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Pennsylvania Blood Tests Submitted for Zika Testing as of March 28, 2016 Information updated Mondays at 2 p.m.CDC Confirmed Cases: 11Pending Test Results: 211 http://www.health.pa.gov/My Health/Diseases and Conditions/U-Z/Pages/Zika.aspx#.VvlweeIrJgL

Pennsylvania Blood Tests Submitted for Zika Testing as of March 28, 2016 Information updated Mondays at 2 p.m.CDC Confirmed Cases: 11Pending Test Results: 211

Health Department investigating possible travel-related Zika, dengue cases on OahuSaturday, March 26th 2016, 8:25 pm EDTSaturday, March 26th 2016, 9:18 pm EDTBy Ben Gutierrez, Reporter / Weather AnchorCONNECT (Image: Hawaii News Now/File) (Image: Hawaii News Now)HONOLULU (HawaiiNewsNow) -The state Health Department said it is investigating four cases of travel-related mosquito borne viruses on Oahu. In a news release, the department said the cases include an individual who traveled to Latin America and returned to Hawaii ill. That person is being tested for dengue and Zika. There are other individuals who are being tested for dengue fever and chikungunya and have a history of travel to the Pacific Islands and Latin American. The department said all of the individuals have recovered. Officials said initial lab tests for the first individual were not conclusive, but further testing will be done by the Centers for Disease Control and Prevention. The state's vector control teams have been assessing residential and other areas in urban Honolulu and along the North Shore during the past week to determine if mosquito control measures are necessary. The department said those measures would include eliminating mosquito breeding sites or spraying to reduce adult mosquito activity. Dengue fever, Zika and ciungunya are all mosquito borne viruses that are spread when a sick person is bitten by a mosquito, which later bites another person. Evidence suggests that Zika can also be transmitted through unprotected sexual contact with someone who has been infected. There have been two confirmed Zika cases in Hawaii, including one earlier this week involving a Kauai resident who recently traveled to Latin America. The first confirmed Zika case was reported on Oahu in early March. That person is no longer infections, according to state officials. http://www.hawaiinewsnow.com/story/31573017/health-department-investigating-possible-zika-dengue-cases-on-oahu?utm_source=twitterfeed&utm_medium=twitter

HONOLULU (AP) — Health officials are reminding Hawaii residents to get rid of mosquito-breeding grounds as the state Department of Health investigates whether four reports of travel-related mosquito-borne illness on Oahu could be the Zika virus, dengue fever or chikungunya. The four cases were reported to the Department of Health on Thursday and all were acquired in Latin America or other Pacific Islands. Department of Health Director Virginia Pressler said there have been no locally-acquired mosquito-borne illnesses on Oahu. The four cases are no longer active. “The department is working closely with the counties and taking precautionary measures to respond to all suspected and confirmed cases,” Pressler said. “We ask for the public’s help in reducing mosquito breeding areas around homes and workplaces and preventing mosquito bites by using repellant or protective clothing.” It may take the Centers for Disease Control and Prevention months to identify the samples from Hawaii. Zika and dengue fever are spread by the same type of mosquito. To prevent mosquitoes from breeding, residents are encouraged to eliminate standing water from their homes, fix leaky outdoor faucets, clean gutters and treat plants that hold water. Honolulu Mayor Kirk Caldwell said following the state’s “Fight the Bite” campaign is important to stop the spread of what could become a serious health issue on the island. “If you feel ill with flu-like symptoms, please seek medical attention, especially if you’ve traveled to another part of the world recently,” he said. Honolulu officials are inspecting all city facilities for standing water and working to prevent mosquitoes from breeding.

HONOLULU (AP) — Health officials are reminding Hawaii residents to get rid of mosquito-breeding grounds as the state Department of Health investigates whether four reports of travel-related mosquito-borne illness on Oahu could be the Zika virus, dengue fever or chikungunya. The four cases were reported to the Department of Health on Thursday and all were acquired in Latin America or other Pacific Islands. Department of Health Director Virginia Pressler said there have been no locally-acquired mosquito-borne illnesses on Oahu. The four cases are no longer active. http://hawaiitribune-herald.com/news/state/officials-investigate-possible-zika-virus-cases-oahu

Alabama Residents Tested for Zika Virus as of March 28, 2016 Number Tested PositiveNumber of SubmissionsNumber with Results Pending3 38 16

Zika Virus – March 28, 2016 Texas has had 27 confirmed cases of Zika virus disease. Of those, 26 were in travelers who were infected abroad and diagnosed after they returned home; one of those travelers was a pregnant woman. One case involved a Dallas County resident who had sexual contact with someone who acquired the Zika infection while traveling abroad. Case counts by county: Bexar – 3 Dallas – 4 Fort Bend – 2 Grayson – 1 Harris – 11 Tarrant – 3 Travis – 2 Wise – 1

Map update https://www.google.com/maps/d/edit?hl=en&hl=en&authuser=0&authuser=0&mid=zv94AJqgUct4.kT4qLMXp3SLU

Zika Virushttps://www.odh.ohio.gov/odhprograms/bid/zdp/diseases/zika.aspx Zika virus cases in Ohio:

Zika VirusZika virus cases in Ohio:

Health Outbreak of microcephaly Zika virus Healthcare without Neura 28.03.2016 at 00:30 Experts explain increase in severe cases of microcephaly, 'Zika is thirsting for nervous system' Calcification in the brain and cerebrospinal fluid accumulation in the skull have reached increasingly babies R7 Home Get News On Your Mobile Text: -A + A From R7 * Brazil has already registered 907 cases of babies with microcephaly until March 19 Brazil has already registered 907 cases of babies with microcephaly until March 19 Eduardo Enomoto / R7 "OK, we care, will do what?". With a helpless sigh, Jaqueline Oliveira, 24, told R7 as it was dealing with the fact that his daughter Laura, just over three months, was born with microcephaly the São Paulo, the coastal city of Santos, is just one of the pictures that is the situation of hundreds of mothers in Brazil -. and, why not, of so many thousands who expect an accurate diagnosis of what affects the development of their children. According to the latest bulletin of the Ministry of Health, Brazil recorded 907 confirmed cases of microcephaly until 19 March and investigates other 4,293 suspected cases. The increase in cases of the disease was so intense since May 2015 - the month in which it was discovered the first case of zika in Brazil - the anomaly is now considered an outbreak. According to Mantovani and Marilisia Mardjane Lemos experts before zika epidemic, microcephaly was a relatively rare disease. Today, every week, the number of confirmed cases increases. Marilisia, a neurologist at the Brazilian Academy of Neurology, said that although the WHO (World Health Organization) have not confirmed the relationship of the disease with the new virus, it is undeniable: - The cases of microcephaly May grew absurdly here, and are being detected, mostly in infants whose mothers were infected zika. "The zika is thirsty by the nervous system." It was with this statement that the infectious disease Mardjane Lemos, coordinator of ambulatory Hélvio Hospital High Maceio - reference in cases of microcephaly related to the virus - began to tell as the frames of the disease began to worsen after the epidemic. Despite the 2416 km separating Campinas (in São Paulo state) - where it operates Marilisia - and Maceió, city Mardjane, the experiences of both experts showed convergent and important point: when mothers take zika virus in the first trimester of pregnancy the chances of the baby being born with severe microcephaly frames are much higher. This is because the fetus is still in formation, so that the virus has a much more potent effect on their development. According Marilisia, the most aggressive frame of the disease may have greater brain calcification, and increased cerebrospinal fluid in the skull. The expert explained that in normal children, this liquid is constantly released and reabsorbed by the brain, but with virus infection, this process ends up being stopped - and the cerebrospinal fluid builds up. - It is because of this that many children are born with the size of the head in the pattern, but with the skull taken by cerebrospinal fluid, and most of the calcified brain. This anomaly is probably also caused by zika and can take the baby to death. We do not consider the hidranocefalia a type of microcephaly because the size of the head is not reduced; but it is, rather, one of the effects caused by infection of the neurological system. WHO: vaccine zika virus can not get the fight outbreak The increase in cases of high gravity also drew attention to conditions in which the symptoms are milder - which typically occur when the mother is infected zika already in the last months of pregnancy. In such cases, the baby does not show reduced brain perimeter or hidranocefalia, but still has a delay in development - manifested by blindness, hearing problems, difficulty getting around and epilepsy. Therapies and ways to reduce the symptoms of the disease How advanced it is medicine, there is no cure for microcephaly. According Marilisia, what you can do are therapies that help in the development of the child. However, according to her, the service that the NHS (National Health System) offers patients is currently insignificant. - Children are years in line, waiting for these types of therapy. When we finally succeed, they are offered a poor and inadequate service. If it was just before the zika - when the number of cases was small - imagine what is not now. Mardjane agrees with the position of Marilisia, adding that there must be a preparation of doctors before telling parents that they will have a baby with cerebral malformation. - If the microcephaly is diagnosed during pregnancy by ultrasound examination, doctors must prepare parents. However, this usually does not occur because as the change in size of the head is not so apparent in the womb, these doctors are afraid to give an early diagnosis and make a mistake. See what was discovered and what is not known about the virus zika detected cases in Brazil Although 745 cases have been confirmed by the Ministry of Health, the experts believe that the reported number is much lower than the actual number of cases in Brazil. According Marilisia, microcephaly not need compulsory notification before zika epidemic and therefore there are high chances of the number extrapolate released by Folder: - The microcephaly never required mandatory reporting because it was [a disease] rare and not contagious. Thus, doctors did not need to report cases to government authorities, implying the absence of a national register for the disease. The neurologist said that most cases began to be reported in December 2015, due to the attention that the media started to give to zika epidemic and the relationship with the disease. - The records are beginning to be made now, so there are probably more cases of microcephaly than what is being reported. In 33 years of practice, already I followed thousands of patients, and none of them was never notified. Dissatisfaction of experts on the measures taken by the public health is great. When asked about the possibility of the country deal with this new crisis - which is far from over - both agreed spontaneously: - Brazil is not prepared. Other side Sought by R7, the press office of the Ministry of Health said that the recommendation given to health departments is that are recorded and passed on all cases of microcephaly. Regarding to stimulation therapy services to the development of children with malformation, the Pulp announced that there are now, throughout Brazil, 1,544 rehabilitation services, which make early stimulation of children between zero and three years. Moreover, it was stated that 11 centers are in the process of construction and should be completed by the end of this semester. According to the Ministry of Health, to fund these services are annually invested R $ 650 million. * Collaborated: Talyta Vespa, the R7 intern http://linkis.com/noticias.r7.com/saud/c7Uq6

Phylogenetic tree of full Zika sequences at Genbank with Haiti sequence labeled Haiti/1225/2014, ealiiest Brazil sequence labeled ZikaSPH2015, and French Polynesia sequence labeled H/PF/2013_French_Polynesia

Sequences producing significant alignments:Select:AllNone Selected:0 AlignmentsDownloadGenBankGraphicsDistance tree of resultsShow/hide columns of the table presenting sequences producing significant alignmentsSequences producing significant alignments:Select for downloading or viewing reportsDescriptionMax scoreTotal scoreQuery coverE valueIdentAccessionSelect seq gb|KU509998.1|Zika virus strain Haiti/1225/2014, complete genome1852518525100%0.0100%KU509998.1Select seq gb|KU321639.1|Zika virus strain ZikaSPH2015, complete genome1850218502100%0.099%KU321639.1Select seq gb|KJ776791.1|Zika virus strain H/PF/2013 polyprotein gene, complete cds1844718447100%0.099%KJ776791.1Select seq gb|KU729218.1|Zika virus isolate BeH828305 polyprotein gene, complete cds1842018420100%0.099%KU729218.1Select seq gb|KU707826.1|Zika virus isolate SSABR1, complete genome1842018420100%0.099%KU707826.1Select seq gb|KU365779.1|Zika virus strain BeH819966 polyprotein gene, complete cds1842018420100%0.099%KU365779.1Select seq gb|KU926309.1|Zika virus isolate Rio-U1, complete genome1840818408100%0.099%KU926309.1Select seq gb|KU365780.1|Zika virus strain BeH815744 polyprotein gene, complete cds1840818408100%0.099%KU365780.1Select seq gb|KU940228.1|Zika virus isolate Bahia07, partial genome1840218402100%0.099%KU940228.1Select seq gb|KU527068.1|Zika virus strain Natal RGN, complete genome1840218402100%0.099%KU527068.1Select seq gb|KU647676.1|Zika virus strain MRS_OPY_Martinique_PaRi_2015 polyprotein gene, complete cds1840218402100%0.099%KU647676.1Select seq gb|KU365777.1|Zika virus strain BeH818995 polyprotein gene, complete cds1840218402100%0.099%KU365777.1Select seq gb|KU501217.1|Zika virus strain 8375 polyprotein gene, complete cds1839918399100%0.099%KU501217.1Select seq gb|KU497555.1|Zika virus isolate Brazil-ZKV2015, complete genome183951839599%0.099%KU497555.1Select seq gb|KU729217.2|Zika virus isolate BeH823339 polyprotein gene, complete cds1839318393100%0.099%KU729217.2Select seq gb|KU501216.1|Zika virus strain 103344 polyprotein gene, complete cds1839318393100%0.099%KU501216.1Select seq gb|KU820897.1|Zika virus isolate FLR polyprotein gene, complete cds1839018390100%0.099%KU820897.1Select seq gb|KU365778.1|Zika virus strain BeH819015 polyprotein gene, complete cds1839018390100%0.099%KU365778.1Select seq gb|KU312312.1|Zika virus isolate Z1106033 polyprotein gene, complete cds1839018390100%0.099%KU312312.1Select seq gb|KU922960.1|Zika virus isolate MEX/InDRE/Sm/2016, complete genome1838418384100%0.099%KU922960.1Select seq gb|KU926310.1|Zika virus isolate Rio-S1, complete genome1838118381100%0.099%KU926310.1Select seq gb|KU922923.1|Zika virus isolate MEX/InDRE/Lm/2016, complete genome1838118381100%0.099%KU922923.1Select seq gb|KU501215.1|Zika virus strain PRVABC59, complete genome1838118381100%0.099%KU501215.1Select seq gb|KU870645.1|Zika virus isolate FB-GWUH-2016, complete genome1837518375100%0.099%KU870645.1Select seq gb|KU853013.1|Zika virus isolate Dominican Republic/2016/PD2, complete genome1837218372100%0.099%KU853013.1Select seq gb|KU853012.1|Zika virus isolate Dominican Republic/2016/PD1, complete genome1837018370100%0.099%KU853012.1Select seq gb|KU820898.1|Zika virus isolate GZ01 polyprotein gene, complete cds1836318363100%0.099%KU820898.1Select seq gb|KU955590.1|Zika virus isolate Z16019 polyprotein gene, complete cds1835718357100%0.099%KU955590.1Select seq gb|KU740184.2|Zika virus isolate GD01 polyprotein gene, complete cds1835418354100%0.099%KU740184.2Select seq gb|KU761564.1|Zika virus isolate GDZ16001 polyprotein gene, complete cds1835418354100%0.099%KU761564.1Select seq gb|KU955589.1|Zika virus isolate Z16006 polyprotein gene, complete cds1834518345100%0.099%KU955589.1Select seq gb|KU820899.2|Zika virus isolate ZJ03, complete genome1834518345100%0.099%KU820899.2Select seq gb|KU940224.1|Zika virus isolate Bahia09, partial genome183091830999%0.099%KU940224.1Select seq gb|KU744693.1|Zika virus isolate VE_Ganxian, complete genome1825818258100%0.099%KU744693.1Select seq gb|KU866423.1|Zika virus isolate Zika virus/SZ01/2016 polyprotein gene, complete cds1823618236100%0.099%KU866423.1Select seq gb|KU681081.3|Zika virus isolate Zika virus/H.sapiens-tc/THA/2014/SV0127- 14, complete genome1810518105100%0.099%KU681081.3Select seq gb|KU955593.1|Zika virus isolate Zika virus/H.sapiens-tc/KHM/2010/FSS13025, complete genome1779817798100%0.098%KU955593.1Select seq gb|JN860885.1|Zika virus isolate FSS13025 polyprotein gene, partial cds177981779899%0.098%JN860885.1Select seq gb|KF993678.1|Zika virus strain PLCal_ZV from Canada polyprotein gene, partial cds177511775198%0.099%KF993678.1Select seq gb|EU545988.1|Zika virus polyprotein gene, complete cds1764317643100%0.098%EU545988.1Select seq gb|KU681082.3|Zika virus isolate Zika virus/H.sapiens-tc/PHL/2012/CPC-0740, complete genome1748817488100%0.098%KU681082.3Select seq gb|HQ234499.1|Zika virus isolate P6-740 polyprotein gene, partial cds164661646699%0.096%HQ234499.1Select seq gb|KF268949.1|Zika virus isolate ARB15076 polyprotein gene, complete cds1328413284100%0.089%KF268949.1Select seq gb|KF268948.1|Zika virus isolate ARB13565 polyprotein gene, complete cds1328413284100%0.089%KF268948.1Select seq gb|KU720415.1|Zika virus strain MR 766 polyprotein gene, complete cds1328113281100%0.089%KU720415.1Select seq gb|KF383115.1|Zika virus strain ArB1362 polyprotein gene, complete cds1327913279100%0.089%KF383115.1Select seq gb|KF268950.1|Zika virus isolate ARB7701 polyprotein gene, complete cds1327713277100%0.089%KF268950.1Select seq gb|HQ234498.1|Zika virus isolate MR_766 polyprotein gene, partial cds132751327599%0.089%HQ234498.1Select seq gb|KU955595.1|Zika virus isolate Zika virus/A.taylori-tc/SEN/1984/41671-DAK, complete genome1327213272100%0.089%KU955595.1Select seq gb|DQ859059.1|Zika virus strain MR 766 polyprotein gene, complete cds1326613266100%0.089%DQ859059.1Select seq gb|KU955592.1|Zika virus isolate Zika virus/A.taylori-tc/SEN/1984/41662-DAK, complete genome1326313263100%0.089%KU955592.1Select seq gb|KF383119.1|Zika virus strain ArD158084 polyprotein gene, complete cds1326313263100%0.089%KF383119.1Select seq dbj|LC002520.1|Zika virus genomic RNA, complete genome, strain: MR766-NIID1325913259100%0.089%LC002520.1Select seq gb|KU955591.1|Zika virus isolate Zika virus/A.africanus-tc/SEN/1984/41525-DAK, complete genome1324513245100%0.089%KU955591.1Select seq gb|KU955594.1|Zika virus isolate Zika virus/M.mulatta-tc/UGA/1947/MR-766, complete genome1324313243100%0.089%KU955594.1Select seq gb|KF383116.1|Zika virus strain ArD7117 polyprotein gene, complete cds1322113221100%0.089%KF383116.1Select seq gb|HQ234501.1|Zika virus isolate ArD_41519 polyprotein gene, partial cds132141321499%0.089%HQ234501.1Select seq gb|AY632535.2|Zika virus strain MR 766, complete genome1320013200100%0.089%AY632535.2Select seq gb|KF383117.1|Zika virus strain ArD128000 polyprotein gene, complete cds1314213142100%0.088%KF383117.1Select seq gb|HQ234500.1|Zika virus isolate IbH_30656 polyprotein gene, partial cds131351313599%0.088%HQ234500.1Select seq gb|KF383118.1|Zika virus strain ArD157995 polyprotein gene, complete cds1294013008100%0.088%KF383118.1Select seq gb|KF383121.1|Zika virus strain ArD158095 polyprotein gene, partial cds128611286197%0.089%KF383121.1Select seq gb|KF383120.1|Zika virus strain ArD142623 nonfunctional polyprotein gene, partial sequence108441084497%0.084%KF383120.1Select seq gb|KU940227.1|Zika virus isolate Bahia08, partial genome50651448087%0.095%KU940227.1Select seq gb|KU312314.1|Zika virus isolate Z1106031 polyprotein gene, partial cds4985498527%0.099%KU312314.1Select seq gb|KU312313.1|Zika virus isolate Z1106032 polyprotein gene, partial cds4958495827%0.099%KU312313.1Select seq gb|KU646828.1|Zika virus isolate Si322 polyprotein gene, partial cds4664466425%0.099%KU646828.1Select seq gb|KU646827.1|Zika virus isolate Si323 polyprotein gene, partial cds4655465525%0.099%KU646827.1Select seq gb|KU312315.1|Zika virus isolate Z1106027 polyprotein gene, partial cds3443344318%0.099%KU312315.1Select seq gb|KU740199.1|Zika virus isolate VE_Ganxian2016 polyprotein gene, partial cds3220322017%0.099%KU740199.1Select seq gb|DQ859064.1|Spondweni virus strain SM-6 V-1 polyprotein gene, complete cds2879419795%0.071%DQ859064.1Select seq gb|KJ634273.1|Zika virus strain CK-ISL 2014 E protein (E) gene, partial cds2700270014%0.099%KJ634273.1Select seq gb|KU686218.1|Zika virus isolate MEX/InDRE/14/2015 polyprotein gene, partial cds2057205711%0.099%KU686218.1Select seq gb|KU179098.1|Zika virus isolate JMB-185 nonstructural protein 5 gene, partial cds2030203011%0.099%KU179098.1Select seq gb|KM078936.1|Zika virus strain CHI1410214 NS5 protein gene, partial cds176117619%0.0100%KM078936.1Select seq gb|KM078961.1|Zika virus strain CHI2612114 NS5 protein gene, partial cds175717579%0.099%KM078961.1Select seq gb|KM078930.1|Zika virus strain CHI2283714 NS5 protein gene, partial cds175517559%0.099%KM078930.1Select seq gb|KM078971.1|Zika virus strain CHI2613014 NS5 protein gene, partial cds175417549%0.099%KM078971.1Select seq gb|KM078970.1|Zika virus strain CHI2490414 NS5 protein gene, partial cds175417549%0.099%KM078970.1Select seq gb|KM078933.1|Zika virus strain CHI1058514 NS5 protein gene, partial cds175417549%0.099%KM078933.1Select seq gb|KM078929.1|Zika virus strain CHI1805214 NS5 protein gene, partial cds175217529%0.099%KM078929.1Select seq gb|KJ873160.1|Zika virus isolate NC14-03042014-3481 nonstructural protein 5 gene, partial cds161116118%0.0100%KJ873160.1Select seq gb|KJ873161.1|Zika virus isolate NC14-02042014-3220 nonstructural protein 5 gene, partial cds142914297%0.0100%KJ873161.1Select seq gb|KM851039.1|Zika virus strain SV0127/14 nonstructural protein 5 gene, partial cds139113917%0.099%KM851039.1Select seq gb|KM851038.1|Zika virus strain CPC-0740 nonstructural protein 5 gene, partial cds135513557%0.098%KM851038.1Select seq gb|KU556802.1|Zika virus isolate MEX/InDRE/14/2015 NS5 protein gene, partial cds134613467%0.099%KU556802.1Select seq gb|AF013415.1|Zika virus strain MR-766 NS5 protein (NS5) gene, partial cds1306130610%0.088%AF013415.1Select seq gb|KT200609.1|Zika virus isolate BR/949/15 NS5 gene, partial cds124512456%0.099%KT200609.1Select seq gb|KU232300.1|Zika virus isolate 067ZV_PEBR15 NS5 protein gene, partial cds124012406%0.099%KU232300.1Select seq gb|KU232290.1|Zika virus isolate 036ZV_PEBR15 NS5 protein gene, partial cds123112316%0.099%KU232290.1Select seq gb|KU232297.1|Zika virus isolate 049ZV_PEBR15 NS5 protein gene, partial cds122912296%0.099%KU232297.1Select seq gb|KU232294.1|Zika virus isolate 061ZV_PEBR15 NS5 protein gene, partial cds122012206%0.099%KU232294.1Select seq gb|KU232292.1|Zika virus isolate 054ZV_PEBR15 NS5 protein gene, partial cds121812186%0.099%KU232292.1Select seq gb|KU232298.1|Zika virus isolate 050ZV_PEBR15 NS5 protein gene, partial cds121412146%0.099%KU232298.1Select seq gb|KU232296.1|Zika virus isolate 045ZV_PEBR15 NS5 protein gene, partial cds121112116%0.099%KU232296.1Select seq gb|KU232293.1|Zika virus isolate 057ZV_PEBR15 NS5 protein gene, partial cds121112116%0.099%KU232293.1Select seq gb|KU232295.1|Zika virus isolate 068ZV_PEBR15 NS5 protein gene, partial cds120512056%0.099%KU232295.1Select seq gb|KU232288.1|Zika virus isolate 001ZV_PEBR15 NS5 protein gene, partial cds119511956%0.099%KU232288.1Select seq gb|KU232289.1|Zika virus isolate 020ZV_PEBR15 NS5 protein gene, partial cds119111916%0.099%KU232289.1Select seq gb|KU232299.1|Zika virus isolate 015ZV_PEBR15 NS5 protein gene, partial cds118711876%0.099%KU232299.1Select seq gb|KU232291.1|Zika virus isolate 051ZV_PEBR15 NS5 protein gene, partial cds118611866%0.099%KU232291.1Select seq gb|KU758878.1|Zika virus polyprotein gene, partial cds113311336%0.099%KU758878.1Select seq gb|KF270886.1|Zika virus strain CCB-870 envelope glycoprotein gene, partial cds107710778%0.089%KF270886.1Select seq gb|AF372422.1|AF372422Zika virus envelope protein (E) gene, partial cds102010208%0.087%AF372422.1Select seq gb|KU867812.1|Zika virus isolate Jiangxi.CHN/01/2016 nonstructural protein 5 gene, partial cds101810185%0.0100%KU867812.1Select seq gb|KF270887.1|Zika virus strain CCB-870 NS3 protein gene, partial cds9969967%0.089%KF270887.1Select seq gb|KF383022.1|Zika virus strain ArD127988 envelope protein gene, partial cds9809807%0.089%KF383022.1Select seq gb|EF158064.1|St. Louis encephalitis virus strain GML 902612 polyprotein gene, partial cds980198664%0.070%EF158064.1Select seq gb|KF383026.1|Zika virus strain ArD127994 envelope protein gene, partial cds9759757%0.089%KF383026.1Select seq gb|EU303241.1|Zika virus note MR766 (p4 15/9/76) nonstructural protein 5 (NS5) gene, partial cds9679677%0.088%EU303241.1Select seq gb|KF383032.1|Zika virus strain ArD30101 envelope protein gene, partial cds9669667%0.088%KF383032.1Select seq gb|KF383034.1|Zika virus strain ArD30156 envelope protein gene, partial cds9629627%0.088%KF383034.1Select seq gb|KF383029.1|Zika virus strain ArD165522 envelope protein gene, partial cds9629627%0.088%KF383029.1Select seq gb|KF383037.1|Zika virus strain ArA506 envelope protein gene, partial cds9609607%0.088%KF383037.1Select seq gb|KF383036.1|Zika virus strain ArA975 envelope protein gene, partial cds9579577%0.088%KF383036.1Select seq gb|KF383033.1|Zika virus strain AnD30332 envelope protein gene, partial cds9579577%0.088%KF383033.1Select seq gb|KF383039.1|Zika virus strain HD78788 envelope protein gene, partial cds9399397%0.088%KF383039.1Select seq gb|KF383028.1|Zika virus strain ArD165531 envelope protein gene, partial cds9399397%0.088%KF383028.1Select seq gb|KF383031.1|Zika virus strain ArD9957 envelope protein gene, partial cds9359357%0.088%KF383031.1Select seq gb|KF383038.1|Zika virus strain ArA986 envelope protein gene, partial cds9309307%0.087%KF383038.1Select seq gb|KF383046.1|Zika virus strain ArA982 envelope protein gene, partial cds9219217%0.087%KF383046.1Select seq gb|EU566860.1|St. Louis encephalitis virus strain Hubbard, complete genome915204758%0.069%EU566860.1Select seq gb|KT823415.1|St. Louis encephalitis virus strain RT 121B polyprotein gene, complete cds913205064%0.069%KT823415.1Select seq gb|FJ753286.2|St. Louis encephalitis virus strain CbaAr-4005, complete genome913202564%0.069%FJ753286.2Select seq gb|KF383045.1|Zika virus strain ArA27106 envelope protein gene, partial cds9069067%0.087%KF383045.1Select seq gb|KF383043.1|Zika virus strain ArA27443 envelope protein gene, partial cds9029027%0.087%KF383043.1Select seq gb|KF383035.1|Zika virus strain MR1429 envelope protein gene, partial cds9019017%0.086%KF383035.1Select seq gb|KF383103.1|Zika virus strain ArA986 nonstructural protein 5 gene, partial cds8978976%0.088%KF383103.1Select seq gb|KF383086.1|Zika virus strain ArA975 nonstructural protein 5 gene, partial cds8978976%0.088%KF383086.1Select seq gb|KF383041.1|Zika virus strain ArA27290 envelope protein gene, partial cds8978977%0.087%KF383041.1Select seq gb|KF383042.1|Zika virus strain ArA27096 envelope protein gene, partial cds8938937%0.086%KF383042.1Select seq gb|EF158069.1|St. Louis encephalitis virus strain 72 V 4749 polyprotein gene, partial cds890196267%0.069%EF158069.1Select seq gb|KF383040.1|Zika virus strain ArA27101 envelope protein gene, partial cds8668667%0.086%KF383040.1Select seq gb|KF383104.1|Zika virus strain ArA982 nonstructural protein 5 gene, partial cds8658656%0.087%KF383104.1Select seq gb|KF383085.1|Zika virus strain ArD9957 nonstructural protein 5 gene, partial cds8658656%0.087%KF383085.1Select seq gb|KF383106.1|Zika virus strain ArA27443 nonstructural protein 5 gene, partial cds8618616%0.087%KF383106.1Select seq gb|KF383114.1|Zika virus strain AnD30332 nonstructural protein 5 gene, partial cds8568566%0.087%KF383114.1Select seq gb|KF383107.1|Zika virus strain ArA27407 nonstructural protein 5 gene, partial cds8568566%0.087%KF383107.1Select seq gb|KF383088.1|Zika virus strain ArD30101 nonstructural protein 5 gene, partial cds8568566%0.087%KF383088.1Select seq gb|KF383087.1|Zika virus strain ArD30156 nonstructural protein 5 gene, partial cds8528526%0.087%KF383087.1Select seq gb|KF383089.1|Zika virus strain ArD165531 nonstructural protein 5 gene, partial cds8388386%0.086%KF383089.1Select seq dbj|AB908162.1|Zika virus gene for polyprotein, partial cds, strain: ZIKV Hu/Tahiti/01u/2014NIID8388384%0.099%AB908162.1Select seq gb|KF383101.1|Zika virus strain ArD127710 nonstructural protein 5 gene, partial cds8348346%0.086%KF383101.1Select seq gb|KF383097.1|Zika virus strain ArD127994 nonstructural protein 5 gene, partial cds8348346%0.086%KF383097.1Select seq gb|KF383099.1|Zika virus strain ArD127987 nonstructural protein 5 gene, partial cds8298296%0.086%KF383099.1Select seq gb|KF383098.1|Zika virus strain ArD127988 nonstructural protein 5 gene, partial cds8258256%0.086%KF383098.1Select seq gb|KU872850.1|Zika virus isolate Dominican Rep-Rus-2016, NS2 partial cds8148144%0.099%KU872850.1

LOCUS KU509998 10676 bp RNA linear VRL 02-FEB-2016 DEFINITION Zika virus strain Haiti/1225/2014, complete genome. ACCESSION KU509998 VERSION KU509998.1 GI:983657312 KEYWORDS . SOURCE Zika virus ORGANISM Zika virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus. REFERENCE 1 (bases 1 to 10676) AUTHORS Lednicky,J.A., Morris,J.G. Jr., Beau De Rochars,V.M., Elbadry,M.A., Okech,B.A. and Loeb,J.C. TITLE Zika virus from a Haitian, December 2014 JOURNAL Unpublished REFERENCE 2 (bases 1 to 10676) AUTHORS Lednicky,J.A., Morris,J.G. Jr., Beau De Rochars,V.M., Elbadry,M.A., Okech,B.A. and Loeb,J.C. TITLE Direct Submission JOURNAL Submitted (10-JAN-2016) Environmental and Global Health, University of Florida - Gainesville, 1225 Center Drive, Room 4155, Gainesville, FL 32610, USA COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10676 /organism="Zika virus" /mol_type="genomic RNA" /strain="Haiti/1225/2014" /isolation_source="plasma" /host="Homo sapiens" /db_xref="taxon:64320" /country="Haiti" /collection_date="12-Dec-2014" /note="genotype: Asian"http://www.ncbi.nlm.nih.gov/nuccore/KU509998.1

Science paper http://science.sciencemag.org/content/early/2016/03/23/science.aaf5036.full.pdf+html

The recent Science paper of sequences in the Americas including those in Brazil noted that a timeline based on the 20 sequences in Figure 3 suggested that Zika arrived in the Americas in 2013. The early cases as well as the number of cases in Brazil suggested that this early entry was in Brazil. However, the earliest sequence reported for the Americas (excluding those from Chile owned Easter Islands) was a Dec 12, 2014 collect from Haiti, 3 months prior to the earliest sequence from Brazil (Mar 14, 2015 for the 20 sequences in Fig 3). The sequences in the Americas are closely related to those from the 2013 outbreak in French Polynesia. Although sequences were generated for residents in French Polynesia, including a sequence from Marquesas Islands, almost 1000 miles northeast of Tahiti, these sequences were short (in the range of 200-400 BP and were excluded from fig 3, which used sequences greater than 1500 BP). Similarly, most of the sequences from French Polynesia were from travelers from New Caledonia, Japan, Norway, and France, which were also short. The only full sequence, H/PF/2013, was from a 51 year old female diagnosed in metropolitan France (labeled as KJ776791 French Polynesia in fig 3). See sequence map https://www.google.com/maps/d/u/0/edit?mid=zv94AJqgUct4.kI8kcFySb4J0&hl=en The 20 sequences from the Americas are most closely related to this sequence. However, several of the sequences from Suriname are included, and the shorter sequences are between 1900-2600 BP, indicating that the “B” branch (the sequences from the Americas) is defined by a single nucleotide change (T2547C of the coding sequence). Sequences covering this region are limited. None of the shorter French Polynesia sequences cover position 2544, which is also true of subsequent sequences from Zika outbreaks in New Caledonia and Easter Island. There were a limited number of short sequences associated with local transmission in New Caledonia, but there are 51 sequences from Easter Island (2014 collections). However, these sequences are all under 1000 BP and cover the NS5 region at the 3’ end of the 10,272 BP protein coding region, which also does not overlap position 2547. Thus, the only 2013/2014 sequence from French Polynesia, New Caledonia, or Easter Island covering position 2544 is the H/PF/2013 sequence. Therefore, the possibility that T2547C was present on these sequences, collected prior to sequences from the Americas, cannot be excluded, so the earlier evolution could have been in these countries, or any other country in the South Pacific or the Americas. In fact, the earliest sequence from the Americas is from Haiti (labeled as KU509998 Haiti) in Fig 3. It is the sequence with the earliest collection date (Dec 12, 2014) and is a precursor for the earliest sequence from Brazil (labeled KU321639 Brazil) which was collected on Mar 14, 2015 and is on the same branch as the Haiti sequence. Thus, more information on this case (local or travel from some other country), would aid in determining the location of the first “B” group sequence in the Americas, as would full sequences from 2013/2014 collections from French Polynesia, New Caledonia, or Eastern Island.

Colombia is investigating 41 cases of microcephaly for connection with zikaA A + A ++The National Institute of Health (INS) of Colombia is investigating whether 41 cases of microcephaly in newborns related to Zika virus, which so far has infected a total of 58,838 people. According to the latest bulletin of the INS until the eleventh week of the year 50 cases of microcephaly are recorded in newborns, nine of which have been negative for zika. The Institute, which warned that the number of cases may vary "because of the late notification of the event", said that compared with the historical monthly average of microcephaly in the country "a cumulative total of 30 cases at the same time be expected". With regard to Guillain Barré, polyneuropathy and other complications associated with zika syndrome, have been reported to the surveillance system 381 cases "with a history of febrile illness consistent with infection" by the virus. According to estimates by the Colombian Government, 500 babies may be born with microcephaly in the country by zika and a similar number of people may experience Guillain Barre syndrome, before suggesting a resolution to couples "not pregnant" during the expansion phase virus. With respect to the total number infected, the INS updated the figure to 58,838 people, of whom 10,812 are pregnant women. Virus Zika, transmitted by a mosquito of the genus Aedes, the same one that causes dengue and chikungunya, can cause fever, red eyes without discharge without itching, rash with white or red spots and less frequently muscle and joint pain . Zika virus cases down in Cucuta, Colombia, but increases in other municipalities The municipality of Cucuta in Colombia is one of the most affected by Zika virus in the region, and has the top spot for the greatest number of patients. However, the National Institute of Health (INS) said that in Colombia zika epidemic is declining and that municipality weekly incidence of cases has decreased. http://www.buhola.com/noticias/56f6d0514ed9e4cc7df1d144

Monday, March 28, 2016 - 08:10Colombia checks relationship between Zika virus and 41 cases of babies with microcephalyPhoto: Getty ImagesThe National Institute of Health (NIH) of Colombia is in the process of analyzing 41 cases of microcephaly registered in the country. Researchers aim to find out if there is a relationship between the malformation and the Zika virus, which has infected 58,838 people, according to the latest INS newsletter. They were reported in total 50 cases of microcephaly in newborns, but nine were negative for Zika. The agency also warned, according to the Earth website, the number may vary "because of the late notification." were also recorded 381 cases of Guillain-Barré syndrome and other complications related to Zika, "with febrile disease history compatible with infection. Colombia estimates that may be born 500 babies with microcephaly caused by Zika. Authorities suggest that women avoid getting pregnant during the spread of the virus.http://www.bahianoticias.com.br/saude/noticia/16657-colombia-verifica-relacao-entre-zika-virus-e-41-casos-de-bebes-com-microcefalia.html?utm_source=dlvr.it&utm_medium=twitter

In Antioquia six cases of microcephaly associated with zika studiedANTIOQUIALOCALMarch 27, 2016 - 2:16 Pm - Tags: Antioquia , National Institute Of Health , Microcephaly , Zika INS reported 42,706 cases in the country zika / AFP Photo According to the latest epidemiological report by the National Institute of Health, in Antioquia are six cases of microcephaly, apparently associated with the Zika virus. Health officials in the department confirmed that three cases are reported in Medellin, two in Urrao and one in Guarne. Public health manager in Antioquia, Castrillón Ricardo Quintero, said it is premature to say that cases of microcephaly are related to Zika virus.However, he clarified that mothers and their babies were conducted laboratory tests and are awaiting the results. Castrillón Quintero recalled that the municipalities of Turbo and Apartadó in Urabá and Caucasia in Bajo Cauca, are the most likely areas of contracting the virus Zika and stressed that the department has slowed by 30 percent endemic process the illness. According to the National Institute of Health, in total, Colombia reported 58,000 cases of zika 838 since the start of the circulation of the virus. In Antioquia, so far, are 99 confirmed cases of the Zika virus.

According to the latest epidemiological report by the National Institute of Health, in Antioquia are six cases of microcephaly, apparently associated with the Zika virus. Health officials in the department confirmed that three cases are reported in Medellin, two in Urrao and one in Guarne. http://www.rcnradio.com/locales/antioquia-se-estudian-seis-casos-microcefalia-asociados-zika/?utm_source=dlvr.it&utm_medium=twitter

Neurologists Colombians began Saturday a survey to identify the consequences of zika virus in the human nervous system through a new network of study and research established at the University of the Valley in the city of Cali. KNOW MOREResearch investigates possible neurological problems caused by zika in adults Honduras records 40 cases of syndrome possibly associated with the virus zika What is true and what is rumor about the zika The formation of this group of experts who work in Colombia and the United States, was announced during the XII Colombian Congress of Neurology, held in the last 25 and 26. Ali, the director of the research center in myelitis Johns Hopkins University, Carlos Pardo Villamizar, and the dean of Health Sciences Faculty of the University Icesi of Cali, Yuri Takeuchi, gave details about the project. "It's a collaborative study centered at the University of the Valley, where we conducted laboratory tests to establish the diagnosis of zika, they also joined Cúcuta universities, Santander, Antioquia, Barranquilla and Bogota, and neurologists colleagues interested in the research" he explained Pardo Efe. Takeuchi pointed out that the study is focused on the consequences in adults who were related to the virus zika such as encephalitis, Guillain-Barré syndrome and myelitis, but there is also a group interested in the effects of the disease in pregnant women and their children . The group, which was dubbed Study Neurovírus Emerging in the Americas (NEAS), began to collect data and analyze the most significant cases of zika in the country, among the 42,706 already confirmed by the National Institute of Health (NIH) of Colombia. According to Pardo, yet the research group came to the hypothesis that the virus "may be causing an immune reaction that produces inflammatory diseases of the central nervous system or peripheral nervous system." In addition, Brown said the NEAS also considering the zika "may be attacking directly or areas of the nervous system structures such as peripheral nerves, but it's still a part of the research." However, the director of the research center in myelitis from Johns Hopkins said that if confirmed the direct relationship between this virus and neurological diseases, "the aggressiveness of zika is much greater than that of dengue and may be greater than that of chikungunya ". "One of the observations is that the problems we assume that are associated with infection zika can produce more often than neurological dengue problems," he told Efe. The virus zika is transmitted by the mosquito Aedes aegypti, the same vector of chikungunya and dengue. The zika is currently present in much of Latin America and the Caribbean and, despite causing mild symptoms in most infected, concerned especially for his probable relationship to cases of microcephaly in newborns and neurological syndrome Guillain -Barre. http://saude.terra.com.br/medicos-colombianos-iniciam-pesquisa-sobre-sequelas-neurologicas-do-zika,91a4a93af97d15444cf5501b5571efe4i5wju6to.html?utm_source=twitterfeed&utm_medium=twitter

Colombian doctors begin research on neurological sequelae of zikahttp://saude.terra.com.br/medicos-colombianos-iniciam-pesquisa-sobre-sequelas-neurologicas-do-zika,91a4a93af97d15444cf5501b5571efe4i5wju6to.html?utm_source=twitterfeed&utm_medium=twitter FEB 272016 12h47

Pregnant, eighth case of Zika in CubaA Cuban doctor from Brazil tested positive for evidence of the disease. The authorities of the island will begin on Monday the second campaign against the Aedes Aegypti mosquito, which carries the virus. PrintCommentshare: Spraying against mosquitoes that carry Zika in Venezuela inSharemenéameRelated PostsUS warns of risk in Cuba ZikaRecommend pregnant women not to travel to Cuba and the rest of the population to protect yourself from mosquito bites. Authorities issued an alert level 2 out of 3.Reported another case of Zika in CubaThere are now six cases of Zika in Cuba; five of them imported and one native.Cuba recorded its first indigenous case of the Zika virusA young Havana who has not traveled abroad was diagnosed with the virus after symptoms of the disease, an official note from the Ministry of Public Health.Zika fourth case in Cuba, all via VenezuelaThe Ministry of Public Health reported Wednesday that a Cuban computer engineer from Caracas tested positive for the virus. Martinoticias.comMarch 27, 2016 Tras to publicize the eighth case of Zika in Cuba , in a briefing note published Saturday, the Ministry of Public Health and the authorities of the island will begin on Monday the second campaign against the Aedes Aegypti mosquito, transmitter of the disease. Although the Cuban authorities claim that the spread of the disease has been controlled through the first campaign launched against the transmitter vector, which mobilized more than 9,000 membersof the armed forces and police, a new case of Zika has been confirmed this week , that of a pregnant . "Diagnosed the seventh imported case of patient confirmed with Zika virus, corresponding to Cuban partner, MGI specialist, 36 years old, who arrived in the country from Brazil on March 22, resident in street B No. 304 between Calixto Garcia and Los Maceos city of Guantanamo , "explained an information note MINSAP Saturday. The patient had not shown symptoms of the virus, but was admitted immediately because it is a pregnant 28 weeks gestation, authorities said. A test sent to the Institute of Tropical Medicine Pedro Kouri was positive. In the second season , the island will deploy thousands of students across the country to educate people on the importance of eradicating the mosquito, as well as new spraying equipment, as made known local media. In a report on the results of the previous season, Cuban health authorities also said the municipalities with the highest incidence of dengue on the island. " It is reported dengue transmission in 14 provinces and 46 municipalities , and decreased the incidence rate of suspected cases of this virus is maintained in this week regarding the above. The highest indicators are reported in the provinces of Santiago de Cuba, Ciego de Avila Guantanamo, Artemisa, Mayabeque and Havana , "the report said . http://www.martinoticias.com/content/embarazada-octavo-caso-zika-cuba/118305.html?utm_source=twitterfeed&utm_medium=twitter

Cuba announces seventh event in the country ZikaIn this article: Cuba , ZikaMarch 27, 2016 | + | Health authorities in Cuba announced comienzode a second stage in the fight against the mosquito, after announcing the seventh case of Zika detected in the country The Ministry of Public Health of Cuba confirmed Sunday that a pregnant woman, identified as a Cuban medical, sick of the dangerous virus last week after returning from Brazil, one of the countries most affected area. Cuba strives to "hold off" not only cases of zika, but also other expanding tropical virus such as dengue and chikungunya. http://www.cubadebate.cu/noticias/2016/03/27/cuba-anuncia-septimo-caso-de-zika-en-el-pais/#.Vvf7cuIrJgI