niman

Align Segment-ID Name Score E-Value Identity EPI860509 A/tufted duck/Germany-SH/R8444/2016 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI860393 A/tufted_duck/Germany/AR8444-L01987/2016 (A/H5N8) segment 4 (HA) 3142.3 0.000000e+00 1703/1704 (99%) EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI861011 A/turkey/Germany-SH/R8595/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3131.2 0.000000e+00 1701/1704 (99%) EPI860401 A/tufted_duck/Germany/AR8459-L01988/2016 (A/H5N8) segment 4 (HA) 3125.7 0.000000e+00 1700/1704 (99%) EPI859653 A/tufted_duck/Germany/AR8444-L01986/2016 (A/H5N8) segment 4 (HA) 3125.7 0.000000e+00 1700/1704 (99%) EPI863865 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI861224 A/chicken/Germany-SH/R8758/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI861012 A/chicken/Germany-MV/R8790/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI860231 A/wild duck/Poland/82A/2016 (A/H5N8) segment 4 (HA) 3110.9 0.000000e+00 1697/1704 (99%) EPI861222 A/tufted duck/Germany-SN/R8795/2016 (A/H5N8) segment 4 (HA) 3107.2 0.000000e+00 1695/1704 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3105.3 0.000000e+00 1696/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863857 A/Chicken/Sweden/SVA161122KU0453/SZ0209321/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863849 A/Chicken/Sweden/SVA161122KU0453/SZ0209318/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863834 A/Chicken/Sweden/SVA161122KU0453/SZ0209317/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863826 A/Chicken/Sweden/SVA161122KU0453/SZ0209316/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3099.8 0.000000e+00 1695/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI869929 A/mute swan/Poland/108/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI861572 A/mute swan/Croatia/78/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI873624 A/mute swan/Croatia/9/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI869809 A/duck/France/161108h/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI869926 A/domestic goose/Poland/72/2016 (A/H5N8) segment 4 (HA) 3072.1 0.000000e+00 1690/1704 (99%) EPI836614 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI836606 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI869927 A/chicken/Poland/79A/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI858836 A/duck/India/10CA01/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774394 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774386 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774310 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774242 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774234 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774121 A/Bar-headed Goose/Qinghai/BTY1-LV/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774113 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI869928 A/chicken/Poland/85A/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI858844 A/painted stork/India/10CA03/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774475 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774458 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774450 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774402 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774378 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774326 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774318 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774302 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774277 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774267 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774259 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774251 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774226 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774218 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774210 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774201 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774193 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774185 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774176 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774168 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774159 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774150 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774506 A/Great Black-headed Gull/Qinghai/YO1-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774490 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774483 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774434 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774410 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774369 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774335 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774294 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774286 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774142 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774133 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774466 A/Brown-headed Gull/Qinghai/ZTO5-B/2016 (A/H5N8) segment 4 (HA) 3042.6 0.000000e+00 1685/1704 (98%) EPI774442 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 4 (HA) 3042.6 0.000000e+00 1686/1705 (98%) EPI774352 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 4 (HA) 3042.6 0.000000e+00 1685/1704 (98%) EPI873212 A/chicken/Croatia/103/2016 (A/H5N8) segment 4 (HA) 3038.9 0.000000e+00 1683/1702 (98%) EPI774498 A/Brown-headed Gull/Qinghai/ZTO6-MU/2016 (A/H5N8) segment 4 (HA) 3037.0 0.000000e+00 1684/1704 (98%) EPI774426 A/Brown-headed Gull/Qinghai/ZTO1-B/2016 (A/H5N8) segment 4 (HA) 3037.0 0.000000e+00 1684/1704 (98%) EPI774361 A/Bar-headed Goose/Qinghai/BTY15-LU/2016 (A/H5N8) segment 4 (HA) 3037.0 0.000000e+00 1684/1704 (98%) EPI774344 A/Bar-headed Goose/Qinghai/BTY14-LU/2016 (A/H5N8) segment 4 (HA) 3031.5 0.000000e+00 1683/1704 (98%) EPI703608 A/duck/Eastern China/S1109/2014 (A/H5N8) segment 4 (HA) 3031.5 0.000000e+00 1683/1704 (98%) EPI681300 A/goose/Zhejiang/925104/2014 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1671/1704 (98%) EPI681299 A/goose/Zhejiang/925037/2014 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1671/1704 (98%) EPI860239 A/tufted duck/Denmark/17740-1/2016 (A/H5N8) segment 4 (HA) 2955.8 0.000000e+00 1606/1609 (99%) EPI703602 A/duck/Eastern China/S0215/2014 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1669/1704 (97%) EPI675774 A/duck/Liaoning/S1001/2014 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1669/1704 (97%) EPI681290 A/goose/Zhejiang/925106/2014 (A/H5N6) segment 4 (HA) 2942.8 0.000000e+00 1667/1704 (97%) EPI509698 A/breeder duck/Korea/Gochang1/2014 (A/H5N8) segment 4 (HA) 2937.3 0.000000e+00 1666/1704 (97%) EPI690792 A/goose/Jiangsu/QD5/2014 (A/H5N8) segment 4 (HA) 2926.2 0.000000e+00 1664/1704 (97%) EPI561495 A/Baikal teal/Korea/H52/2014 (A/H5N8) segment 4 (HA) 2926.2 0.000000e+00 1664/1704 (97%) EPI591879 A/duck/Jiangxi/23970/2013 (A/H0) segment 4 (HA) 2920.7 0.000000e+00 1663/1704 (97%) EPI703599 A/duck/Eastern China/S1210/2013 (A/H5N8) segment 4 (HA) 2915.1 0.000000e+00 1662/1704 (97%) EPI762158 A/goose/Eastern China/CZ/2013 (A/H5N8) segment 4 (HA) 2909.6 0.000000e+00 1662/1705 (97%)

Align Segment-ID Name Score E-Value Identity EPI859649 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 6 (NA) 2593.8 0.000000e+00 1405/1406 (99%) EPI863867 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 6 (NA) 2592.0 0.000000e+00 1405/1406 (99%) EPI859213 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 6 (NA) 2580.9 0.000000e+00 1403/1406 (99%) EPI860232 A/wild duck/Poland/82A/2016 (A/H5N8) segment 6 (NA) 2575.3 0.000000e+00 1402/1406 (99%) EPI863851 A/Chicken/Sweden/SVA161122KU0453/SZ0209318/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI863836 A/Chicken/Sweden/SVA161122KU0453/SZ0209317/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI863828 A/Chicken/Sweden/SVA161122KU0453/SZ0209316/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI863859 A/Chicken/Sweden/SVA161122KU0453/SZ0209321/2016 (A/H5N8) segment 6 (NA) 2566.1 0.000000e+00 1400/1406 (99%) EPI869689 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 6 (NA) 2564.3 0.000000e+00 1400/1406 (99%) EPI861569 A/mute swan/Croatia/70/2016 (A/H5N8) segment 6 (NA) 2558.7 0.000000e+00 1399/1406 (99%) EPI869935 A/mute swan/Poland/108/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI869933 A/chicken/Poland/79A/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI869811 A/duck/France/161108h/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI868850 A/turkey/England/052131/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI864747 A/mute swan/Croatia/85/2016 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI861573 A/mute swan/Croatia/78/2016 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI873213 A/chicken/Croatia/103/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI869934 A/chicken/Poland/85A/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774500 A/Brown-headed Gull/Qinghai/ZTO6-MU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774468 A/Brown-headed Gull/Qinghai/ZTO5-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774452 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774444 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774436 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774420 A/Bar-headed Goose/Qinghai/BTY18-MU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774412 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774404 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774388 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774380 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774371 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774363 A/Bar-headed Goose/Qinghai/BTY15-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774346 A/Bar-headed Goose/Qinghai/BTY14-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774337 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774320 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774312 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774304 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774270 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774261 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774253 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774244 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774195 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774187 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774144 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774136 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI774115 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI873627 A/mute swan/Croatia/104/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI858846 A/painted stork/India/10CA03/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI836616 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI836608 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI823758 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI823750 A/wild duck/Tyva/35/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI823462 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774492 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774484 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774477 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1396/1407 (99%) EPI774460 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1396/1407 (99%) EPI774396 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1393/1403 (99%) EPI774354 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774328 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774296 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774279 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1393/1403 (99%) EPI774178 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774170 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774161 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI873625 A/mute swan/Croatia/9/2017 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774508 A/Great Black-headed Gull/Qinghai/YO1-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774428 A/Brown-headed Gull/Qinghai/ZTO1-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774288 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774220 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774212 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1392/1403 (99%) EPI774152 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1395/1407 (99%) EPI773759 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI869932 A/domestic goose/Poland/72/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI869925 A/domestic goose/Poland/33/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774236 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1394/1407 (99%) EPI869941 A/herring gull/Poland/84/2016 (A/H5N8) segment 6 (NA) 2523.6 0.000000e+00 1368/1369 (99%) EPI774228 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1393/1407 (99%) EPI858838 A/duck/India/10CA01/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1391/1406 (98%) EPI774203 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1391/1406 (98%) EPI703648 A/duck/Eastern China/S1109/2014 (A/H5N8) segment 6 (NA) 2492.3 0.000000e+00 1388/1407 (98%) EPI703642 A/duck/Eastern China/S0215/2014 (A/H5N8) segment 6 (NA) 2464.6 0.000000e+00 1383/1407 (98%) EPI681357 A/goose/Zhejiang/925037/2014 (A/H5N8) segment 6 (NA) 2447.9 0.000000e+00 1380/1407 (98%) EPI681358 A/goose/Zhejiang/925104/2014 (A/H5N8) segment 6 (NA) 2425.8 0.000000e+00 1376/1407 (97%) EPI583873 A/duck/Shaoxing/5240/2013 (A/H0) segment 6 (NA) 2414.7 0.000000e+00 1374/1407 (97%) EPI591884 A/duck/Jiangxi/23970/2013 (A/H0) segment 6 (NA) 2409.2 0.000000e+00 1373/1407 (97%) EPI690794 A/goose/Jiangsu/QD5/2014 (A/H5N8) segment 6 (NA) 2403.6 0.000000e+00 1372/1407 (97%) EPI561493 A/Baikal teal/Korea/H52/2014 (A/H5N8) segment 6 (NA) 2403.6 0.000000e+00 1372/1407 (97%) EPI530990 A/duck/Zhejiang/6D18/2013 (A/H5N8) segment 6 (NA) 2403.6 0.000000e+00 1373/1408 (97%) EPI509700 A/breeder duck/Korea/Gochang1/2014 (A/H5N8) segment 6 (NA) 2403.6 0.000000e+00 1372/1407 (97%) EPI762160 A/goose/Eastern China/CZ/2013 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1371/1407 (97%) EPI703638 A/duck/Eastern China/S1210/2013 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1371/1407 (97%) EPI530989 A/duck/Zhejiang/W24/2013 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1372/1408 (97%) EPI681355 A/duck/Zhejiang/925019/2014 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1370/1407 (97%) EPI762152 A/duck/Eastern China/JY/2014 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1369/1407 (97%) EPI703635 A/duck/Eastern China/L1021/2012 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1369/1407 (97%) EPI690802 A/goose/Shandong/WFSG1/2014 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1369/1407 (97%) EPI681356 A/duck/Zhejiang/925169/2014 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1370/1408 (97%) EPI703633 A/duck/Eastern China/L0611/2011 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1368/1407 (97%) EPI646030 A/goose/Jiangsu/WX202/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1368/1407 (97%) EPI507675 A/mallard duck/Shanghai/SH-9/2013 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1369/1408 (97%) EPI602039 A/duck/Ningbo/3262/2013 (A/H0) segment 6 (NA) 2379.6 0.000000e+00 1349/1379 (97%)

Align Segment-ID Name Score E-Value Identity EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3131.2 0.000000e+00 1701/1704 (99%) EPI863865 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI860231 A/wild duck/Poland/82A/2016 (A/H5N8) segment 4 (HA) 3110.9 0.000000e+00 1697/1704 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3105.3 0.000000e+00 1696/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863857 A/Chicken/Sweden/SVA161122KU0453/SZ0209321/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863849 A/Chicken/Sweden/SVA161122KU0453/SZ0209318/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863834 A/Chicken/Sweden/SVA161122KU0453/SZ0209317/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI863826 A/Chicken/Sweden/SVA161122KU0453/SZ0209316/2016 (A/H5N8) segment 4 (HA) 3103.5 0.000000e+00 1696/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3099.8 0.000000e+00 1695/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3092.4 0.000000e+00 1694/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 3083.2 0.000000e+00 1692/1704 (99%) EPI869929 A/mute swan/Poland/108/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI861572 A/mute swan/Croatia/78/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI873624 A/mute swan/Croatia/9/2017 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI869809 A/duck/France/161108h/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI869926 A/domestic goose/Poland/72/2016 (A/H5N8) segment 4 (HA) 3072.1 0.000000e+00 1690/1704 (99%) EPI836614 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI836606 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI869927 A/chicken/Poland/79A/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI858836 A/duck/India/10CA01/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774394 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774386 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774310 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774242 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774234 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774121 A/Bar-headed Goose/Qinghai/BTY1-LV/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI774113 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI869928 A/chicken/Poland/85A/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI858844 A/painted stork/India/10CA03/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774475 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774458 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774450 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774402 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774378 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774326 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774318 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774302 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774277 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774267 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774259 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774251 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774226 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774218 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774210 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774201 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774193 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774185 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774176 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774168 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774159 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774150 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI774506 A/Great Black-headed Gull/Qinghai/YO1-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774490 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774483 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774434 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774410 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774369 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774335 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774294 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774286 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774142 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774133 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI774466 A/Brown-headed Gull/Qinghai/ZTO5-B/2016 (A/H5N8) segment 4 (HA) 3042.6 0.000000e+00 1685/1704 (98%) EPI774442 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 4 (HA) 3042.6 0.000000e+00 1686/1705 (98%) EPI774352 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 4 (HA) 3042.6 0.000000e+00 1685/1704 (98%) EPI873212 A/chicken/Croatia/103/2016 (A/H5N8) segment 4 (HA) 3038.9 0.000000e+00 1683/1702 (98%) EPI774498 A/Brown-headed Gull/Qinghai/ZTO6-MU/2016 (A/H5N8) segment 4 (HA) 3037.0 0.000000e+00 1684/1704 (98%) EPI774426 A/Brown-headed Gull/Qinghai/ZTO1-B/2016 (A/H5N8) segment 4 (HA) 3037.0 0.000000e+00 1684/1704 (98%) EPI774361 A/Bar-headed Goose/Qinghai/BTY15-LU/2016 (A/H5N8) segment 4 (HA) 3037.0 0.000000e+00 1684/1704 (98%) EPI774344 A/Bar-headed Goose/Qinghai/BTY14-LU/2016 (A/H5N8) segment 4 (HA) 3031.5 0.000000e+00 1683/1704 (98%) EPI703608 A/duck/Eastern China/S1109/2014 (A/H5N8) segment 4 (HA) 3031.5 0.000000e+00 1683/1704 (98%) EPI681300 A/goose/Zhejiang/925104/2014 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1671/1704 (98%) EPI681299 A/goose/Zhejiang/925037/2014 (A/H5N8) segment 4 (HA) 2965.0 0.000000e+00 1671/1704 (98%) EPI860239 A/tufted duck/Denmark/17740-1/2016 (A/H5N8) segment 4 (HA) 2955.8 0.000000e+00 1606/1609 (99%) EPI703602 A/duck/Eastern China/S0215/2014 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1669/1704 (97%) EPI675774 A/duck/Liaoning/S1001/2014 (A/H5N8) segment 4 (HA) 2953.9 0.000000e+00 1669/1704 (97%) EPI681290 A/goose/Zhejiang/925106/2014 (A/H5N6) segment 4 (HA) 2942.8 0.000000e+00 1667/1704 (97%) EPI509698 A/breeder duck/Korea/Gochang1/2014 (A/H5N8) segment 4 (HA) 2937.3 0.000000e+00 1666/1704 (97%) EPI690792 A/goose/Jiangsu/QD5/2014 (A/H5N8) segment 4 (HA) 2926.2 0.000000e+00 1664/1704 (97%) EPI561495 A/Baikal teal/Korea/H52/2014 (A/H5N8) segment 4 (HA) 2926.2 0.000000e+00 1664/1704 (97%) EPI591879 A/duck/Jiangxi/23970/2013 (A/H0) segment 4 (HA) 2920.7 0.000000e+00 1663/1704 (97%) EPI703599 A/duck/Eastern China/S1210/2013 (A/H5N8) segment 4 (HA) 2915.1 0.000000e+00 1662/1704 (97%) EPI762158 A/goose/Eastern China/CZ/2013 (A/H5N8) segment 4 (HA) 2909.6 0.000000e+00 1662/1705 (97%) EPI703607 A/goose/Eastern China/S0408/2014 (A/H5N8) segment 4 (HA) 2909.6 0.000000e+00 1661/1704 (97%) EPI690808 A/goose/Yangzhou/0420/2014 (A/H5N8) segment 4 (HA) 2909.6 0.000000e+00 1662/1705 (97%) EPI690800 A/goose/Shandong/WFSG1/2014 (A/H5N8) segment 4 (HA) 2909.6 0.000000e+00 1661/1704 (97%) EPI602037 A/duck/Ningbo/3262/2013 (A/H0) segment 4 (HA) 2904.1 0.000000e+00 1660/1704 (97%) EPI583870 A/duck/Shaoxing/5240/2013 (A/H0) segment 4 (HA) 2904.1 0.000000e+00 1660/1704 (97%) EPI530985 A/duck/Zhejiang/W24/2013 (A/H5N8) segment 4 (HA) 2904.1 0.000000e+00 1660/1704 (97%) EPI762150 A/duck/Eastern China/JY/2014 (A/H5N8) segment 4 (HA) 2898.5 0.000000e+00 1660/1705 (97%) EPI530986 A/duck/Zhejiang/6D18/2013 (A/H5N8) segment 4 (HA) 2898.5 0.000000e+00 1659/1704 (97%)

Isolate name: A/tufted duck/Germany-SH/R8444/2016 Isolate ID: EPI_ISL_237958 Passage details/history: swab Type: A / H5N8 Lineage: Sample information Collection date: 2016-11 (Day unknown) Host Aythya fuligula Additional host information: Domestic status: Wild Is vaccinated: N Location: Germany / Schleswig-Holstein Additional location information: Health status: Dead Specimen source: Strain or commercial product name used for vaccination: Institute information Originating lab: Sample ID given by the sample provider: Address: Submitting lab: Friedrich-Loeffler-Institut Sample ID given by the submitting laboratory: AR8444/16 Authors: Address: Friedrich-Loeffler-Institut Suedufer 10 17493 Greifswald-Insel Riems Germany Publication Publication In vivo antiviral resistance Phenotype Genotype Unspecified Antiviral resistance tested by experimental procedures Adamantanes: Unknown Oseltamivir: Unknown Zanamivir: Unknown Peramivir: Unknown Other: Unknown Additional information Antigenic characterization: Note: Sequence segment identifier length accession # INSDC Sequence PB2 A/tufted duck/Germany-SH/R8444/2016 2306 EPI860494 PB1 A/tufted duck/Germany-SH/R8444/2016 2294 EPI860495 PA A/tufted duck/Germany-SH/R8444/2016 1443 EPI860496 HA A/tufted duck/Germany-SH/R8444/2016 1745 EPI860509 NP A/tufted duck/Germany-SH/R8444/2016 937 EPI860497 NA A/tufted duck/Germany-SH/R8444/2016 1410 EPI860510 MP A/tufted duck/Germany-SH/R8444/2016 1032 EPI860498 NS A/tufted duck/Germany-SH/R8444/2016 841 EPI860499 Submitter information Submitter: Starick, Elke Submission Date: 2016-11-18 Last modifier: Starick, Elke Last modified: 2016-11-18 Address: Friedrich-Loeffler-Institut Suedufer 10 17493 Greifswald-Insel Riems Germany

A/tufted duck/Germany-SH/R8444/2016 EPI_ISL_237958 H5N8 swab 2306 2294 1443 1745 937 1410 1032 841 --- --- 2016-11 A/tufted_duck/Germany/AR8459-L01988/2016 EPI_ISL_237945 H5N8 E1 2339 2336 2198 1764 1562 1450 1015 878 --- --- 2016-11-08 A/tufted_duck/Germany/AR8444-L01987/2016 EPI_ISL_237944 H5N8 E1 2320 2352 2208 1765 1555 1467 1016 880 --- --- 2016-11-07

FLI has released full H5N8 sequences (at GISAID) from Germany

Align Segment-ID Name Score E-Value Identity EPI873627 A/mute swan/Croatia/104/2016 (A/H5N8) segment 6 (NA) 2597.5 0.000000e+00 1406/1406 (100%) EPI869933 A/chicken/Poland/79A/2016 (A/H5N8) segment 6 (NA) 2580.9 0.000000e+00 1403/1406 (99%) EPI869811 A/duck/France/161108h/2016 (A/H5N8) segment 6 (NA) 2580.9 0.000000e+00 1403/1406 (99%) EPI873213 A/chicken/Croatia/103/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI869935 A/mute swan/Poland/108/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI869934 A/chicken/Poland/85A/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI861569 A/mute swan/Croatia/70/2016 (A/H5N8) segment 6 (NA) 2564.3 0.000000e+00 1400/1406 (99%) EPI864747 A/mute swan/Croatia/85/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI861573 A/mute swan/Croatia/78/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI873625 A/mute swan/Croatia/9/2017 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI869689 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI868850 A/turkey/England/052131/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI860232 A/wild duck/Poland/82A/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774500 A/Brown-headed Gull/Qinghai/ZTO6-MU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774468 A/Brown-headed Gull/Qinghai/ZTO5-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774452 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774444 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774436 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774420 A/Bar-headed Goose/Qinghai/BTY18-MU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774412 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774404 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774388 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774380 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774371 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774363 A/Bar-headed Goose/Qinghai/BTY15-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774346 A/Bar-headed Goose/Qinghai/BTY14-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774337 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774320 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774312 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774304 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774270 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774261 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774253 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774244 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774195 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774187 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774144 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774136 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774115 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI859649 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 6 (NA) 2532.9 0.000000e+00 1394/1406 (99%) EPI863867 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863851 A/Chicken/Sweden/SVA161122KU0453/SZ0209318/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863836 A/Chicken/Sweden/SVA161122KU0453/SZ0209317/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863828 A/Chicken/Sweden/SVA161122KU0453/SZ0209316/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI858846 A/painted stork/India/10CA03/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI836616 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI836608 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI823758 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI823750 A/wild duck/Tyva/35/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI823462 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774492 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774484 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774477 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1395/1407 (99%) EPI774460 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1395/1407 (99%) EPI774396 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1392/1403 (99%) EPI774354 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774328 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774296 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774279 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1392/1403 (99%) EPI774178 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774170 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774161 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863859 A/Chicken/Sweden/SVA161122KU0453/SZ0209321/2016 (A/H5N8) segment 6 (NA) 2527.3 0.000000e+00 1393/1406 (99%) EPI774508 A/Great Black-headed Gull/Qinghai/YO1-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774428 A/Brown-headed Gull/Qinghai/ZTO1-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774288 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774220 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774212 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1391/1403 (99%) EPI774152 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1394/1407 (99%) EPI773759 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI869932 A/domestic goose/Poland/72/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI869925 A/domestic goose/Poland/33/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI859213 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI774236 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1393/1407 (99%) EPI774228 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1392/1407 (98%) EPI858838 A/duck/India/10CA01/2016 (A/H5N8) segment 6 (NA) 2508.9 0.000000e+00 1390/1406 (98%) EPI774203 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 6 (NA) 2508.9 0.000000e+00 1390/1406 (98%) EPI703648 A/duck/Eastern China/S1109/2014 (A/H5N8) segment 6 (NA) 2486.7 0.000000e+00 1386/1406 (98%) EPI869941 A/herring gull/Poland/84/2016 (A/H5N8) segment 6 (NA) 2462.7 0.000000e+00 1357/1369 (99%) EPI703642 A/duck/Eastern China/S0215/2014 (A/H5N8) segment 6 (NA) 2453.5 0.000000e+00 1380/1406 (98%) EPI681357 A/goose/Zhejiang/925037/2014 (A/H5N8) segment 6 (NA) 2442.4 0.000000e+00 1378/1406 (98%) EPI681358 A/goose/Zhejiang/925104/2014 (A/H5N8) segment 6 (NA) 2420.2 0.000000e+00 1374/1406 (97%) EPI583873 A/duck/Shaoxing/5240/2013 (A/H0) segment 6 (NA) 2409.2 0.000000e+00 1372/1406 (97%) EPI591884 A/duck/Jiangxi/23970/2013 (A/H0) segment 6 (NA) 2403.6 0.000000e+00 1371/1406 (97%) EPI690794 A/goose/Jiangsu/QD5/2014 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1370/1406 (97%) EPI561493 A/Baikal teal/Korea/H52/2014 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1370/1406 (97%) EPI530990 A/duck/Zhejiang/6D18/2013 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1371/1407 (97%) EPI509700 A/breeder duck/Korea/Gochang1/2014 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1370/1406 (97%) EPI762160 A/goose/Eastern China/CZ/2013 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1369/1406 (97%) EPI703638 A/duck/Eastern China/S1210/2013 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1369/1406 (97%) EPI530989 A/duck/Zhejiang/W24/2013 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1370/1407 (97%) EPI681355 A/duck/Zhejiang/925019/2014 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1368/1406 (97%) EPI507675 A/mallard duck/Shanghai/SH-9/2013 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1369/1407 (97%) EPI762152 A/duck/Eastern China/JY/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1367/1406 (97%) EPI703635 A/duck/Eastern China/L1021/2012 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1367/1406 (97%) EPI690802 A/goose/Shandong/WFSG1/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1367/1406 (97%) EPI681356 A/duck/Zhejiang/925169/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1368/1407 (97%) EPI646030 A/goose/Jiangsu/WX202/2014 (A/H5N8) segment 6 (NA) 2375.9 0.000000e+00 1366/1406 (97%) EPI602039 A/duck/Ningbo/3262/2013 (A/H0) segment 6 (NA) 2374.1 0.000000e+00 1347/1378 (97%) EPI703634 A/duck/Eastern China/L0722/2012 (A/H5N8) segment 6 (NA) 2370.4 0.000000e+00 1365/1406 (97%

Align Segment-ID Name Score E-Value Identity EPI873627 A/mute swan/Croatia/104/2016 (A/H5N8) segment 6 (NA) 2597.5 0.000000e+00 1406/1406 (100%) EPI869933 A/chicken/Poland/79A/2016 (A/H5N8) segment 6 (NA) 2580.9 0.000000e+00 1403/1406 (99%) EPI869811 A/duck/France/161108h/2016 (A/H5N8) segment 6 (NA) 2580.9 0.000000e+00 1403/1406 (99%) EPI873213 A/chicken/Croatia/103/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI869935 A/mute swan/Poland/108/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI869934 A/chicken/Poland/85A/2016 (A/H5N8) segment 6 (NA) 2569.8 0.000000e+00 1401/1406 (99%) EPI861569 A/mute swan/Croatia/70/2016 (A/H5N8) segment 6 (NA) 2564.3 0.000000e+00 1400/1406 (99%) EPI864747 A/mute swan/Croatia/85/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI861573 A/mute swan/Croatia/78/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI873625 A/mute swan/Croatia/9/2017 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI869689 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI868850 A/turkey/England/052131/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI860232 A/wild duck/Poland/82A/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774500 A/Brown-headed Gull/Qinghai/ZTO6-MU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774468 A/Brown-headed Gull/Qinghai/ZTO5-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774452 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774444 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774436 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774420 A/Bar-headed Goose/Qinghai/BTY18-MU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774412 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774404 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774388 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774380 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774371 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774363 A/Bar-headed Goose/Qinghai/BTY15-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774346 A/Bar-headed Goose/Qinghai/BTY14-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774337 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774320 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774312 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774304 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774270 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774261 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774253 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774244 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774195 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774187 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774144 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774136 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI774115 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 6 (NA) 2536.6 0.000000e+00 1395/1406 (99%) EPI859649 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 6 (NA) 2532.9 0.000000e+00 1394/1406 (99%) EPI863867 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863851 A/Chicken/Sweden/SVA161122KU0453/SZ0209318/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863836 A/Chicken/Sweden/SVA161122KU0453/SZ0209317/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863828 A/Chicken/Sweden/SVA161122KU0453/SZ0209316/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI858846 A/painted stork/India/10CA03/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI836616 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI836608 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI823758 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI823750 A/wild duck/Tyva/35/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI823462 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774492 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774484 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774477 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1395/1407 (99%) EPI774460 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1395/1407 (99%) EPI774396 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1392/1403 (99%) EPI774354 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774328 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774296 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774279 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1392/1403 (99%) EPI774178 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774170 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774161 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI863859 A/Chicken/Sweden/SVA161122KU0453/SZ0209321/2016 (A/H5N8) segment 6 (NA) 2527.3 0.000000e+00 1393/1406 (99%) EPI774508 A/Great Black-headed Gull/Qinghai/YO1-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774428 A/Brown-headed Gull/Qinghai/ZTO1-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774288 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774220 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774212 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1391/1403 (99%) EPI774152 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1394/1407 (99%) EPI773759 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI869932 A/domestic goose/Poland/72/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI869925 A/domestic goose/Poland/33/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI859213 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI774236 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1393/1407 (99%) EPI774228 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1392/1407 (98%) EPI858838 A/duck/India/10CA01/2016 (A/H5N8) segment 6 (NA) 2508.9 0.000000e+00 1390/1406 (98%) EPI774203 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 6 (NA) 2508.9 0.000000e+00 1390/1406 (98%) EPI703648 A/duck/Eastern China/S1109/2014 (A/H5N8) segment 6 (NA) 2486.7 0.000000e+00 1386/1406 (98%) EPI869941 A/herring gull/Poland/84/2016 (A/H5N8) segment 6 (NA) 2462.7 0.000000e+00 1357/1369 (99%) EPI703642 A/duck/Eastern China/S0215/2014 (A/H5N8) segment 6 (NA) 2453.5 0.000000e+00 1380/1406 (98%) EPI681357 A/goose/Zhejiang/925037/2014 (A/H5N8) segment 6 (NA) 2442.4 0.000000e+00 1378/1406 (98%) EPI681358 A/goose/Zhejiang/925104/2014 (A/H5N8) segment 6 (NA) 2420.2 0.000000e+00 1374/1406 (97%) EPI583873 A/duck/Shaoxing/5240/2013 (A/H0) segment 6 (NA) 2409.2 0.000000e+00 1372/1406 (97%) EPI591884 A/duck/Jiangxi/23970/2013 (A/H0) segment 6 (NA) 2403.6 0.000000e+00 1371/1406 (97%) EPI690794 A/goose/Jiangsu/QD5/2014 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1370/1406 (97%) EPI561493 A/Baikal teal/Korea/H52/2014 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1370/1406 (97%) EPI530990 A/duck/Zhejiang/6D18/2013 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1371/1407 (97%) EPI509700 A/breeder duck/Korea/Gochang1/2014 (A/H5N8) segment 6 (NA) 2398.1 0.000000e+00 1370/1406 (97%) EPI762160 A/goose/Eastern China/CZ/2013 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1369/1406 (97%) EPI703638 A/duck/Eastern China/S1210/2013 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1369/1406 (97%) EPI530989 A/duck/Zhejiang/W24/2013 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1370/1407 (97%) EPI681355 A/duck/Zhejiang/925019/2014 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1368/1406 (97%) EPI507675 A/mallard duck/Shanghai/SH-9/2013 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1369/1407 (97%) EPI762152 A/duck/Eastern China/JY/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1367/1406 (97%) EPI703635 A/duck/Eastern China/L1021/2012 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1367/1406 (97%) EPI690802 A/goose/Shandong/WFSG1/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1367/1406 (97%) EPI681356 A/duck/Zhejiang/925169/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1368/1407 (97%) EPI646030 A/goose/Jiangsu/WX202/2014 (A/H5N8) segment 6 (NA) 2375.9 0.000000e+00 1366/1406 (97%) EPI602039 A/duck/Ningbo/3262/2013 (A/H0) segment 6 (NA) 2374.1 0.000000e+00 1347/1378 (97%) EPI703634 A/duck/Eastern China/L0722/2012 (A/H5N8) segment 6 (NA) 2370.4 0.000000e+00 1365/1406 (97%

Croatian Veterinary Institute Poultry Centre has release a full N8 sequence, A/mute swan/Croatia/104/2016, (at GISAID) from a mite swan at Mužilovčica Croatia.

Isolate name: A/mute swan/Croatia/104/2016 Isolate ID: EPI_ISL_240671 Passage details/history: 0 Type: A / H5N8 Lineage: Sample information Collection date: 2016-12-20 Host Cygnus olor Additional host information: Domestic status: Wild Is vaccinated: Location: Croatia / Sisacko-Moslavacka Zupanija Additional location information: Mužilovčica Health status: Dead Specimen source: Strain or commercial product name used for vaccination: Institute information Originating lab: Sample ID given by the sample provider: Address: Submitting lab: Croatian Veterinary Institute Sample ID given by the submitting laboratory: Authors: Savić, Vladimir Address: Croatian Veterinary Institute Poultry Centre Heinzelova 55 10000 Zagreb Croatia Publication Publication In vivo antiviral resistance Phenotype Genotype Unspecified Antiviral resistance tested by experimental procedures Adamantanes: Unknown Oseltamivir: Unknown Zanamivir: Unknown Peramivir: Unknown Other: Unknown Additional information Antigenic characterization: Note: Sequence segment identifier length accession # INSDC Sequence NA A/mute swan/Croatia/104/2016 1425 EPI873627 Submitter information Submitter: Savić, Vladimir Submission Date: 2017-01-07 Last modifier: Savić, Vladimir Last modified: 2017-01-07 Address: Croatian Veterinary Institute Poultry Centre Heinzelova 55 10000 Zagreb Croatia

Croatian Veterinary Institute Poultry Centre has release a full N8 sequence, A/mute swan/Croatia/104/2016, (at GISAID) from a mite swan at Mužilovčica Croatia.

Map Update https://www.google.com/maps/d/edit?hl=en&hl=en&authuser=0&authuser=0&mid=1aNOepBDPUd0zdRnQE1UbSW8djsk&ll=45.768354879846264%2C15.855674380493156&z=12

Align Segment-ID Name Score E-Value Identity EPI873625 A/mute swan/Croatia/9/2017 (A/H5N8) segment 6 (NA) 2597.5 0.000000e+00 1406/1406 (100%) EPI873213 A/chicken/Croatia/103/2016 (A/H5N8) segment 6 (NA) 2564.3 0.000000e+00 1400/1406 (99%) EPI869935 A/mute swan/Poland/108/2016 (A/H5N8) segment 6 (NA) 2564.3 0.000000e+00 1400/1406 (99%) EPI869933 A/chicken/Poland/79A/2016 (A/H5N8) segment 6 (NA) 2564.3 0.000000e+00 1400/1406 (99%) EPI869811 A/duck/France/161108h/2016 (A/H5N8) segment 6 (NA) 2564.3 0.000000e+00 1400/1406 (99%) EPI861569 A/mute swan/Croatia/70/2016 (A/H5N8) segment 6 (NA) 2558.7 0.000000e+00 1399/1406 (99%) EPI869934 A/chicken/Poland/85A/2016 (A/H5N8) segment 6 (NA) 2553.2 0.000000e+00 1398/1406 (99%) EPI873627 A/mute swan/Croatia/104/2016 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI864747 A/mute swan/Croatia/85/2016 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI861573 A/mute swan/Croatia/78/2016 (A/H5N8) segment 6 (NA) 2547.7 0.000000e+00 1397/1406 (99%) EPI869689 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 6 (NA) 2542.1 0.000000e+00 1396/1406 (99%) EPI868850 A/turkey/England/052131/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI860232 A/wild duck/Poland/82A/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774500 A/Brown-headed Gull/Qinghai/ZTO6-MU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774468 A/Brown-headed Gull/Qinghai/ZTO5-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774452 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774444 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774436 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774420 A/Bar-headed Goose/Qinghai/BTY18-MU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774412 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774404 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774388 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774380 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774371 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774363 A/Bar-headed Goose/Qinghai/BTY15-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774346 A/Bar-headed Goose/Qinghai/BTY14-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774337 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774320 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774312 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774304 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774270 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774261 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774253 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774244 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774195 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774187 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774144 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774136 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI774115 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 6 (NA) 2531.0 0.000000e+00 1394/1406 (99%) EPI859649 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 6 (NA) 2527.3 0.000000e+00 1393/1406 (99%) EPI863867 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI863851 A/Chicken/Sweden/SVA161122KU0453/SZ0209318/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI863836 A/Chicken/Sweden/SVA161122KU0453/SZ0209317/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI863828 A/Chicken/Sweden/SVA161122KU0453/SZ0209316/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI858846 A/painted stork/India/10CA03/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1394/1407 (99%) EPI836616 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI836608 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI823758 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI823750 A/wild duck/Tyva/35/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI823462 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774492 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774484 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774477 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1394/1407 (99%) EPI774460 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1394/1407 (99%) EPI774396 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1391/1403 (99%) EPI774354 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774328 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774296 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774279 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1391/1403 (99%) EPI774178 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774170 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI774161 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 6 (NA) 2525.5 0.000000e+00 1393/1406 (99%) EPI863859 A/Chicken/Sweden/SVA161122KU0453/SZ0209321/2016 (A/H5N8) segment 6 (NA) 2521.8 0.000000e+00 1392/1406 (99%) EPI774508 A/Great Black-headed Gull/Qinghai/YO1-B/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI774428 A/Brown-headed Gull/Qinghai/ZTO1-B/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI774288 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI774220 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI774212 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1390/1403 (99%) EPI774152 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1393/1407 (99%) EPI773759 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 6 (NA) 2520.0 0.000000e+00 1392/1406 (99%) EPI869932 A/domestic goose/Poland/72/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1391/1406 (98%) EPI869925 A/domestic goose/Poland/33/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1391/1406 (98%) EPI859213 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1391/1406 (98%) EPI774236 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 6 (NA) 2514.4 0.000000e+00 1392/1407 (98%) EPI774228 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 6 (NA) 2508.9 0.000000e+00 1391/1407 (98%) EPI858838 A/duck/India/10CA01/2016 (A/H5N8) segment 6 (NA) 2503.3 0.000000e+00 1389/1406 (98%) EPI774203 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 6 (NA) 2503.3 0.000000e+00 1389/1406 (98%) EPI703648 A/duck/Eastern China/S1109/2014 (A/H5N8) segment 6 (NA) 2481.2 0.000000e+00 1385/1406 (98%) EPI869941 A/herring gull/Poland/84/2016 (A/H5N8) segment 6 (NA) 2457.2 0.000000e+00 1356/1369 (99%) EPI703642 A/duck/Eastern China/S0215/2014 (A/H5N8) segment 6 (NA) 2447.9 0.000000e+00 1379/1406 (98%) EPI681357 A/goose/Zhejiang/925037/2014 (A/H5N8) segment 6 (NA) 2436.9 0.000000e+00 1377/1406 (97%) EPI681358 A/goose/Zhejiang/925104/2014 (A/H5N8) segment 6 (NA) 2414.7 0.000000e+00 1373/1406 (97%) EPI583873 A/duck/Shaoxing/5240/2013 (A/H0) segment 6 (NA) 2403.6 0.000000e+00 1371/1406 (97%) EPI591884 A/duck/Jiangxi/23970/2013 (A/H0) segment 6 (NA) 2398.1 0.000000e+00 1370/1406 (97%) EPI690794 A/goose/Jiangsu/QD5/2014 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1369/1406 (97%) EPI561493 A/Baikal teal/Korea/H52/2014 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1369/1406 (97%) EPI530990 A/duck/Zhejiang/6D18/2013 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1370/1407 (97%) EPI509700 A/breeder duck/Korea/Gochang1/2014 (A/H5N8) segment 6 (NA) 2392.5 0.000000e+00 1369/1406 (97%) EPI762160 A/goose/Eastern China/CZ/2013 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1368/1406 (97%) EPI703638 A/duck/Eastern China/S1210/2013 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1368/1406 (97%) EPI530989 A/duck/Zhejiang/W24/2013 (A/H5N8) segment 6 (NA) 2387.0 0.000000e+00 1369/1407 (97%) EPI681355 A/duck/Zhejiang/925019/2014 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1367/1406 (97%) EPI507675 A/mallard duck/Shanghai/SH-9/2013 (A/H5N8) segment 6 (NA) 2381.5 0.000000e+00 1368/1407 (97%) EPI703635 A/duck/Eastern China/L1021/2012 (A/H5N8) segment 6 (NA) 2375.9 0.000000e+00 1366/1406 (97%) EPI690802 A/goose/Shandong/WFSG1/2014 (A/H5N8) segment 6 (NA) 2375.9 0.000000e+00 1366/1406 (97%) EPI681356 A/duck/Zhejiang/925169/2014 (A/H5N8) segment 6 (NA) 2375.9 0.000000e+00 1367/1407 (97%) EPI646030 A/goose/Jiangsu/WX202/2014 (A/H5N8) segment 6 (NA) 2370.4 0.000000e+00 1365/1406 (97%) EPI602039 A/duck/Ningbo/3262/2013 (A/H0) segment 6 (NA) 2368.5 0.000000e+00 1346/1378 (97%) EPI762152 A/duck/Eastern China/JY/2014 (A/H5N8) segment 6 (NA) 2364.8 0.000000e+00 1364/1406 (97%) EPI703634 A/duck/Eastern China/L0722/2012 (A/H5N8) segment 6 (NA) 2364.8 0.000000e+00 1364/1406 (97%)

Align Segment-ID Name Score E-Value Identity EPI873624 A/mute swan/Croatia/9/2017 (A/H5N8) segment 4 (HA) 3147.8 0.000000e+00 1704/1704 (100%) EPI869929 A/mute swan/Poland/108/2016 (A/H5N8) segment 4 (HA) 3142.3 0.000000e+00 1703/1704 (99%) EPI869809 A/duck/France/161108h/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI864746 A/mute swan/Croatia/85/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI861568 A/mute swan/Croatia/70/2016 (A/H5N8) segment 4 (HA) 3136.7 0.000000e+00 1702/1704 (99%) EPI861572 A/mute swan/Croatia/78/2016 (A/H5N8) segment 4 (HA) 3131.2 0.000000e+00 1701/1704 (99%) EPI869931 A/turkey/Poland/83/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI869927 A/chicken/Poland/79A/2016 (A/H5N8) segment 4 (HA) 3120.1 0.000000e+00 1699/1704 (99%) EPI869930 A/turkey/Poland/78/2016 (A/H5N8) segment 4 (HA) 3116.4 0.000000e+00 1698/1704 (99%) EPI869928 A/chicken/Poland/85A/2016 (A/H5N8) segment 4 (HA) 3114.6 0.000000e+00 1698/1704 (99%) EPI873212 A/chicken/Croatia/103/2016 (A/H5N8) segment 4 (HA) 3099.8 0.000000e+00 1694/1702 (99%) EPI869924 A/domestic goose/Poland/33/2016 (A/H5N8) segment 4 (HA) 3099.8 0.000000e+00 1695/1704 (99%) EPI869926 A/domestic goose/Poland/72/2016 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI869687 A/decoy_duck/France/161104e/2016 (A/H5N8) segment 4 (HA) 3088.7 0.000000e+00 1693/1704 (99%) EPI869940 A/herring gull/Poland/84/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI868848 A/turkey/England/052131/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI859212 A/tufted_duck/Germany-SH/R8446/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI836614 A/common tern /Uvs-Nuur Lake/26/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI836606 A/grey heron /Uvs-Nuur Lake/20/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI823756 A/black-headed gull/Tyva/41/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI823748 A/wild duck/Tyva/35/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI823460 A/great crested grebe/Tyva/34/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774394 A/Bar-headed Goose/Qinghai/BTY17-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774386 A/Bar-headed Goose/Qinghai/BTY17-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774310 A/Bar-headed Goose/Qinghai/BTY12-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774242 A/Bar-headed Goose/Qinghai/BTY8-LU/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774234 A/Bar-headed Goose/Qinghai/BTY8-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774121 A/Bar-headed Goose/Qinghai/BTY1-LV/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI774113 A/Bar-headed Goose/Qinghai/BTY1-B/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1693/1704 (99%) EPI773757 A/great crested grebe/Uvs-Nuur Lake/341/2016 (A/H5N8) segment 4 (HA) 3086.9 0.000000e+00 1694/1705 (99%) EPI859650 A/wild duck/Germany-BW/R8455/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774475 A/Brown-headed Gull/Qinghai/ZTO5-K/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774458 A/Brown-headed Gull/Qinghai/ZTO4-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774450 A/Brown-headed Gull/Qinghai/ZTO3-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774402 A/Bar-headed Goose/Qinghai/BTY18-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774378 A/Bar-headed Goose/Qinghai/BTY16-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774326 A/Bar-headed Goose/Qinghai/BTY13-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774318 A/Bar-headed Goose/Qinghai/BTY13-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774302 A/Bar-headed Goose/Qinghai/BTY12-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774277 A/Bar-headed Goose/Qinghai/BTY10-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774267 A/Bar-headed Goose/Qinghai/BTY10-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774259 A/Bar-headed Goose/Qinghai/BTY9-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774251 A/Bar-headed Goose/Qinghai/BTY9-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774226 A/Bar-headed Goose/Qinghai/BTY7-LU2/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774218 A/Bar-headed Goose/Qinghai/BTY7-LU1/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774210 A/Bar-headed Goose/Qinghai/BTY7-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774201 A/Bar-headed Goose/Qinghai/BTY6-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774193 A/Bar-headed Goose/Qinghai/BTY6-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774185 A/Bar-headed Goose/Qinghai/BTY5-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774176 A/Bar-headed Goose/Qinghai/BTY4-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774168 A/Bar-headed Goose/Qinghai/BTY4-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774159 A/Bar-headed Goose/Qinghai/BTY3-LU/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774150 A/Bar-headed Goose/Qinghai/BTY3-B/2016 (A/H5N8) segment 4 (HA) 3081.3 0.000000e+00 1692/1704 (99%) EPI774506 A/Great Black-headed Gull/Qinghai/YO1-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774490 A/Brown-headed Gull/Qinghai/ZTO6-SP/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774483 A/Brown-headed Gull/Qinghai/ZTO6-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774434 A/Brown-headed Gull/Qinghai/ZTO1-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774410 A/Bar-headed Goose/Qinghai/BTY18-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774369 A/Bar-headed Goose/Qinghai/BTY16-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774335 A/Bar-headed Goose/Qinghai/BTY14-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774294 A/Bar-headed Goose/Qinghai/BTY11-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774286 A/Bar-headed Goose/Qinghai/BTY11-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774142 A/Bar-headed Goose/Qinghai/BTY2-LU/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI774133 A/Bar-headed Goose/Qinghai/BTY2-B/2016 (A/H5N8) segment 4 (HA) 3075.8 0.000000e+00 1691/1704 (99%) EPI863865 A/Common Goldeneye/Sweden/SVA161117KU0322/SZ0002165/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI858844 A/painted stork/India/10CA03/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774466 A/Brown-headed Gull/Qinghai/ZTO5-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI774442 A/Brown-headed Gull/Qinghai/ZTO3-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1691/1705 (99%) EPI774352 A/Bar-headed Goose/Qinghai/BTY15-B/2016 (A/H5N8) segment 4 (HA) 3070.3 0.000000e+00 1690/1704 (99%) EPI858836 A/duck/India/10CA01/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI774498 A/Brown-headed Gull/Qinghai/ZTO6-MU/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI774426 A/Brown-headed Gull/Qinghai/ZTO1-B/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI774361 A/Bar-headed Goose/Qinghai/BTY15-LU/2016 (A/H5N8) segment 4 (HA) 3064.7 0.000000e+00 1689/1704 (99%) EPI860231 A/wild duck/Poland/82A/2016 (A/H5N8) segment 4 (HA) 3061.0 0.000000e+00 1688/1704 (99%) EPI774344 A/Bar-headed Goose/Qinghai/BTY14-LU/2016 (A/H5N8) segment 4 (HA) 3059.2 0.000000e+00 1688/1704 (99%) EPI863857 A/Chicken/Sweden/SVA161122KU0453/SZ0209321/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI863849 A/Chicken/Sweden/SVA161122KU0453/SZ0209318/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI863834 A/Chicken/Sweden/SVA161122KU0453/SZ0209317/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI863826 A/Chicken/Sweden/SVA161122KU0453/SZ0209316/2016 (A/H5N8) segment 4 (HA) 3053.6 0.000000e+00 1687/1704 (99%) EPI703608 A/duck/Eastern China/S1109/2014 (A/H5N8) segment 4 (HA) 3048.1 0.000000e+00 1686/1704 (98%) EPI681300 A/goose/Zhejiang/925104/2014 (A/H5N8) segment 4 (HA) 2981.6 0.000000e+00 1674/1704 (98%) EPI681299 A/goose/Zhejiang/925037/2014 (A/H5N8) segment 4 (HA) 2981.6 0.000000e+00 1674/1704 (98%) EPI703602 A/duck/Eastern China/S0215/2014 (A/H5N8) segment 4 (HA) 2970.5 0.000000e+00 1672/1704 (98%) EPI675774 A/duck/Liaoning/S1001/2014 (A/H5N8) segment 4 (HA) 2970.5 0.000000e+00 1672/1704 (98%) EPI681290 A/goose/Zhejiang/925106/2014 (A/H5N6) segment 4 (HA) 2959.5 0.000000e+00 1670/1704 (98%) EPI509698 A/breeder duck/Korea/Gochang1/2014 (A/H5N8) segment 4 (HA) 2948.4 0.000000e+00 1668/1704 (97%) EPI561495 A/Baikal teal/Korea/H52/2014 (A/H5N8) segment 4 (HA) 2937.3 0.000000e+00 1666/1704 (97%) EPI591879 A/duck/Jiangxi/23970/2013 (A/H0) segment 4 (HA) 2931.8 0.000000e+00 1665/1704 (97%) EPI703599 A/duck/Eastern China/S1210/2013 (A/H5N8) segment 4 (HA) 2926.2 0.000000e+00 1664/1704 (97%) EPI690792 A/goose/Jiangsu/QD5/2014 (A/H5N8) segment 4 (HA) 2926.2 0.000000e+00 1664/1704 (97%) EPI762158 A/goose/Eastern China/CZ/2013 (A/H5N8) segment 4 (HA) 2920.7 0.000000e+00 1663/1704 (97%) EPI703607 A/goose/Eastern China/S0408/2014 (A/H5N8) segment 4 (HA) 2920.7 0.000000e+00 1663/1704 (97%) EPI690808 A/goose/Yangzhou/0420/2014 (A/H5N8) segment 4 (HA) 2920.7 0.000000e+00 1664/1705 (97%) EPI690800 A/goose/Shandong/WFSG1/2014 (A/H5N8) segment 4 (HA) 2920.7 0.000000e+00 1663/1704 (97%) EPI602037 A/duck/Ningbo/3262/2013 (A/H0) segment 4 (HA) 2915.1 0.000000e+00 1662/1704 (97%) EPI583870 A/duck/Shaoxing/5240/2013 (A/H0) segment 4 (HA) 2915.1 0.000000e+00 1662/1704 (97%) EPI530985 A/duck/Zhejiang/W24/2013 (A/H5N8) segment 4 (HA) 2915.1 0.000000e+00 1662/1704 (97%) EPI681280 A/goose/Zhejiang/77166/2014 (A/H5N2) segment 4 (HA) 2911.4 0.000000e+00 1660/1702 (97%) EPI762150 A/duck/Eastern China/JY/2014 (A/H5N8) segment 4 (HA) 2909.6 0.000000e+00 1662/1705 (97%) EPI530986 A/duck/Zhejiang/6D18/2013 (A/H5N8) segment 4 (HA) 2909.6 0.000000e+00 1661/1704 (97%

Isolate name: A/mute swan/Croatia/9/2017 Isolate ID: EPI_ISL_240670 Passage details/history: 0 Type: A / H5N8 Lineage: Sample information Collection date: 2017-01-03 Host Cygnus olor Additional host information: Zagreb, Jarun Lake Domestic status: Wild Is vaccinated: Location: Croatia / Grad Zagreb Additional location information: Health status: Dead Specimen source: Organ Strain or commercial product name used for vaccination: Institute information Originating lab: Sample ID given by the sample provider: Address: Submitting lab: Croatian Veterinary Institute Sample ID given by the submitting laboratory: Authors: Savić, Vladimir Address: Croatian Veterinary Institute Poultry Centre Heinzelova 55 10000 Zagreb Croatia Publication Publication In vivo antiviral resistance Phenotype Genotype Unspecified Antiviral resistance tested by experimental procedures Adamantanes: Unknown Oseltamivir: Unknown Zanamivir: Unknown Peramivir: Unknown Other: Unknown Additional information Antigenic characterization: Note: Sequence segment identifier length accession # INSDC Sequence HA A/mute swan/Croatia/9/2017 1736 EPI873624 NA A/mute swan/Croatia/9/2017 1425 EPI873625 Submitter information Submitter: Savić, Vladimir Submission Date: 2017-01-07 Last modifier: Savić, Vladimir Last modified: 2017-01-07 Address: Croatian Veterinary Institute Poultry Centre Heinzelova 55 10000 Zagreb Croatia

Croatian Veterinary Institute Poultry Centr release full 2017 H5 and N8 sequences, A/mute swan/Croatia/9/2017, (at GISAID) from mute swan at Jarun Lake in Croatia.

1 Petersen EE, Polen KN, Meaney-Delman D, et al. Update: interim guidance for health care providers caring for women of reproductive age with possible Zika virus exposure — United States, 2016. MMWR Morb Mortal Wkly Rep 2016;65:315-322 CrossRef | Web of Science | Medline 2 Driggers RW, Ho C-Y, Korhonen EM, et al. Zika virus infection with prolonged maternal viremia and fetal brain abnormalities. N Engl J Med 2016;374:2142-2151 Free Full Text | Web of Science | Medline 3 Martínez de Salazar P, Suy A, Sánchez-Montalvá A, Rodó C, Salvador F, Molina I. Zika fever. Enferm Infecc Microbiol Clin 2016;34:247-252 CrossRef | Web of Science | Medline

To the Editor: We describe a case of Zika virus (ZIKV) infection during pregnancy in a Colombian woman. She was infected in December 2015 while she was visiting her home country. At 9 weeks’ gestation, she had a self-limited maculopapular, nonconfluent rash for 3 days that affected her trunk, arms, and legs; she had no fever or other concurrent symptoms. She was screened for ZIKV and other flaviviruses. A reverse-transcriptase–polymerase-chain-reaction (RT-PCR) assay (RealStar Zika Virus RT-PCR Kit 1.0, Altona Diagnostics) of a serum sample was positive for ZIKV, and testing remained positive for 89 days (or 107 days after the onset of symptoms, until 29 weeks’ gestation), in six consecutive blood samples. Testing for ZIKV in the urine, vagina, and endocervix was negative. No fetal brain abnormalities were observed on scans obtained at 12 and 15 weeks’ gestation. Neurosonography performed at 20, 24, and 29 weeks’ gestation revealed bilateral mild ventriculomegaly and a shortened corpus callosum. The posterior fossa was normal. The brain parenchyma had calcifications and severe atrophy. Similar findings were seen on magnetic resonance imaging (MRI). No other anomalies were found in the fetus or the placenta. An RT-PCR assay of the amniotic fluid was positive for ZIKV, and screening was negative for dengue virus, chikungunya virus, cytomegalovirus, varicella–zoster virus, parvovirus B19, Toxoplasma gondii, and sexually transmitted infectious agents (Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Ureaplasma parvum, M. genitalium, U. urealiticum, and Trichomonas vaginalis). The ZIKV viral load in the amniotic fluid was higher than that in the maternal serum (cycle threshold values, 28 vs. 35; RT-PCR cycle threshold values were used as an indirect marker of viral load). Results of targeted genetic testing of the amniotic fluid by means of microarray-based comparative genomic hybridization (SurePrint G3 Unrestricted CGH ISCA v2 Microarray Kit, 8x60K, Agilent Technologies) were normal. The baby was delivered at 37 weeks’ gestation because of suspected growth restriction. At this time, RT-PCR assays of the maternal serum, urine, amniotic fluid, placenta, membranes, and umbilical cord were negative for ZIKV. RT-PCR assays of the neonatal serum, urine, and cerebrospinal fluid were also negative. Postnatal ultrasonography and MRI studies confirmed the presence of microcephaly with a thinned corpus callosum and brain atrophy with parenchymal calcifications. (Table 1TABLE 1Patient Data. shows the evolution of laboratory and ultrasonographic findings in the mother and the baby.) ZIKV has been documented to be detectable in maternal blood by means of molecular techniques during the acute phase of the infection (the first 5 days after the onset of clinical symptoms).1 Driggers et al. (June 2 issue)2detected ZIKV RNA in maternal serum 8 weeks after the onset of clinical symptoms. They suggested that persistent viremia may occur as a consequence of viral replication in the fetus or placenta. In our case, certain findings would support this hypothesis. First, the viral load in the amniotic fluid was higher than that in the maternal serum. Second, the viral load in the maternal serum remained stable (cycle threshold value, approximately 35) for 14 weeks and then became negative, instead of decreasing progressively, as would be expected. Third, neutralizing antibodies and ZIKV RNA were present in the maternal serum. In addition, RT-PCR assays of the maternal urine were negative, while testing of the maternal serum was positive. According to previous studies,3 ZIKV RNA would be detectable in urine longer than in serum, so an RT-PCR assay of the maternal urine would be expected to be positive in the presence of maternal viremia. For all these reasons, we would hypothesize that the persistent viremia that was detected in the mother could be the result of viral replication in the fetus or placenta, which thus acts as a reservoir. We presume that the RT-PCR testing of neonatal samples was negative because the clinical infection occurred during prenatal life; hence, it is possible that ZIKV antibodies developed in the baby’s immune system before birth. In summary, persistent ZIKV RNA in maternal serum could be a sign of fetal infection, and thus the fetus may play a role in persistent maternal viremia.

Anna Suy, M.D., Ph.D. Hospital Vall d’Hebron, Barcelona, Spain [email protected] Elena Sulleiro, M.D. Carlota Rodó, M.D. Universitat Autònoma de Barcelona, Barcelona, Spain Élida Vázquez, M.D. Cristina Bocanegra, M.D. Israel Molina, M.D., Ph.D. Juliana Esperalba, M.D. Hospital Vall d’Hebron, Barcelona, Spain María P. Sánchez-Seco, M.D., Ph.D. Centro Nacional de Microbiología, Madrid, Spain Hector Boix, M.D. Tomás Pumarola, M.D., Ph.D. Elena Carreras, M.D., Ph.D. Hospital Vall d’Hebron, Barcelona, Spain Disclosure forms provided by the authors are available with the full text of this letter at NEJM.org. Drs. Suy, Sulleiro, and Rodó contributed equally to this letter. This letter was published on December 7, 2016, at NEJM.org.

More evidence of Zika in serum due to fetal reservoir - NEJM Prolonged Zika Virus Viremia during Pregnancy N Engl J Med 2016; 375:2611-2613December 29, 2016DOI: 10.1056/NEJMc1607580 http://www.nejm.org/doi/full/10.1056/NEJMc1607580

Conclusions A new reassortant influenza A(H5N8) virus is responsible for the recent HPAIV outbreak in Germany. The observed differences in pathogenicity for a broad spectrum of waterfowl compared with that of H5N8 viruses from 2014–2015 correlate with a new genome composition of these viruses. The novel NP and PA segments in the 2016 H5N8 viruses from Germany are candidates for future studies of the molecular basis of the biological differences. These new strains may be more efficiently transmitted by and shed to other wild and domestic birds, a hypothesis in line with the large number of cases among wild birds in November 2016. There is yet no indication that mammals (including humans) are infected by these novel strains. Future studies in mammalian models (e.g., ferrets, mice) will provide experimental data on the virulence for mammals. Dr. Pohlmann is a microbiologist and senior scientist working at the Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Insel Riems, Germany. Her research interests are focused on influenza viruses, sequence analysis, phylogeny, and next-generation sequencing. Acknowledgments We gratefully acknowledge professional support from local veterinarians, diagnosticians in regional laboratories in Neumünster (Schleswig-Holstein), Rostock (Mecklenburg-Western Pomerania), Aulendorf and Freiburg (Baden-Wuerttemberg), and technical assistance by Diana Wessler, Aline Maksimov, Nicole Reimer, Kathrin Steffen, Patrick Zitzow, Bianka Hillmann, Jörn Gethmann, the Tierseuchennachrichtensystem (national animal disease notification system) team, and Cornelia Illing. This work was financed in part by COMPARE, under the European Union Horizon 2020 program (grant agreement no. 643476.)

Figure 2 Figure 2. Proposed reassortment events leading to the novel central Europe HPAIV A(H5N8) clade 2.3.4.4 virus. The Russia–Mongolia reassortant clade 2.3.4.4 H5N8 virus acquired 2 new segments (polymerase acidic protein and nucleoprotein), leading to the novel central Europe clade 2.3.4.4 H5N8 in 2016. Similar segment origins are marked by similar colors. Dashed lines indicate putative precursors. HPAIV, highly pathogenic avian influenza virus; LPAI, low pathogenicity avian influenza virus.

Figure 1 Figure 1. Highly pathogenic avian influenza A(H5N8) cases in wild birds and outbreaks in poultry holdings (10 backyard holdings, 4 zoos or pet farms, and a few commercial operations) in Germany, November 2016. Circles indicate original locations of outbreaks and isolates.

The Study Figure 1. Highly pathogenic avian influenza A(H5N8) cases in wild birds and outbreaks in poultry holdings (10 backyard holdings, 4 zoos or pet farms, and a few commercial operations) in Germany, November 2016.... In late May 2016, a group B clade 2.3.4.4 H5N8 virus was detected in dead and hunted wild birds at Lake Uvs-Nuur, at the Russia–Mongolia border (5). On November 7, 2016, many dead tufted ducks (Aythya fuligula) were found at Lake Plön in Schleswig-Holstein, northern Germany, and at Lake Constance in Baden-Württemberg, southern Germany (Figure 1); most were positive for H5N8. The epidemic among wild birds continued and spread toward the center of the country (Figure 1). As of December 2016, several backyard holdings, 4 zoos, and a few large commercial operations were also affected. Direct or indirect contact with wild birds was the most likely route of virus introduction into the backyard holdings and zoos. Despite the generally high standards of commercial operations, possible biosecurity gaps were identified. Most affected birds were found dead or exhibited severe clinical signs such as apathy or sudden deaths (in some parts of the affected chicken breeder farms, up to 90% died before culling). Macroscopic changes commonly observed in tufted ducks and poultry included severe diffuse hepatic necrosis, multifocal petechiae, and variably hyperemic and edematous lungs. Light microscopy confirmed influenza A virus nucleoprotein (NP) antigen and variably distinct necrotizing lesions in liver, heart, lungs, brain, pancreas, spleen, and thymus (Technical Appendix[PDF - 661 KB - 4 pages] Figure 1). Some chickens also displayed severe diffuse catarrhal enterocolitis; influenza A virus NP antigen was present in the intestinal epithelium. The spectrum of affected species of wild birds is broad and includes mainly diving ducks but also swans, grebes, gulls, buzzards, crows, and a white-tailed eagle (Table 1). As of November 30, 2016, ≈400 infected wild birds were detected in 13 federal states of Germany. H5N8 infections were also reported from Austria, Croatia, Denmark, Finland, France, Hungary, Poland, Romania, Sweden, Switzerland, and the Netherlands, indicating that the same subtype caused the recent outbreaks throughout Europe. The high pathogenicity for gallinaceous poultry was confirmed; intravenous pathogenicity index for 1 isolate (A/tufted duck/Germany-SH/AR8444/2016) was 2.93, comparable to the 2.81 index for H5N8 circulating in 2014 (A/turkey/Germany-MV/AR2472/2014). However, deaths of wild birds of a variety of species, in particular diving ducks, and extended pathologic changes in dead wild birds suggested a marked shift of pathogenicity from the viruses in Germany in 2014 (1). For genetic characterization, we analyzed virus sequences from several swab samples and the first 2 virus isolates from dead tufted ducks from Lake Plön and Lake Constance and compared them with sequences from H5N8-positive turkeys and chickens. We found few genetic differences between the analyzed strains from northern and southern Germany and only slight differences between sequences generated from wild bird or poultry samples. This finding clearly contrasts with those of the H5N1 clade 2.2 outbreaks in Germany in 2006, which showed similar distribution (6) but 2 distinct H5N1 northern and southern subclusters and a difference in timing during the first transcontinental wave; virus was found in Europe in October 2005 but not in Germany until early 2006. Figure 2. Proposed reassortment events leading to the novel central Europe HPAIV A(H5N8) clade 2.3.4.4 virus. The Russia–Mongolia reassortant clade 2.3.4.4 H5N8 virus acquired 2 new segments (polymerase acidic protein and nucleoprotein), leading... All genome segments of the novel H5N8 clade 2.3.4.4 group B strains from Germany in 2016 differed significantly from the H5N8 clade 2.3.4.4 group A strains detected in 2014–2015 in Germany and other European countries (1). This finding is in accord with findings of studies suggesting that there was no continued circulation of group A–like viruses among wild birds in the Netherlands from mid-November 2014 to January 2016 (7). Database searches identified H5N8 clade 2.3.4.4 viruses (first detected in wild birds at the Russia–Mongolia border at the end of May 2016) as closest relatives. Extended searches confirmed that the isolates from Germany in 2016 described in this study are novel reassortants, which can be clearly distinguished from the isolates from Russia–Mongolia in 2016 (e.g., A/great crested grebe/Uvs-Nuur Lake/341/2016) by 2 segments (Table 2; Technical Appendix[PDF - 661 KB - 4 pages] Figure 2). Six segments (polymerase basic [PB] 2, PB1, hemagglutinin, neuraminidase, matrix protein, nonstructural protein [NS]) were highly similar to those of clade 2.3.4.4 viruses from Russia in 2016 (99% for each surface protein; Table 2). The NS1 protein of the new 2016 isolate from Germany is truncated (217 aa) compared with the Russia–Mongolia viruses (230 aa), a truncation found in other influenza virus strains. However, nuclear export protein is not affected. Of note, the Russia–Mongolia virus proved to be a novel reassortant from earlier H5N8 clade 2.3.4.4 viruses within group B (Table 2; Figure 2) (5). These new H5N8 viruses are central Asia reassortants, which originated from strains circulating in eastern Asia. Genes of 3 segments (hemagglutinin, neuraminidase, and NS protein) cluster with segments of H5N8 clade 2.3.4.4 group B viruses identified in eastern China; the other 5 genes (PB1, PB2, polymerase acidic [PA], nucleoprotein [NP], and matrix protein) cluster with avian influenza viruses of low pathogenicity, which were identified in Mongolia, China, and Vietnam (5). The viruses from Germany (2016) further evolved from the strains from Russia and harbor 2 new segments (PA, NP). The virus sequences are essentially identical to sequences from an H5N8 virus isolate found November 2016 in a dead wild duck in Poland near the Germany border (Table 2) but differ significantly from sequences of other currently circulating isolates (Technical Appendix[PDF - 661 KB - 4 pages] Figure 2). The new PA sequences cluster with sequences of viruses detected in eastern and central Asia. The NP segment sequences are similar to those of viruses frequently found in central and northwestern Europe. The PA and NP segments in question occurred concurrently in different avian influenza viruses before; namely, in H6N8 strains from Iceland in 2011 (e.g., A/greylag goose/Iceland/0921/2011) and in an H6N2 isolate from Georgia in 2011 (A/mallard/Republic of Georgia/13/2011) (Table 2; Technical Appendix[PDF - 661 KB - 4 pages]Figure 2). It is reasonable to suggest that the novel reassortant strain from Germany was generated by >1 reassortment event that occurred during June–November 2016 between central Asia (Mongolia) and central Europe (Poland/Germany).