelope protein gene, partial cds9629627%0.088%KF383034.1Select seq gb|KF383029.1|Zika virus strain ArD165522 envelope protein gene, partial cds9629627%0.088%KF383029.1Select seq gb|EU303241.1|Zika virus note MR766 (p4 15/9/76) nonstructural protein 5 (NS5) gene, partial cds9629627%0.088%EU303241.1Select seq gb|KF383037.1|Zika virus strain ArA506 envelope protein gene, partial cds9609607%0.088%KF383037.1Select seq gb|KF383036.1|Zika virus strain ArA975 envelope protein gene, partial cds9579577%0.088%KF383036.1Select seq gb|KF383033.1|Zika virus strain AnD30332 envelope protein gene, partial cds9579577%0.088%KF383033.1Select seq gb|KF383039.1|Zika virus strain HD78788 envelope protein gene, partial cds9399397%0.088%KF383039.1Select seq gb|KF383028.1|Zika virus strain ArD165531 envelope protein gene, partial cds9399397%0.088%KF383028.1Select seq gb|KF383031.1|Zika virus strain ArD9957 envelope protein gene, partial cds9359357%0.088%KF383031.1Select seq gb|KF383038.1|Zika virus strain ArA986 envelope protein gene, partial cds9309307%0.087%KF383038.1Select seq gb|KF383046.1|Zika virus strain ArA982 envelope protein gene, partial cds9219217%0.087%KF383046.1Select seq gb|EU566860.1|St. Louis encephalitis virus strain Hubbard, complete genome915199857%0.069%EU566860.1Select seq gb|KT823415.1|St. Louis encephalitis virus strain RT 121B polyprotein gene, complete cds913189465%0.069%KT823415.1Select seq gb|FJ753286.2|St. Louis encephalitis virus strain CbaAr-4005, complete genome913191165%0.069%FJ753286.2Select seq gb|EF158062.1|St. Louis encephalitis virus strain FL 79-411 polyprotein gene, partial cds913198059%0.069%EF158062.1Select seq gb|EF158058.1|St. Louis encephalitis virus strain Kern 217 polyprotein gene, partial cds913195559%0.069%EF158058.1Select seq gb|DQ525916.1|St. Louis encephalitis virus strain Kern217, complete genome913195559%0.069%DQ525916.1Select seq gb|KF383045.1|Zika virus strain ArA27106 envelope protein gene, partial cds9069067%0.087%KF383045.1Select seq gb|KF383043.1|Zika virus strain ArA27443 envelope protein gene, partial cds9029027%0.087%KF383043.1Select seq gb|KF383035.1|Zika virus strain MR1429 envelope protein gene, partial cds9019017%0.086%KF383035.1Select seq gb|EF158067.1|St. Louis encephalitis virus strain BeAn 247377 polyprotein gene, partial cds899202664%0.069%EF158067.1Select seq gb|KF383103.1|Zika virus strain ArA986 nonstructural protein 5 gene, partial cds8978976%0.088%KF383103.1Select seq gb|KF383086.1|Zika virus strain ArA975 nonstructural protein 5 gene, partial cds8978976%0.088%KF383086.1Select seq gb|KF383041.1|Zika virus strain ArA27290 envelope protein gene, partial cds8978977%0.087%KF383041.1Select seq gb|KF383042.1|Zika virus strain ArA27096 envelope protein gene, partial cds8938937%0.086%KF383042.1Select seq gb|KF589299.1|St. Louis encephalitis virus strain FLU3632 polyprotein gene, complete cds886189064%0.069%KF589299.1Select seq gb|FJ753287.2|St. Louis encephalitis virus strain 79V-2533, complete genome881180563%0.069%FJ753287.2Select seq gb|KF383040.1|Zika virus strain ArA27101 envelope protein gene, partial cds8668667%0.086%KF383040.1Select seq gb|KF383104.1|Zika virus strain ArA982 nonstructural protein 5 gene, partial cds8658656%0.087%KF383104.1Select seq gb|KF383085.1|Zika virus strain ArD9957 nonstructural protein 5 gene, partial cds8658656%0.087%KF383085.1Select seq gb|KF383106.1|Zika virus strain ArA27443 nonstructural protein 5 gene, partial cds8618616%0.087%KF383106.1Select seq gb|KF383114.1|Zika virus strain AnD30332 nonstructural protein 5 gene, partial cds8568566%0.087%KF383114.1Select seq gb|KF383107.1|Zika virus strain ArA27407 nonstructural protein 5 gene, partial cds8568566%0.087%KF383107.1Select seq gb|KF383088.1|Zika virus strain ArD30101 nonstructural protein 5 gene, partial cds8568566%0.087%KF383088.1Select seq gb|KF383087.1|Zika virus strain ArD30156 nonstructural protein 5 gene, partial cds8528526%0.087%KF383087.1Select seq gb|KF383089.1|Zika virus strain ArD165531 nonstructural protein 5 gene, partial cds8388386%0.086%KF383089.1Select seq gb|KF383101.1|Zika virus strain ArD127710 nonstructural protein 5 gene, partial cds8348346%0.086%KF383101.1Select seq gb|KF383097.1|Zika virus strain ArD127994 nonstructural protein 5 gene, partial cds8348346%0.086%KF383097.1Select seq dbj|AB908162.1|Zika virus gene for polyprotein, partial cds, strain: ZIKV Hu/Tahiti/01u/2014NIID8348344%0.099%AB908162.1Select seq gb|KF383099.1|Zika virus strain ArD127987 nonstructural protein 5 gene, partial cds8298296%0.086%KF383099.1Select seq gb|KF383098.1|Zika virus strain ArD127988 nonstructural protein 5 gene, partial cds8258256%0.086%KF383098.1Select seq gb|KU872850.1|Zika virus isolate Dominican Rep-Rus-2016, NS2 partial cds8238234%0.099%KU872850.1

LOCUS KU991811 10643 bp RNA linear VRL 29-MAR-2016 DEFINITION Zika virus isolate Brazil/2016/INMI1 polyprotein gene, complete cds. ACCESSION KU991811 VERSION KU991811.1 GI:1012128960 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus. REFERENCE 1 (bases 1 to 10643) AUTHORS Carletti,F., Meschi,S., Castilletti,C., Zaccaro,P., Ferraioli,V., Quartu,S., Colavita,F., Corpolongo,A., Nicastri,E., Di Caro,A., Ippolito,G. and Capobianchi,M.R. TITLE Isolation of infectious Zika virus from sera in a traveller returning from Brazil to Italy JOURNAL Unpublished REFERENCE 2 (bases 1 to 10643) AUTHORS Carletti,F., Meschi,S., Castilletti,C., Zaccaro,P., Ferraioli,V., Quartu,S., Colavita,F., Corpolongo,A., Nicastri,E., Di Caro,A., Ippolito,G. and Capobianchi,M.R. TITLE Direct Submission JOURNAL Submitted (23-MAR-2016) Virology Laboratory, INMI L. Spallanzani, Via Portuense 292, Rome 00149, Italy COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10643 /organism="Zika virus" /mol_type="genomic RNA" /isolate="Brazil/2016/INMI1" /host="Homo sapiens" /db_xref="taxon:64320" /country="Italy: Rome" /collection_date="06-Mar-2016" CDS 74..10345 /codon_start=1 /product="polyprotein" /protein_id="AMS00611.1" /db_xref="GI:1012128961" /translation="MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPI RMVLAILAFLRFTAIKPSLGLINRWGSVGKKEAMEIIKKFKKDLAAMLRIINARKEKK RRGADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYIQI MDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGEARRSRRAVT LPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAIAWLLGSSTSQKV IYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIE LVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHET DENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWF HDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAE MDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAG TDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVG EKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFG AAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADVGC SVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSR MENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGK SYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLE CDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGI EESDLIIPKSLAGPLSHHNTREGYRTQMEGPWHSEELEIRFEECPGTKVHVEETCGTR GPSLRSTTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKEPESNLVRSMVTA GSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLA ILMGATFAEMNTGGDVAHLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQT AISALEGDLMVLINGFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGL ATCGGFMLLSLKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSE VLTAVGLICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKD AEVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGAWY VYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMW HVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGHSEVQLLAVPPGERARN IQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAIT QGRREEETPVDCFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKTRLRTVILAP TRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLY IMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEV PERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKT KHQEWDFVVTTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQR RGRIGRNPNKPGDEYLYGGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADK VAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIM EDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTL PGHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGIFFV LMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPEPEKQRSP QDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIGFSMDIDLRPA SAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMGKGMPFYAWDFGVPL LMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQKRTAAGIMKNPVVDGIV VTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWGWGEAGALITAATSTLWEGSP NKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNAGLVKRRGGGTGETLGEKWKARLNQ MSALEFYSYKKSGITEVCREEARRALKDGVATGGHAVSRGSAKLRWLVERGYLQPYGK VIDLGCGRGGWSYYAATIRKVQEVKGYTKGGPGHEEPVLVQSYGWNIVRLKSGVDVFH MAAEPCDTLLCDIGESSSSPEVEEARTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMM ETLERLQRRYGGGLVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRP VKYEEDVNLGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHG SYEAPTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVPD PQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEKEWKTAV EAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKAKGSRAIWYMWLGA RFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVLEEMSRIPGGRMYADDTAGWD TRISRFDLENEALITNQMEKGHRALALAIIKYTYQNKVVKVLRPAEKGKTVMDIISRQ DQRGSGQVVTYALNTFTNLVVQLIRNMEAEEVLEMQDLWLLRRSEKVTNWLQSNGWDR LKRMAVSGDDCVVKPIDDRFAHALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHH FNKLHLKDGRSIVVPCRHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRR DLRLMANAICSSVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKT PVTKWTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST QVRYLGEEGSTPGVL" ORIGIN 1 tcgagtttga agcgaaagct agcaacagta tcaacaggtt ttattttgga tttggaaacg 61 agagtttctg gtcatgaaaa acccaaaaaa gaaatccgga ggattccgga ttgtcaatat 121 gctaaaacgc ggagtagccc gtgtgagccc ctttgggggc ttgaagaggc tgccagccgg 181 acttctgctg ggtcatgggc ccatcaggat ggtcttggcg attctagcct ttttgagatt 241 cacggcaatc aagccatcac tgggtctcat caatagatgg ggttcagtgg ggaaaaaaga 301 ggctatggaa ataataaaga agttcaagaa agatctggct gccatgctga gaataatcaa 361 tgctaggaag gagaagaaga gacgaggcgc agatactagt gtcggaattg ttggcctcct 421 gctgaccaca gctatggcag cggaggtcac tagacgtggg agtgcatact atatgtactt 481 ggacagaaac gatgctgggg aggccatatc ttttccaacc acattgggga tgaataagtg 541 ttatatacag atcatggatc ttggacacat gtgtgatgcc accatgagct atgaatgccc 601 tatgctggat gagggggtgg aaccagatga cgtcgattgt tggtgcaaca cgacgtcaac 661 ttgggttgtg tacggaacct gccatcacaa aaaaggtgaa gcacggagat ctagaagagc 721 tgtgacgctc ccctcccatt ccactaggaa gctgcaaacg cggtcgcaaa cctggttgga 781 atcaagagaa tacacaaagc acttgattag agtcgaaaat tggatattca ggaaccctgg 841 cttcgcgtta gcagcagctg ccatcgcttg gcttttggga agctcaacga gccaaaaagt 901 catatacttg gtcatgatac tgctgattgc cccggcatac agcatcaggt gcataggagt 961 cagcaatagg gactttgtgg aaggtatgtc aggtgggact tgggttgatg ttgtcttgga 1021 acatgggggt tgtgtcaccg taatggcaca ggacaaaccg actgtcgaca tagagctggt 1081 tacaacaaca gtcagcaaca tggcggaggt aagatcctac tgctatgagg catcaatatc 1141 agacatggct tcggacagcc gctgcccaac acaaggtgaa gcctaccttg acaagcaatc 1201 agacactcaa tatgtctgca aaagaacgtt agtggacaga ggctggggaa atggatgtgg 1261 actttttggc aaagggagcc tggtgacatg cgctaagttt gcatgctcca agaaaatgac 1321 cgggaagagc atccagccag agaatctgga gtaccggata atgctgtcag ttcatggctc 1381 ccagcacagt gggatgatcg ttaatgacac aggacatgaa actgatgaga atagagcgaa 1441 ggttgagata acgcccaatt caccaagagc cgaagccacc ctggggggtt ttggaagcct 1501 aggacttgat tgtgaaccga ggacaggcct tgacttttca gatttgtatt acttgactat 1561 gaataacaag cactggttgg ttcacaagga gtggttccac gacattccat taccttggca 1621 cgctggggca gacaccggaa ctccacactg gaacaacaaa gaagcactgg tagagttcaa 1681 ggacgcacat gccaaaaggc aaactgtcgt ggttctaggg agtcaagaag gagcagttca 1741 cacggccctt gctggagctc tggaggctga gatggatggt gcaaagggaa ggctgtcctc 1801 tggccacttg aaatgtcgcc tgaaaatgga taaacttaga ttgaagggcg tgtcatactc 1861 cttgtgtacc gcagcgttca cattcaccaa gatcccggct gaaacactgc acgggacagt 1921 cacagtggag gtacagtacg cagggacaga tggaccttgc aaggttccag ctcagatggc 1981 ggtggacatg caaactctga ccccagttgg gaggttgata accgctaacc ccgtaatcac 2041 tgaaagcact gagaactcta agatgatgct ggaacttgat ccaccatttg gggactctta 2101 cattgtcata ggagtcgggg agaagaagat cacccaccac tggcacagga gtggcagcac 2161 cattggaaaa gcatttgaag ccactgtgag aggtgccaag agaatggcag tcttgggaga 2221 cacagcctgg gactttggat cagttggagg cgctctcaac tcattgggca agggcatcca 2281 tcaaattttt ggagcagctt tcaaatcatt gtttggagga atgtcctggt tctcacaaat 2341 tctcattgga acgttgctga tgtggttggg tctgaacaca aagaatggat ctatttccct 2401 tatgtgcttg gccttagggg gagtgttgat cttcttatcc acagccgtct ctgctgatgt 2461 ggggtgttcg gtggacttct caaagaagga gacgagatgt ggtacagggg tgttcgtcta 2521 taacgacgtt gaagcctgga gggacaggta caagtaccat cctgactccc cccgtagatt 2581 ggcagcagca gtcaagcaag cctgggaaga tggtatctgc gggatctcct ctgtttcaag 2641 aatggaaaat atcatgtgga gatcagtaga aggggagctc aacgcaatcc tggaagagaa 2701 tggagttcaa ctgacggtcg ttgtgggatc tgtaaaaaac cccatgtgga gaggtccaca 2761 gagattgccc gtgcctgtga acgagctgcc ccacggctgg aaggcttggg ggaaatcgta 2821 cttcgtcaga gcagcaaaga caaataacag ctttgtcgtg gatggtgaca cactgaagga 2881 atgcccactc aaacatagag catggaacag ctttcttgtg gaggatcatg ggttcggggt 2941 atttcacact agtgtctggc tcaaggttag agaagattat tcattagagt gtgatccagc 3001 cgttattgga acagctgtta agggaaagga ggctgtacac agtgatctag gctactggat 3061 tgagagtgag aagaatgaca catggaggct gaagagggcc catctgatcg agatgaaaac 3121 atgtgaatgg ccaaagtccc acacattgtg gacagatgga atagaagaga gtgatctgat 3181 catacccaag tctttagctg ggccactcag ccatcacaat accagagagg gctacaggac 3241 ccaaatggaa gggccatggc acagtgaaga gcttgaaatt cggtttgagg aatgcccagg 3301 cactaaggtc cacgtggagg aaacatgtgg aacaagagga ccatctctga gatcaaccac 3361 tgcaagcgga agggtgatcg aggaatggtg ctgcagggag tgcacaatgc ccccactgtc 3421 gttccgggct aaagatggct gttggtatgg aatggagata aggcccagga aagaaccaga 3481 aagcaactta gtaaggtcaa tggtgactgc aggatcaact gatcacatgg atcacttctc 3541 ccttggagtg cttgtgattc tgctcatggt gcaggaaggg ctgaagaaga gaatgaccac 3601 aaagatcatc ataagcacat caatggcagt gctggtagct atgatcctgg gaggattttc 3661 aatgagtgac ctggctaagc ttgcaatttt gatgggtgcc accttcgcgg aaatgaacac 3721 tggaggagat gtagctcatc tggcgctgat agcggcattc aaagtcagac cagcgttgct 3781 ggtatctttc atcttcagag ctaattggac accccgtgaa agcatgctgc tggccttggc 3841 ctcgtgtctt ttgcaaactg cgatctccgc cttggaaggc gacctgatgg ttctcatcaa 3901 tggttttgct ttggcctggt tggcaatacg agcgatggtt gttccacgca ctgacaacat 3961 caccttggca atcctggctg ctctgacacc actggcccgg ggcacactgc ttgtggcgtg 4021 gagagcaggc cttgctactt gcggggggtt tatgctcctc tctctgaagg gaaaaggcag 4081 tgtgaagaag aacttaccat ttgtcatggc cctgggacta accgctgtga ggctggtcga 4141 ccccatcaac gtggtgggac tgctgttgct cacaaggagt gggaagcgga gctggccccc 4201 tagcgaagta ctcacagctg ttggcctgat atgcgcattg gctggagggt tcgccaaggc 4261 agatatagag atggctgggc ccatggccgc ggtcggtctg ctaattgtca gttacgtggt 4321 ctcaggaaag agtgtggaca tgtacattga aagagcaggt gacatcacat gggaaaaaga 4381 tgcggaagtc actggaaaca gtccccggct cgatgtggcg ctagatgaga gtggtgattt 4441 ctccctggtg gaggatgacg gtccccccat gagagagatc atactcaagg tggtcctgat 4501 gaccatctgt ggcatgaacc caatagccat accctttgca gctggagcgt ggtacgtata 4561 cgtgaagact ggaaaaagga gtggtgctct atgggatgtg cctgctccca aggaagtaaa 4621 aaagggggag accacagatg gagtgtacag agtaatgact cgcagactgc taggttcaac 4681 acaagttgga gtgggagtta tgcaagaggg ggtctttcac actatgtggc acgtcacaaa 4741 aggatccgcg ctgagaagcg gtgaagggag acttgatcca tactggggag atgtcaagca 4801 ggatctggtg tcatactgtg gtccgtggaa gctagatgcc gcctgggacg ggcacagcga 4861 ggtgcagctc ttggccgtgc cccccggaga gagagcgagg aacatccaga ctctgcccgg 4921 aatatttaag acaaaggatg gggacattgg agcggttgcg ctggattacc cagcaggaac 4981 ttcaggatct ccaatcctag acaagtgtgg gagagtgata ggactttatg gcaatggggt 5041 cgtgatcaaa aatgggagtt atgttagtgc catcacccaa gggaggaggg aggaagagac 5101 tcctgttgat tgcttcgagc cttcgatgct gaagaagaag cagctaactg tcttagactt 5161 gcatcctgga gctgggaaaa ccaggagagt tcttcctgaa atagtccgtg aagccataaa 5221 aacaagactc cgtactgtga tcttagctcc aaccagggtt gtcgctgctg aaatggagga 5281 agcccttaga gggcttccag tgcgttatat gacaacagca gtcaatgtca cccactctgg 5341 aacagaaatc gtcgacttaa tgtgccatgc caccttcact tcacgtctac tacagccaat 5401 cagagtcccc aactataatc tgtatattat ggatgaggcc cacttcacag atccctcaag 5461 tatagcagca agaggataca tttcaacaag ggttgagatg ggcgaggcgg ctgccatctt 5521 catgaccgcc acgccaccag gaacccgtga cgcatttccg gactccaact caccaattat 5581 ggacaccgaa gtggaagtcc cagagagagc ctggagctca ggctttgatt gggtgacgga 5641 tcattctgga aaaacagttt ggtttgttcc aagcgtgagg aacggcaatg agatcgcagc 5701 ttgtctgaca aaggctggaa aacgggtcat acagctcagc agaaagactt ttgagacaga 5761 gttccagaaa acaaaacatc aagagtggga ctttgtcgtg acaactgaca tttcagagat 5821 gggcgccaac tttaaagctg accgtgtcat agattccagg agatgcctaa agccggtcat 5881 acttgatggc gagagagtca ttctggctgg acccatgcct gtcacacatg ccagcgctgc 5941 ccagaggagg gggcgcatag gcaggaatcc caacaaacct ggagatgagt atctgtatgg 6001 aggtgggtgc gcagagactg acgaagacca tgcacactgg cttgaagcaa gaatgctcct 6061 tgacaatatt tacctccaag atggcctcat agcctcgctc tatcgacctg aggccgacaa 6121 agtagcagcc attgagggag agttcaagct taggacggag caaaggaaga cctttgtgga 6181 actcatgaaa agaggagatc ttcctgtttg gctggcctat caggttgcat ctgccggaat 6241 aacctacaca gatagaagat ggtgctttga tggcacgacc aacaacacca taatggaaga 6301 cagtgtgccg gcagaggtgt ggaccagaca cggagagaaa agagtgctca aaccgaggtg 6361 gatggacgcc agagtttgtt cagatcatgc ggccctgaag tcattcaagg agtttgccgc 6421 tgggaaaaga ggagcggctt ttggagtgat ggaagccctg ggaacactgc caggacacat 6481 gacagagaga ttccaggaag ccattgacaa cctcgctgtg ctcatgcggg cagagactgg 6541 aagcaggcct tacaaagccg cggcggccca attgccggag accctagaga ccattatgct 6601 tttggggttg ctgggaacag tctcgctggg aatctttttc gtcttgatga ggaacaaggg 6661 catagggaag atgggctttg gaatggtgac tcttggggcc agcgcatggc tcatgtggct 6721 ctcggaaatt gagccagcca gaattgcatg tgtcctcatt gttgtgttcc tattgctggt 6781 ggtgctcata cctgagccag aaaagcaaag atctccccag gacaaccaaa tggcaatcat 6841 catcatggta gcagtaggtc ttctgggctt gattaccgcc aatgaactcg gatggttgga 6901 gagaacaaag agtgacctaa gccatctaat gggaaggaga gaggaggggg caaccatagg 6961 attctcaatg gacattgacc tgcggccagc ctcagcttgg gccatctatg ctgccttgac 7021 aactttcatt accccagccg tccaacatgc agtgaccact tcatacaaca actactcctt 7081 aatggcgatg gccacgcaag ctggagtgtt gtttggtatg ggcaaaggga tgccattcta 7141 cgcatgggac tttggagtcc cgctgctaat gataggttgc tactcacaat taacacccct 7201 gaccctaata gtggccatca ttttgctcgt ggcgcactac atgtacttga tcccagggct 7261 gcaggcagca gctgcgcgtg ctgcccagaa gagaacggca gctggcatca tgaagaaccc 7321 tgttgtggat ggaatagtgg tgactgacat tgacacaatg acaattgacc cccaagtgga 7381 gaaaaagatg ggacaggtgc tactcatagc agtagccgtc tccagcgcca tactgtcgcg 7441 gaccgcctgg gggtgggggg aggctggggc cctgatcaca gccgcaactt ccactttgtg 7501 ggaaggctct ccgaacaagt actggaactc ctctacagcc acttcactgt gtaacatttt 7561 taggggaagt tacttggctg gagcttctct aatctacaca gtaacaagaa acgctggctt 7621 ggtcaagaga cgtgggggtg gaacaggaga gaccctggga gagaaatgga aggcccgctt 7681 gaaccagatg tcggccctgg agttctactc ctacaaaaag tcaggcatca ccgaggtgtg 7741 cagagaagag gcccgccgcg ccctcaagga cggtgtggca acgggaggcc atgctgtgtc 7801 ccgaggaagt gcaaagctga gatggttggt ggagcgggga tacctgcagc cctatggaaa 7861 ggtcattgat cttggatgtg gcagaggggg ctggagttac tacgccgcca ccatccgcaa 7921 agttcaagaa gtgaaaggat acacaaaagg aggccctggt catgaagaac ccgtgttggt 7981 gcaaagctat gggtggaaca tagtccgtct taagagtggg gtggacgtct ttcatatggc 8041 ggctgagccg tgtgacacgt tgctgtgtga cataggtgag tcatcatcta gtcctgaagt 8101 ggaagaagca cggacgctca gagtcctctc catggtgggg gattggcttg aaaaaagacc 8161 aggagccttt tgtataaaag tgttgtgccc atacaccagc actatgatgg aaaccctgga 8221 gcgactgcag cgtaggtatg ggggaggact ggtcagagtg ccactctccc gcaactctac 8281 acatgagatg tactgggtct ctggagcgaa aagcaacacc ataaaaagtg tgtccaccac 8341 gagccagctc ctcttggggc gcatggacgg gcctaggagg ccagtgaaat atgaggagga 8401 tgtgaatctc ggctctggca cgcgggctgt ggtaagctgc gctgaagctc ccaatatgaa 8461 gatcattggt aaccgcattg aaaggatccg cagtgagcac gcggaaacgt ggttctttga 8521 cgagaaccac ccatatagga catgggctta ccatggaagc tatgaggccc ccacacaagg 8581 gtcagcgtcc tctctaataa acggggttgt caggctcctg tcaaaaccct gggatgtggt 8641 gactggagtc acaggaatag ccatgaccga caccacaccg tatggtcagc aaagagtttt 8701 caaggaaaaa gtggacacta gggtgccaga cccccaagaa ggcactcgtc aggttatgag 8761 catggtctct tcctggttgt ggaaagagct aggcaaacac aaacggccac gagtctgtac 8821 caaagaagag ttcatcaaca aggttcgtag caatgcagca ttaggggcaa tatttgaaga 8881 ggaaaaagag tggaagactg cagtggaagc tgtgaacgat ccaaggttct gggctctagt 8941 ggacaaggaa agagagcacc acctgagagg agagtgccag agttgtgtgt acaacatgat 9001 gggaaaaaga gaaaagaaac aaggggaatt tggaaaggcc aagggcagcc gcgccatctg 9061 gtatatgtgg ctaggggcta gatttctaga gttcgaagcc cttggattct tgaacgagga 9121 tcactggatg gggagagaga actcaggagg tggtgttgaa gggctgggat tacaaagact 9181 cggatatgtc ctagaagaga tgagtcgcat accaggagga aggatgtatg cagatgacac 9241 tgctggctgg gatacccgca tcagcaggtt tgatctggag aatgaagctc taatcaccaa 9301 ccaaatggag aaagggcaca gggccttggc attggccata atcaagtaca cataccaaaa 9361 caaagtggta aaggtcctta gaccagctga aaaagggaaa acagttatgg acattatttc 9421 gagacaagac caaaggggga gcggacaagt tgtcacttac gctcttaaca catttaccaa 9481 cctagtggtg caactcattc ggaatatgga ggctgaggaa gttctagaga tgcaagactt 9541 gtggctgctg cggaggtcag agaaagtgac caactggttg cagagcaacg gatgggatag 9601 gctcaaacga atggcagtca gtggagatga ttgcgttgtg aagccaattg atgataggtt 9661 tgcacatgcc ctcaggttct tgaatgatat gggaaaagtt aggaaggaca cacaagagtg 9721 gaaaccctca actggatggg acaactggga agaagttccg ttttgctccc accacttcaa 9781 caagctccat ctcaaggacg ggaggtccat tgtggttccc tgccgccacc aagatgaact 9841 gattggccgg gcccgcgtct ctccaggggc gggatggagc atccgggaga ctgcttgcct 9901 agcaaaatca tatgcgcaga tgtggcagct cctttatttc cacagaaggg acctccgact 9961 gatggccaat gccatttgtt catctgtgcc agttgactgg gttccaactg ggagaactac 10021 ctggtcaatc catggaaagg gagaatggat gaccactgaa gacatgcttg tggtgtggaa 10081 cagagtgtgg attgaggaga acgaccacat ggaagacaag accccagtta cgaaatggac 10141 agacattccc tatttgggaa aaagggaaga cttgtggtgt ggatctctca tagggcacag 10201 accgcgcacc acctgggctg agaacattaa aaacacagtc aacatggtgc gcaggatcat 10261 aggtgatgaa gaaaagtaca tggactacct atccacccaa gttcgctact tgggtgaaga 10321 agggtctaca cctggagtgc tgtaagcacc aatcttaatg ttgtcaggcc tgctagtcag 10381 ccacagcttg gggaaagctg tgcagcctgt gaccctccca ggagaagctg ggaaaccaag 10441 cctatagtca ggccgagaac gccatggcac ggaagaagcc atgctgcctg tgagcccctc 10501 agaggacact gagtcaaaaa accccacgcg cttggaggcg caggatggga aaagaaggtg 10561 gcgaccttcc ccacccttca atctggggcc tgaactggag atcagctgtg gatctccaga 10621 agagggacta gtggttagag gag

Zika virus isolate Brazil/2016/INMI1 polyprotein gene, complete cdsGenBank: KU991811.1 http://www.ncbi.nlm.nih.gov/nucleotide/1012128960?report=genbank&log$=nucltop&blast_rank=29&RID=FU55MP1C014

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FUTURE OUTLOOK AND DIRECTIONSThe current incidence of Zika virus infection in the Americas is difficult to gauge because the symptoms are nonspecific and generally mild, laboratory diagnosis is not uniformly available, and flavivirus antibody cross-reactivity complicates serologic assessment in areas in which dengue is endemic. Nevertheless, given the historically high incidence of dengue in the region and the recent experience with the chikungunya virus in the Americas, millions of Zika virus infections should be expected as the virus continues to spread.132-135 If Brazil serves as a bellwether for the rest of Latin America and the Caribbean, substantial numbers of infants with microcephaly and other adverse pregnancy outcomes could be identified in the upcoming months. The potential burden of illness from Guillain–Barré syndrome is hard to assess, given the difficulties with serologic diagnosis in areas where dengue is endemic and the paucity of published data on current incidence. The underlying reasons for the emergence of Zika virus in the past decade are unknown. Recent global increases in the incidence and spread of dengue, chikungunya, and now Zika virus — all with A. aegypti as the primary vector — suggest common underlying mechanisms for their emergence, such as globalization and urbanization.132,136 Other possible explanations include viral mutations affecting transmission or virulence and viral introduction to previously unexposed populations leading to epidemic spread. Further research will be required to determine whether the recently observed associations with adverse birth outcomes and Guillain–Barré syndrome simply reflect an increased incidence of infection or whether they result from a change in viral virulence. In areas of Africa and Asia where Zika virus is endemic, the incidence of infection, whether outbreaks will occur, and the reason for the previous lack of recorded cases of adverse pregnancy outcomes or Guillain–Barré syndrome are unknown. It is possible that many exposures occur in children, in whom Guillain–Barré syndrome may be less likely to develop and who would later be immune to infections during pregnancy. The long-term outlook with regard to the current Zika virus outbreak in the Americas is uncertain. Herd immunity sufficient to slow further transmission will undoubtedly occur, although this will not obviate the need for immediate and long-term prevention and control strategies. Whether and where the virus becomes endemic and whether an enzootic transmission cycle will develop somewhere in the Americas are matters of conjecture, but they are of considerable importance for the long-term development and sustainability of countermeasures, such as a Zika virus vaccine. What is clear is the need to rapidly and systematically address identified research gaps. These include a complete understanding of the frequency and full spectrum of clinical outcomes resulting from fetal Zika virus infection and of the environmental factors that influence emergence, as well as the development of discriminating diagnostic tools for flaviviruses, animal models for fetal developmental effects due to viral infection, new vector control products and strategies, effective therapeutics, and vaccines to protect humans against the disease.